ulk1  (Cell Signaling Technology Inc)

Journal: Diabetes, Metabolic Syndrome and Obesity

Article Title: Yiqi Huoxue Yangyin Decoction Ameliorates Diabetic Nephropathy by Targeting AMPK/mTOR-Mediated Autophagy: A Network Pharmacology-Guided in vivo and in vitro Study

doi: 10.2147/DMSO.S584822

Figure Lengend Snippet: Effects of YHY on the expressions of P62, ULK1, and LC3B in the kidneys of db/db mice. ( A ) Representative images of ULK1, P62, and LC3B expression (200×; scale bar = 50 μm). ( B and C ) Relative expression of ULK1 and P62 by IHC. ( D ) Relative expression of LC3B by IF. Data were shown as mean ± SD, n = 5, *** P < 0.001 vs normal group; ## P < 0.01, ### P < 0.001 vs model group.

Article Snippet: For immunohistochemistry (IHC), sections were incubated overnight at 4°C with primary antibodies against P62 (0.1 μg/mL, AB109012 , Abcam), ULK1 (1:100, A8529, ABclonal), and p-mTOR (0.1 μg/mL, AB109268 , Abcam), then incubated with HRP-conjugated secondary antibody and visualized with DAB.

Techniques: Expressing

Journal: Diabetes, Metabolic Syndrome and Obesity

Article Title: Yiqi Huoxue Yangyin Decoction Ameliorates Diabetic Nephropathy by Targeting AMPK/mTOR-Mediated Autophagy: A Network Pharmacology-Guided in vivo and in vitro Study

doi: 10.2147/DMSO.S584822

Figure Lengend Snippet: Effects of YHY on renal protein expressions in diabetic mice. ( A and B ) Representative images and relative expression of p-mTOR by IHC. ( C and D ) Western blot analysis of AMPK, p-AMPK, mTOR, p-mTOR, Nephrin, ULK1, Beclin-1, and P62 protein expression. Data were shown as mean ± SD, * P < 0.05 and ** P < 0.01, *** P < 0.001 vs normal group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs model group.

Article Snippet: For immunohistochemistry (IHC), sections were incubated overnight at 4°C with primary antibodies against P62 (0.1 μg/mL, AB109012 , Abcam), ULK1 (1:100, A8529, ABclonal), and p-mTOR (0.1 μg/mL, AB109268 , Abcam), then incubated with HRP-conjugated secondary antibody and visualized with DAB.

Techniques: Expressing, Western Blot

Journal: Diabetes, Metabolic Syndrome and Obesity

Article Title: Yiqi Huoxue Yangyin Decoction Ameliorates Diabetic Nephropathy by Targeting AMPK/mTOR-Mediated Autophagy: A Network Pharmacology-Guided in vivo and in vitro Study

doi: 10.2147/DMSO.S584822

Figure Lengend Snippet: Effects of YHY on high-glucose-induced autophagy in MPC-5 cells. ( A ) The mRNA expressions of autophagy (ULK1, Beclin-1, LC3B, ATG5, ATG7). ( B and C ) Western blot analysis of AMPK, p-AMPK, mTOR, p-mTOR, ULK1, Beclin-1, P62 and LC3 protein expression. Data were shown as mean ± SD, n = 3, * P < 0.05 and ** P < 0.01, *** P < 0.001 vs NG; # P < 0.05, ## P < 0.01, ### P < 0.001 vs HG.

Article Snippet: For immunohistochemistry (IHC), sections were incubated overnight at 4°C with primary antibodies against P62 (0.1 μg/mL, AB109012 , Abcam), ULK1 (1:100, A8529, ABclonal), and p-mTOR (0.1 μg/mL, AB109268 , Abcam), then incubated with HRP-conjugated secondary antibody and visualized with DAB.

Techniques: Western Blot, Expressing