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H-1200  (Vector Laboratories)


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    Structured Review

    Vector Laboratories H-1200
    H 1200, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 22145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectashield-dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pmc13127484-31-0-7
    Average 98 stars, based on 22145 article reviews
    H-1200 - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Integrated Reproductive and Cytogenetic Assessment of Infertility in a Captive Jaguar ( Panthera onca )
    Article Snippet: .. Specifically, the slides were aged at 65°C for 1 h, immersed in 0.4% HCl for 20 min, treated with 5% Ba(OH) 2 solution for 3 min at 45°C and incubated in 2× SSC for 1 h at 65°C, then rinsed in distilled water and counterstained with Vectashield DAPI anti‐fade medium (Vector Laboratories, Burlingame, CA, USA). .. G‐banding was performed sensu Seabright ( ).

    Article Title: Interaction of lifeguard (LFG/FAIM2) with phosphoinositides is associated with altered intracellular calcium homeostasis in breast cancer cells.
    Article Snippet: MDA-MB321 cells grown on glass slides in 4% paraformaldehyde for 20 min and permeabilized with 0.1% Triton X-100 (Sigma Aldrich, Steinheim, Germany) for 4 min. .. The slides were then incubated with rabbit polyclonal anti-hLFG antibody (1:100 dilution) and mouse polyclonal anti-h-ItdIns3,4,5P3 antibody (Invitrogen, Carlsbad, CA, USA) at 37◦C for 1 h. Slides were then washed three times with ice-cold PBS and incubated with Alexa Fluor 488- and Alexa Fluor 680-conjugated goat anti-rabbit and anti-mouse secondary antibodies (1:600 dilution each; Invitrogen, USA) at 37◦C for 30 min. After three additional washes with PBS, the samples were dried and mounted with Vectashield mounting medium (Vector Laboratories, Burlingame, CA) containing the DNA staining dye DAPI (4′,6′-diamidino-2-phenylindole). .. Images were captured using a ZEISS Axiovert 200 M fluorescence microscope equipped with the appropriate barrier filters.

    Automated:

    Article Title: Parvalbumin cell state dynamics regulate gamma oscillations and are disrupted in Alzheimer's disease.
    Article Snippet: Sections were then developed using diaminobenzidine (DAB; Vector Laboratories) for 6 minutes to enable chromogenic detection. .. Sections were mounted on slides, cover slipped with Vectashield antifade mounting medium with DAPI (Vector Laboratories, H-1200) or Cytoseal, and imaged with a Keyence BZ-X710 automated microscope. .. Quantification of amyloid deposits: Quantitative analysis of immunolabeled sections was conducted using ImageJ (NIH).

    Microscopy:

    Article Title: Parvalbumin cell state dynamics regulate gamma oscillations and are disrupted in Alzheimer's disease.
    Article Snippet: Sections were then developed using diaminobenzidine (DAB; Vector Laboratories) for 6 minutes to enable chromogenic detection. .. Sections were mounted on slides, cover slipped with Vectashield antifade mounting medium with DAPI (Vector Laboratories, H-1200) or Cytoseal, and imaged with a Keyence BZ-X710 automated microscope. .. Quantification of amyloid deposits: Quantitative analysis of immunolabeled sections was conducted using ImageJ (NIH).

    Glass:

    Article Title: Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells
    Article Snippet: Cells were further blocked with 1% BSA and 0.05% Tween 20 in PBS for 30 minutes, incubated with primary antibodies diluted with the blocking solution for overnight at 4°C, then the secondary antibodies Alexa Fluor 488, 594 and 633 (for flow cytometry sorting) (Invitrogen, A-11008, A-11032, A-21052) at 1:1000 in PBS for 1 hour at room temperature. .. Cells were counterstained for Nuclei by directly mounting coverslips on glass slides with VECTASHIELD Antifade Mounting Media (VECTOR LABORATORY) which contains DAPI. .. The images of stained cells are acquired by Zeiss LSM 780 UV confocal microscope.

