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rneasy midi kit  (Qiagen)


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    Structured Review

    Qiagen rneasy midi kit
    Rneasy Midi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 5313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u133a+microarray+data/RNeasy+Midi+Kit/pm17713554-23-20-23
    Average 98 stars, based on 5313 article reviews
    rneasy midi kit - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Modification:

    Article Title: RNA binding proteome profiling reveals RNA-dependent activation of oncogenic host signaling during KSHV infection
    Article Snippet: .. QIAGEN RNeasy Midi Kit was used to purify peptide-crosslinked RNA with modified protocol. ..

    Purification:

    Article Title: Design and validation of a ribosome display library for synthetic nanobody selection
    Article Snippet: DNA (10 μg) was transcripted using the RiboMAXTM Large Scale RNA Production Systems (Cat. P1300, Promega). .. The product was purified using RNeasy Mid Kit (Cat. 75144, QIAGEN) to yield 360 μg mRNA in a total volume of 200 μL. ..

    RNA Extraction:

    Article Title: Temporal and spatial characterization of nicotinic acetylcholine receptor expression in Lepeophtheirus salmonis.
    Article Snippet: tinic acetylcholine receptors (nAChRs) are key mediators of synaptic transmission in invertebrates and m ts of pesticide action.. In early life stages of marine Copepoda, such as the parasitic salmon louse (Lepeo us salmonis), the expression dynamics and tissue localization of nAChRs remain poorly characterized.. H vestigate the expression of nAChR‐related transcripts across the life cycle of L. salmonis, and spatial di n of expression in the early copepodid life stage.

    Article Title: Impaired erythroid maturation in murine embryos upon loss of the preeclampsia-associated serine protease prostasin Prostasin loss impairs erythropoiesis
    Article Snippet: .. For RNA extraction, the tissue was disrupted using metallic balls and TissueLyser at 30 mut/sec for 15 seconds and RNA was isolated using the RNeasy Midi Kit (75144, Qiagen). .. RNA quality was assessed using Nanodrop spectrometry and 1 μg of RNA was converted into cDNA using the PrimeScript RT reagent kit (Perfect Real Time) (RR037A, Takara) according to manufacturer’s instructions.

    QC/EQA/Proficiency:

    Article Title: Temporal and spatial characterization of nicotinic acetylcholine receptor expression in Lepeophtheirus salmonis.
    Article Snippet: tinic acetylcholine receptors (nAChRs) are key mediators of synaptic transmission in invertebrates and m ts of pesticide action.. In early life stages of marine Copepoda, such as the parasitic salmon louse (Lepeo us salmonis), the expression dynamics and tissue localization of nAChRs remain poorly characterized.. H vestigate the expression of nAChR‐related transcripts across the life cycle of L. salmonis, and spatial di n of expression in the early copepodid life stage.

    Isolation:

    Article Title: Prenatal stress reprograms placental signaling and opposes maternal immune activation effects in the fetal brain.
    Article Snippet: .. Mouse fetal brain and placenta RNA-sequencing RNA was isolated from whole fetal brains and placentas using the RNeasy Midi Kit (Qiagen) per manufacturer protocol with homogenization performed using a tissue tearor. .. RNA concentration, integrity, and purity were assessed using a NanoDrop spectrophotometer and Agilent 5400 Bioanalyzer, with all samples exhibiting RIN values ≥ 9.3.

    Article Title: Using a modular massively parallel reporter assay to discover context-dependent regulatory activity in type 2 diabetes-linked noncoding regions
    Article Snippet: For use with the LHCN-M2 skeletal muscle myocyte cell line, we ported the assembled MPRA block (fragment, barcode, promoter, and GFP) to a lentiviral transfer vector (a gift from Nadav Ahituv; Addgene #137725) via restriction cloning., For the second library, we electroporated 30 μg of plasmid into 30 million 832/13 cells. .. We lysed cells 24 h later with Buffer RLT Plus and isolated total RNA with the Qiagen RNeasy Midi Kit. .. For all samples generated through the use of the second set of MPRA libraries, we reverse transcribed 30 μg of DNase I-treated total RNA into cDNA with a GFP-specific primer containing a 6-bp UMI ( C), then constructed indexed sequencing libraries for both the cDNA and input (plasmid or gDNA) libraries using Illumina-compatible primers ( ).

    Article Title: Impaired erythroid maturation in murine embryos upon loss of the preeclampsia-associated serine protease prostasin Prostasin loss impairs erythropoiesis
    Article Snippet: .. For RNA extraction, the tissue was disrupted using metallic balls and TissueLyser at 30 mut/sec for 15 seconds and RNA was isolated using the RNeasy Midi Kit (75144, Qiagen). .. RNA quality was assessed using Nanodrop spectrometry and 1 μg of RNA was converted into cDNA using the PrimeScript RT reagent kit (Perfect Real Time) (RR037A, Takara) according to manufacturer’s instructions.

