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dapi  (Vector Laboratories)


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  • 99

    Structured Review

    Vector Laboratories dapi
    Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 9998 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+shield+with+dapi/Streptavidin/pmc05890188-67-15-23
    Average 99 stars, based on 9998 article reviews
    dapi - by Bioz Stars, 2026-10
    99/100 stars

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    Incubation:

    Article Title: Hippo signaling pathway in human testis and seminoma: anticancer effect of verteporfin on human seminoma TCam-2 cells.
    Article Snippet: 1 Department of Histology and Embryology, Faculty of Medicine, Demiroğlu Bilim University, 34394 Istanbul, Turkey 2 Department of Immunology, Yeditepe University Faculty of Medicine, 34755 Istanbul, Turkey 3 Department of Histology and Embryology, Faculty of Medicine, University of Health Sciences, 34668 Istanbul, Turkey 4 Department of Pathology, Samatya Education and Research Hospital, Istanbul, Turkey 5 Department of Histology and Embryology, Yeditepe University Faculty of Medicine, 34755 Istanbul, Turkey Abstract Aim Seminoma and embryonal carcinoma are among the most common germ cell tumors.. Verteporfin, a YAP-TEAD inhibitor, has emerged as a potential anticancer agent.. This study investigated the localization of Hippo signaling proteins in human testis, seminoma, and non-seminoma tissues, and examined verteporfin’s effects on TCam-2 seminoma cells.

    Article Title: Mechanotransducer Piezo1 drives ventilator‑induced lung injury in lung epithelial cells via the calcineurin/NFATc3 pathway.
    Article Snippet: After deparaffinization and rehydration, antigen retrieval was performed by heating the sections in citrate buffer (pH 6.0) at 95 ̊C for 20 min. Endogenous peroxidase activity was blocked by incubating the sections with 3% hydrogen peroxide in methanol for 10 min at room temperature, and the sections were then incubated with 5% normal goat serum (cat. no. S‐1000; Vector laboratories; Maravai lifeSciences) in PBS for 1 h at room temperature to block non‐specific binding sites. .. The sections were then incubated overnight at 4 ̊C with primary antibody against Piezo1 (1:200). after washing with PBS, the sections were incubated with biotin‐conjugated goat anti‐rabbit igG secondary antibody (1:200; cat. no. Ba‐1000; Vector laboratories; Maravai lifeSciences) for 30 min at room temperature, followed by incubation with streptavidin‐horse‐ radish peroxidase conjugate (1:500; cat. no. Sa‐5004; Vector laboratories; Maravai lifeSciences) for 30 min at room temperature. immunoreactivity was visualized using daB substrate kit (cat. no. SK‐4100; Vector laboratories; Maravai lifeSciences) according to the manufacturer's instructions. ..

    Article Title: Interstitial palladin is associated with tubulointerstitial chronicity and adverse outcomes in patients with lupus nephritis.
    Article Snippet: .. For colocalization of palladin and α-smooth muscle actin (αSMA), sections were incubated with anti-palladin antibody, biotinylated anti-rabbit IgG, and streptavidin DyLight 594 (Vector Laboratories, Burlingame, CA). .. After avidin/biotin blocking, sections were incubated with mouse anti-αSMA antibody (Santa Cruz Biotechnology, Santa Cruz, CA), biotinylated anti-mouse IgG, and streptavidin DyLight 488.

