rneasy midi kit (Qiagen)
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Rneasy Midi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 5313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 5313 article reviews
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Modification:Article Title: RNA binding proteome profiling reveals RNA-dependent activation of oncogenic host signaling during KSHV infection Article Snippet: .. Purification:Article Title: Design and validation of a ribosome display library for synthetic nanobody selection Article Snippet: DNA (10 μg) was transcripted using the RiboMAXTM Large Scale RNA Production Systems (Cat. P1300, Promega). .. The product was purified using RNA Extraction:Article Title: Temporal and spatial characterization of nicotinic acetylcholine receptor expression in Lepeophtheirus salmonis. Article Snippet: tinic acetylcholine receptors (nAChRs) are key mediators of synaptic transmission in invertebrates and m ts of pesticide action.. In early life stages of marine Copepoda, such as the parasitic salmon louse (Lepeo us salmonis), the expression dynamics and tissue localization of nAChRs remain poorly characterized.. H vestigate the expression of nAChR‐related transcripts across the life cycle of L. salmonis, and spatial di n of expression in the early copepodid life stage. Article Title: Impaired erythroid maturation in murine embryos upon loss of the preeclampsia-associated serine protease prostasin Prostasin loss impairs erythropoiesis Article Snippet: .. For RNA extraction, the tissue was disrupted using metallic balls and TissueLyser at 30 mut/sec for 15 seconds and RNA was isolated using the QC/EQA/Proficiency:Article Title: Temporal and spatial characterization of nicotinic acetylcholine receptor expression in Lepeophtheirus salmonis. Article Snippet: tinic acetylcholine receptors (nAChRs) are key mediators of synaptic transmission in invertebrates and m ts of pesticide action.. In early life stages of marine Copepoda, such as the parasitic salmon louse (Lepeo us salmonis), the expression dynamics and tissue localization of nAChRs remain poorly characterized.. H vestigate the expression of nAChR‐related transcripts across the life cycle of L. salmonis, and spatial di n of expression in the early copepodid life stage. Isolation:Article Title: Prenatal stress reprograms placental signaling and opposes maternal immune activation effects in the fetal brain. Article Snippet: .. Mouse fetal brain and placenta RNA-sequencing RNA was isolated from whole fetal brains and placentas using the Article Title: Using a modular massively parallel reporter assay to discover context-dependent regulatory activity in type 2 diabetes-linked noncoding regions Article Snippet: For use with the LHCN-M2 skeletal muscle myocyte cell line, we ported the assembled MPRA block (fragment, barcode, promoter, and GFP) to a lentiviral transfer vector (a gift from Nadav Ahituv; Addgene #137725) via restriction cloning., For the second library, we electroporated 30 μg of plasmid into 30 million 832/13 cells. .. We lysed cells 24 h later with Buffer RLT Plus and isolated total RNA with the Article Title: Impaired erythroid maturation in murine embryos upon loss of the preeclampsia-associated serine protease prostasin Prostasin loss impairs erythropoiesis Article Snippet: .. For RNA extraction, the tissue was disrupted using metallic balls and TissueLyser at 30 mut/sec for 15 seconds and RNA was isolated using the Homogenization:Article Title: Prenatal stress reprograms placental signaling and opposes maternal immune activation effects in the fetal brain. Article Snippet: .. Mouse fetal brain and placenta RNA-sequencing RNA was isolated from whole fetal brains and placentas using the Tissue:Article Title: Prenatal stress reprograms placental signaling and opposes maternal immune activation effects in the fetal brain. Article Snippet: .. Mouse fetal brain and placenta RNA-sequencing RNA was isolated from whole fetal brains and placentas using the Article Title: Impaired erythroid maturation in murine embryos upon loss of the preeclampsia-associated serine protease prostasin Prostasin loss impairs erythropoiesis Article Snippet: .. For RNA extraction, the tissue was disrupted using metallic balls and TissueLyser at 30 mut/sec for 15 seconds and RNA was isolated using the |
![Expression of the metagene clusters in immunological cell types. (a) The 199 ProbeSets from Figure 1a were used to cluster 44 samples of isolated cells and tissues with immune-system-related functions that were profiled on Affymetrix <t>U133A</t> arrays by Su and colleagues [GEO:GSE1133] . In each case, two samples for the following cell/tissue types are presented from left to right: fetal liver (1,2), K-562 (3,4), whole blood (5,6), CD33 myeloid (7,8), CD14 monocytes (9,10), CD34 (11,12), B lymphoblasts (13,14), CD56 natural killer cells (15,16), CD4 T cells (17,18), CD8 T cells (19,20), MOLT-4 (21,22), Raji (23,24), HL-60 (25,26), Daudi (27,28), CD105 (29,30), CD71 (31,32), BDCA4 dendritic cells (33,34), CD19 B cells (35,36), thymus (37,38), tonsil (39,40), lymph node (41,42), bone marrow (43,44). Details about the respective samples are given in Additional data file . HCK, hemopoietic cell kinase; LCK, lymphocyte-specific kinase; MHC, major histocompatibility complex; STAT1, signal transducer and activator of transcription 1. (b) Representation of the seven metagenes that were derived from the 199 ProbeSets as in Figure 1b.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8939/pmc02688939/pmc02688939__bcr2234-2.jpg)