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upright fluorescence microscope  (Nikon)


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    Structured Review

    Nikon upright fluorescence microscope
    Upright Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/upright+fluorescence+microscope/C2%2B/pmc12966746-159-5-8
    Average 99 stars, based on 1 article reviews
    upright fluorescence microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Microscopy:


    Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification
    Article Snippet: The samples were then incubated with a Cy3-conjugated secondary antibody (Servicebio, China) for 50 min. .. Images were captured using an upright fluorescence microscope (NIKON Eclipse C1, Japan). .. Quantitative analysis of the positive immunohistochemistry signals was performed using ImageJ software.

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a confocal microscope (Nikon Eclipse C2), and the red/green intensity ratio was used to assess mitochondrial membrane potential (ΔΨm). ..

    Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
    Article Snippet: .. Confocal imaging was performed using a laser scanning confocal microscope (NIKON Eclipse Ti) equipped with the Eclipse C2 system (Nikon). .. Representative images were acquired and processed using Nikon imaging software.

    Article Title: Norepinephrine neurons in the locus coeruleus and the nucleus of the solitary tract drive different stress-related behavioral outputs in mice
    Article Snippet: .. They were then frozen and sectioned at 30 μm on a freezing microtome (HM550, Microm), mounted on microscope slides with Fluoroshield with DAPI mounting medium (F6057, Sigma-Aldrich), coverslipped, and imaged on a confocal microscope (Nikon C2). ..

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three PBS washes, fluorescent secondary antibodies (Servicebio, Alexa Fluor 488, GB25303; Alexa Fluor 594, GB28303) were incubated for 1 h in the dark, nuclei were counterstained with DAPI (1 μg mL −1 , 5 min, Servicebio, G1012), and images were acquired on a Nikon Eclipse C2 confocal microscope (Japan). ..

    Article Title: circOMA1 delivered by exosomes regulates DRD2-mediated prolactinoma resistance
    Article Snippet: The circOMA1 hybridisation probe was designed and synthesised by Shanghai GenePharma, Co., Ltd. RNA-FISH was performed on circOM cells and tissues using an in-situ hybridisation kit (Exon Biotech Inc.), according to the manufacturer's protocol. .. Images were obtained on a Nikon C2 laser-scanning confocal microscope. ..

    Fluorescence:

    Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification
    Article Snippet: The samples were then incubated with a Cy3-conjugated secondary antibody (Servicebio, China) for 50 min. .. Images were captured using an upright fluorescence microscope (NIKON Eclipse C1, Japan). .. Quantitative analysis of the positive immunohistochemistry signals was performed using ImageJ software.

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a confocal microscope (Nikon Eclipse C2), and the red/green intensity ratio was used to assess mitochondrial membrane potential (ΔΨm). ..

    Confocal:

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a confocal microscope (Nikon Eclipse C2), and the red/green intensity ratio was used to assess mitochondrial membrane potential (ΔΨm). ..

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three PBS washes, fluorescent secondary antibodies (Servicebio, Alexa Fluor 488, GB25303; Alexa Fluor 594, GB28303) were incubated for 1 h in the dark, nuclei were counterstained with DAPI (1 μg mL −1 , 5 min, Servicebio, G1012), and images were acquired on a Nikon Eclipse C2 confocal microscope (Japan). ..

    Membrane:

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a confocal microscope (Nikon Eclipse C2), and the red/green intensity ratio was used to assess mitochondrial membrane potential (ΔΨm). ..

    Incubation:

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7
    Article Snippet: To visualize biofilm formation and exopolysaccharide production, cultures were stained with Calcofluor to a final concentration of 85 μM, and 1 mM of propidium iodide (PI) (Sigma Aldrich, United States). .. After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Eclipse Ti-E C2+ confocal laser scanning microscope (Nikon) equipped with CFI Plan Apo VC 20x/1.2 and CFI Plan Apo VC 60x/1.2 WI objective lenses. ..

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three PBS washes, fluorescent secondary antibodies (Servicebio, Alexa Fluor 488, GB25303; Alexa Fluor 594, GB28303) were incubated for 1 h in the dark, nuclei were counterstained with DAPI (1 μg mL −1 , 5 min, Servicebio, G1012), and images were acquired on a Nikon Eclipse C2 confocal microscope (Japan). ..

    Laser-Scanning Microscopy:

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7
    Article Snippet: To visualize biofilm formation and exopolysaccharide production, cultures were stained with Calcofluor to a final concentration of 85 μM, and 1 mM of propidium iodide (PI) (Sigma Aldrich, United States). .. After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Eclipse Ti-E C2+ confocal laser scanning microscope (Nikon) equipped with CFI Plan Apo VC 20x/1.2 and CFI Plan Apo VC 60x/1.2 WI objective lenses. ..

