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vector anep8 cas9  (Addgene inc)


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    Structured Review

    Addgene inc vector anep8 cas9
    Vector Anep8 Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+plasmid/Anep8_Cas9+(Plasmid+%23117169)/pmc12804166-53-34-36
    Average 94 stars, based on 2 article reviews
    vector anep8 cas9 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
    Article Snippet: .. The fragment Cas9-4NLSs was amplified with primers PpkiA - Cas9 -F and Cas9 - TglaA -R using pET28a- Cas9 - 4NLSs as template, and cloned into the Nhe I and Pac I sites of vector Anep8-Cas9 (Addgene number: #117169) to generate pAnep8- Cas9 - 4NLSs - AMA1 . ..

    Clone Assay:

    Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
    Article Snippet: .. The fragment Cas9-4NLSs was amplified with primers PpkiA - Cas9 -F and Cas9 - TglaA -R using pET28a- Cas9 - 4NLSs as template, and cloned into the Nhe I and Pac I sites of vector Anep8-Cas9 (Addgene number: #117169) to generate pAnep8- Cas9 - 4NLSs - AMA1 . ..

    Plasmid Preparation:

    Article Title: Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
    Article Snippet: .. The fragment Cas9-4NLSs was amplified with primers PpkiA - Cas9 -F and Cas9 - TglaA -R using pET28a- Cas9 - 4NLSs as template, and cloned into the Nhe I and Pac I sites of vector Anep8-Cas9 (Addgene number: #117169) to generate pAnep8- Cas9 - 4NLSs - AMA1 . ..

    Article Title: Non-homologous end-joining-deficient filamentous fungal strains mitigate the impact of off-target mutations during the application of CRISPR/Cas9
    Article Snippet: Escherichia coli DH5α was used to propagate plasmids, and was grown in Luria-Bertani medium containing 50 μg/mL of ampicillin (Sigma-Aldrich). .. The self-replicative CRISPR/Cas9 plasmid ANEp8-Cas9- pyrG was used (AddGene #117169) ( ). .. The self-replicative CRISPR/Cas9 plasmid ANEp8-Cas9- pyrG was used (AddGene #117169) ( ).

    Article Title: Non-homologous end-joining-deficient filamentous fungal strains mitigate the impact of off-target mutations during the application of CRISPR/Cas9.
    Article Snippet: .. The self-replicative CRISPR/Cas9 plasmid ANEp8-Cas9-pyrG was used (AddGene #117169) (38). ..

    CRISPR:

    Article Title: Non-homologous end-joining-deficient filamentous fungal strains mitigate the impact of off-target mutations during the application of CRISPR/Cas9
    Article Snippet: Escherichia coli DH5α was used to propagate plasmids, and was grown in Luria-Bertani medium containing 50 μg/mL of ampicillin (Sigma-Aldrich). .. The self-replicative CRISPR/Cas9 plasmid ANEp8-Cas9- pyrG was used (AddGene #117169) ( ). .. The self-replicative CRISPR/Cas9 plasmid ANEp8-Cas9- pyrG was used (AddGene #117169) ( ).

    Article Title: Non-homologous end-joining-deficient filamentous fungal strains mitigate the impact of off-target mutations during the application of CRISPR/Cas9.
    Article Snippet: .. The self-replicative CRISPR/Cas9 plasmid ANEp8-Cas9-pyrG was used (AddGene #117169) (38). ..



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