    Article Title: Therapeutic potential of polygonatum polysaccharides in modulating gut microbiota and alleviating psoriasis-like inflammation in a mouse model.
    Article Snippet: 1 Department of Pharmacy, The Second Affiliated Hospital, The Domestic First-class Discipline Construction Project of Hunan University of Chinese Medicine, Changsha 410005, Hunan, China 2 Department of Dermatology, The Second Affiliated Hospital, The Domestic First-class Discipline Construction Project of Hunan University of Chinese Medicine, Changsha 410005, Hunan, China 3 Hunan Engineering Technology Research Center for Medicinal and Functional Food, Hunan University of Chinese Medicine, Changsha 410208, China 4 Department of Scientific Research, The Second Affiliated Hospital, The Domestic First-class Discipline Construction Project of Hunan University of Chinese Medicine, Changsha 410005, Hunan, China 5 Hunan Aerospace Hospital Health Management Center, Changsha 410205, China Abstract Psoriasis is a chronic inflammatory skin disorder characterized by keratinocyte hyperproliferation and immune dysregulation.. The gut microbiota has emerged as a significant factor influencing the pathogenesis of psoriasis, particularly through the gut-skin axis.. This study investigates the therapeutic effects of Polygonatum polysaccharides (PSP) on imiquimod (IMQ)-induced psoriasis-like dermatitis in mice, focusing on gut microbiota modulation and systemic immune responses.

    Article Title: Effects of Acute Inflammation Induced by Lipopolysaccharide (LPS) and Pam3csk4 (TLR2/1 Agonist) on Anti-Müllerian Hormone (AMH) Expression and Serum Levels in the Mouse Ovary
    Article Snippet: After primary antibody incubation, the sections were washed with PBS (pH 7.2-7.4) and incubated with goat antimouse IgG secondary antibody (BA-9200; Vector Laboratories Inc., Burlingame, CA, USA) at a dilution of 1:100. .. This was followed by incubation with the streptavidin-peroxidasecontaining Vectastain ABC system (Vector Laboratories) for 30 minutes. .. Visualization of antigen-antibody complexes was achieved using Liquid DAB+ substrate kit (Agilent Dako, Santa Clara, CA, USA).

    Article Title: Enzyme‐Linked Cycloaddition Assay (ELCA) for Rapid, Ultra‐Sensitive Monitoring of Secreted Sialoglycoproteins
    Article Snippet: .. Detached cells were washed once in PBS and then incubated with 2 μg/mL streptavidin‐fluorescein (SA‐5001‐1, Vector Laboratories) for 30 min at 4°C. .. Surface staining was done together with the LIVE/DEAD Fixable Far Red Dead Cell Stain Kit ( L10120 , Thermo Fisher, 1000× dilution).

    other:

    Article Title: Chemotherapy triggers immune evasion by fostering LEPR + Kupffer cell differentiation in liver metastases.
    Article Snippet: Article Chemotherapy triggers immune evasion by fostering LEPR + Kupffer cell differentiation in liver metastases

    Blocking Assay:

    Article Title: Therapeutic potential of polygonatum polysaccharides in modulating gut microbiota and alleviating psoriasis-like inflammation in a mouse model.
    Article Snippet: 1 Department of Pharmacy, The Second Affiliated Hospital, The Domestic First-class Discipline Construction Project of Hunan University of Chinese Medicine, Changsha 410005, Hunan, China 2 Department of Dermatology, The Second Affiliated Hospital, The Domestic First-class Discipline Construction Project of Hunan University of Chinese Medicine, Changsha 410005, Hunan, China 3 Hunan Engineering Technology Research Center for Medicinal and Functional Food, Hunan University of Chinese Medicine, Changsha 410208, China 4 Department of Scientific Research, The Second Affiliated Hospital, The Domestic First-class Discipline Construction Project of Hunan University of Chinese Medicine, Changsha 410005, Hunan, China 5 Hunan Aerospace Hospital Health Management Center, Changsha 410205, China Abstract Psoriasis is a chronic inflammatory skin disorder characterized by keratinocyte hyperproliferation and immune dysregulation.. The gut microbiota has emerged as a significant factor influencing the pathogenesis of psoriasis, particularly through the gut-skin axis.. This study investigates the therapeutic effects of Polygonatum polysaccharides (PSP) on imiquimod (IMQ)-induced psoriasis-like dermatitis in mice, focusing on gut microbiota modulation and systemic immune responses.



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