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10X Genomics visium reagent kits
Visium Reagent Kits, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/visium+spatial+tissue+optimization+kit/kits+reagent/pmc10456192-76-9-0
Average 86 stars, based on 1 article reviews
visium reagent kits - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

cDNA Library Assay:

Article Title: Pleiotropic germline PTEN mutations influence gastrulation through dysregulated AKT activation.
Article Snippet: .. Sample processing with 10X genomics and cDNA library preparation Using 10X Genomics Chromium Single Cell 3’ Reagent Kits (version 3.1), 6,000 to 10,000 cells per sample were captured for library and sequencing preparation. .. Following the quality confirmation of the single cell suspension, the single cells, the gel beads, and the emulsion oil were added to the 10X Genomics Single Cell Chip G. Immediately following the droplet generation, samples were transferred to a PCR 8-tube strip (USA Scientific) for reverse transcription reaction using SimpliAmp thermal cycler (Applied Biosystems). cDNA generated by reverse transcription was recovered using the recovery reagent provided by 10X Genomics.

Single Cell:

Article Title: Pleiotropic germline PTEN mutations influence gastrulation through dysregulated AKT activation.
Article Snippet: .. Sample processing with 10X genomics and cDNA library preparation Using 10X Genomics Chromium Single Cell 3’ Reagent Kits (version 3.1), 6,000 to 10,000 cells per sample were captured for library and sequencing preparation. .. Following the quality confirmation of the single cell suspension, the single cells, the gel beads, and the emulsion oil were added to the 10X Genomics Single Cell Chip G. Immediately following the droplet generation, samples were transferred to a PCR 8-tube strip (USA Scientific) for reverse transcription reaction using SimpliAmp thermal cycler (Applied Biosystems). cDNA generated by reverse transcription was recovered using the recovery reagent provided by 10X Genomics.

Article Title: Lymphatic egress recycles tumor-experienced effector CD8 T cells to sustain immune surveillance
Article Snippet: .. Libraries were prepared using Single Cell 3’ Reagent kits (10x Genomics) and sequenced using Illumina Novaseq 6000. .. Single-cell RNA-seq data were processed with Cell Ranger (10x Genomics) using the mouse reference genome, and downstream analyses were performed in R using the Seurat package.

Article Title: Systemic immune profiling uncovers divergent mechanisms and predictive biomarkers of response to combination immunotherapies in hepatocellular carcinoma.
Article Snippet: Approximately 10 million PBMCs were resuspended in 45 μL of Cell Staining Buffer (Cat. 420201, BioLegend, USA), incubated with 5 μL of Human TruStain FcX (Cat. 422301) at 4°C for 10 min, followed by staining with DNAbarcoded antibodies at 4°C for 30 min. .. Cells were washed once with cold PBS containing 0.04% BSA and three times with PBS containing 1% BSA, then resuspended at 1000 cells/μL in PBS with 0.04% BSA. scRNA-seq (CITE-seq) Single- cell suspensions were processed using the Chromium Single Cell 5’ Reagent Kits (10x Genomics) according to the manufacturer’s protocol (CG000149 Rev D). ..

Article Title: TLR3 Expression in Villus-Like Enterocytes Drives IFN-III Responses to Enteroviruses
Article Snippet: .. Libraries were prepared from ∼10,000 cells using 10x Genomics Single Cell 3’ reagent kits on a Chromium instrument (10x Genomics) by the Molecular Genomics Core at the Duke Molecular Physiology Institute. .. Sequencing was performed on a NovaSeq 6000 sequencer (Illumina) using a S2 flow cell which was predicted to yield around 62k reads/cell.

Article Title: Single-cell and deep learning identify hypoxia-responsive lncRNAs predicting outcomes in colorectal cancer.
Article Snippet: .. Libraries were generated using Chromium Single Cell 5'v2 and V(D)J Reagent kits (10x Genomics), following the manufacturer’s instructions, with unique sample indices for each library. .. Quantification was conducted using a High Sensitivity DNA Chip (Agilent) on a Bioanalyzer 2100 and a Qubit High Sensitivity DNA Assay (Thermo Fisher Scientific).

Article Title: Transcriptional regulators predicted to drive macrophage dysregulation during impaired wound healing in diabetic mice
Article Snippet: .. Sorted live CD11b + CD45 + Ly6G − cells were processed for scRNA-seq using the 10x Chromium Next GEM Single Cell 3’ Reagent Kits (10X Genomics, San Francisco, CA, USA). .. Libraries were sequenced on HiSeq Sequencing Systems (Illumina, San Diego, CA, USA) with paired-end reads aiming for 100,000 reads/cell [ ].

Article Title: STING mediates lysosomal quality control and recovery through its proton channel function and TFEB activation in lysosomal storage disorders
Article Snippet: .. Single-nuclei RNA-seq libraries were prepared using the Chromium Single Cell 5’ Reagent Kits (10x Genomics) according to the manufacturer’s protocol. .. Libraries were sequenced using an Illumina NovaSeq 6000.

