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vectashield mounting medium  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vectashield mounting medium
    Vectashield Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 25513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+shield+with+dapi/VECTASHIELD+Antifade+Mounting+Medium/pm23869968-222-5-12
    Average 99 stars, based on 25513 article reviews
    vectashield mounting medium - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: A novel homozygous missense ODF2 variant in infertile siblings with multiple morphological abnormalities of the sperm flagella.
    Article Snippet: .. Fixed cells were mounted using Vectashield mounting medium (H-1000; Vector Laboratories, Burlingame, CA, USA), and images were acquired using an Olympus BX53 microscope (Olympus) equipped with an SC-180 camera and a phase contrast channel. ..

    Article Title: Early post-sepsis innate behavioral changes in aged mice occur independently of detectable neuroinflammation.
    Article Snippet: NucBlue DAPI stain (Invitrogen, R37606) was added and incubated for 20 additional minutes. .. Finally, sections were rinsed in PBS twice, 5 min each, and mounted on microscope slides using Vectashield Antifade Mounting Medium (Vector Laboratories, H1000-10). .. Zeiss LSM710 confocal microscope was used to capture images of the prefrontal cortex and hippocampus at 20X magnification using Zeiss ZEN 3.4.

    Phase Contrast:

    Article Title: A novel homozygous missense ODF2 variant in infertile siblings with multiple morphological abnormalities of the sperm flagella.
    Article Snippet: .. Fixed cells were mounted using Vectashield mounting medium (H-1000; Vector Laboratories, Burlingame, CA, USA), and images were acquired using an Olympus BX53 microscope (Olympus) equipped with an SC-180 camera and a phase contrast channel. ..

    Staining:

    Article Title: Modulation of β‐Catenin/ EMT Axis by Croton caudatus and Its Phytoflavonoid Kaempferol Ameliorates Cervical Cancer Migration and Stemness
    Article Snippet: Briefly, HeLa and Siha cells were fixed with 4% (w/v) paraformaldehyde, washed, permeabilised with 0.1% (v/v) Triton X- 100 and blocked with 3% (w/v) BSA, followed by incubation with β- catenin polyclonal primary antibody (Cell signaling technology, USA) overnight at 4°C and next with Alexa Fluor 488 fluorophore- conjugated Goat Anti- Mouse IgG secondary antibody (BioLegend, USA) and Alexa Fluor 546 fluorophore- conjugated Goat Anti- Rabbit IgG secondary antibody (BioLegend, USA). .. Nucleus were stained with DAPI (Sigma Aldrich, USA) and cells were mounted with Vectashield (Vector Laboratories, USA, CA) mounting reagent. .. Images were captured using Leica TCA SP8 confocal microscope (Leica S5 Microsystems, Wetzlar, Germany, RRID:SCR_027226).

    Article Title: Biomanufacturing Vascular Matrix-Mimetic Hydrogels from Quantified Arterial Micromechanics and Microstructure.
    Article Snippet: The microstructural and micromechanical properties of the arterial extracellular matrix (ECM) are central to vascular cell behavior but are disrupted by disease and aging.. Elastin and fibrillar collagens provide essential cues, yet the effects of disease-associated ECM remodeling on smooth muscle function remain poorly defined.. Here, we imaged fibrillar ECM components across layers of healthy and hypertensive arteries in a large animal model and quantified their stiffness with submicron and piconewton resolution.

    Incubation:

    Article Title: Characterizing age-dependent diamagnetic and paramagnetic susceptibility properties in 5xFAD Alzheimer's mouse brain using high-resolution MRI.
    Article Snippet: Nuclei were counterstained with DAPI (62 248, ThermoFisher Scientific). .. Following secondary antibody incubation, sections were washed three times in 1×PBS containing 0.1% Triton X-100 and mounted on glass slides using Vectashield Antifade mounting medium (H-1000, Vector Laboratories) and sealed with CoverGrip Coverslip Sealant (23 005, Biotium, Fremont, CA). .. Fluorescence images were acquired using a Nikon Eclipse Ti inverted microscope equipped with Plan Fluor 10×/1.30 and 100×/1.49 objectives and NIS-Elements software (Nikon Instruments).

    Glass:

    Article Title: Characterizing age-dependent diamagnetic and paramagnetic susceptibility properties in 5xFAD Alzheimer's mouse brain using high-resolution MRI.
    Article Snippet: Nuclei were counterstained with DAPI (62 248, ThermoFisher Scientific). .. Following secondary antibody incubation, sections were washed three times in 1×PBS containing 0.1% Triton X-100 and mounted on glass slides using Vectashield Antifade mounting medium (H-1000, Vector Laboratories) and sealed with CoverGrip Coverslip Sealant (23 005, Biotium, Fremont, CA). .. Fluorescence images were acquired using a Nikon Eclipse Ti inverted microscope equipped with Plan Fluor 10×/1.30 and 100×/1.49 objectives and NIS-Elements software (Nikon Instruments).

    Cell Culture:

    Article Title: Biomanufacturing Vascular Matrix-Mimetic Hydrogels from Quantified Arterial Micromechanics and Microstructure.
    Article Snippet: The microstructural and micromechanical properties of the arterial extracellular matrix (ECM) are central to vascular cell behavior but are disrupted by disease and aging.. Elastin and fibrillar collagens provide essential cues, yet the effects of disease-associated ECM remodeling on smooth muscle function remain poorly defined.. Here, we imaged fibrillar ECM components across layers of healthy and hypertensive arteries in a large animal model and quantified their stiffness with submicron and piconewton resolution.



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