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immpact nova red chromogen substrate  (Vector Laboratories)


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    Structured Review

    Vector Laboratories immpact nova red chromogen substrate
    Immpact Nova Red Chromogen Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+red+chromogen+solution/ImmPACT+AEC+Peroxidase+(HRP)+Substrate/pmc05376500-67-5-9
    Average 95 stars, based on 114 article reviews
    immpact nova red chromogen substrate - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Immune Modulatory Oxysterols Produced from Cholesterol-Containing Lipid Nanoparticles Regulate Tumor Growth
    Article Snippet: Antibody against K i -67 (Cat. #12202, Cell Signaling) was diluted 1:400 with 1% BSA, 0.5% Triton X-100, and 1× PBS and incubated on tissue sections overnight at 4 °C. .. Endogenous peroxidase was blocked with 3% hydrogen peroxide for 30 min at room temperature, followed by biotin-conjugated secondary goat antirabbit antibody diluted 1:250 (Cat. #50-194-1796, Jackson Immuno Research Laboratories) in 0.05% Tween 20 and 1× PBS, VECTASTAIN ABC (Cat. #PK-4000, Vector Laboratories) and AEC (Cat. #SK-4205, Vector Laboratories) staining according to manufacturer instructions. .. Tissues were counterstained with hematoxylin and mounted with aqueous mounting media (Cat. #H-5501, Vector Laboratories).

    Article Title: Immune Modulatory Oxysterols Produced from Cholesterol-Containing Lipid Nanoparticles Regulate Tumor Growth.
    Article Snippet: Antibody against Ki-67 (Cat. #12202, Cell Signaling) was diluted 1:400 with 1% BSA, 0.5% Triton X-100, and 1× PBS and incubated on tissue sections overnight at 4 °C. .. Endogenous peroxidase was blocked with 3% hydrogen peroxide for 30 min at room temperature, followed by biotin-conjugated secondary goat antirabbit antibody diluted 1:250 (Cat. #50-194-1796, Jackson Immuno Research Laboratories) in 0.05% Tween 20 and 1× PBS, VECTASTAIN ABC (Cat. #PK-4000, Vector Laboratories) and AEC (Cat. #SK-4205, Vector Laboratories) staining according to manufacturer instructions. .. Tissues were counterstained with hematoxylin and mounted with aqueous mounting media (Cat. #H-5501, Vector Laboratories).



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    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
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    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
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    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
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    Vector Laboratories vector red chromogen solution
    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by <t>chromogenic</t> <t>alkaline</t> <t>phosphatase</t> (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).
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    Average 96 stars, based on 1 article reviews
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    Image Search Results


    Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Journal: Theranostics

    Article Title: Superior Performance of Aptamer in Tumor Penetration over Antibody: Implication of Aptamer-Based Theranostics in Solid Tumors

    doi: 10.7150/thno.11711

    Figure Lengend Snippet: Time-dependent penetration of PEGylated aptamer and antibody in relation to blood vessels in HT29 xenograft tumors. (a) Representative images of double staining of aptamer or antibody and blood vessels in tumor sections dissected from treated mice-bearing HT29 xenografts 3 h and 24 h after i.v. administration of aptamer or antibody at a dose of 2 nmol/mouse. Blood vessels were stained by chromogenic alkaline phosphatase (black arrow); while aptamer or antibody were stained using DAB peroxidase substrate (brown). Scale bar: 200 μm. (b-c) Quantitative determination of staining intensity against a given perpendicular distance (20-200 μm) to the blood vessels at 3 h. (b) and 24 h (c) after i.v. injection of aptamer or antibody. Data are means ± SEM (n=8).

    Article Snippet: The chromogenic alkaline phosphatase solution (Vector Labs, Cat #SK-5100) was prepared according to the manufacturer's instruction and added to the slides following 1 h staining of secondary anti-biotin antibody (Vector Labs, Cat #SP-3020) (1: 250 dilution).

    Techniques: Double Staining, Staining, Injection