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gblock gene fragments  (Integrated DNA Technologies)


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    Integrated DNA Technologies gblock gene fragments
    Gblock Gene Fragments, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 98/100, based on 4446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/user+defined+material+subroutine+(umat)+program/gBlocks+Gene+Fragments/pmc06506237-136-11-14
    Average 98 stars, based on 4446 article reviews
    gblock gene fragments - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Sequencing:

    Article Title: Germline T cell receptor exchange results in physiological T cell development and function
    Article Snippet: .. The Trac HA-TCR-GSG-T2A sequence was synthesized as a gBlock Gene Fragments (IDT, Coralville, IA) with AttB sites and subcloned into pDONR221 using the Gateway BP Clonase II Enzyme Mix (ThermoFisher Scientific, Waltham, MA) to produce pENTR-mTRAC HA. .. TCR sequences were codon optimized and synthesized by Genscript and subsequently cloned into pENTR-mTRAC HA using Gibson Assembly .

    Article Title: HAPSTR1 localizes HUWE1 to the nucleus to limit stress signaling pathways
    Article Snippet: .. The plasmid expressing pegRNA was cloned via Gibson assembly using a gBlock gene fragment containing the U6 promoter and the pegRNA sequence (purchased from IDT) and a linearized pBluescript vector. .. Hap1 cells were seeded in 6-well plates and transfected at roughly 70% confluency with lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions and 500 ng pegRNA plasmid and 1 μg PE plasmid expressing the prime editor (PE2-2A-GFP, Addgene #132776).

    Synthesized:

    Article Title: Germline T cell receptor exchange results in physiological T cell development and function
    Article Snippet: .. The Trac HA-TCR-GSG-T2A sequence was synthesized as a gBlock Gene Fragments (IDT, Coralville, IA) with AttB sites and subcloned into pDONR221 using the Gateway BP Clonase II Enzyme Mix (ThermoFisher Scientific, Waltham, MA) to produce pENTR-mTRAC HA. .. TCR sequences were codon optimized and synthesized by Genscript and subsequently cloned into pENTR-mTRAC HA using Gibson Assembly .

    Article Title: Tregs constrain CD8 + T cell priming required for curative intratumorally anchored anti-4-1BB immunotherapy
    Article Snippet: .. The heavy chain and light chain variable regions of α4-1BB antibody (clone LOB12.3) were synthesized as gBlock gene fragments (Integrated DNA technologies) and cloned into the gWiz expression vector (Genlantis) using In-fusion cloning (Takara Bio). ..

    Article Title: Virus-host dynamics in archaeal groundwater biofilms and the associated bacterial community composition
    Article Snippet: .. The polynucleotides were chemically synthesized by IDT (Integrated DNA Technologies, CA, USA) as gBlocks ® Gene Fragments. .. All eleven polynucleotides from each probe set were mixed in equimolar ratios and then labelled as previously described [ ], using the ULYSIS TM Alexa Fluor TM 594 nucleic acid labeling kit (Thermo Fisher Scientific, MA, USA).

    Article Title: Tregs constrain CD8 + T cell priming required for curative intratumorally anchored anti-4-1BB immunotherapy
    Article Snippet: .. For LAIR fusions, the murine LAIR1 gene was synthesized as a gBlock gene fragment (Integrated DNA technologies) and cloned as a fusion to the C-terminus of the heavy chain constant region separated by a flexible (G 4 S) 3 linker. .. Plasmids were transformed into Stellar competent cells (Takara Bio) for amplification and isolated with Nucleobond Xtra endotoxin-free kits (Macherey-Nagel). a4-1BB, a4-1BB-LAIR, αFITC, and αFITC-LAIR were produced using the Expi293 expression system (Gibco) following manufacturer’s instructions.

    Clone Assay:

    Article Title: Tregs constrain CD8 + T cell priming required for curative intratumorally anchored anti-4-1BB immunotherapy
    Article Snippet: .. The heavy chain and light chain variable regions of α4-1BB antibody (clone LOB12.3) were synthesized as gBlock gene fragments (Integrated DNA technologies) and cloned into the gWiz expression vector (Genlantis) using In-fusion cloning (Takara Bio). ..

