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rneasy  (Qiagen)


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  • 99

    Structured Review

    Qiagen rneasy
    Rneasy, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 18014 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u133a+microarray+data/RNeasy+Plus+Universal+Mini+Kit/pm17882285-28-29-31
    Average 99 stars, based on 18014 article reviews
    rneasy - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Bulk RNA Sequencing:

    Article Title: ACKR3 Establishes a Squamous-Restricted Immune Checkpoint Axis in Lung Squamous Cell Carcinoma
    Article Snippet: .. 271 272 273 Bulk RNA Sequencing and Data Analysis 274 275 Total RNA was extracted from PL and PLA murine cell lines using the RNeasy Plus 276 Universal Mini Kit (QIAGEN) according to the manufacturer’s instructions. .. cDNA 277 synthesis was performed using the SMARTer PCR cDNA Synthesis Kit (Clontech), 278 followed by library preparation with the Nextera XT DNA Library Preparation Kit (Illumina), 279 following standard protocols.

    Analysis:

    Article Title: ACKR3 Establishes a Squamous-Restricted Immune Checkpoint Axis in Lung Squamous Cell Carcinoma
    Article Snippet: .. 271 272 273 Bulk RNA Sequencing and Data Analysis 274 275 Total RNA was extracted from PL and PLA murine cell lines using the RNeasy Plus 276 Universal Mini Kit (QIAGEN) according to the manufacturer’s instructions. .. cDNA 277 synthesis was performed using the SMARTer PCR cDNA Synthesis Kit (Clontech), 278 followed by library preparation with the Nextera XT DNA Library Preparation Kit (Illumina), 279 following standard protocols.

    Article Title: IL-12-producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy.
    Article Snippet: Please cite this article as: Yamamoto M, Zhang R, Kobayashi T, Li Y, Xu H, Mashima H, Saito K, Kariya M, Ide M, Hagiya Y, Fukuda E, Uemura S, Osako Y, Nakaki R, Nakatsura T, Fukushima S, Kaneko S, Hotta A, Ohdan H, Uemura.. Y, IL-12–producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy, Molecular Therapy (2026), doi: https:// doi.org/10.1016/j.ymthe.2026.10.017.

    Proximity Ligation Assay:

    Article Title: ACKR3 Establishes a Squamous-Restricted Immune Checkpoint Axis in Lung Squamous Cell Carcinoma
    Article Snippet: .. 271 272 273 Bulk RNA Sequencing and Data Analysis 274 275 Total RNA was extracted from PL and PLA murine cell lines using the RNeasy Plus 276 Universal Mini Kit (QIAGEN) according to the manufacturer’s instructions. .. cDNA 277 synthesis was performed using the SMARTer PCR cDNA Synthesis Kit (Clontech), 278 followed by library preparation with the Nextera XT DNA Library Preparation Kit (Illumina), 279 following standard protocols.

    Isolation:

    Article Title: Microglia-mediated extracellular matrix disruption drives neuronal dysfunction, motor impairments, and sedation following alcohol abuse.
    Article Snippet: .. Isolation of liver RNA and qRT-PCR Total RNA was isolated from liver tissue using Rneasy Plus Universal Mini Kit (Qiagen) and reverse transcribed with SuperScript IV VILO Master Mix (Invitrogen), followed by amplification using qRT-PCR. .. Power SYBR Green PCR Master Mix (Applied Biosystems) and primers (listed below) at final concentrations of 1 μM were used.

    Quantitative RT-PCR:

    Article Title: Microglia-mediated extracellular matrix disruption drives neuronal dysfunction, motor impairments, and sedation following alcohol abuse.
    Article Snippet: .. Isolation of liver RNA and qRT-PCR Total RNA was isolated from liver tissue using Rneasy Plus Universal Mini Kit (Qiagen) and reverse transcribed with SuperScript IV VILO Master Mix (Invitrogen), followed by amplification using qRT-PCR. .. Power SYBR Green PCR Master Mix (Applied Biosystems) and primers (listed below) at final concentrations of 1 μM were used.

    Tissue:

    Article Title: Microglia-mediated extracellular matrix disruption drives neuronal dysfunction, motor impairments, and sedation following alcohol abuse.
    Article Snippet: .. Isolation of liver RNA and qRT-PCR Total RNA was isolated from liver tissue using Rneasy Plus Universal Mini Kit (Qiagen) and reverse transcribed with SuperScript IV VILO Master Mix (Invitrogen), followed by amplification using qRT-PCR. .. Power SYBR Green PCR Master Mix (Applied Biosystems) and primers (listed below) at final concentrations of 1 μM were used.