Sequencing:

Article Title: Pleiotropic germline PTEN mutations influence gastrulation through dysregulated AKT activation.
Article Snippet: .. Sample processing with 10X genomics and cDNA library preparation Using 10X Genomics Chromium Single Cell 3’ Reagent Kits (version 3.1), 6,000 to 10,000 cells per sample were captured for library and sequencing preparation. .. Following the quality confirmation of the single cell suspension, the single cells, the gel beads, and the emulsion oil were added to the 10X Genomics Single Cell Chip G. Immediately following the droplet generation, samples were transferred to a PCR 8-tube strip (USA Scientific) for reverse transcription reaction using SimpliAmp thermal cycler (Applied Biosystems). cDNA generated by reverse transcription was recovered using the recovery reagent provided by 10X Genomics.

Generated:

Article Title: Single-cell and deep learning identify hypoxia-responsive lncRNAs predicting outcomes in colorectal cancer.
Article Snippet: .. Libraries were generated using Chromium Single Cell 5'v2 and V(D)J Reagent kits (10x Genomics), following the manufacturer’s instructions, with unique sample indices for each library. .. Quantification was conducted using a High Sensitivity DNA Chip (Agilent) on a Bioanalyzer 2100 and a Qubit High Sensitivity DNA Assay (Thermo Fisher Scientific).

other:

Article Title: Oligodendrocyte dysfunction contributes to motor deficits and Purkinje cell axonopathy in spinocerebellar ataxia type 1.
Article Snippet: The purified nuclei were resuspended in 1 mL of NSB, filtered through a 40 μm cell strainer 528 21 (Fisher Scientific; 22-363-547), and quantified using a Countess III FL Cell Counter (Thermo Fisher 529 Scientific; AMQAF2000) with trypan blue.

RNA Sequencing:

Article Title: STING mediates lysosomal quality control and recovery through its proton channel function and TFEB activation in lysosomal storage disorders
Article Snippet: .. Single-nuclei RNA-seq libraries were prepared using the Chromium Single Cell 5’ Reagent Kits (10x Genomics) according to the manufacturer’s protocol. .. Libraries were sequenced using an Illumina NovaSeq 6000.



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( A ) Comparison of SERPINE1 expression in SSc and healthy control skin biopsy samples from the GEO dataset ( GSE58095 ). ( B ) Correlation analysis of modified Rodnan skin scores (mRSS) versus SERPINE1 expression from the data in A . ( C ) Enriched signature for expression of extracellular matrix (ECM) genes along with expression of the myofibroblast marker COL8A1 , and SERPINE1 (PAI-1) expression in SSc skin on <t>the</t> <t>10x</t> <t>Visium</t> spatial platform (data representative of n = 4). ( D ) Violin plots of SERPINE1 expression in controls versus SSc skin across single-cell RNA-seq data from different cellular populations (top) and fibroblast subsets (bottom) ( n = 18 healthy controls, n = 22 SSc patients). ( E ) Enriched biological processes in SERPINE1 + versus SERPINE1 – COL8A1 myofibroblasts. ( F ) IHC of PAI-1 in SSc skin biopsy (data representative of n = 6). ( G ) IHC of PAI-1 in skin biopsy from a healthy volunteer. Scale bars: 500 μm on overall biopsy, 50 μm on the insets. **** P < 0.0001 by 2-tailed Mann-Whitney test in A . P = 0.0003 by Spearman’s r correlation test in B .
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( A ) Comparison of SERPINE1 expression in SSc and healthy control skin biopsy samples from the GEO dataset ( GSE58095 ). ( B ) Correlation analysis of modified Rodnan skin scores (mRSS) versus SERPINE1 expression from the data in A . ( C ) Enriched signature for expression of extracellular matrix (ECM) genes along with expression of the myofibroblast marker COL8A1 , and SERPINE1 (PAI-1) expression in SSc skin on <t>the</t> <t>10x</t> <t>Visium</t> spatial platform (data representative of n = 4). ( D ) Violin plots of SERPINE1 expression in controls versus SSc skin across single-cell RNA-seq data from different cellular populations (top) and fibroblast subsets (bottom) ( n = 18 healthy controls, n = 22 SSc patients). ( E ) Enriched biological processes in SERPINE1 + versus SERPINE1 – COL8A1 myofibroblasts. ( F ) IHC of PAI-1 in SSc skin biopsy (data representative of n = 6). ( G ) IHC of PAI-1 in skin biopsy from a healthy volunteer. Scale bars: 500 μm on overall biopsy, 50 μm on the insets. **** P < 0.0001 by 2-tailed Mann-Whitney test in A . P = 0.0003 by Spearman’s r correlation test in B .
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( A ) Comparison of SERPINE1 expression in SSc and healthy control skin biopsy samples from the GEO dataset ( GSE58095 ). ( B ) Correlation analysis of modified Rodnan skin scores (mRSS) versus SERPINE1 expression from the data in A . ( C ) Enriched signature for expression of extracellular matrix (ECM) genes along with expression of the myofibroblast marker COL8A1 , and SERPINE1 (PAI-1) expression in SSc skin on <t>the</t> <t>10x</t> <t>Visium</t> spatial platform (data representative of n = 4). ( D ) Violin plots of SERPINE1 expression in controls versus SSc skin across single-cell RNA-seq data from different cellular populations (top) and fibroblast subsets (bottom) ( n = 18 healthy controls, n = 22 SSc patients). ( E ) Enriched biological processes in SERPINE1 + versus SERPINE1 – COL8A1 myofibroblasts. ( F ) IHC of PAI-1 in SSc skin biopsy (data representative of n = 6). ( G ) IHC of PAI-1 in skin biopsy from a healthy volunteer. Scale bars: 500 μm on overall biopsy, 50 μm on the insets. **** P < 0.0001 by 2-tailed Mann-Whitney test in A . P = 0.0003 by Spearman’s r correlation test in B .
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( A ) Comparison of SERPINE1 expression in SSc and healthy control skin biopsy samples from the GEO dataset ( GSE58095 ). ( B ) Correlation analysis of modified Rodnan skin scores (mRSS) versus SERPINE1 expression from the data in A . ( C ) Enriched signature for expression of extracellular matrix (ECM) genes along with expression of the myofibroblast marker COL8A1 , and SERPINE1 (PAI-1) expression in SSc skin on <t>the</t> <t>10x</t> <t>Visium</t> spatial platform (data representative of n = 4). ( D ) Violin plots of SERPINE1 expression in controls versus SSc skin across single-cell RNA-seq data from different cellular populations (top) and fibroblast subsets (bottom) ( n = 18 healthy controls, n = 22 SSc patients). ( E ) Enriched biological processes in SERPINE1 + versus SERPINE1 – COL8A1 myofibroblasts. ( F ) IHC of PAI-1 in SSc skin biopsy (data representative of n = 6). ( G ) IHC of PAI-1 in skin biopsy from a healthy volunteer. Scale bars: 500 μm on overall biopsy, 50 μm on the insets. **** P < 0.0001 by 2-tailed Mann-Whitney test in A . P = 0.0003 by Spearman’s r correlation test in B .
Visium Spatial Tissue Optimization Reagent Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
visium spatial tissue optimization reagent kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results


