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assay  (Dojindo Labs)


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    Structured Review

    Dojindo Labs assay
    Assay, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/viability/Microbial+Viability+Assay+Kit-WST/pmc12349227-98-19-24
    Average 95 stars, based on 121 article reviews
    assay - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Viability Assay:

    Article Title: Enhanced Delivery of Antimicrobial Peptide via Dual-Functionalized Silica Nanoparticles Achieves Efficient Staphylococcus aureus Biofilm Eradication.
    Article Snippet: Tryptic Soy Broth (Merck), Tryptic Soy Agar (Merck), glucose (pharma grade, Conda), and phosphate- buffered saline (PBS, Sigma- Aldrich) were used in biofilm penetration and eradication studies. .. WST- 8 Microbial Viability Assay Kit was purchased from Dojindo Molecular Technologies. .. FilmTracer FM 1- 43 green biofilm cell stain was purchased from Invitrogen for biomass quantification.

    Article Title: Xylitol Modification of Electrospun Polymer Scaffolds: Impact on Physicochemical and Antibacterial Properties
    Article Snippet: .. In this experiment, the WST-8 kit (Microbial Viability Assay Kit-WST, Dojindo, Kumamoto, Japan) was used as a calorimetric indicator which releases a water-soluble formazan dye upon reduction in the presence of an electron mediator. ..

    Article Title: Natural Antimicrobial Activity of Nettle ( Urtica dioica L.) Leaf Extract for Shelf‐Life Extension of Mashed Potatoes
    Article Snippet: .. The minimum inhibitory concentration (MIC) was determined using the Microbial Viability Assay Kit (Dojindo Molecular Technologies, Kumamoto, Japan), according to manufacturer instructions and as slightly modified by Kramer et al. ( ). ..

    Article Title: Sunlight-activated semiconducting iron minerals in anammox granules: Stimulating metabolic cooperation, suppressing nitrate accumulation, and mitigating oxidative stress.
    Article Snippet: • Sunlight-activated iron minerals suppress nitrate formation by up to 43 %.. • Sunlight-activated iron minerals stimulates Ca.. Brocadia activity.

    Article Title: Anti-Biofilm Activity of Combinations of Cinnamic Acid and Its Derivatives with Cloxacillin Against Methicillin-Resistant Staphylococcus epidermidis
    Article Snippet: .. After incubation, the mass of the formed biofilm was evaluated using a modified Christensen method [ ], and cell viability was assessed using the Microbial Viability Assay Kit-WST according to the manufacturer’s instructions (Dojindo Laboratories, Kumamoto, Japan). ..

    Article Title: Culture-Free Microfluidics for Ultra-Rapid Antimicrobial Susceptibility Testing with AI in Resource-Limited Settings.
    Article Snippet: The misuse of antibiotics has accelerated the rise of antimicrobial resistance (AMR), particularly in resource-limited regions, posing a critical threat to global public health.. Current antibiotic susceptibility test (AST) technologies are trapped in a paradox: regions most in need of rapid diagnostics face the greatest implementation barriers due to culture-based delays and infrastructure dependence.. No existing platform simultaneously overcomes these spatiotemporal constraints while maintaining phenotypic reliability.

    Article Title: Anti-Biofilm Activity of Combinations of Cinnamic Acid and Its Derivatives with Cloxacillin Against Methicillin-Resistant Staphylococcus epidermidis
    Article Snippet: .. After incubation at a temperature of 37 ± 1 °C for a period of 16 ± 2 h under aerobic conditions, the culture medium was removed, and viability was determined using the Microbial Viability Assay Kit-WST according to the manufacturer’s instructions (Dojindo Laboratories, Kumamoto, Japan) [ ]. .. The analysis was performed using a Multiskan EX Microplate Reader (Thermo Electron Corp., Vantaa, Finland).