    Staining:

    Article Title: Interaction of lifeguard (LFG/FAIM2) with phosphoinositides is associated with altered intracellular calcium homeostasis in breast cancer cells.
    Article Snippet: MDA-MB321 cells grown on glass slides in 4% paraformaldehyde for 20 min and permeabilized with 0.1% Triton X-100 (Sigma Aldrich, Steinheim, Germany) for 4 min. .. The slides were then incubated with rabbit polyclonal anti-hLFG antibody (1:100 dilution) and mouse polyclonal anti-h-ItdIns3,4,5P3 antibody (Invitrogen, Carlsbad, CA, USA) at 37◦C for 1 h. Slides were then washed three times with ice-cold PBS and incubated with Alexa Fluor 488- and Alexa Fluor 680-conjugated goat anti-rabbit and anti-mouse secondary antibodies (1:600 dilution each; Invitrogen, USA) at 37◦C for 30 min. After three additional washes with PBS, the samples were dried and mounted with Vectashield mounting medium (Vector Laboratories, Burlingame, CA) containing the DNA staining dye DAPI (4′,6′-diamidino-2-phenylindole). .. Images were captured using a ZEISS Axiovert 200 M fluorescence microscope equipped with the appropriate barrier filters.



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    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei <t>(DAPI;</t> blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .
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    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei <t>(DAPI;</t> blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .
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    Image Search Results


    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei (DAPI; blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei (DAPI; blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Morphology, Suspension, Microscopy, Scanning Electron Microscopy, Transmission Assay, Electron Microscopy, Brightfield, Analysis, Confocal, Staining

    Fatty acid transport in 3D enteroids in suspension (A) Representative confocal images following staining with DAPI (nuclei; blue), SLC27A4 (solute carrier family 27 member 4, green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the fatty acid transport protein in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B and C) AO and BO enteroids were incubated with fluorescent fatty acid analog C1-BODIPY-C12 for 10 min and imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. (B) C1-BODIPY-C12 accumulated in fluorescent lipid droplets in AO enteroids. Scale bars, 10 μm. (C) BO enteroids showed intraluminal accumulation of C1-BODIPY-C12. Scale bars, 20 μm. (D) AO enteroids (donor Hu18-9jej) were co-incubated with fluorescent fatty acid analog C1-BODIPY-C12 and LipidTOX-DR for 10 minto visualize lipid droplets. AO enteroids were imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. Scale bars, 20 μm; inset scale bars, 10 μm. (A–D) Enteroids from donor Hu18-9jej were used. See also .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: Fatty acid transport in 3D enteroids in suspension (A) Representative confocal images following staining with DAPI (nuclei; blue), SLC27A4 (solute carrier family 27 member 4, green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the fatty acid transport protein in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B and C) AO and BO enteroids were incubated with fluorescent fatty acid analog C1-BODIPY-C12 for 10 min and imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. (B) C1-BODIPY-C12 accumulated in fluorescent lipid droplets in AO enteroids. Scale bars, 10 μm. (C) BO enteroids showed intraluminal accumulation of C1-BODIPY-C12. Scale bars, 20 μm. (D) AO enteroids (donor Hu18-9jej) were co-incubated with fluorescent fatty acid analog C1-BODIPY-C12 and LipidTOX-DR for 10 minto visualize lipid droplets. AO enteroids were imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. Scale bars, 20 μm; inset scale bars, 10 μm. (A–D) Enteroids from donor Hu18-9jej were used. See also .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Suspension, Confocal, Staining, Expressing, Incubation, Widefield, Fluorescence, Microscopy

    BCRP expression, localization and activity in AO enteroids in suspension (A) Schematic illustrating BCRP efflux under physiological function, inhibition or absence (e.g., knockout). (B) Representative confocal images following staining with DAPI (nuclei; blue), BCRP (green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the BCRP transporter in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (C) BCRP efflux in AO enteroids (donor Hu18-9jej) calculated by PhA accumulation; transporter substrate: PhA, transporter inhibitor: KO143. Results plotted as mean ± SD. Data are shown as triplicates in three independent experiments; data points of each independent experiment denoted as an individual shape.