    Imaging:

    Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
    Article Snippet: .. Confocal imaging was performed using a laser scanning confocal microscope (NIKON Eclipse Ti) equipped with the Eclipse C2 system (Nikon). .. Representative images were acquired and processed using Nikon imaging software.



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    OGD induces ferroptosis in HUVECs. (A) CCK-8 assay showing that OGD decreases HUVEC viability. Cell viability was restored in the si-lnc group. (B) Lnc_011797 si-lnc effectively inhibited lnc-011797 expression. The data were normalized to the control group. (C) The intracellular iron concentration in the OGD group was significantly higher than that in the control group and the OGD + si-lnc group. (D, E) A microplate reader (D) and <t>fluorescence</t> <t>microscope</t> (E) were used to show that the relative ROS concentration (green fluorescence) increased after OGD and decreased after lnc_011797 knockdown. The data were normalized to the control group. Scale bars: 50 μm. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance followed by the least significant difference post hoc tests). (F) Electron microscopy images of mitochondria. The mitochondrial morphology was normal in the control group. Mitochondrial cristae were decreased, membrane density was increased, and average mitochondrial length was decreased in the OGD and nc-lnc groups compared with the control group. The mitochondrial damage was alleviated in the si-lnc group. Scale bars: 1 μm. CCK-8: Cell counting Kit-8; HUVECs: human umbilical vein endothelial cell; OGD: oxygen-glucose deprivation; ROS: reactive oxygen species.
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    Image Search Results


    OGD induces ferroptosis in HUVECs. (A) CCK-8 assay showing that OGD decreases HUVEC viability. Cell viability was restored in the si-lnc group. (B) Lnc_011797 si-lnc effectively inhibited lnc-011797 expression. The data were normalized to the control group. (C) The intracellular iron concentration in the OGD group was significantly higher than that in the control group and the OGD + si-lnc group. (D, E) A microplate reader (D) and fluorescence microscope (E) were used to show that the relative ROS concentration (green fluorescence) increased after OGD and decreased after lnc_011797 knockdown. The data were normalized to the control group. Scale bars: 50 μm. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance followed by the least significant difference post hoc tests). (F) Electron microscopy images of mitochondria. The mitochondrial morphology was normal in the control group. Mitochondrial cristae were decreased, membrane density was increased, and average mitochondrial length was decreased in the OGD and nc-lnc groups compared with the control group. The mitochondrial damage was alleviated in the si-lnc group. Scale bars: 1 μm. CCK-8: Cell counting Kit-8; HUVECs: human umbilical vein endothelial cell; OGD: oxygen-glucose deprivation; ROS: reactive oxygen species.

    Journal: Neural Regeneration Research

    Article Title: Lnc_011797 promotes ferroptosis and aggravates white matter lesions

    doi: 10.4103/NRR.NRR-D-24-00676

    Figure Lengend Snippet: OGD induces ferroptosis in HUVECs. (A) CCK-8 assay showing that OGD decreases HUVEC viability. Cell viability was restored in the si-lnc group. (B) Lnc_011797 si-lnc effectively inhibited lnc-011797 expression. The data were normalized to the control group. (C) The intracellular iron concentration in the OGD group was significantly higher than that in the control group and the OGD + si-lnc group. (D, E) A microplate reader (D) and fluorescence microscope (E) were used to show that the relative ROS concentration (green fluorescence) increased after OGD and decreased after lnc_011797 knockdown. The data were normalized to the control group. Scale bars: 50 μm. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance followed by the least significant difference post hoc tests). (F) Electron microscopy images of mitochondria. The mitochondrial morphology was normal in the control group. Mitochondrial cristae were decreased, membrane density was increased, and average mitochondrial length was decreased in the OGD and nc-lnc groups compared with the control group. The mitochondrial damage was alleviated in the si-lnc group. Scale bars: 1 μm. CCK-8: Cell counting Kit-8; HUVECs: human umbilical vein endothelial cell; OGD: oxygen-glucose deprivation; ROS: reactive oxygen species.

    Article Snippet: Bright field images were taken at 4× and 60× magnification using an Olympus upright fluorescence microscope (BX53) (Poh et al., 2021).

    Techniques: CCK-8 Assay, Expressing, Control, Concentration Assay, Fluorescence, Microscopy, Knockdown, Electron Microscopy, Membrane, Cell Counting