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wash buffer  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology wash buffer
    Wash Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+buffer/FCM+Wash+buffer/pm26324782-82-9-38
    Average 93 stars, based on 22 article reviews
    wash buffer - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Anti-invasive and anti-adhesive activities of a recombinant disintegrin, r-viridistatin 2, derived from the Prairie rattlesnake ( Crotalus viridis viridis )
    Article Snippet: .. After blocking, cells were washed with 1 mL 1× PBS, centrifuged, and resuspended in FCM Wash Buffer (Santa Cruz Biotechnology) to 10 6 cells/mL. ..

    Article Title: Functional characterization of six aspartate (D) recombinant mojastin mutants (r-Moj): a second aspartate amino acid carboxyl to the RGD in r-Moj-D_ peptides is not sufficient to induce apoptosis of SK-Mel-28 cells
    Article Snippet: .. After blocking, cells were washed with 1mL 1× PBS, centrifuged, and resuspended in FCM Wash Buffer (Santa Cruz Biotechnology) to 10 6 cells/mL. ..

    Flow Cytometry:

    Article Title: Uropathogenic Escherichia coli Engages CD14-Dependent Signaling to Enable Bladder-Macrophage-Dependent Control of Acute Urinary Tract Infection.
    Article Snippet: .. Cells were centrifuged and resuspended in 200 μL of wash buffer; cells were counted and placed in flow cytometry tubes, with 106 cells per tube, and stained with anti-mouse F4/80 allophycocyanin-conjugated antibody (eBiosciences) and anti-mouse CD14 primary antibody (Santa Cruz, Dallas, Texas), followed by secondary anti-goat–phycoerythrin antibody (BD Biosciences), for 30 minutes each in the dark (antibodies were used at the concentrations recommended by the manufacturers). .. Cells were washed, resuspended in 4% paraformaldehyde, and analyzed the following day by using a LSRII Fortessa flow cytometer (BD Bioscience, North Ryde, Australia).

    Article Title: CRISPR-Cas9-Mediated Correction of SLC12A3 Gene Mutation Rescues the Gitelman's Disease Phenotype in a Patient-Derived Kidney Organoid System.
    Article Snippet: .. 2023, 24, 3019 11 of 14 serum albumin and 10 mM sodium azide), permeabilized for 30 min using flow cytometry fixation and permeabilization solution (554714; BD Biosciences, San Jose, CA, USA), washed with wash buffer, and stained with stage-specific embryonic antigens SSEA-4 (813-70, 1:100; Santa Cruz Biotechnology, Dallas TX, USA) or TRA-1–81 (TRA-1-81, 1:100; Santa Cruz Biotechnology) surface antibodies for 30 min. Intracellular staining for NANOG (1E6C4, 1:100; Santa Cruz Biotechnology) was performed by sequential incubations with fixation and permeabilization solutions (A and B Fix & Perm Solutions, Invitrogen, BD Bioscience). .. These cells were incubated with FITC-conjugated secondary antibody (BD Bioscience).

    Article Title: Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics
    Article Snippet: The sensitivity of TRAIL-resistant cell lines to TRAIL was periodically examined by PI (Sigma, St. Louis, MO) staining and flow cytometry, and never exceeded 5% cell death as described before. .. To identify and isolate CSC, parental cells were collected in Versene solution (Invitrogen, Carlsbad, CA, USA, cat#15040), washed and blocked with flow cytometry buffer (Santa Cruz Biotechnology, Dallas, TX, USA), labeled with the following primary conjugated antibodies and their matched isotypes: anti-CD24-PE-Cy7(561646), anti-EpCAM-FITC (BD Bioscience, San Jose, CA, USA, 347197) anti-CD44-APC-eFluor 780(47044182), anti-CD166-PerCPeFluor 710(46166842), anti Gal3-PE(12-5301-83) (all from - eBioscience, San Diego, CA, USA). .. LIVE/DEAD Fixable Dead Cell Stain (Invitrogen) was used for exclusion of dead cells, and isotype-matched control antibodies were used to set gates for marker positivity in viable single cells.

