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Narishige inc micropipette puller
Micropipette Puller, supplied by Narishige inc, used in various techniques. Bioz Stars score: 96/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vertical+puller/Narishige+PC-100+Vertical+puller/pm41986345-461-14-17
Average 96 stars, based on 158 article reviews
micropipette puller - by Bioz Stars, 2026-10
96/100 stars

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Article Title: A pharynx-to-brain sensory pathway mediates swallowing-like behavior evoked by mechanical stimulation
Article Snippet: Sectioning was performed on a vibratome (VT1200S, Leica Microsystems, Germany), and the resultant slices were allowed to recover for 30min at 28 °C in oxygenated artificial cerebrospinal fluid (ACSF) containing (inmM) 119 NaCl, 2.5 KCl, 1 NaH2PO4, 1.3 MgSO4, 26 NaHCO3, 10 glucose, and 2.5 CaCl2. .. Patch electrodes were fabricated fromborosilicate glass capillaries (Warner Instruments) using a PC-100 puller (Narishige, Japan). ..

Article Title: Molecular mechanisms of lipid sensing in human cholecystokinin-secreting enteroendocrine cells.
Article Snippet: .. Patch pipettes were pulled from borosilicate glass (1.5 mm OD, 1.17 mm ID, Harvard Apparatus) using a PC-100 puller (Narishige) and had resistances of 2.5-3.5 MΩ when filled with pipette solution. ..

Article Title: Reduction of the Fast Sodium Current in the Myocardium of Fish (Nothobranchius furzeri) during Aging.
Article Snippet: Aging is associated with physiological remodeling of the organism, including cardiac tissue, and an increased risk of arrhythmias.. We analyzed age-related changes in cardiomyocyte morphology and the fast sodium current (INa), which determines cardiac excitability and conduction velocity, in the ventricular myocardium of the short-lived fish Nothobranchius furzeri.. Isolated cardiomyocytes from young (2-month-old) and aged (4-month-old) fish were examined using light and fluorescence microscopy and the whole-cell patch-clamp technique.

Article Title: Unraveling the interfacial interaction mechanisms of CO2 bubble-assisted oil liberation from quartz surfaces
Article Snippet: Understanding the mechanisms governing bubble-oil-mineral interfacial interactions is crucial for optimizing engineering processes such as environmental remediation, oil recovery, and oil sand extraction.. However, how CO2 bubbles regulate interfacial forces during oil removal from mineral surfaces remains poorly understood.. In this work, we applied atomic force microscopy bubble/drop probe technique to investigate the interfacial interactions of CO2 bubbles, dodecane drops, and quartz surfaces in aqueous solution.

Article Title: Study on nonlinear spatial stitching method of SICM images based on adaptive region candidates.
Article Snippet: Scanning Ion Conductance Microscopy (SICM) can realize three-dimensional, non-invasive imaging of living biological cells, and has made significant progress in fields such as cell biology and medicine.. However, constrained by the maximum motion range of the piezoelectric actuator (e.g., 100 μm × 100 μm), it always makes high-precision large-scale scanning technically challenging.. In order to address this problem, this study proposes a non-linear spatial stitching method for SICM images based on adaptive region candidates.

Article Title: Nectin3 on prefrontal excitatory and inhibitory neurons distinctly mediates adolescent stress-induced cognitive and social deficits
Article Snippet: .. Borosilicate glass capillaries were pulled into patch pipettes with a resistance of 3–5 MΩ using a two-stage PC-100 puller (Narishige). ..

Article Title: Long-lasting survival, migration and functional maturation of transplanted human reprogrammed neurons in vivo.
Article Snippet: .. Patch electrodes (5–7 MΩ) were pulled from borosilicate glass using a Narishige PC-100 vertical pipette puller and filled with an intracellular solution containing (in mM): 130 Kgluconate, 10 KCl, 0.2 EGTA, 10 HEPES, 4 Mg-ATP, 0.5 Na-GTP, and 10 Na-phosphocreatine (pH 7.25, 296 mOsm/kg). .. Recordings were obtained using a Multiclamp 700B amplifier and pClamp 11 software (Molecular Devices, USA).

Transferring:

Article Title: Molecular mechanisms of lipid sensing in human cholecystokinin-secreting enteroendocrine cells.
Article Snippet: .. Patch pipettes were pulled from borosilicate glass (1.5 mm OD, 1.17 mm ID, Harvard Apparatus) using a PC-100 puller (Narishige) and had resistances of 2.5-3.5 MΩ when filled with pipette solution. ..

Article Title: Unraveling the interfacial interaction mechanisms of CO2 bubble-assisted oil liberation from quartz surfaces
Article Snippet: Understanding the mechanisms governing bubble-oil-mineral interfacial interactions is crucial for optimizing engineering processes such as environmental remediation, oil recovery, and oil sand extraction.. However, how CO2 bubbles regulate interfacial forces during oil removal from mineral surfaces remains poorly understood.. In this work, we applied atomic force microscopy bubble/drop probe technique to investigate the interfacial interactions of CO2 bubbles, dodecane drops, and quartz surfaces in aqueous solution.

Article Title: Study on nonlinear spatial stitching method of SICM images based on adaptive region candidates.
Article Snippet: Scanning Ion Conductance Microscopy (SICM) can realize three-dimensional, non-invasive imaging of living biological cells, and has made significant progress in fields such as cell biology and medicine.. However, constrained by the maximum motion range of the piezoelectric actuator (e.g., 100 μm × 100 μm), it always makes high-precision large-scale scanning technically challenging.. In order to address this problem, this study proposes a non-linear spatial stitching method for SICM images based on adaptive region candidates.

Article Title: Long-lasting survival, migration and functional maturation of transplanted human reprogrammed neurons in vivo.
Article Snippet: .. Patch electrodes (5–7 MΩ) were pulled from borosilicate glass using a Narishige PC-100 vertical pipette puller and filled with an intracellular solution containing (in mM): 130 Kgluconate, 10 KCl, 0.2 EGTA, 10 HEPES, 4 Mg-ATP, 0.5 Na-GTP, and 10 Na-phosphocreatine (pH 7.25, 296 mOsm/kg). .. Recordings were obtained using a Multiclamp 700B amplifier and pClamp 11 software (Molecular Devices, USA).

Generated:

Article Title: Unraveling the interfacial interaction mechanisms of CO2 bubble-assisted oil liberation from quartz surfaces
Article Snippet: Understanding the mechanisms governing bubble-oil-mineral interfacial interactions is crucial for optimizing engineering processes such as environmental remediation, oil recovery, and oil sand extraction.. However, how CO2 bubbles regulate interfacial forces during oil removal from mineral surfaces remains poorly understood.. In this work, we applied atomic force microscopy bubble/drop probe technique to investigate the interfacial interactions of CO2 bubbles, dodecane drops, and quartz surfaces in aqueous solution.



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