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axiovision 4 multichannel fluorescence basic workstation  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axiovision 4 multichannel fluorescence basic workstation
    Axiovision 4 Multichannel Fluorescence Basic Workstation, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visual+basic+script+for+zeiss+axiovision/fluorescence+microscopy+multichannel/pm22492283-35-13-19
    Average 90 stars, based on 1 article reviews
    axiovision 4 multichannel fluorescence basic workstation - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Differential expression of tension-sensitive HOX genes in fibroblasts is associated with different scar types
    Article Snippet: To label nuclei, 4’,6-diamidino-2-phenylindole (DAPI, 1:50000 dilution, Molecular Probes) was treated in the cells for 3min. .. Cells were then imaged using multichannel fluorescence microscopy (Carl Zeiss). .. Cells were cultured on polydimethylsiloxane (PDMS) (Dow Corning, USA), which were pretreated with oxygen plasma (Convance, Femto Science, Korea) for 1 min at a pressure of 0.7 Torr followed by 1μg/cm2 of fibronectin (Invitrogen, USA) coating for 120 min at room temperature.

    Article Title: In vitro cardiomyocyte differentiation of umbilical cord blood cells: crucial role for c-kit(+) cells.
    Article Snippet: Background aims.. Although bone marrow c-kitþ progenitor cells support myocardial regeneration, the cardiomyocyte differentiation potential of umbilical cord blood (UCB) c-kitþ cells is unknown.. Methods.

    Article Title: Physicochemically Tuned Myofibroblasts for Wound Healing Strategy
    Article Snippet: During the staining step, we treat α-smooth muscle actin (α-SMA) antibody (Abcam) (with 1:100 dilution) for 12 hours, Alex Flour 488-fluorescence antibody (Invitrogen) (with 1:200 dilution) for 6 hours, Alex Fluor 568-phalloidin (Invitrogen) (with 1:50 dilution) for 20 min, and 4’,6-diamidino-2-phenylindole (DAPI, Molecular Probe) (with 1:50000 dilution) for 3 min. .. The multichannel fluorescence microscopy (Zeiss) was used for taking immunofluorescence images. ..

    Article Title: Fibrous Matrix Architecture-Dependent Activation of Fibroblasts with a Cancer-Associated Fibroblast-like Phenotype.
    Article Snippet: Cancer-associated fibroblasts (CAFs) are one of the most prevalent cell types within the tumor microenvironment (TME).. While several physicochemical cues from the TME, including growth factors, cytokines, and ECM specificity, have been identified as essential factors for CAF activation, the precise mechanism of how the ECM architecture regulates CAF initiation remains elusive.. Using a gelatin-based electrospun fiber mesh, we examined the effect of matrix fiber density on CAF activation induced by MCF-7 conditioned media (CM).

    Article Title: Tension-sensitive HOX gene expression in fibroblasts for differential scar formation.
    Article Snippet: To label nuclei, 4’,6-diamidino-2-phenylindole (DAPI, 1:50000 dilution, Molecular Probes) was treated in the cells for 3 min. .. Cells were then imaged using multichannel fluorescence microscopy (Carl Zeiss). .. Cells were cultured on polydimethylsiloxane (PDMS) (Dow Corning, USA), which were pretreated with oxygen plasma (Convance, Femto Science, Korea) for 1 min at a pressure of 0.7 Torr followed by 1 μg/cm2 of fibronectin (Invitrogen, USA) coating for 120 min at room temperature.

    Article Title: Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model
    Article Snippet: The secondary antibody for K16 was AF488-conjugated donkey anti-mouse IgG (ThermoFisher); for hBD2 AF546-conjugated donkey anti-goat (ThermoFisher), and for CD3 AF647-conjugated donkey anti-rabbit (ThermoFisher). .. Sections were photographed using a microscope (AxioImager M2; Zeiss, Sliedrecht, the Netherlands) and a high resolution color camera for bright field microscopy (AxioCam 105 color, Zeiss) and a high resolution b/w camera for multichannel fluorescence microscopy (AxioCam 503 Mono). .. Mean epidermal thickness was calculated using AxioVision software version 4.8 (Zeiss) or ZEN blue edition version 2.3 (Zeiss) as epidermal area divided by epidermal surface length.

    Microscopy:

    Article Title: Differential expression of tension-sensitive HOX genes in fibroblasts is associated with different scar types
    Article Snippet: To label nuclei, 4’,6-diamidino-2-phenylindole (DAPI, 1:50000 dilution, Molecular Probes) was treated in the cells for 3min. .. Cells were then imaged using multichannel fluorescence microscopy (Carl Zeiss). .. Cells were cultured on polydimethylsiloxane (PDMS) (Dow Corning, USA), which were pretreated with oxygen plasma (Convance, Femto Science, Korea) for 1 min at a pressure of 0.7 Torr followed by 1μg/cm2 of fibronectin (Invitrogen, USA) coating for 120 min at room temperature.

