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p unc 122 gfp c elegans expression vector  (Addgene inc)


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    Structured Review

    Addgene inc p unc 122 gfp c elegans expression vector
    KEY RESOURCES TABLE
    P Unc 122 Gfp C Elegans Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+punc/pCFJ68+-+Exp5605_Punc-122%3A%3AGFP%3A%3Aunc-54utr+(Plasmid+%2319325)/pmc06733259-85-0-8
    Average 90 stars, based on 9 article reviews
    p unc 122 gfp c elegans expression vector - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "The long non-coding RNA lep-5 promotes the juvenile-to-adult transition by destabilizing LIN-28"

    Article Title: The long non-coding RNA lep-5 promotes the juvenile-to-adult transition by destabilizing LIN-28

    Journal: Developmental cell

    doi: 10.1016/j.devcel.2019.03.003

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Mutagenesis, Labeling, SYBR Green Assay, Binding Assay, Plasmid Preparation, Expressing, Clone Assay, Software, Sequencing

    Related Articles

    Plasmid Preparation:

    Article Title: Wnt Secretion Is Regulated by the Tetraspan Protein HIC-1 through Its Interaction with Neurabin/NAB-1
    Article Snippet: pCFJ910 , Addgene , Plasmid#44481 (Erik Jorgensen lab). .. pCFJ68 (Exp5605_Punc-122::GFP::unc-54utr ) , Addgene , Plasmid#19325 (Erik Jorgensen lab). .. pCFJ90 (Pmyo-2::mCherry::unc-54utr ) , Addgene , Plasmid#19327 (Erik Jorgensen lab).

    Article Title: A neuropeptide signal confers ethanol state dependency during olfactory learning in Caenorhabditis elegans
    Article Snippet: .. The resulting Pgcy5::GtACR1::tdTomato expression plasmid was injected into N2 worms at 10 ng/μL with 10 ng/μL pCFJ68 (Addgene plasmid 19325) coinjection marker and 75 ng/μL 1 kb DNA ladder (New England Biolabs) to create the extrachromosomal array-bearing strain JCB350: betEx14 [Pgcy5::GtACR1::tdTomato]. ..

    Article Title: A high-throughput method to deliver targeted optogenetic stimulation to moving C . elegans populations
    Article Snippet: .. Specifically, to generate AML470, a plasmid mix containing 10 ng/ul of pAL::pmec-4::Chrimson::SL2::mCherry::unc-54 (RRID:Addgene_107745) and 100 ng/ul of pCFJ68 unc-122::GFP (RRID:Addgene_19325) were injected into CZ20310 [ ] and then integrated via UV irradiation. ..

    Article Title: The long non-coding RNA lep-5 promotes the juvenile-to-adult transition by destabilizing LIN-28
    Article Snippet: .. P unc-122 ::GFP C. elegans expression vector , Addgene , RRID:Addgene_19325. .. GFP::NLS C. elegans expression vector , Addgene , RRID:Addgene_1622.

    Article Title: [2109.05303] Closed-loop targeted optogenetic stimulation of C. elegans populations
    Article Snippet: .. Strain RRID Genotype Notes Ref AML67 RRID:WB- STRAIN:WBStrain00000193 wtfIs46[pmec-4::Chrimson::SL2::mCherry::unc54 40ng/ul] 40 ng Chrimson injection [48] AML470 juSi164 unc-119(ed3) III; wtfIs458 [mec4::Chrimson4.2::SL2::mCherry::unc-54 10 ng/ul + unc-122::GFP 100 ng/ul] 10 ng Chrimson injection This work Specifically, to generate AML470, a plasmid mix containing 10 ng/ul of pAL::pmec-4::Chrimson::SL2::mCherry::unc-54 (RRID:Addgene 107745) and 100 ng/ul of pCFJ68 unc-122::GFP (RRID:Addgene 19325) were injected into CZ20310 [72] and then integrated via UV irradiation. ..

    Expressing:

    Article Title: A neuropeptide signal confers ethanol state dependency during olfactory learning in Caenorhabditis elegans
    Article Snippet: .. The resulting Pgcy5::GtACR1::tdTomato expression plasmid was injected into N2 worms at 10 ng/μL with 10 ng/μL pCFJ68 (Addgene plasmid 19325) coinjection marker and 75 ng/μL 1 kb DNA ladder (New England Biolabs) to create the extrachromosomal array-bearing strain JCB350: betEx14 [Pgcy5::GtACR1::tdTomato]. ..

    Article Title: The long non-coding RNA lep-5 promotes the juvenile-to-adult transition by destabilizing LIN-28
    Article Snippet: .. P unc-122 ::GFP C. elegans expression vector , Addgene , RRID:Addgene_19325. .. GFP::NLS C. elegans expression vector , Addgene , RRID:Addgene_1622.