    Homogenization:

    Article Title: Prenatal stress reprograms placental signaling and opposes maternal immune activation effects in the fetal brain.
    Article Snippet: .. Mouse fetal brain and placenta RNA-sequencing RNA was isolated from whole fetal brains and placentas using the RNeasy Midi Kit (Qiagen) per manufacturer protocol with homogenization performed using a tissue tearor. .. RNA concentration, integrity, and purity were assessed using a NanoDrop spectrophotometer and Agilent 5400 Bioanalyzer, with all samples exhibiting RIN values ≥ 9.3.

    Tissue:

    Article Title: Prenatal stress reprograms placental signaling and opposes maternal immune activation effects in the fetal brain.
    Article Snippet: .. Mouse fetal brain and placenta RNA-sequencing RNA was isolated from whole fetal brains and placentas using the RNeasy Midi Kit (Qiagen) per manufacturer protocol with homogenization performed using a tissue tearor. .. RNA concentration, integrity, and purity were assessed using a NanoDrop spectrophotometer and Agilent 5400 Bioanalyzer, with all samples exhibiting RIN values ≥ 9.3.

    Article Title: Impaired erythroid maturation in murine embryos upon loss of the preeclampsia-associated serine protease prostasin Prostasin loss impairs erythropoiesis
    Article Snippet: .. For RNA extraction, the tissue was disrupted using metallic balls and TissueLyser at 30 mut/sec for 15 seconds and RNA was isolated using the RNeasy Midi Kit (75144, Qiagen). .. RNA quality was assessed using Nanodrop spectrometry and 1 μg of RNA was converted into cDNA using the PrimeScript RT reagent kit (Perfect Real Time) (RR037A, Takara) according to manufacturer’s instructions.



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    Image Search Results


    Expression of the metagene clusters in immunological cell types. (a) The 199 ProbeSets from Figure 1a were used to cluster 44 samples of isolated cells and tissues with immune-system-related functions that were profiled on Affymetrix U133A arrays by Su and colleagues [GEO:GSE1133] . In each case, two samples for the following cell/tissue types are presented from left to right: fetal liver (1,2), K-562 (3,4), whole blood (5,6), CD33 myeloid (7,8), CD14 monocytes (9,10), CD34 (11,12), B lymphoblasts (13,14), CD56 natural killer cells (15,16), CD4 T cells (17,18), CD8 T cells (19,20), MOLT-4 (21,22), Raji (23,24), HL-60 (25,26), Daudi (27,28), CD105 (29,30), CD71 (31,32), BDCA4 dendritic cells (33,34), CD19 B cells (35,36), thymus (37,38), tonsil (39,40), lymph node (41,42), bone marrow (43,44). Details about the respective samples are given in Additional data file . HCK, hemopoietic cell kinase; LCK, lymphocyte-specific kinase; MHC, major histocompatibility complex; STAT1, signal transducer and activator of transcription 1. (b) Representation of the seven metagenes that were derived from the 199 ProbeSets as in Figure 1b.

    Journal: Breast Cancer Research : BCR

    Article Title: T-cell metagene predicts a favorable prognosis in estrogen receptor-negative and HER2-positive breast cancers

    doi: 10.1186/bcr2234

    Figure Lengend Snippet: Expression of the metagene clusters in immunological cell types. (a) The 199 ProbeSets from Figure 1a were used to cluster 44 samples of isolated cells and tissues with immune-system-related functions that were profiled on Affymetrix U133A arrays by Su and colleagues [GEO:GSE1133] . In each case, two samples for the following cell/tissue types are presented from left to right: fetal liver (1,2), K-562 (3,4), whole blood (5,6), CD33 myeloid (7,8), CD14 monocytes (9,10), CD34 (11,12), B lymphoblasts (13,14), CD56 natural killer cells (15,16), CD4 T cells (17,18), CD8 T cells (19,20), MOLT-4 (21,22), Raji (23,24), HL-60 (25,26), Daudi (27,28), CD105 (29,30), CD71 (31,32), BDCA4 dendritic cells (33,34), CD19 B cells (35,36), thymus (37,38), tonsil (39,40), lymph node (41,42), bone marrow (43,44). Details about the respective samples are given in Additional data file . HCK, hemopoietic cell kinase; LCK, lymphocyte-specific kinase; MHC, major histocompatibility complex; STAT1, signal transducer and activator of transcription 1. (b) Representation of the seven metagenes that were derived from the 199 ProbeSets as in Figure 1b.

    Article Snippet: Affymetrix HG-U133A microarray data of 1,781 primary breast cancer samples from 12 datasets were included.

    Techniques: Expressing, Isolation, Derivative Assay