    Article Title: Development and Characterization of a Rat Model of Post-traumatic Ankle Osteoarthritis.
    Article Snippet: .. To get sufficient tissue for RNA sequencing, DRGs from 2-3 rats were pooled, and total RNA was extracted using an RNeasy Plus Universal Kit (Qiagen, Germantown, MD) according to the manufacturer's instructions. ..

    Reverse Transcription:

    Article Title: Microglia-mediated extracellular matrix disruption drives neuronal dysfunction, motor impairments, and sedation following alcohol abuse.
    Article Snippet: .. Isolation of liver RNA and qRT-PCR Total RNA was isolated from liver tissue using Rneasy Plus Universal Mini Kit (Qiagen) and reverse transcribed with SuperScript IV VILO Master Mix (Invitrogen), followed by amplification using qRT-PCR. .. Power SYBR Green PCR Master Mix (Applied Biosystems) and primers (listed below) at final concentrations of 1 μM were used.

    Amplification:

    Article Title: Microglia-mediated extracellular matrix disruption drives neuronal dysfunction, motor impairments, and sedation following alcohol abuse.
    Article Snippet: .. Isolation of liver RNA and qRT-PCR Total RNA was isolated from liver tissue using Rneasy Plus Universal Mini Kit (Qiagen) and reverse transcribed with SuperScript IV VILO Master Mix (Invitrogen), followed by amplification using qRT-PCR. .. Power SYBR Green PCR Master Mix (Applied Biosystems) and primers (listed below) at final concentrations of 1 μM were used.

    Cell Culture:

    Article Title: A natural compound derivative targeting HADHA disrupts mitochondrial homeostasis and suppresses renal cell carcinoma.
    Article Snippet: Ara Jo, Sanjeevram Dhandapani, Ziqin Wang, Hyeryeon Jung, Hongjun Jang, Morgan A. Bailey, Andrew Tran, Brandon Tsai, Jinjae Park, Hyejin Lee, Hye-Jin Park, Yoojin Lim, Dolce Feenaghty, Victor M. Moncada, Damu Wu, Keaton Chien, Nancy Chen, Yunsol Jo, Jiazheng Miao, Zhujun Yao, Jihoon Lee, Karthigayan Shanmugasundaram, Gang Greg Wang, Andrew Armstrong, Daniel George, David Howell, Jiaoti Huang, Michael C. Fitzgerald, Jiyong Hong, Jung Wook Park

    Crocin Bleaching Assay:

    Article Title: A natural compound derivative targeting HADHA disrupts mitochondrial homeostasis and suppresses renal cell carcinoma.
    Article Snippet: Ara Jo, Sanjeevram Dhandapani, Ziqin Wang, Hyeryeon Jung, Hongjun Jang, Morgan A. Bailey, Andrew Tran, Brandon Tsai, Jinjae Park, Hyejin Lee, Hye-Jin Park, Yoojin Lim, Dolce Feenaghty, Victor M. Moncada, Damu Wu, Keaton Chien, Nancy Chen, Yunsol Jo, Jiazheng Miao, Zhujun Yao, Jihoon Lee, Karthigayan Shanmugasundaram, Gang Greg Wang, Andrew Armstrong, Daniel George, David Howell, Jiaoti Huang, Michael C. Fitzgerald, Jiyong Hong, Jung Wook Park

    Lysis:

    Article Title: A natural compound derivative targeting HADHA disrupts mitochondrial homeostasis and suppresses renal cell carcinoma.
    Article Snippet: Ara Jo, Sanjeevram Dhandapani, Ziqin Wang, Hyeryeon Jung, Hongjun Jang, Morgan A. Bailey, Andrew Tran, Brandon Tsai, Jinjae Park, Hyejin Lee, Hye-Jin Park, Yoojin Lim, Dolce Feenaghty, Victor M. Moncada, Damu Wu, Keaton Chien, Nancy Chen, Yunsol Jo, Jiazheng Miao, Zhujun Yao, Jihoon Lee, Karthigayan Shanmugasundaram, Gang Greg Wang, Andrew Armstrong, Daniel George, David Howell, Jiaoti Huang, Michael C. Fitzgerald, Jiyong Hong, Jung Wook Park

