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visium cytassist spatial gene expression reagent kits  (Spatial Transcriptomics Inc)

 
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    Spatial Transcriptomics Inc visium cytassist spatial gene expression reagent kits
    Visium Cytassist Spatial Gene Expression Reagent Kits, supplied by Spatial Transcriptomics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+tissue+optimization+kit/cytassist+expression+gene+kits+reagent+spatial+visium/pmc12552549-511-5-0
    Average 86 stars, based on 1 article reviews
    visium cytassist spatial gene expression reagent kits - by Bioz Stars, 2026-10
    86/100 stars

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    other:


    Spatial Transcriptomics:

    Article Title: Neutrophil Extracellular Trap Reprograms Cancer Metabolism to Form a Metastatic Niche Promoting Non-Small Cell Lung Cancer Brain Metastasis.
    Article Snippet: .. 10× Visium CytAssist Spatial Transcriptomics Sequencing and Data PreProcessing: Freshly collected tumor tissues were divided into appropriate sizes (6.5 mm × 6.5 mm) and embedded in optimal cutting temperature compound and quickly frozen on dry ice. ..

    Article Title: Mapping the secondary response to traumatic brain injury using spatial transcriptomics shows acute 4-aminopyridine treatment mitigates axonal and molecular pathology.
    Article Snippet: Sections from each mouse had RNA quality confirmed as DV200 greater than 50%, measured using the Fragment Analyzer 5200 (Agilent; Cat#M5310AA) and DNF-472 High Sensitivity RNA Kit (Agilent, Cat#DNF-472-FR). .. Coronal FFPE brain tissue sections (7 μm) were prepared for Visium CytAssist spatial transcriptomics prepared into cDNA libraries and sequenced using Illumina NovaSeq sequencers (further details in Supplemental Information). .. FFPE Immunohistochemistry: Coronal sections (7 μm) anterior to the spatial transcriptomics sections and under the TBI impact site were immunolabeled for myelin basic protein (MBP), β−APP, GFAP, or IBA1 (Table S2).

    Sequencing:

    Article Title: Neutrophil Extracellular Trap Reprograms Cancer Metabolism to Form a Metastatic Niche Promoting Non-Small Cell Lung Cancer Brain Metastasis.
    Article Snippet: .. 10× Visium CytAssist Spatial Transcriptomics Sequencing and Data PreProcessing: Freshly collected tumor tissues were divided into appropriate sizes (6.5 mm × 6.5 mm) and embedded in optimal cutting temperature compound and quickly frozen on dry ice. ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Mapping the secondary response to traumatic brain injury using spatial transcriptomics shows acute 4-aminopyridine treatment mitigates axonal and molecular pathology.
    Article Snippet: Sections from each mouse had RNA quality confirmed as DV200 greater than 50%, measured using the Fragment Analyzer 5200 (Agilent; Cat#M5310AA) and DNF-472 High Sensitivity RNA Kit (Agilent, Cat#DNF-472-FR). .. Coronal FFPE brain tissue sections (7 μm) were prepared for Visium CytAssist spatial transcriptomics prepared into cDNA libraries and sequenced using Illumina NovaSeq sequencers (further details in Supplemental Information). .. FFPE Immunohistochemistry: Coronal sections (7 μm) anterior to the spatial transcriptomics sections and under the TBI impact site were immunolabeled for myelin basic protein (MBP), β−APP, GFAP, or IBA1 (Table S2).



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    ( A ) Comparison of SERPINE1 expression in SSc and healthy control skin biopsy samples from the GEO dataset ( GSE58095 ). ( B ) Correlation analysis of modified Rodnan skin scores (mRSS) versus SERPINE1 expression from the data in A . ( C ) Enriched signature for expression of extracellular matrix (ECM) genes along with expression of the myofibroblast marker COL8A1 , and SERPINE1 (PAI-1) expression in SSc skin on the 10x Visium spatial platform (data representative of n = 4). ( D ) Violin plots of SERPINE1 expression in controls versus SSc skin across single-cell RNA-seq data from different cellular populations (top) and fibroblast subsets (bottom) ( n = 18 healthy controls, n = 22 SSc patients). ( E ) Enriched biological processes in SERPINE1 + versus SERPINE1 – COL8A1 myofibroblasts. ( F ) IHC of PAI-1 in SSc skin biopsy (data representative of n = 6). ( G ) IHC of PAI-1 in skin biopsy from a healthy volunteer. Scale bars: 500 μm on overall biopsy, 50 μm on the insets. **** P < 0.0001 by 2-tailed Mann-Whitney test in A . P = 0.0003 by Spearman’s r correlation test in B .

    Journal: JCI Insight

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    doi: 10.1172/jci.insight.195005

    Figure Lengend Snippet: ( A ) Comparison of SERPINE1 expression in SSc and healthy control skin biopsy samples from the GEO dataset ( GSE58095 ). ( B ) Correlation analysis of modified Rodnan skin scores (mRSS) versus SERPINE1 expression from the data in A . ( C ) Enriched signature for expression of extracellular matrix (ECM) genes along with expression of the myofibroblast marker COL8A1 , and SERPINE1 (PAI-1) expression in SSc skin on the 10x Visium spatial platform (data representative of n = 4). ( D ) Violin plots of SERPINE1 expression in controls versus SSc skin across single-cell RNA-seq data from different cellular populations (top) and fibroblast subsets (bottom) ( n = 18 healthy controls, n = 22 SSc patients). ( E ) Enriched biological processes in SERPINE1 + versus SERPINE1 – COL8A1 myofibroblasts. ( F ) IHC of PAI-1 in SSc skin biopsy (data representative of n = 6). ( G ) IHC of PAI-1 in skin biopsy from a healthy volunteer. Scale bars: 500 μm on overall biopsy, 50 μm on the insets. **** P < 0.0001 by 2-tailed Mann-Whitney test in A . P = 0.0003 by Spearman’s r correlation test in B .

    Article Snippet: Optimization of tissue permeabilization was performed on 20 μm sections using a Visium Spatial Tissue Optimization Reagents Kit (10x Genomics), which established an optimal permeabilization time of 9 minutes.

    Techniques: Comparison, Expressing, Control, Modification, Marker, Single Cell, RNA Sequencing, MANN-WHITNEY