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mettl3 coding sequence  (Sangon Biotech)

 
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    Structured Review

    Sangon Biotech mettl3 coding sequence
    ( A) Details of seven potential methylation modification sites on circSIK2. (B ) The relative expression of circSIK2, <t>METTL3,</t> and SIK2 mRNA in myoblast cells after transfected with pCD25-circSIK2 + pCD3.1, pCD25-circSIK2 + pCD3.1-METTL3, and pCD25 + pCD3.1. ( C) Upper: the expression of SIK2-176aa-flag after transfected with pCD3.1-SIK2-176aa-flag and pCD3.1-SIK2-176aa-flag + pCD3.1-METTL3. Lower: the expression of SIK2-176aa after transfected with pCD3.1-SIK2-176aa-flag, pCD25-circSIK2, and pCD25-circSIK2 + pCD3.1-METTL3. (D ) Dual-luciferase reporter assay using the different regions of the circSIK2 after co-transfected with METTL3 in DF-1 cells. ( E) Upper: Dual-luciferase reporter assay using the 151–200 fragment after co-transfected with METTL3 in DF-1 cells. Under: Dual-luciferase reporter assay after 131A-WT and 131A-MT fragment was co-transfected with METTL14 in DF-1 cells. (F ) Amplification curve and qPCR CT value in circSIK2 131 m 6 A site and 125 A site after METTL3 overexpression in myoblast cells. ( G, H) circSIK2 pulldown image ( G ) (OV = pCD25-circSIK2, NC = pCD25) and binding proteins’ string network ( H ) screened by mass spectrometry in myoblast cells. ( I ) circSIK2 pulldown results confirmed by western blot with HNRNPA2B1 antibody in myoblast cells. (J) The expression pattern of METTL3, METTL14, EIF3A, and EIF3F in different stages of myoblast differentiation. GM (growth media; n = 3) stands for myoblasts in the proliferative phase. DM (differentiation media; n = 3), DM1-DM6 means differentiation from day 1 to day 6.
    Mettl3 Coding Sequence, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/viral+reference+sequence+refseq+dataset/flag+overexpression+plasmid+ypel3/pmc12578262-302-5-17
    Average 86 stars, based on 1 article reviews
    mettl3 coding sequence - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "METTL3 facilitates the translation of CircSIK2 during chicken myogenesis in an m 6 A dependent manner"

    Article Title: METTL3 facilitates the translation of CircSIK2 during chicken myogenesis in an m 6 A dependent manner

    Journal: PLOS Genetics

    doi: 10.1371/journal.pgen.1011934

    ( A) Details of seven potential methylation modification sites on circSIK2. (B ) The relative expression of circSIK2, METTL3, and SIK2 mRNA in myoblast cells after transfected with pCD25-circSIK2 + pCD3.1, pCD25-circSIK2 + pCD3.1-METTL3, and pCD25 + pCD3.1. ( C) Upper: the expression of SIK2-176aa-flag after transfected with pCD3.1-SIK2-176aa-flag and pCD3.1-SIK2-176aa-flag + pCD3.1-METTL3. Lower: the expression of SIK2-176aa after transfected with pCD3.1-SIK2-176aa-flag, pCD25-circSIK2, and pCD25-circSIK2 + pCD3.1-METTL3. (D ) Dual-luciferase reporter assay using the different regions of the circSIK2 after co-transfected with METTL3 in DF-1 cells. ( E) Upper: Dual-luciferase reporter assay using the 151–200 fragment after co-transfected with METTL3 in DF-1 cells. Under: Dual-luciferase reporter assay after 131A-WT and 131A-MT fragment was co-transfected with METTL14 in DF-1 cells. (F ) Amplification curve and qPCR CT value in circSIK2 131 m 6 A site and 125 A site after METTL3 overexpression in myoblast cells. ( G, H) circSIK2 pulldown image ( G ) (OV = pCD25-circSIK2, NC = pCD25) and binding proteins’ string network ( H ) screened by mass spectrometry in myoblast cells. ( I ) circSIK2 pulldown results confirmed by western blot with HNRNPA2B1 antibody in myoblast cells. (J) The expression pattern of METTL3, METTL14, EIF3A, and EIF3F in different stages of myoblast differentiation. GM (growth media; n = 3) stands for myoblasts in the proliferative phase. DM (differentiation media; n = 3), DM1-DM6 means differentiation from day 1 to day 6.
    Figure Legend Snippet: ( A) Details of seven potential methylation modification sites on circSIK2. (B ) The relative expression of circSIK2, METTL3, and SIK2 mRNA in myoblast cells after transfected with pCD25-circSIK2 + pCD3.1, pCD25-circSIK2 + pCD3.1-METTL3, and pCD25 + pCD3.1. ( C) Upper: the expression of SIK2-176aa-flag after transfected with pCD3.1-SIK2-176aa-flag and pCD3.1-SIK2-176aa-flag + pCD3.1-METTL3. Lower: the expression of SIK2-176aa after transfected with pCD3.1-SIK2-176aa-flag, pCD25-circSIK2, and pCD25-circSIK2 + pCD3.1-METTL3. (D ) Dual-luciferase reporter assay using the different regions of the circSIK2 after co-transfected with METTL3 in DF-1 cells. ( E) Upper: Dual-luciferase reporter assay using the 151–200 fragment after co-transfected with METTL3 in DF-1 cells. Under: Dual-luciferase reporter assay after 131A-WT and 131A-MT fragment was co-transfected with METTL14 in DF-1 cells. (F ) Amplification curve and qPCR CT value in circSIK2 131 m 6 A site and 125 A site after METTL3 overexpression in myoblast cells. ( G, H) circSIK2 pulldown image ( G ) (OV = pCD25-circSIK2, NC = pCD25) and binding proteins’ string network ( H ) screened by mass spectrometry in myoblast cells. ( I ) circSIK2 pulldown results confirmed by western blot with HNRNPA2B1 antibody in myoblast cells. (J) The expression pattern of METTL3, METTL14, EIF3A, and EIF3F in different stages of myoblast differentiation. GM (growth media; n = 3) stands for myoblasts in the proliferative phase. DM (differentiation media; n = 3), DM1-DM6 means differentiation from day 1 to day 6.

