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clonexpress ii one step cloning kit  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co clonexpress ii one step cloning kit
    Clonexpress Ii One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 6344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+pgbkt7/ClonExpress+II+One+Step+Cloning+Kit/custom%40c112%4039985078
    Average 99 stars, based on 6344 article reviews
    clonexpress ii one step cloning kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Functional Validation of a CC2D2A Non-Canonical Splice-Site Variant Supports Prenatal Molecular Testing in Joubert Syndrome.
    Article Snippet: .. The minigene regions spanning CC2D2A exons 6–8 and flanking introns were cloned into the pMini-CopGFP vector (Beijing Hitrobio Biotechnology, Beijing, China) using the ClonExpress II One Step Cloning Kit (Cat. No. C112-02, Vazyme, Nanjing, China). ..

    Article Title: Targeting oncogenic SOX2 condensates suppresses squamous cell carcinoma progression.
    Article Snippet: .. SOX2, HSP70, their mutants, and hnRNPA IDR fusion fragments were cloned into pLenti-EF1α-IRES-blast vectors with EGFP, mCherry, FLAG, or Myc tags using a one-step cloning kit (Vazyme, C112). ..

    Plasmid Preparation:

    Article Title: Functional Validation of a CC2D2A Non-Canonical Splice-Site Variant Supports Prenatal Molecular Testing in Joubert Syndrome.
    Article Snippet: .. The minigene regions spanning CC2D2A exons 6–8 and flanking introns were cloned into the pMini-CopGFP vector (Beijing Hitrobio Biotechnology, Beijing, China) using the ClonExpress II One Step Cloning Kit (Cat. No. C112-02, Vazyme, Nanjing, China). ..

    Article Title: Antagonism of PRDX1 and Japanese encephalitis virus via the autophagy pathway.
    Article Snippet: .. A ClonExpress II One-Step Cloning Kit (C112-02, Vazyme Biotech) was used to clone target fragments such as PRDX1 into the p3 × Flag-CMV-7.1 vector. .. After the cells reached 80%–90% confluence, the plasmids were transfected with Lipofectamine 3000 Month XXXX Volume 0 Issue 0 10.1128/jvi.00404-2612 h ttp s: //j ou rn al s. as m .o rg /jo ur na l/j vi o n 24 O ct ob er 2 02 6 by 2 40 9: 40 d2 :4 c: 89 7f :6 cb c: aa 98 :4 74 5: 89 49 . reagent (L300015, Invitrogen)

    Article Title: Self-extending DNA recording with stochastic and programmed writing
    Article Snippet: .. For the derived inducible PE construct, the PEmax–P2A–hMLH1dn fragment was PCR-amplified from the donor plasmid and joined to an inverse-PCR-linearized XLone-PE2 backbone by homology-based assembly using the ClonExpress II One Step Cloning Kit (Vazyme, C112-01). ..

    Article Title: Thioredoxin‐2 Alleviates Osteoporosis by Restraining FOXO3a Nuclear Translocation
    Article Snippet: DNA sequences encoding the wild‐type genes were purchased from GenScript, amplified by polymerase chain reaction (PCR), and inserted into different vectors. .. All PCR‐amplified fragments and linearized vector backbones with homologous ends were seamlessly assembled using the ClonExpress II One Step Cloning Kit (Vazyme, C112‐01) according to the manufacturer's instructions. ..

    Article Title: Fasciola gigantica Recombinant Bone Morphogenetic Protein ( Fg BMP) Modulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells.
    Article Snippet: .. Then, the PCR product was ligated into the pET-28a(+) vector and transferred into Escherichia coli (DH5α) following the instructions of the ClonExpress II One Step Cloning Kit (C112-01, Vazyme, Nanjing, China). ..

    Cloning:

    Article Title: Functional Validation of a CC2D2A Non-Canonical Splice-Site Variant Supports Prenatal Molecular Testing in Joubert Syndrome.
    Article Snippet: .. The minigene regions spanning CC2D2A exons 6–8 and flanking introns were cloned into the pMini-CopGFP vector (Beijing Hitrobio Biotechnology, Beijing, China) using the ClonExpress II One Step Cloning Kit (Cat. No. C112-02, Vazyme, Nanjing, China). ..

    Article Title: Targeting oncogenic SOX2 condensates suppresses squamous cell carcinoma progression.
    Article Snippet: .. SOX2, HSP70, their mutants, and hnRNPA IDR fusion fragments were cloned into pLenti-EF1α-IRES-blast vectors with EGFP, mCherry, FLAG, or Myc tags using a one-step cloning kit (Vazyme, C112). ..

    Article Title: Two-step bioconversion of pyridoxal 5′-phosphate from pyridoxine through cofactor regeneration and pyridoxine 5′-phosphate oxidase evolution
    Article Snippet: .. The purified DNA fragments were subsequently inserted into the pYB1s via a ClonExpress II One Step Cloning Kit (Vazyme, Nanjing, China) with the primers P1–P52 ( ). ..

