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high-density oligonucleotide array human hg-u133a microarrays (platforms gpl3921 and gpl4685) (Thermo Fisher)

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Thermo Fisher high-density oligonucleotide array human hg-u133a microarrays (platforms gpl3921 and gpl4685)
High Density Oligonucleotide Array Human Hg U133a Microarrays (Platforms Gpl3921 And Gpl4685), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u133a+oligonucleotide+microarray+platform/pmc05077963-232-9-8
Average 90 stars, based on 1 article reviews

high-density oligonucleotide array human hg-u133a microarrays (platforms gpl3921 and gpl4685) - by Bioz Stars, 2026-11

90/100 stars

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Microarray:

Article Title: Single-Cell Heterogeneity of EGFR Pathway Is Linked to Unique Signatures of Drug Response and Malignancy in Patient Derived Glioblastoma Stem Cells.
Article Snippet: .. Because of the lack of gene name overlap from the Affymetrix U133A array used by TCGA and the Affymetrix U133 plus 2.0 microarray used for our classifications, only 789 of the original 840 genes were used to classify the samples. ..

Article Title: Single-Cell Heterogeneity of EGFR Pathway Is Linked to Unique Signatures of Drug Response and Malignancy in Patient Derived Glioblastoma Stem Cells.
Article Snippet: .. All rights reserved. gene name overlap from the Affymetrix U133A array used by TCGA and the Affymetrix U133 plus 2.0 microarray used for our classifications, only 789 of the original 840 genes were used to classify the samples. ..

other:

Article Title: Influence of Mesalazine on Ferroptosis-Related Gene Expression in In Vitro Colorectal Cancer Culture.
Article Snippet: The analysis was conducted with GeneChipTM Human Genome U133A 2.0 Array in combination with the GeneChipTM 3′ IVT PLUS Reagent Kit (Applied Biosystems, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions.

Article Title: The Prognostic Significance of TRs in Hepatocellular Carcinoma: Insights from TCGA and GEO Databases
Article Snippet: GSE14520 , Roessler S et al. 2009 , 247 , 241 , Affymetrix Human Genome U133A 2.0 Array/Affymetrix HT Human Genome U133A Array.

Article Title: The Prognostic Significance of TRs in Hepatocellular Carcinoma: Insights from TCGA and GEO Databases
Article Snippet: GSE60502 , Kao KJ, 2014 , 18 , 18 , Affymetrix Human Genome U133A Array.

Article Title: Alternative Complement Pathway in Carotid Atherosclerosis: Low Plasma Properdin Levels Associate With Long‐Term Cardiovascular Mortality
Article Snippet: The Human Genome U133A 2.0 Array encoding 14 500 genes was purchased from Affymetrix (Santa Clara, CA), and hybridization was performed according to the manufacturer’s 2- cycle target labeling protocol as previously described.22

Generated:

Article Title: Silencing of O-GlcNAc transferase attenuated O-GlcNAcylation and metastatic potentials of melanoma cells through suppression of Akt-NFkB signaling pathway.
Article Snippet: O-GlcNAcylation is an important biological process in regulating the function of many nucleocytoplasmic proteins in cells.. Enhancement of O-GlcNAcylation was associated with cancer development and progression.. Here, we demonstrated the involvement of O-GlcNAcylation in melanoma metastasis.

Gene Expression:

Article Title: Mature B-Cell Lymphoma And Leukemia Study III
Article Snippet: .. Measure Description: Gene expression levels in BL vs. non-BL will be analyzed through approximately 22,000 probesets on the Affymetrix U133A GeneChip by using two-factor analysis of variance model for each gene.. Time Frame: 1 year after the last participant is enrolled ..

Image Search Results

The fidelity and reproducibility of microarray profiling. (A) The CapitalBio's long oligonucleotide (Operon 70-mer) microarray and Affymetrix's short oligonucleotide (25-mer) HG U133A 2.0 microarray platform were compared, and the correlation coefficient (R value) between the two platforms was 0.787 when the total of 6754 common genes were detected using the two platforms, employing the same batch of RNA extracted from HeLa and HEK293 cell lines. (B) Self-to-self comparison of the gene expression. For each test and control sample, two hybridizations were performed by using a reversal fluorescence strategy. The change of magnitude of self-to-self expression profiling was within twofold, which indicated that a change less than twofold could be considered to represent the noise level of the microarray experiment. The two lines parallel in the graph represent 2- and 0.5-fold changes in expression. (C) The determination of global gene expression of HepG2.2.15 versus HepG2 cells was repeated three times independently. For each test and control sample in one independent experiment, two hybridizations were performed by using a reverse fluorescence strategy. Two sets of the expression ratios were chosen to determine the reproducibility of microarray results. The Pearson linear correlation coefficient value (R) was 0.956. (D) Comparison of expression measurement by quantitative RT-PCR and microarray. The expression changes in the selected 17 genes showed good agreement between the two methods.

Journal:

Article Title: Genomic Analysis of Anti-Hepatitis B Virus (HBV) Activity by Small Interfering RNA and Lamivudine in Stable HBV-Producing Cells

doi: 10.1128/JVI.79.22.14392-14403.2005

Figure Lengend Snippet: The fidelity and reproducibility of microarray profiling. (A) The CapitalBio's long oligonucleotide (Operon 70-mer) microarray and Affymetrix's short oligonucleotide (25-mer) HG U133A 2.0 microarray platform were compared, and the correlation coefficient (R value) between the two platforms was 0.787 when the total of 6754 common genes were detected using the two platforms, employing the same batch of RNA extracted from HeLa and HEK293 cell lines. (B) Self-to-self comparison of the gene expression. For each test and control sample, two hybridizations were performed by using a reversal fluorescence strategy. The change of magnitude of self-to-self expression profiling was within twofold, which indicated that a change less than twofold could be considered to represent the noise level of the microarray experiment. The two lines parallel in the graph represent 2- and 0.5-fold changes in expression. (C) The determination of global gene expression of HepG2.2.15 versus HepG2 cells was repeated three times independently. For each test and control sample in one independent experiment, two hybridizations were performed by using a reverse fluorescence strategy. Two sets of the expression ratios were chosen to determine the reproducibility of microarray results. The Pearson linear correlation coefficient value (R) was 0.956. (D) Comparison of expression measurement by quantitative RT-PCR and microarray. The expression changes in the selected 17 genes showed good agreement between the two methods.

Article Snippet: The reason for this is that the fidelity of our microarray is twofold; therefore, genes with a change of less than twofold fell into the “gray zone” of microarray analysis, which means that the calculated up- or down-regulation change of these genes may not be precise, and hence it is reasonable to conclude that parts of these five genes may have some disparities in terms of their regulation changes when the two methodologies were applied. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 7. caption a7 The fidelity and reproducibility of microarray profiling. (A) The CapitalBio's long oligonucleotide (Operon 70-mer) microarray and Affymetrix's short oligonucleotide (25-mer) HG U133A 2.0 microarray platform were compared, and the correlation coefficient (R value) between the two platforms was 0.787 when the total of 6754 common genes were detected using the two platforms, employing the same batch of RNA extracted from HeLa and HEK293 cell lines. (B) Self-to-self comparison of the gene expression.

Techniques: Microarray, Expressing, Fluorescence, Quantitative RT-PCR