    Injection:

    Article Title: A neuropeptide signal confers ethanol state dependency during olfactory learning in Caenorhabditis elegans
    Article Snippet: .. The resulting Pgcy5::GtACR1::tdTomato expression plasmid was injected into N2 worms at 10 ng/μL with 10 ng/μL pCFJ68 (Addgene plasmid 19325) coinjection marker and 75 ng/μL 1 kb DNA ladder (New England Biolabs) to create the extrachromosomal array-bearing strain JCB350: betEx14 [Pgcy5::GtACR1::tdTomato]. ..

    Article Title: A high-throughput method to deliver targeted optogenetic stimulation to moving C . elegans populations
    Article Snippet: .. Specifically, to generate AML470, a plasmid mix containing 10 ng/ul of pAL::pmec-4::Chrimson::SL2::mCherry::unc-54 (RRID:Addgene_107745) and 100 ng/ul of pCFJ68 unc-122::GFP (RRID:Addgene_19325) were injected into CZ20310 [ ] and then integrated via UV irradiation. ..

    Article Title: [2109.05303] Closed-loop targeted optogenetic stimulation of C. elegans populations
    Article Snippet: .. Strain RRID Genotype Notes Ref AML67 RRID:WB- STRAIN:WBStrain00000193 wtfIs46[pmec-4::Chrimson::SL2::mCherry::unc54 40ng/ul] 40 ng Chrimson injection [48] AML470 juSi164 unc-119(ed3) III; wtfIs458 [mec4::Chrimson4.2::SL2::mCherry::unc-54 10 ng/ul + unc-122::GFP 100 ng/ul] 10 ng Chrimson injection This work Specifically, to generate AML470, a plasmid mix containing 10 ng/ul of pAL::pmec-4::Chrimson::SL2::mCherry::unc-54 (RRID:Addgene 107745) and 100 ng/ul of pCFJ68 unc-122::GFP (RRID:Addgene 19325) were injected into CZ20310 [72] and then integrated via UV irradiation. ..

    Marker:

    Article Title: A neuropeptide signal confers ethanol state dependency during olfactory learning in Caenorhabditis elegans
    Article Snippet: .. The resulting Pgcy5::GtACR1::tdTomato expression plasmid was injected into N2 worms at 10 ng/μL with 10 ng/μL pCFJ68 (Addgene plasmid 19325) coinjection marker and 75 ng/μL 1 kb DNA ladder (New England Biolabs) to create the extrachromosomal array-bearing strain JCB350: betEx14 [Pgcy5::GtACR1::tdTomato]. ..

    Irradiation:

    Article Title: A high-throughput method to deliver targeted optogenetic stimulation to moving C . elegans populations
    Article Snippet: .. Specifically, to generate AML470, a plasmid mix containing 10 ng/ul of pAL::pmec-4::Chrimson::SL2::mCherry::unc-54 (RRID:Addgene_107745) and 100 ng/ul of pCFJ68 unc-122::GFP (RRID:Addgene_19325) were injected into CZ20310 [ ] and then integrated via UV irradiation. ..

    Article Title: [2109.05303] Closed-loop targeted optogenetic stimulation of C. elegans populations
    Article Snippet: .. Strain RRID Genotype Notes Ref AML67 RRID:WB- STRAIN:WBStrain00000193 wtfIs46[pmec-4::Chrimson::SL2::mCherry::unc54 40ng/ul] 40 ng Chrimson injection [48] AML470 juSi164 unc-119(ed3) III; wtfIs458 [mec4::Chrimson4.2::SL2::mCherry::unc-54 10 ng/ul + unc-122::GFP 100 ng/ul] 10 ng Chrimson injection This work Specifically, to generate AML470, a plasmid mix containing 10 ng/ul of pAL::pmec-4::Chrimson::SL2::mCherry::unc-54 (RRID:Addgene 107745) and 100 ng/ul of pCFJ68 unc-122::GFP (RRID:Addgene 19325) were injected into CZ20310 [72] and then integrated via UV irradiation. ..

    C.Elegans:

    Article Title: The long non-coding RNA lep-5 promotes the juvenile-to-adult transition by destabilizing LIN-28
    Article Snippet: .. P unc-122 ::GFP C. elegans expression vector , Addgene , RRID:Addgene_19325. .. GFP::NLS C. elegans expression vector , Addgene , RRID:Addgene_1622.



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    https://www.bioz.com/product/vector+punc/pCFJ68+-+Exp5605_Punc-122%3A%3AGFP%3A%3Aunc-54utr+(Plasmid+%2319325)/pmc06733259-85-0-8
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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Developmental cell

    Article Title: The long non-coding RNA lep-5 promotes the juvenile-to-adult transition by destabilizing LIN-28

    doi: 10.1016/j.devcel.2019.03.003

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: P unc-122 ::GFP C. elegans expression vector , Addgene , RRID:Addgene_19325.

    Techniques: Recombinant, Mutagenesis, Labeling, SYBR Green Assay, Binding Assay, Plasmid Preparation, Expressing, Clone Assay, Software, Sequencing