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Amersham Life Sciences Inc hybridization buffer version 2
Hybridization Buffer Version 2, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+2+hybridization+buffer/hybridization+buffer/pm12790802-53-7-11
Average 90 stars, based on 1 article reviews
hybridization buffer version 2 - by Bioz Stars, 2026-09
90/100 stars

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Article Title: In Vivo Occupancy of Mitochondrial Single-Stranded DNA Binding Protein Supports the Strand Displacement Mode of DNA Replication
Article Snippet: Southern blot analysis of 2D gels were hybridized to a specific region of human mtDNA (mtDNA 13636-1006) by 2 hrs incubation at 65°C in hybridizing buffer (Amersham rapid-hyb buffer) under conditions recommended by the manufacturer (GE Healthcare).

Incubation:

Article Title: Prolonged Cyclic Strain Inhibits Human Endothelial Cell Growth
Article Snippet: .. RNA was blot transferred to Gene Screen Plus membranes and then incubated overnight at 68°C in hybridization buffer (Amersham, Arlington Heights, IL) containing [ 32 P]DNA probes (1×10 8 cpm) for p21 or GAPDH. ..

Hybridization:

Article Title: Prolonged Cyclic Strain Inhibits Human Endothelial Cell Growth
Article Snippet: .. RNA was blot transferred to Gene Screen Plus membranes and then incubated overnight at 68°C in hybridization buffer (Amersham, Arlington Heights, IL) containing [ 32 P]DNA probes (1×10 8 cpm) for p21 or GAPDH. ..

Article Title: In vivo functional analysis of a nuclear restorer PPR protein
Article Snippet: 15ug samples from individual plants were digested with EcoR1 and eletrophoresed on a 1% agarose gel at 20 V for 20 hr, transferred to a charged nylon membrane (Amersham/GE Healthcare, Baie d’Urfe, QC). .. After 3 hr of blocking at 55°C with hybridization buffer plus blocking reagent (Amersham) and NaCl 0.5 M, a probe encompassing the wtRfo promoter and coding sequences was labeled with α- 32 PdCTP using the Prime-a-Gene Labeling system (Promega, Madison WI, USA). ..

Article Title: Spatial behavior and seizure-induced changes in c-fos mRNA expression in young and old rats
Article Snippet: Sections were incubated with 100 μL 1X prehybridization buffer (Sigma, St. Louis, MO, USA) for 30 minutes at room temperature. .. Approximately 100 ng of riboprobe was diluted in 1X enhanced hybridization buffer (Amersham, Piscataway, NJ, USA), heat denatured at 90 °C, chilled on ice, and applied to each section. ..

Article Title: Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol
Article Snippet: The mtlD probe (285-bp), labelled with thermostable alkaline phosphatase, using alkaphos direct labelling kit (Amersham, GE Healthcare, UK), was used for hybridization. .. Hybridization was carried out overnight at 55°C in hybridization buffer (Amersham, GE Healthcare, UK) and membrane was washed (at 55°C for 15 min) first in primary wash buffer (25 mL) and then in secondary wash buffer at room temperature. .. Hybridized membrane was detected by using CDP-star chemiluminescent as substrate and signals were visualised on Amersham Hyperfilm ECL after 1 h.

Article Title: Behavior-driven Arc expression is reduced in all ventral hippocampal subfields compared to CA1, CA3 and dentate gyrus in rat dorsal hippocampus
Article Snippet: Sections were incubated with 100 μl 1× prehybridization buffer (Sigma, St. Louis, MS) for 30 min at room temperature. .. Approximately 100 ng of riboprobe was diluted in 1× hybridization buffer (Amersham, Piscataway, NJ), heat denatured at 90° C, chilled on ice and applied to each section. ..

Article Title: Characterization and RNA-seq analysis of underperformer, an activation-tagged potato mutant.
Article Snippet: Advances in genomic technology have allowed the sequencing and annotation of numerous plant genomes.. Potato (Solanum tuberosum) is the 30th plant species and first asterid for which a genome sequence has been produced, providing significant new information on potato gene models.. The genome size of the doubled monoploid potato (S. tuberosum Group Phureja DM 1-3 566 R44) selected for sequencing is 844 megabases (Mb), and the assembly and annotation of 86 % of this 844 Mb have predicted 39,031 protein coding genes and a 62.2 % repetitive DNA content (The Potato Genome Sequencing Consortium 2011).

Article Title: Hippocampal Synaptic Expansion Induced by Spatial Experience in Rats Correlates with Improved Information Processing in the Hippocampus
Article Snippet: .. The Arc riboprobe was diluted into 100 μL of hybridization buffer (Amersham), denatured by heating at 95°C for 5 min, and then chilled on ice until it was added to each slide (100 ng riboprobe per slide section). ..

Blocking Assay:

Article Title: In vivo functional analysis of a nuclear restorer PPR protein
Article Snippet: 15ug samples from individual plants were digested with EcoR1 and eletrophoresed on a 1% agarose gel at 20 V for 20 hr, transferred to a charged nylon membrane (Amersham/GE Healthcare, Baie d’Urfe, QC). .. After 3 hr of blocking at 55°C with hybridization buffer plus blocking reagent (Amersham) and NaCl 0.5 M, a probe encompassing the wtRfo promoter and coding sequences was labeled with α- 32 PdCTP using the Prime-a-Gene Labeling system (Promega, Madison WI, USA). ..

Labeling:

Article Title: In vivo functional analysis of a nuclear restorer PPR protein
Article Snippet: 15ug samples from individual plants were digested with EcoR1 and eletrophoresed on a 1% agarose gel at 20 V for 20 hr, transferred to a charged nylon membrane (Amersham/GE Healthcare, Baie d’Urfe, QC). .. After 3 hr of blocking at 55°C with hybridization buffer plus blocking reagent (Amersham) and NaCl 0.5 M, a probe encompassing the wtRfo promoter and coding sequences was labeled with α- 32 PdCTP using the Prime-a-Gene Labeling system (Promega, Madison WI, USA). ..

Membrane:

Article Title: Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol
Article Snippet: The mtlD probe (285-bp), labelled with thermostable alkaline phosphatase, using alkaphos direct labelling kit (Amersham, GE Healthcare, UK), was used for hybridization. .. Hybridization was carried out overnight at 55°C in hybridization buffer (Amersham, GE Healthcare, UK) and membrane was washed (at 55°C for 15 min) first in primary wash buffer (25 mL) and then in secondary wash buffer at room temperature. .. Hybridized membrane was detected by using CDP-star chemiluminescent as substrate and signals were visualised on Amersham Hyperfilm ECL after 1 h.



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