Review



blunt end cloning vector pjet1 2  (Addgene inc)


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    Structured Review

    Addgene inc blunt end cloning vector pjet1 2
    Blunt End Cloning Vector Pjet1 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+plasmid/NHR1-Flag+pcDNA3%2E1+(Plasmid+%2317315)/pmc12804166-56-8-12
    Average 94 stars, based on 2 article reviews
    blunt end cloning vector pjet1 2 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
    Article Snippet: This sequence, along with the gRNA scaffold from p426-SNR52p-gRNA (Addgene number: #43803) and the B. cinerea U6 promoter, was assembled into a PU6-20 nt-gRNA scaffold construct. .. Finally, the resulting fragment was cloned into the blunt-end cloning vector pJET1.2 (Addgene number: #173156) for amplification. ..

    Article Title: Evolution of inwardly rectifying potassium channels and their gene expression in zebrafish embryos.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense zebrafish kir gene clones are available through Addgene (Plasmid # 173125-173151). .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense zebrafish kir gene clones are available through Addgene (Plasmid # 173125-173151). .. DNA templates were purified with the NucleoSpin Gel and PCR Clean-up Kit (Takara Bio) before in vitro transcription.

    Cloning:

    Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
    Article Snippet: This sequence, along with the gRNA scaffold from p426-SNR52p-gRNA (Addgene number: #43803) and the B. cinerea U6 promoter, was assembled into a PU6-20 nt-gRNA scaffold construct. .. Finally, the resulting fragment was cloned into the blunt-end cloning vector pJET1.2 (Addgene number: #173156) for amplification. ..

    Plasmid Preparation:

    Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
    Article Snippet: This sequence, along with the gRNA scaffold from p426-SNR52p-gRNA (Addgene number: #43803) and the B. cinerea U6 promoter, was assembled into a PU6-20 nt-gRNA scaffold construct. .. Finally, the resulting fragment was cloned into the blunt-end cloning vector pJET1.2 (Addgene number: #173156) for amplification. ..

    Article Title: Evolution of inwardly rectifying potassium channels and their gene expression in zebrafish embryos.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense zebrafish kir gene clones are available through Addgene (Plasmid # 173125-173151). .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense zebrafish kir gene clones are available through Addgene (Plasmid # 173125-173151). .. DNA templates were purified with the NucleoSpin Gel and PCR Clean-up Kit (Takara Bio) before in vitro transcription.

    Amplification:

    Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
    Article Snippet: This sequence, along with the gRNA scaffold from p426-SNR52p-gRNA (Addgene number: #43803) and the B. cinerea U6 promoter, was assembled into a PU6-20 nt-gRNA scaffold construct. .. Finally, the resulting fragment was cloned into the blunt-end cloning vector pJET1.2 (Addgene number: #173156) for amplification. ..



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