    Article Title: Physicochemically Tuned Myofibroblasts for Wound Healing Strategy
    Article Snippet: During the staining step, we treat α-smooth muscle actin (α-SMA) antibody (Abcam) (with 1:100 dilution) for 12 hours, Alex Flour 488-fluorescence antibody (Invitrogen) (with 1:200 dilution) for 6 hours, Alex Fluor 568-phalloidin (Invitrogen) (with 1:50 dilution) for 20 min, and 4’,6-diamidino-2-phenylindole (DAPI, Molecular Probe) (with 1:50000 dilution) for 3 min. .. The multichannel fluorescence microscopy (Zeiss) was used for taking immunofluorescence images. ..

    Article Title: Fibrous Matrix Architecture-Dependent Activation of Fibroblasts with a Cancer-Associated Fibroblast-like Phenotype.
    Article Snippet: Cancer-associated fibroblasts (CAFs) are one of the most prevalent cell types within the tumor microenvironment (TME).. While several physicochemical cues from the TME, including growth factors, cytokines, and ECM specificity, have been identified as essential factors for CAF activation, the precise mechanism of how the ECM architecture regulates CAF initiation remains elusive.. Using a gelatin-based electrospun fiber mesh, we examined the effect of matrix fiber density on CAF activation induced by MCF-7 conditioned media (CM).

    Article Title: Tension-sensitive HOX gene expression in fibroblasts for differential scar formation.
    Article Snippet: To label nuclei, 4’,6-diamidino-2-phenylindole (DAPI, 1:50000 dilution, Molecular Probes) was treated in the cells for 3 min. .. Cells were then imaged using multichannel fluorescence microscopy (Carl Zeiss). .. Cells were cultured on polydimethylsiloxane (PDMS) (Dow Corning, USA), which were pretreated with oxygen plasma (Convance, Femto Science, Korea) for 1 min at a pressure of 0.7 Torr followed by 1 μg/cm2 of fibronectin (Invitrogen, USA) coating for 120 min at room temperature.

    Article Title: Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model
    Article Snippet: The secondary antibody for K16 was AF488-conjugated donkey anti-mouse IgG (ThermoFisher); for hBD2 AF546-conjugated donkey anti-goat (ThermoFisher), and for CD3 AF647-conjugated donkey anti-rabbit (ThermoFisher). .. Sections were photographed using a microscope (AxioImager M2; Zeiss, Sliedrecht, the Netherlands) and a high resolution color camera for bright field microscopy (AxioCam 105 color, Zeiss) and a high resolution b/w camera for multichannel fluorescence microscopy (AxioCam 503 Mono). .. Mean epidermal thickness was calculated using AxioVision software version 4.8 (Zeiss) or ZEN blue edition version 2.3 (Zeiss) as epidermal area divided by epidermal surface length.

    Immunofluorescence:

    Article Title: Physicochemically Tuned Myofibroblasts for Wound Healing Strategy
    Article Snippet: During the staining step, we treat α-smooth muscle actin (α-SMA) antibody (Abcam) (with 1:100 dilution) for 12 hours, Alex Flour 488-fluorescence antibody (Invitrogen) (with 1:200 dilution) for 6 hours, Alex Fluor 568-phalloidin (Invitrogen) (with 1:50 dilution) for 20 min, and 4’,6-diamidino-2-phenylindole (DAPI, Molecular Probe) (with 1:50000 dilution) for 3 min. .. The multichannel fluorescence microscopy (Zeiss) was used for taking immunofluorescence images. ..

    Staining:

    Article Title: Fibrous Matrix Architecture-Dependent Activation of Fibroblasts with a Cancer-Associated Fibroblast-like Phenotype.
    Article Snippet: Cancer-associated fibroblasts (CAFs) are one of the most prevalent cell types within the tumor microenvironment (TME).. While several physicochemical cues from the TME, including growth factors, cytokines, and ECM specificity, have been identified as essential factors for CAF activation, the precise mechanism of how the ECM architecture regulates CAF initiation remains elusive.. Using a gelatin-based electrospun fiber mesh, we examined the effect of matrix fiber density on CAF activation induced by MCF-7 conditioned media (CM).

    other:

    Article Title: The claustrum is critical for maintaining working memory information
    Article Snippet: The samples were imaged using a ZEISS Axiocam multi-channel fluorescence imaging microscope using either a 4X or 10X objective.

    Software:

    Article Title: Parkin Promotes Mitophagic Cell Death in Adult Hippocampal Neural Stem Cells Following Insulin Withdrawal.
    Article Snippet: .. Images were obtained by AxioVision 4 module Multichannel software (Carl Zeiss) and were analyzed by Pixcavator student edition analysis software (Intelligent Perception Co.). .. To assess cell death, cells were stained with Hoechst 33342 (Invitrogen) and propidium iodide (PI) (Sigma-Aldrich), and were imaged under a fluorescence microscope (Axiovert 40 CFL, Carl Zeiss, Oberkochen, Germany).



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    Carl Zeiss visual basic script for zeiss axiovision
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