    Article Title: Tregs constrain CD8 + T cell priming required for curative intratumorally anchored anti-4-1BB immunotherapy
    Article Snippet: .. For LAIR fusions, the murine LAIR1 gene was synthesized as a gBlock gene fragment (Integrated DNA technologies) and cloned as a fusion to the C-terminus of the heavy chain constant region separated by a flexible (G 4 S) 3 linker. .. Plasmids were transformed into Stellar competent cells (Takara Bio) for amplification and isolated with Nucleobond Xtra endotoxin-free kits (Macherey-Nagel). a4-1BB, a4-1BB-LAIR, αFITC, and αFITC-LAIR were produced using the Expi293 expression system (Gibco) following manufacturer’s instructions.

    Article Title: HAPSTR1 localizes HUWE1 to the nucleus to limit stress signaling pathways
    Article Snippet: .. The plasmid expressing pegRNA was cloned via Gibson assembly using a gBlock gene fragment containing the U6 promoter and the pegRNA sequence (purchased from IDT) and a linearized pBluescript vector. .. Hap1 cells were seeded in 6-well plates and transfected at roughly 70% confluency with lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions and 500 ng pegRNA plasmid and 1 μg PE plasmid expressing the prime editor (PE2-2A-GFP, Addgene #132776).

    Expressing:

    Article Title: Tregs constrain CD8 + T cell priming required for curative intratumorally anchored anti-4-1BB immunotherapy
    Article Snippet: .. The heavy chain and light chain variable regions of α4-1BB antibody (clone LOB12.3) were synthesized as gBlock gene fragments (Integrated DNA technologies) and cloned into the gWiz expression vector (Genlantis) using In-fusion cloning (Takara Bio). ..

    Article Title: HAPSTR1 localizes HUWE1 to the nucleus to limit stress signaling pathways
    Article Snippet: .. The plasmid expressing pegRNA was cloned via Gibson assembly using a gBlock gene fragment containing the U6 promoter and the pegRNA sequence (purchased from IDT) and a linearized pBluescript vector. .. Hap1 cells were seeded in 6-well plates and transfected at roughly 70% confluency with lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions and 500 ng pegRNA plasmid and 1 μg PE plasmid expressing the prime editor (PE2-2A-GFP, Addgene #132776).

    Plasmid Preparation:

    Article Title: Tregs constrain CD8 + T cell priming required for curative intratumorally anchored anti-4-1BB immunotherapy
    Article Snippet: .. The heavy chain and light chain variable regions of α4-1BB antibody (clone LOB12.3) were synthesized as gBlock gene fragments (Integrated DNA technologies) and cloned into the gWiz expression vector (Genlantis) using In-fusion cloning (Takara Bio). ..

    Article Title: HAPSTR1 localizes HUWE1 to the nucleus to limit stress signaling pathways
    Article Snippet: .. The plasmid expressing pegRNA was cloned via Gibson assembly using a gBlock gene fragment containing the U6 promoter and the pegRNA sequence (purchased from IDT) and a linearized pBluescript vector. .. Hap1 cells were seeded in 6-well plates and transfected at roughly 70% confluency with lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions and 500 ng pegRNA plasmid and 1 μg PE plasmid expressing the prime editor (PE2-2A-GFP, Addgene #132776).

    Cloning:

    Article Title: Tregs constrain CD8 + T cell priming required for curative intratumorally anchored anti-4-1BB immunotherapy
    Article Snippet: .. The heavy chain and light chain variable regions of α4-1BB antibody (clone LOB12.3) were synthesized as gBlock gene fragments (Integrated DNA technologies) and cloned into the gWiz expression vector (Genlantis) using In-fusion cloning (Takara Bio). ..

    Generated:

    Article Title: Highly socially vulnerable communities exhibit disproportionately increased viral loads as measured in community wastewater
    Article Snippet: .. The standard curve was generated from a gBlock Gene Fragment (Integrated DNA Technologies; Coralville, IA) PMMoV Standard (1.0 × 10 8 PMMoV GC per 5 μL) ten-fold serial dilutions (1.0 × 10 8 –1.0 × 10 2 PMMoV GC per 5 μL). .. All figures were illustrated with the GraphPad SoftwareTM in the GraphPad Prism 9.3.1 program (Dotmatics; Boston, MA).

    Amplification:

    Article Title: Meiotic nuclear pore complex remodeling provides key insights into nuclear basket organization
    Article Snippet: .. This plasmid (pUB1729) and the appropriate repair template, amplified from a gBlock gene fragment (IDT), were cotransformed into yeast. .. This plasmid (pUB1729) and the appropriate repair template, amplified from a gBlock gene fragment (IDT), were cotransformed into yeast.



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