    Article Title: Development of an Alternative to Preservation Efficacy Tests Using the Metabolic Activity of Microorganisms.
    Article Snippet: .. Then, in a new 96-well flat plate, 170 μl of Soybean-Casein Digest Broth (SCDB) from Nihon Pharmaceutical Co., Ltd., 20 μl of the sample di luted with LP, and 10 μl of color reagent (DOJINDO LABORATORIES: Microbial Viability Assay Kit-WST) were sequentially mixed. .. The plate was sealed with an Adhesive PCR Plate Seal (Thermo Fisher Scientific: AB0558) to prevent evaporation.

    Concentration Assay:

    Article Title: Natural Antimicrobial Activity of Nettle ( Urtica dioica L.) Leaf Extract for Shelf‐Life Extension of Mashed Potatoes
    Article Snippet: .. The minimum inhibitory concentration (MIC) was determined using the Microbial Viability Assay Kit (Dojindo Molecular Technologies, Kumamoto, Japan), according to manufacturer instructions and as slightly modified by Kramer et al. ( ). ..

    Modification:

    Article Title: Natural Antimicrobial Activity of Nettle ( Urtica dioica L.) Leaf Extract for Shelf‐Life Extension of Mashed Potatoes
    Article Snippet: .. The minimum inhibitory concentration (MIC) was determined using the Microbial Viability Assay Kit (Dojindo Molecular Technologies, Kumamoto, Japan), according to manufacturer instructions and as slightly modified by Kramer et al. ( ). ..

    Article Title: Anti-Biofilm Activity of Combinations of Cinnamic Acid and Its Derivatives with Cloxacillin Against Methicillin-Resistant Staphylococcus epidermidis
    Article Snippet: .. After incubation, the mass of the formed biofilm was evaluated using a modified Christensen method [ ], and cell viability was assessed using the Microbial Viability Assay Kit-WST according to the manufacturer’s instructions (Dojindo Laboratories, Kumamoto, Japan). ..

    Incubation:

    Article Title: Anti-Biofilm Activity of Combinations of Cinnamic Acid and Its Derivatives with Cloxacillin Against Methicillin-Resistant Staphylococcus epidermidis
    Article Snippet: .. After incubation, the mass of the formed biofilm was evaluated using a modified Christensen method [ ], and cell viability was assessed using the Microbial Viability Assay Kit-WST according to the manufacturer’s instructions (Dojindo Laboratories, Kumamoto, Japan). ..

    Article Title: Anti-Biofilm Activity of Combinations of Cinnamic Acid and Its Derivatives with Cloxacillin Against Methicillin-Resistant Staphylococcus epidermidis
    Article Snippet: .. After incubation at a temperature of 37 ± 1 °C for a period of 16 ± 2 h under aerobic conditions, the culture medium was removed, and viability was determined using the Microbial Viability Assay Kit-WST according to the manufacturer’s instructions (Dojindo Laboratories, Kumamoto, Japan) [ ]. .. The analysis was performed using a Multiskan EX Microplate Reader (Thermo Electron Corp., Vantaa, Finland).

    Activity Assay:

    Article Title: Culture-Free Microfluidics for Ultra-Rapid Antimicrobial Susceptibility Testing with AI in Resource-Limited Settings.
    Article Snippet: The misuse of antibiotics has accelerated the rise of antimicrobial resistance (AMR), particularly in resource-limited regions, posing a critical threat to global public health.. Current antibiotic susceptibility test (AST) technologies are trapped in a paradox: regions most in need of rapid diagnostics face the greatest implementation barriers due to culture-based delays and infrastructure dependence.. No existing platform simultaneously overcomes these spatiotemporal constraints while maintaining phenotypic reliability.



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    Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. <t>A)</t> <t>Calcein/PI</t> staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.
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    Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. <t>A)</t> <t>Calcein/PI</t> staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.
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    Image Search Results


    Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

    Journal: Bioactive Materials

    Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

    doi: 10.1016/j.bioactmat.2026.02.048

    Figure Lengend Snippet: Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

    Article Snippet: A Calcein/PI Cell Viability/Cytotoxicity Assay Kit (Beyotime, China) was used to recognize the living and dead cells.

    Techniques: In Vitro, Staining, Migration, Negative Control, Cell Culture, Software, Control