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permeabilization enzyme provided by 10x genomics in the visium gene expression slide reagent kit pn 1000184  (10X Genomics)

 
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    10X Genomics permeabilization enzyme provided by 10x genomics in the visium gene expression slide reagent kit pn 1000184
    Permeabilization Enzyme Provided By 10x Genomics In The Visium Gene Expression Slide Reagent Kit Pn 1000184, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+tissue+optimization+kit/enzyme+permeabilization/pmc09882298-153-5-9
    Average 86 stars, based on 1 article reviews
    permeabilization enzyme provided by 10x genomics in the visium gene expression slide reagent kit pn 1000184 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Spatial transcriptomics of the aging mouse brain reveals origins of inflammation in the white matter.
    Article Snippet: The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. .. The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. ..

    Article Title: Spatial transcriptomics of the aging mouse brain reveals origins of inflammation in the white matter
    Article Snippet: To optimize permeabilization time for mouse brain tissue, the Visium spatial tissue optimization slide was placed in the slide cassette (10X Genomics, PN3000406). .. The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3 min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. ..

    Gene Expression:

    Article Title: Spatial transcriptomics of the aging mouse brain reveals origins of inflammation in the white matter.
    Article Snippet: The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. .. The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. ..

    Article Title: Spatial transcriptomics of the aging mouse brain reveals origins of inflammation in the white matter
    Article Snippet: To optimize permeabilization time for mouse brain tissue, the Visium spatial tissue optimization slide was placed in the slide cassette (10X Genomics, PN3000406). .. The tissue sections on the slide were incubated with the permeabilization enzyme (10X Genomics, PN2000214) for a time course of 30, 24, 18, 12, 6, and 3 min to identify an optimal tissue permeabilization time for further use in the visium spatial gene expression. ..

    Article Title: Spatial transcriptomics reveals unique gene expression changes in different brain regions after sleep deprivation.
    Article Snippet: Raw images were stitched together with the CellSens software (Version 3.2; Olympus) and exported as tiff files. .. Tissue was then permeabilized with Permeabilization Enzyme (provided by 10X Genomics in the Visium Gene Expression Slide & Reagent Kit, PN1000184) for 18min as determined based on tissue optimization timecourse experiments. ..

    Imaging:

    Article Title: Spatio-temporal transcriptomics of chromothriptic SHH-medulloblastoma identifies multiple genetic clones that resist treatment and drive relapse
    Article Snippet: .. Permeabilization was performed on a pre-heated thermal cycler at 37 °C with Permeabilization Enzyme for 12 min. Permeabilization time was determined in the previous Tissue Optimization Experiment following the Visium Spatial User Guide CG000238_VisiumSpatialTissueOptimizationUserGuide_RevC using the Visium Spatial Tissue Optimization Slide & Reagents Kit (#1000193, 10x Genomics), by permeabilizing tissue for different times, synthesising fluorescent cDNA, imaging, and determining the optimal signal. ..

    Saline:

    Article Title: Spatial and single-cell transcriptomics decipher the cellular environment containing HLA-G+ cancer cells and SPP1+ macrophages in colorectal cancer.
    Article Snippet: .. To optimize the permeabilization time of the sections, they were treated with the permeabilization enzyme (10x Genomics: 2000214) for 60 min, washed in 0.13 saline sodium citrate (SSC) buffer (Sigma-Aldrich), and imaged. .. Tissues were permeabilized with the permeabilization enzyme (10x Genomics: 2000214) for 50 min, washed with 0.13 SSC buffer (Sigma-Aldrich), and imaged.



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    Image Search Results


    ( A ) Comparison of SERPINE1 expression in SSc and healthy control skin biopsy samples from the GEO dataset ( GSE58095 ). ( B ) Correlation analysis of modified Rodnan skin scores (mRSS) versus SERPINE1 expression from the data in A . ( C ) Enriched signature for expression of extracellular matrix (ECM) genes along with expression of the myofibroblast marker COL8A1 , and SERPINE1 (PAI-1) expression in SSc skin on the 10x Visium spatial platform (data representative of n = 4). ( D ) Violin plots of SERPINE1 expression in controls versus SSc skin across single-cell RNA-seq data from different cellular populations (top) and fibroblast subsets (bottom) ( n = 18 healthy controls, n = 22 SSc patients). ( E ) Enriched biological processes in SERPINE1 + versus SERPINE1 – COL8A1 myofibroblasts. ( F ) IHC of PAI-1 in SSc skin biopsy (data representative of n = 6). ( G ) IHC of PAI-1 in skin biopsy from a healthy volunteer. Scale bars: 500 μm on overall biopsy, 50 μm on the insets. **** P < 0.0001 by 2-tailed Mann-Whitney test in A . P = 0.0003 by Spearman’s r correlation test in B .

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    doi: 10.1172/jci.insight.195005

    Figure Lengend Snippet: ( A ) Comparison of SERPINE1 expression in SSc and healthy control skin biopsy samples from the GEO dataset ( GSE58095 ). ( B ) Correlation analysis of modified Rodnan skin scores (mRSS) versus SERPINE1 expression from the data in A . ( C ) Enriched signature for expression of extracellular matrix (ECM) genes along with expression of the myofibroblast marker COL8A1 , and SERPINE1 (PAI-1) expression in SSc skin on the 10x Visium spatial platform (data representative of n = 4). ( D ) Violin plots of SERPINE1 expression in controls versus SSc skin across single-cell RNA-seq data from different cellular populations (top) and fibroblast subsets (bottom) ( n = 18 healthy controls, n = 22 SSc patients). ( E ) Enriched biological processes in SERPINE1 + versus SERPINE1 – COL8A1 myofibroblasts. ( F ) IHC of PAI-1 in SSc skin biopsy (data representative of n = 6). ( G ) IHC of PAI-1 in skin biopsy from a healthy volunteer. Scale bars: 500 μm on overall biopsy, 50 μm on the insets. **** P < 0.0001 by 2-tailed Mann-Whitney test in A . P = 0.0003 by Spearman’s r correlation test in B .

    Article Snippet: Optimization of tissue permeabilization was performed on 20 μm sections using a Visium Spatial Tissue Optimization Reagents Kit (10x Genomics), which established an optimal permeabilization time of 9 minutes.

    Techniques: Comparison, Expressing, Control, Modification, Marker, Single Cell, RNA Sequencing, MANN-WHITNEY