pgbkt-7 vector (Becton Dickinson)
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Pgbkt 7 Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pgbkt7/pgbkt+7+vector/pm21393573-34-14-16
Average 90 stars, based on 1 article reviews
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Clone Assay:Article Title: VSTM2L is a novel secreted antagonist of the neuroprotective peptide Humanin. Article Snippet: Humanin (HN) is a 24-residue peptide displaying a protective activity in vitro against a range of cytotoxic and neurotoxic insults, as well as mediating in vivo amelioration of Alzheimer disease (AD)-related memory impairment in experimental models.. Published evidence suggests that the mechanisms through which HN exerts its cytoand neuroprotective activity may include its secretion and binding to membraneassociated receptors.. Here, we describe the identification of a new modulator of HN neuroprotective activity, V-set and transmembrane domain containing 2 like (VSTM2L), previously known as C20orf102. Plasmid Preparation:Article Title: VSTM2L is a novel secreted antagonist of the neuroprotective peptide Humanin. Article Snippet: Humanin (HN) is a 24-residue peptide displaying a protective activity in vitro against a range of cytotoxic and neurotoxic insults, as well as mediating in vivo amelioration of Alzheimer disease (AD)-related memory impairment in experimental models.. Published evidence suggests that the mechanisms through which HN exerts its cytoand neuroprotective activity may include its secretion and binding to membraneassociated receptors.. Here, we describe the identification of a new modulator of HN neuroprotective activity, V-set and transmembrane domain containing 2 like (VSTM2L), previously known as C20orf102. |
![Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a <t>GAL4‐activating</t> domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8646/pmc12948646/pmc12948646__MPP-27-e70202-g007.jpg)