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pa5  (Proteintech)


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    Structured Review

    Proteintech pa5
    Pa5, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 4856 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/v5+custom+microarray+chip/Caspase+3%2Fp17%2Fp19+Antibody/pm37951219-765-94-119
    Average 97 stars, based on 4856 article reviews
    pa5 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Cell-Signaling:

    Article Title: Intracellular Staphylococcus aureus triggers isolate-specific host transcriptional responses alongside TNF-R1 regulated cell fate decisions
    Article Snippet: The peroxidase-conjugated secondary antibodies were incubated for 1 h, diluted 1:10,000 in TBS-T. Blots were imaged using ECL (RPN2232, Cytiva) and the ImageQuant800 device (Cytiva). .. Primary antibodies: PARP-1 (cs #9542S), cleaved caspase-3 (#9661S, Cell Signaling), caspase-3 (#9668S, Cell Signaling), Actin-HRP (HRP-60008, Proteintech). .. Secondary antibody: anti-rabbit-HRP (111–035–144, Jackson ImmunoResearch).

    Immunofluorescence:

    Article Title: Tumor Treating Fields Enhance Temozolomide‐Associated Growth Suppression in Glioblastoma Cells and Patient‐Derived Organoids Through Cell‐Cycle Blockade, Apoptosis, and SMAD ‐Associated Signaling
    Article Snippet: .. For cleaved caspase‐3 immunofluorescence, cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X‐100, blocked with 5% BSA, and incubated overnight at 4°C with anti‐cleaved caspase‐3 antibody (Proteintech, USA; Cat. No. 25546–1‐AP; 1:200). .. Cells were then incubated with Alexa Fluor 594‐conjugated secondary antibody (Invitrogen, USA; Cat. No. A‐11037; 1:500), counterstained with DAPI (1 μg/mL), and imaged using a BZ‐X810 fluorescence microscope (Keyence, Japan).

    Incubation:

    Article Title: Tumor Treating Fields Enhance Temozolomide‐Associated Growth Suppression in Glioblastoma Cells and Patient‐Derived Organoids Through Cell‐Cycle Blockade, Apoptosis, and SMAD ‐Associated Signaling
    Article Snippet: .. For cleaved caspase‐3 immunofluorescence, cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X‐100, blocked with 5% BSA, and incubated overnight at 4°C with anti‐cleaved caspase‐3 antibody (Proteintech, USA; Cat. No. 25546–1‐AP; 1:200). .. Cells were then incubated with Alexa Fluor 594‐conjugated secondary antibody (Invitrogen, USA; Cat. No. A‐11037; 1:500), counterstained with DAPI (1 μg/mL), and imaged using a BZ‐X810 fluorescence microscope (Keyence, Japan).

    Article Title: Integrated transcriptomic and deep learning analyses define acRG signature for prognosis and therapeutic options in osteosarcoma.
    Article Snippet: Osteosarcoma is a highly malignant bone tumor characterized by significant clinical heterogeneity and poor prognostic outcomes.. In this study, we integrated single-cell RNA sequencing data to characterize the landscape of genes related to N-acetylcytidine (ac4C) modification.. We calculated an acRG activity score and found that activity was predominantly enriched in osteoblastic cells.

    Blocking Assay:

    Article Title: Integrated transcriptomic and deep learning analyses define acRG signature for prognosis and therapeutic options in osteosarcoma.
    Article Snippet: Osteosarcoma is a highly malignant bone tumor characterized by significant clinical heterogeneity and poor prognostic outcomes.. In this study, we integrated single-cell RNA sequencing data to characterize the landscape of genes related to N-acetylcytidine (ac4C) modification.. We calculated an acRG activity score and found that activity was predominantly enriched in osteoblastic cells.

    Tissue:

    Article Title: Glucose-induced histone lactylation confers metformin resistance in colorectal cancer stem cells.
    Article Snippet: .. Tissue samples were fixed in formalin, embedded in paraffin, and subjected to immunohistochemical staining to evaluate cell proliferation and apoptosis using antibodies against Ki-67 (Proteintech, 28074-1-AP, 1:1000) and Cleaved Caspase-3 (Proteintech, 25128-1-AP, 1:5000), respectively. .. CD133+ CSC regions were identified by staining with an anti-CD133 antibody (Cell Signaling Technology, 64326, 1:200).