( A ) Comparison of SERPINE1 expression in SSc and healthy control skin biopsy samples from the GEO dataset ( GSE58095 ). ( B ) Correlation analysis of modified Rodnan skin scores (mRSS) versus SERPINE1 expression from the data in A . ( C ) Enriched signature for expression of extracellular matrix (ECM) genes along with expression of the myofibroblast marker COL8A1 , and SERPINE1 (PAI-1) expression in SSc skin on the 10x Visium spatial platform (data representative of n = 4). ( D ) Violin plots of SERPINE1 expression in controls versus SSc skin across single-cell RNA-seq data from different cellular populations (top) and fibroblast subsets (bottom) ( n = 18 healthy controls, n = 22 SSc patients). ( E ) Enriched biological processes in SERPINE1 + versus SERPINE1 – COL8A1 myofibroblasts. ( F ) IHC of PAI-1 in SSc skin biopsy (data representative of n = 6). ( G ) IHC of PAI-1 in skin biopsy from a healthy volunteer. Scale bars: 500 μm on overall biopsy, 50 μm on the insets. **** P < 0.0001 by 2-tailed Mann-Whitney test in A . P = 0.0003 by Spearman’s r correlation test in B .

Journal: JCI Insight

Article Title: A potent inhibitor of PAI-1, MDI-2517, mitigates disease severity in a preclinical systemic sclerosis model

doi: 10.1172/jci.insight.195005

Figure Lengend Snippet: ( A ) Comparison of SERPINE1 expression in SSc and healthy control skin biopsy samples from the GEO dataset ( GSE58095 ). ( B ) Correlation analysis of modified Rodnan skin scores (mRSS) versus SERPINE1 expression from the data in A . ( C ) Enriched signature for expression of extracellular matrix (ECM) genes along with expression of the myofibroblast marker COL8A1 , and SERPINE1 (PAI-1) expression in SSc skin on the 10x Visium spatial platform (data representative of n = 4). ( D ) Violin plots of SERPINE1 expression in controls versus SSc skin across single-cell RNA-seq data from different cellular populations (top) and fibroblast subsets (bottom) ( n = 18 healthy controls, n = 22 SSc patients). ( E ) Enriched biological processes in SERPINE1 + versus SERPINE1 – COL8A1 myofibroblasts. ( F ) IHC of PAI-1 in SSc skin biopsy (data representative of n = 6). ( G ) IHC of PAI-1 in skin biopsy from a healthy volunteer. Scale bars: 500 μm on overall biopsy, 50 μm on the insets. **** P < 0.0001 by 2-tailed Mann-Whitney test in A . P = 0.0003 by Spearman’s r correlation test in B .

Article Snippet: Optimization of tissue permeabilization was performed on 20 μm sections using a Visium Spatial Tissue Optimization Reagents Kit (10x Genomics), which established an optimal permeabilization time of 9 minutes.

Techniques: Comparison, Expressing, Control, Modification, Marker, Single Cell, RNA Sequencing, MANN-WHITNEY