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dab substrate  (Vector Laboratories)


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    Structured Review

    Vector Laboratories dab substrate
    Dab Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1973 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+red+detection+kit/VECTOR+Red+Alkaline+Phosphatase+(Red+AP)+Substrate+Kit/pmc13070831-99-33-36
    Average 96 stars, based on 1973 article reviews
    dab substrate - by Bioz Stars, 2026-11
    96/100 stars

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    other:

    Article Title: The nuclear cap-binding complex safeguards stress-resistant protein synthesis and proliferation of stem cells
    Article Snippet: For NE differentiation, hESCs were cultured on Matrigel-coated plates in an hESC medium (DMEM/F12 supplemented with 15% knockout serum replacement, minimum essential medium nonessential amino acids and 0.1 mM 2-mercaptoethanol) added with 10 μM SB431542 (PeproTech) and 2 μM dorsomorphin (Tocris) via the dual SMAD inhibition method.

    ALP Assay:

    Article Title: The nuclear cap-binding complex safeguards stress-resistant protein synthesis and proliferation of stem cells
    Article Snippet: .. Reprogrammed iPSC colonies were fixed with 4% paraformaldehyde for 15 min and subjected to an alkaline phosphatase assay by using the Vector Red Alkaline Phosphatase Substrate Kit I (Vector laboratories). .. HEK293T cells were transfected with the indicated plasmids using either the calcium phosphate method or Lipofectamine 2000 (Invitrogen).

    Incubation:

    Article Title: A chlorhexidine-releasing epoxy-based coating on titanium implants prevents Staphylococcus aureus experimental biomaterial-associated infection
    Article Snippet: .. Next, sections were incubated with 1: 10000 diluted rabbit anti-S. aureus polyclonal IgG (20920, Abcam) for 1 h at RT, with undiluted BrightVision anti-rabbit AP for 30 min at RT and with Vector Red (SK-5100, Vector Labs) for 10 min at RT, again with three TBS washes between all incubations. .. After another HIER-citrate (10 min at 98 °C) and Superblock incubation, sections were incubated with 1: 1000 diluted rat anti-mouse Ly-6G-FITC (neutrophils; 553126, BD) in TBS for 2 h at RT, with 1: 1000 diluted rabbit anti-FITC (4510-7804, Nuclilab) in the presence of 5 % normal mouse serum in TBS for 15 min at RT, with undiluted BrightVision anti-rabbit IgG-HRP (DPVR110HRP, Immunologic) for 30 min at RT, and finally stained for ~8 min with DAB (BS04-110, Immunologic) until they turned yellow/brownish.

    Plasmid Preparation:

    Article Title: A chlorhexidine-releasing epoxy-based coating on titanium implants prevents Staphylococcus aureus experimental biomaterial-associated infection
    Article Snippet: .. Next, sections were incubated with 1: 10000 diluted rabbit anti-S. aureus polyclonal IgG (20920, Abcam) for 1 h at RT, with undiluted BrightVision anti-rabbit AP for 30 min at RT and with Vector Red (SK-5100, Vector Labs) for 10 min at RT, again with three TBS washes between all incubations. .. After another HIER-citrate (10 min at 98 °C) and Superblock incubation, sections were incubated with 1: 1000 diluted rat anti-mouse Ly-6G-FITC (neutrophils; 553126, BD) in TBS for 2 h at RT, with 1: 1000 diluted rabbit anti-FITC (4510-7804, Nuclilab) in the presence of 5 % normal mouse serum in TBS for 15 min at RT, with undiluted BrightVision anti-rabbit IgG-HRP (DPVR110HRP, Immunologic) for 30 min at RT, and finally stained for ~8 min with DAB (BS04-110, Immunologic) until they turned yellow/brownish.

    Transcranial Magnetic Stimulation:

    Article Title: A chlorhexidine-releasing epoxy-based coating on titanium implants prevents Staphylococcus aureus experimental biomaterial-associated infection
    Article Snippet: .. Next, sections were incubated with 1: 10000 diluted rabbit anti-S. aureus polyclonal IgG (20920, Abcam) for 1 h at RT, with undiluted BrightVision anti-rabbit AP for 30 min at RT and with Vector Red (SK-5100, Vector Labs) for 10 min at RT, again with three TBS washes between all incubations. .. After another HIER-citrate (10 min at 98 °C) and Superblock incubation, sections were incubated with 1: 1000 diluted rat anti-mouse Ly-6G-FITC (neutrophils; 553126, BD) in TBS for 2 h at RT, with 1: 1000 diluted rabbit anti-FITC (4510-7804, Nuclilab) in the presence of 5 % normal mouse serum in TBS for 15 min at RT, with undiluted BrightVision anti-rabbit IgG-HRP (DPVR110HRP, Immunologic) for 30 min at RT, and finally stained for ~8 min with DAB (BS04-110, Immunologic) until they turned yellow/brownish.



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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: BRCA1 and 53BP1 Regulate Reprogramming Efficiency by Mediating DNA Repair Pathway Choice at Replication-associated Double-Strand Breaks

    doi: 10.1016/j.celrep.2024.114006

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Cells were fixed on reprogramming day 18–20 and stained for alkaline phosphatase with the Vector Red detection kit (Vector Laboratories #SK-5100).

    Techniques: Purification, Transduction, Recombinant, Knock-Out, Plasmid Preparation, Software, Imaging