    Immunohistochemical staining:

    Article Title: Glucose-induced histone lactylation confers metformin resistance in colorectal cancer stem cells.
    Article Snippet: .. Tissue samples were fixed in formalin, embedded in paraffin, and subjected to immunohistochemical staining to evaluate cell proliferation and apoptosis using antibodies against Ki-67 (Proteintech, 28074-1-AP, 1:1000) and Cleaved Caspase-3 (Proteintech, 25128-1-AP, 1:5000), respectively. .. CD133+ CSC regions were identified by staining with an anti-CD133 antibody (Cell Signaling Technology, 64326, 1:200).

    Staining:

    Article Title: Glucose-induced histone lactylation confers metformin resistance in colorectal cancer stem cells.
    Article Snippet: .. Tissue samples were fixed in formalin, embedded in paraffin, and subjected to immunohistochemical staining to evaluate cell proliferation and apoptosis using antibodies against Ki-67 (Proteintech, 28074-1-AP, 1:1000) and Cleaved Caspase-3 (Proteintech, 25128-1-AP, 1:5000), respectively. .. CD133+ CSC regions were identified by staining with an anti-CD133 antibody (Cell Signaling Technology, 64326, 1:200).

    Hemagglutination Assay:

    Article Title: Obesity-Induced PLPE-PTK6-FASN Feedback Loop Drives Enzalutamide Resistance by Exacerbating Lipid Metabolic Dysregulation in CRPC
    Article Snippet: .. PTK6 (#A7497, RRID: -, ABclonal, 1:1000 dilution), p-PTK6 (phospho Y342) (#PA564567, Invitrogen, 1:1000 dilution), ATP1A1 (#14418-1-AP, RRID: AB_2227873, Proteintech, 1:10000 dilution), Cleaved caspase-3 (#25128-1-AP, RRID: AB_3073913, Proteintech, 1:800 dilution), FASN (#66591-1-Ig, RRID: AB_2881951, Proteintech, 1:10000 dilution), p-FASN (phospho T976) (# TP71286, HuaBio, 1:1000 dilution), PRKCZ (#26899-1-AP, RRID: AB_2880675, Proteintech, 1:2000 dilution), PPP5C (Cat# 11715-1-AP, RRID: AB_2237478, Proteintech, 1:1000 dilution), p-Tyr (phospho-Tyrosine, #14-5001-82, RRID: AB_2572884, Invitrogen, 1:500 dilution), pSer/Thr (Phospho-serine/threonine, # MA5-38234, RRID: AB_2898150, Invitrogen, 1:5000 dilution), β-actin (#20536-1-AP, RRID: AB_10700003, Proteintech, 1:3000 dilution), Flag-tag (#20543-1-AP, RRID: AB_11232216, Proteintech, 1:50000 dilution), HA-tag (#51064-2-AP, RRID: AB_11042321, Proteintech, 1:8000 dilution), Myc-tag (#60003-2-Ig, RRID: AB_2734122, Proteintech, 1:10000 dilution), GST-Tag (#10000-0-AP, Proteintech, 1:2000 dilution). .. Enzalutamide (MedChemExpress, Cat#HY-70002); PLPE (Macklin, Cat#P969978), Biotin-PLPE (Ruixibio, Cat# R-HFD10), Tilfrinib (MedChemExpress, Cat#HY110244), TVB2640 (MedChemExpress, Cat#HY-112829), TVB3664 (MedChemExpress, Cat#HY-120062), Streptavidin(SAV)MagBeads (YEASEN, Cat# 2026 47503ES03)

    Western Blot:

    Article Title: A novel tRNA-derived fragment, tRF-16-WSNKP9D promotes malignant progression of colorectal carcinoma through mediating PFN1.
    Article Snippet: .. Immunoblotting procedures incorporated a specific panel of primary antibodies, including anti-GAPDH (60004-1- Ig), anti-PFN1 (11680-1-AP), anti-BAX (50599-2-Ig), anti-BCL-2 (12789-1-AP), and anti-cleaved caspase 3 (68773-1-Ig), all sourced from Proteintech (Shanghai, China). .. Additionally, downstream molecular interactions were interrogated utilizing the Pierce Magnetic RNA-Protein Pull-Down system (Thermo Fisher Scientific).



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