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: BCRP expression, localization and activity in AO enteroids in suspension (A) Schematic illustrating BCRP efflux under physiological function, inhibition or absence (e.g., knockout). (B) Representative confocal images following staining with DAPI (nuclei; blue), BCRP (green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the BCRP transporter in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (C) BCRP efflux in AO enteroids (donor Hu18-9jej) calculated by PhA accumulation; transporter substrate: PhA, transporter inhibitor: KO143. Results plotted as mean ± SD. Data are shown as triplicates in three independent experiments; data points of each independent experiment denoted as an individual shape.

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Expressing, Activity Assay, Suspension, Inhibition, Knock-Out, Confocal, Staining

    CYP3A4 metabolic activity in AO enteroids in suspension and PBPK simulation (A) Representative confocal images following staining with DAPI (nuclei; blue), CYP3A4 (green), and phalloidin (F-actin; magenta), highlighting the expression pattern of the CYP3A4 metabolic enzyme in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B) CYP3A4 metabolic activity in AO enteroids (donor Hu18-9jej) over a time period of 120 min. The concentration of the enzyme substrate terfenadine was monitored over time, as well as the formation of its metabolite fexofenadine. Data are shown as mean ± SD. (C) Intrinsic clearance of terfenadine in AO enteroids (donor: Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej). Data are shown as median ± IQR, ∗∗∗ p < 0.001 (Mann-Whitney U test). (D) Corrected intrinsic clearance of terfenadine considering the differences in CYP3A4 protein expression in AO enteroids (donor Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej. Data are shown as median ± IQR. Mann-Whitney U test showed no statistically significant difference between groups. (E) Simulated mean pharmacokinetics profile of a 60 mg dose of terfenadine in 140 virtual males (20–50 years, black line) with its 5th and 95th percentile profile shown as dashed lines. The green line represents the PBPK model mean prediction for AO enteroids (log concentration). The orange dots depict mean observed data from Okerholm et al. See also ; .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: CYP3A4 metabolic activity in AO enteroids in suspension and PBPK simulation (A) Representative confocal images following staining with DAPI (nuclei; blue), CYP3A4 (green), and phalloidin (F-actin; magenta), highlighting the expression pattern of the CYP3A4 metabolic enzyme in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B) CYP3A4 metabolic activity in AO enteroids (donor Hu18-9jej) over a time period of 120 min. The concentration of the enzyme substrate terfenadine was monitored over time, as well as the formation of its metabolite fexofenadine. Data are shown as mean ± SD. (C) Intrinsic clearance of terfenadine in AO enteroids (donor: Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej). Data are shown as median ± IQR, ∗∗∗ p < 0.001 (Mann-Whitney U test). (D) Corrected intrinsic clearance of terfenadine considering the differences in CYP3A4 protein expression in AO enteroids (donor Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej. Data are shown as median ± IQR. Mann-Whitney U test showed no statistically significant difference between groups. (E) Simulated mean pharmacokinetics profile of a 60 mg dose of terfenadine in 140 virtual males (20–50 years, black line) with its 5th and 95th percentile profile shown as dashed lines. The green line represents the PBPK model mean prediction for AO enteroids (log concentration). The orange dots depict mean observed data from Okerholm et al. See also ; .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Activity Assay, Suspension, Confocal, Staining, Expressing, Concentration Assay, MANN-WHITNEY, Drug discovery

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet:

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Tissue, Recombinant, Membrane, Gentle, Cell Recovery, Staining, Plasmid Preparation, Saline, Viability Assay, Lysis, Mass Spectrometry, Software