    Techniques Used: Methylation, Modification, Expressing, Transfection, Luciferase, Reporter Assay, Amplification, Over Expression, Binding Assay, Mass Spectrometry, Western Blot

    Related Articles

    Over Expression:

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    Plasmid Preparation:

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    Article Title: LncRNA TUG1 promotes hypertrophic scar formation via the miR-627/IGF1R axis.
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    Article Title: BVDV hijacks the IDO1-dependent tryptophan-kynurenine axis to promote viral replication by limiting interferon-STAT1 antiviral signaling.
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    Article Title: RPSA-OLFM4 axis governs neutrophil migration against bacterial infection and sepsis.
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    Small Interfering RNA:

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    Control:

    Article Title: HIRA promotes osteogenic differentiation of BMSCs and ameliorates osteoporosis by mediating M2 polarization of macrophages through the YAP1/β-catenin pathway.
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    Article Title: 5-Methoxyflavone suppresses nasopharyngeal carcinoma proliferation through regulating p53/SREBP/SCD signaling-mediated lipid synthesis and ferroptosis.
    Article Snippet: p53 siRNA and SREBP1siRNA, along with a negative control, were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). .. SCD overexpression plasmid and the empty vector control were also purchased from Sangon Biotech Co., Ltd.. NPC cells were cultured in 6-well plates and transfected with siRNA or plasmid using the jetPRIME Transfection Reagent when they reached 60%-70% confluence. ..

    Article Title: LncRNA TUG1 promotes hypertrophic scar formation via the miR-627/IGF1R axis.
    Article Snippet: .. The TUG1 overexpression plasmid (pcDNA3.1-TUG1) and corresponding empty vector control (pcDNA3.1) were obtained from Sangon Biotech (Shanghai, China). .. Transfections were performed using LipofectamineTM 2000 Transfection Reagent (1.5 mL; Cat. No. 11668019; Thermo Fisher Scientific, USA) according to the manufacturer’s instructions.

    Construct:

    Article Title: HIRA promotes osteogenic differentiation of BMSCs and ameliorates osteoporosis by mediating M2 polarization of macrophages through the YAP1/β-catenin pathway.
    Article Snippet: Extended author information available on the last page of the article Abstract Impaired macrophage polarization is a key factor exacerbating osteoporosis (OP), influencing bone metabolism via modulating the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).. Histone cell cycle regulator (HIRA), an epigenetic regulator, is downregulated in OP.. However, its role in modulating macrophage polarization and BMSCs osteogenic differentiation remains unclear.

    Article Title: RPSA-OLFM4 axis governs neutrophil migration against bacterial infection and sepsis.
    Article Snippet: Plasmids and transfection Overexpression plasmid OLFM4-3×FLAG-CMV-10 was synthesized by Sangon Biotech Co., Ltd (Shanghai, China). .. OLFM4 shRNA plasmid (CMV.G&PR.U6.shRNA) and RPSA shRNA plasmid (pLKO.1-TRC) were AR TI CL E IN P RE SS constructed by Sangon Biotech Co., Ltd. Plasmids were transfected into cells using lipofectamine 2000 (Invitrogen, Grand Island, NY, USA), according to the manufacturer’s instructions. ..

    shRNA:

    Article Title: RPSA-OLFM4 axis governs neutrophil migration against bacterial infection and sepsis.
    Article Snippet: Plasmids and transfection Overexpression plasmid OLFM4-3×FLAG-CMV-10 was synthesized by Sangon Biotech Co., Ltd (Shanghai, China). .. OLFM4 shRNA plasmid (CMV.G&PR.U6.shRNA) and RPSA shRNA plasmid (pLKO.1-TRC) were AR TI CL E IN P RE SS constructed by Sangon Biotech Co., Ltd. Plasmids were transfected into cells using lipofectamine 2000 (Invitrogen, Grand Island, NY, USA), according to the manufacturer’s instructions. ..