    Staining:

    Article Title: Uropathogenic Escherichia coli Engages CD14-Dependent Signaling to Enable Bladder-Macrophage-Dependent Control of Acute Urinary Tract Infection.
    Article Snippet: .. Cells were centrifuged and resuspended in 200 μL of wash buffer; cells were counted and placed in flow cytometry tubes, with 106 cells per tube, and stained with anti-mouse F4/80 allophycocyanin-conjugated antibody (eBiosciences) and anti-mouse CD14 primary antibody (Santa Cruz, Dallas, Texas), followed by secondary anti-goat–phycoerythrin antibody (BD Biosciences), for 30 minutes each in the dark (antibodies were used at the concentrations recommended by the manufacturers). .. Cells were washed, resuspended in 4% paraformaldehyde, and analyzed the following day by using a LSRII Fortessa flow cytometer (BD Bioscience, North Ryde, Australia).

    Article Title: CRISPR-Cas9-Mediated Correction of SLC12A3 Gene Mutation Rescues the Gitelman's Disease Phenotype in a Patient-Derived Kidney Organoid System.
    Article Snippet: .. 2023, 24, 3019 11 of 14 serum albumin and 10 mM sodium azide), permeabilized for 30 min using flow cytometry fixation and permeabilization solution (554714; BD Biosciences, San Jose, CA, USA), washed with wash buffer, and stained with stage-specific embryonic antigens SSEA-4 (813-70, 1:100; Santa Cruz Biotechnology, Dallas TX, USA) or TRA-1–81 (TRA-1-81, 1:100; Santa Cruz Biotechnology) surface antibodies for 30 min. Intracellular staining for NANOG (1E6C4, 1:100; Santa Cruz Biotechnology) was performed by sequential incubations with fixation and permeabilization solutions (A and B Fix & Perm Solutions, Invitrogen, BD Bioscience). .. These cells were incubated with FITC-conjugated secondary antibody (BD Bioscience).

    Incubation:

    Article Title: Induction of autophagy, apoptosis and aquisition of resistance in response to piceatannol toxicity in MOLT-4 human leukemia cells.
    Article Snippet: Accepted Manuscript Induction of autophagy, apoptosis and aquisition of resistance in response to piceatannol toxicity in MOLT-4 human leukemia cells Kamila Siedlecka-Kroplewska, Tomasz Ślebioda, Zbigniew Kmieć PII: S0887-2333(19)30002-5 DOI: https://doi.org/10.1016/j.tiv.2019.03.040 Reference: TIV 4508 To appear in: Toxicology in Vitro Received date: 21 January 2019 Revised date: 9 March 2019 Accepted date: 29 March 2019 Please cite this article as: K. Siedlecka-Kroplewska, T. Ślebioda and Z. Kmieć, Induction of autophagy, apoptosis and aquisition of resistance in response to piceatannol toxicity in MOLT-4 human leukemia cells, Toxicology in Vitro, https://doi.org/10.1016/ j.tiv.2019.03.040 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Labeling:

    Article Title: Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics
    Article Snippet: The sensitivity of TRAIL-resistant cell lines to TRAIL was periodically examined by PI (Sigma, St. Louis, MO) staining and flow cytometry, and never exceeded 5% cell death as described before. .. To identify and isolate CSC, parental cells were collected in Versene solution (Invitrogen, Carlsbad, CA, USA, cat#15040), washed and blocked with flow cytometry buffer (Santa Cruz Biotechnology, Dallas, TX, USA), labeled with the following primary conjugated antibodies and their matched isotypes: anti-CD24-PE-Cy7(561646), anti-EpCAM-FITC (BD Bioscience, San Jose, CA, USA, 347197) anti-CD44-APC-eFluor 780(47044182), anti-CD166-PerCPeFluor 710(46166842), anti Gal3-PE(12-5301-83) (all from - eBioscience, San Diego, CA, USA). .. LIVE/DEAD Fixable Dead Cell Stain (Invitrogen) was used for exclusion of dead cells, and isotype-matched control antibodies were used to set gates for marker positivity in viable single cells.



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