    Article Title: Antagonism of PRDX1 and Japanese encephalitis virus via the autophagy pathway.
    Article Snippet: .. A ClonExpress II One-Step Cloning Kit (C112-02, Vazyme Biotech) was used to clone target fragments such as PRDX1 into the p3 × Flag-CMV-7.1 vector. .. After the cells reached 80%–90% confluence, the plasmids were transfected with Lipofectamine 3000 Month XXXX Volume 0 Issue 0 10.1128/jvi.00404-2612 h ttp s: //j ou rn al s. as m .o rg /jo ur na l/j vi o n 24 O ct ob er 2 02 6 by 2 40 9: 40 d2 :4 c: 89 7f :6 cb c: aa 98 :4 74 5: 89 49 . reagent (L300015, Invitrogen)

    Article Title: Self-extending DNA recording with stochastic and programmed writing
    Article Snippet: .. For the derived inducible PE construct, the PEmax–P2A–hMLH1dn fragment was PCR-amplified from the donor plasmid and joined to an inverse-PCR-linearized XLone-PE2 backbone by homology-based assembly using the ClonExpress II One Step Cloning Kit (Vazyme, C112-01). ..

    Article Title: Thioredoxin‐2 Alleviates Osteoporosis by Restraining FOXO3a Nuclear Translocation
    Article Snippet: DNA sequences encoding the wild‐type genes were purchased from GenScript, amplified by polymerase chain reaction (PCR), and inserted into different vectors. .. All PCR‐amplified fragments and linearized vector backbones with homologous ends were seamlessly assembled using the ClonExpress II One Step Cloning Kit (Vazyme, C112‐01) according to the manufacturer's instructions. ..

    Article Title: WHIRLY1 Interactome Profiling Reveals the Chloroplast Fate Correlation with Stromule-like Morphologies in Arabidopsis Leaves Under Dark and DBMIB Stress Conditions.
    Article Snippet: .. All constructs were generated according to the manual of the In-Fusion cloning kit (Clon-Express II One Step Cloning kit, Vazyme, Nanjing, China, C112-01). ..

    Article Title: Fasciola gigantica Recombinant Bone Morphogenetic Protein ( Fg BMP) Modulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells.
    Article Snippet: .. Then, the PCR product was ligated into the pET-28a(+) vector and transferred into Escherichia coli (DH5α) following the instructions of the ClonExpress II One Step Cloning Kit (C112-01, Vazyme, Nanjing, China). ..

    Purification:

    Article Title: Two-step bioconversion of pyridoxal 5′-phosphate from pyridoxine through cofactor regeneration and pyridoxine 5′-phosphate oxidase evolution
    Article Snippet: .. The purified DNA fragments were subsequently inserted into the pYB1s via a ClonExpress II One Step Cloning Kit (Vazyme, Nanjing, China) with the primers P1–P52 ( ). ..

    Derivative Assay:

    Article Title: Self-extending DNA recording with stochastic and programmed writing
    Article Snippet: .. For the derived inducible PE construct, the PEmax–P2A–hMLH1dn fragment was PCR-amplified from the donor plasmid and joined to an inverse-PCR-linearized XLone-PE2 backbone by homology-based assembly using the ClonExpress II One Step Cloning Kit (Vazyme, C112-01). ..

    Construct:

    Article Title: Self-extending DNA recording with stochastic and programmed writing
    Article Snippet: .. For the derived inducible PE construct, the PEmax–P2A–hMLH1dn fragment was PCR-amplified from the donor plasmid and joined to an inverse-PCR-linearized XLone-PE2 backbone by homology-based assembly using the ClonExpress II One Step Cloning Kit (Vazyme, C112-01). ..

    Article Title: WHIRLY1 Interactome Profiling Reveals the Chloroplast Fate Correlation with Stromule-like Morphologies in Arabidopsis Leaves Under Dark and DBMIB Stress Conditions.
    Article Snippet: .. All constructs were generated according to the manual of the In-Fusion cloning kit (Clon-Express II One Step Cloning kit, Vazyme, Nanjing, China, C112-01). ..

    Polymerase Chain Reaction:

    Article Title: Self-extending DNA recording with stochastic and programmed writing
    Article Snippet: .. For the derived inducible PE construct, the PEmax–P2A–hMLH1dn fragment was PCR-amplified from the donor plasmid and joined to an inverse-PCR-linearized XLone-PE2 backbone by homology-based assembly using the ClonExpress II One Step Cloning Kit (Vazyme, C112-01). ..

    Article Title: Thioredoxin‐2 Alleviates Osteoporosis by Restraining FOXO3a Nuclear Translocation
    Article Snippet: DNA sequences encoding the wild‐type genes were purchased from GenScript, amplified by polymerase chain reaction (PCR), and inserted into different vectors. .. All PCR‐amplified fragments and linearized vector backbones with homologous ends were seamlessly assembled using the ClonExpress II One Step Cloning Kit (Vazyme, C112‐01) according to the manufacturer's instructions. ..

    Article Title: Fasciola gigantica Recombinant Bone Morphogenetic Protein ( Fg BMP) Modulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells.
    Article Snippet: .. Then, the PCR product was ligated into the pET-28a(+) vector and transferred into Escherichia coli (DH5α) following the instructions of the ClonExpress II One Step Cloning Kit (C112-01, Vazyme, Nanjing, China). ..

    Generated:

    Article Title: WHIRLY1 Interactome Profiling Reveals the Chloroplast Fate Correlation with Stromule-like Morphologies in Arabidopsis Leaves Under Dark and DBMIB Stress Conditions.
    Article Snippet: .. All constructs were generated according to the manual of the In-Fusion cloning kit (Clon-Express II One Step Cloning kit, Vazyme, Nanjing, China, C112-01). ..



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