    Transfection:

    Article Title: RPSA-OLFM4 axis governs neutrophil migration against bacterial infection and sepsis.
    Article Snippet: Plasmids and transfection Overexpression plasmid OLFM4-3×FLAG-CMV-10 was synthesized by Sangon Biotech Co., Ltd (Shanghai, China). .. OLFM4 shRNA plasmid (CMV.G&PR.U6.shRNA) and RPSA shRNA plasmid (pLKO.1-TRC) were AR TI CL E IN P RE SS constructed by Sangon Biotech Co., Ltd. Plasmids were transfected into cells using lipofectamine 2000 (Invitrogen, Grand Island, NY, USA), according to the manufacturer’s instructions. ..

    Article Title: 5-Methoxyflavone suppresses nasopharyngeal carcinoma proliferation through regulating p53/SREBP/SCD signaling-mediated lipid synthesis and ferroptosis.
    Article Snippet: p53 siRNA and SREBP1siRNA, along with a negative control, were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). .. SCD overexpression plasmid and the empty vector control were also purchased from Sangon Biotech Co., Ltd.. NPC cells were cultured in 6-well plates and transfected with siRNA or plasmid using the jetPRIME Transfection Reagent when they reached 60%-70% confluence. ..

    Article Title: RPSA-OLFM4 axis governs neutrophil migration against bacterial infection and sepsis.
    Article Snippet: .. Plasmids and transfection Overexpression plasmid OLFM4-3×FLAG-CMV-10 was synthesized by Sangon Biotech Co., Ltd (Shanghai, China). .. OLFM4 shRNA plasmid (CMV.G&PR.U6.shRNA) and RPSA shRNA plasmid (pLKO.1-TRC) were AR TI CL E IN P RE SS constructed by Sangon Biotech Co., Ltd. Plasmids were transfected into cells using lipofectamine 2000 (Invitrogen, Grand Island, NY, USA), according to the manufacturer’s instructions.

    Cell Culture:

    Article Title: 5-Methoxyflavone suppresses nasopharyngeal carcinoma proliferation through regulating p53/SREBP/SCD signaling-mediated lipid synthesis and ferroptosis.
    Article Snippet: p53 siRNA and SREBP1siRNA, along with a negative control, were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). .. SCD overexpression plasmid and the empty vector control were also purchased from Sangon Biotech Co., Ltd.. NPC cells were cultured in 6-well plates and transfected with siRNA or plasmid using the jetPRIME Transfection Reagent when they reached 60%-70% confluence. ..

    Sequencing:

    Article Title: The miR-214-3p/ CTSD Axis Regulates Lysosomal Homeostasis in Porcine Intestinal Epithelial Cells: A Preliminary Study.
    Article Snippet: .. The cDNA sequence of porcine CTSD was cloned into the pcDNA3.1(+) vector (Sangon Biotech) to generate the CTSD overexpression plasmid (pcDNA-CTSD), with the empty pcDNA3.1(+) vector served as a negative control. ..

    Clone Assay:

    Article Title: The miR-214-3p/ CTSD Axis Regulates Lysosomal Homeostasis in Porcine Intestinal Epithelial Cells: A Preliminary Study.
    Article Snippet: .. The cDNA sequence of porcine CTSD was cloned into the pcDNA3.1(+) vector (Sangon Biotech) to generate the CTSD overexpression plasmid (pcDNA-CTSD), with the empty pcDNA3.1(+) vector served as a negative control. ..

    Negative Control:

    Article Title: The miR-214-3p/ CTSD Axis Regulates Lysosomal Homeostasis in Porcine Intestinal Epithelial Cells: A Preliminary Study.
    Article Snippet: .. The cDNA sequence of porcine CTSD was cloned into the pcDNA3.1(+) vector (Sangon Biotech) to generate the CTSD overexpression plasmid (pcDNA-CTSD), with the empty pcDNA3.1(+) vector served as a negative control. ..

    Synthesized:

    Article Title: RPSA-OLFM4 axis governs neutrophil migration against bacterial infection and sepsis.
    Article Snippet: .. Plasmids and transfection Overexpression plasmid OLFM4-3×FLAG-CMV-10 was synthesized by Sangon Biotech Co., Ltd (Shanghai, China). .. OLFM4 shRNA plasmid (CMV.G&PR.U6.shRNA) and RPSA shRNA plasmid (pLKO.1-TRC) were AR TI CL E IN P RE SS constructed by Sangon Biotech Co., Ltd. Plasmids were transfected into cells using lipofectamine 2000 (Invitrogen, Grand Island, NY, USA), according to the manufacturer’s instructions.



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