    RNA Sequencing:

    Article Title: IL-12-producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy.
    Article Snippet: Please cite this article as: Yamamoto M, Zhang R, Kobayashi T, Li Y, Xu H, Mashima H, Saito K, Kariya M, Ide M, Hagiya Y, Fukuda E, Uemura S, Osako Y, Nakaki R, Nakatsura T, Fukushima S, Kaneko S, Hotta A, Ohdan H, Uemura.. Y, IL-12–producing iPSC-derived antigen-presenting cells enhance antitumor immunity and enable off-the-shelf immunotherapy, Molecular Therapy (2026), doi: https:// doi.org/10.1016/j.ymthe.2026.10.017.

    Article Title: Development and Characterization of a Rat Model of Post-traumatic Ankle Osteoarthritis.
    Article Snippet: .. To get sufficient tissue for RNA sequencing, DRGs from 2-3 rats were pooled, and total RNA was extracted using an RNeasy Plus Universal Kit (Qiagen, Germantown, MD) according to the manufacturer's instructions. ..

    Synthesized:

    Article Title: Mesenchymal stromal cells immunosuppress osteoarthritis synovial fluid modulated monocytes via IL-6 and CCL2.
    Article Snippet: .. Total RNA was extracted with RNeasy kit (Qiagen, 73404); cDNA was synthesized using SuperScript IV VILO master mix (ThermoFisher Scientific) per the manufacturer’s instructions. ..



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    Image Search Results


    Expression of the metagene clusters in immunological cell types. (a) The 199 ProbeSets from Figure 1a were used to cluster 44 samples of isolated cells and tissues with immune-system-related functions that were profiled on Affymetrix U133A arrays by Su and colleagues [GEO:GSE1133] . In each case, two samples for the following cell/tissue types are presented from left to right: fetal liver (1,2), K-562 (3,4), whole blood (5,6), CD33 myeloid (7,8), CD14 monocytes (9,10), CD34 (11,12), B lymphoblasts (13,14), CD56 natural killer cells (15,16), CD4 T cells (17,18), CD8 T cells (19,20), MOLT-4 (21,22), Raji (23,24), HL-60 (25,26), Daudi (27,28), CD105 (29,30), CD71 (31,32), BDCA4 dendritic cells (33,34), CD19 B cells (35,36), thymus (37,38), tonsil (39,40), lymph node (41,42), bone marrow (43,44). Details about the respective samples are given in Additional data file . HCK, hemopoietic cell kinase; LCK, lymphocyte-specific kinase; MHC, major histocompatibility complex; STAT1, signal transducer and activator of transcription 1. (b) Representation of the seven metagenes that were derived from the 199 ProbeSets as in Figure 1b.

    Journal: Breast Cancer Research : BCR

    Article Title: T-cell metagene predicts a favorable prognosis in estrogen receptor-negative and HER2-positive breast cancers

    doi: 10.1186/bcr2234

    Figure Lengend Snippet: Expression of the metagene clusters in immunological cell types. (a) The 199 ProbeSets from Figure 1a were used to cluster 44 samples of isolated cells and tissues with immune-system-related functions that were profiled on Affymetrix U133A arrays by Su and colleagues [GEO:GSE1133] . In each case, two samples for the following cell/tissue types are presented from left to right: fetal liver (1,2), K-562 (3,4), whole blood (5,6), CD33 myeloid (7,8), CD14 monocytes (9,10), CD34 (11,12), B lymphoblasts (13,14), CD56 natural killer cells (15,16), CD4 T cells (17,18), CD8 T cells (19,20), MOLT-4 (21,22), Raji (23,24), HL-60 (25,26), Daudi (27,28), CD105 (29,30), CD71 (31,32), BDCA4 dendritic cells (33,34), CD19 B cells (35,36), thymus (37,38), tonsil (39,40), lymph node (41,42), bone marrow (43,44). Details about the respective samples are given in Additional data file . HCK, hemopoietic cell kinase; LCK, lymphocyte-specific kinase; MHC, major histocompatibility complex; STAT1, signal transducer and activator of transcription 1. (b) Representation of the seven metagenes that were derived from the 199 ProbeSets as in Figure 1b.

    Article Snippet: Affymetrix HG-U133A microarray data of 1,781 primary breast cancer samples from 12 datasets were included.

    Techniques: Expressing, Isolation, Derivative Assay