Review



addgene 177765  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc addgene 177765
    Addgene 177765, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u1/CJ47+(U1+snRNA+(U1%3B21d)+3G+mutant)+(Plasmid+%2317776)/pm35013237-300-4-4
    Average 93 stars, based on 3 article reviews
    addgene 177765 - by Bioz Stars, 2026-11
    93/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Generation of bright monomeric red fluorescent proteins via computational design of enhanced chromophore packing
    Article Snippet: .. The plasmid containing the mSandy2 gene is available from Addgene (Plasmid #177760). ..

    Stable Transfection:

    Article Title: Grainyhead 1 acts as a drug-inducible conserved transcriptional regulator linked to insulin signaling and lifespan.
    Article Snippet: .. The resulting vector (pcDNA3.1_Hygro(+)_GRHL1-L3F6H, Addgene, #177765) was used to generate a HEK293 cell line stably overexpressing C-terminally 3xFLAG-6xHis tagged wild-type human GRHL1. .. For transient transfection of wild-type or mutated GRHL1 into the GRHL luciferase reporter cell line, individual amino acid codons in pcDNA3.1_Hygro(+)_GRHL1-L3F6H were mutated with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S) and primers selected with https://nebasechanger.neb.com/.

    Luciferase:

    Article Title: Grainyhead 1 acts as a drug-inducible conserved transcriptional regulator linked to insulin signaling and lifespan.
    Article Snippet: .. The resulting vectors (PX459_GRHL1-exon5, Addgene, #177763, and PX459_GRHL1-exon8, Addgene, #177764) were co-transfected into NATURE COMMUNICATIONS | (2022) 13:107 | https://doi.org/10.1038/s41467-021-27732-4 | www.nature.com/naturecommunications 11 a HEK293 GRHL luciferase reporter cell line to obtain the respective reporter with a concomitant GRHL1 loss-of-function mutation. ..

    Mutagenesis:

    Article Title: Grainyhead 1 acts as a drug-inducible conserved transcriptional regulator linked to insulin signaling and lifespan.
    Article Snippet: .. The resulting vectors (PX459_GRHL1-exon5, Addgene, #177763, and PX459_GRHL1-exon8, Addgene, #177764) were co-transfected into NATURE COMMUNICATIONS | (2022) 13:107 | https://doi.org/10.1038/s41467-021-27732-4 | www.nature.com/naturecommunications 11 a HEK293 GRHL luciferase reporter cell line to obtain the respective reporter with a concomitant GRHL1 loss-of-function mutation. ..



    Similar Products

    u1-snrnp 68/70 kda  (Surmodics IVD)
    90
    Surmodics IVD u1-snrnp 68/70 kda
    U1 Snrnp 68/70 Kda, supplied by Surmodics IVD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u1/U1-snRNP+68%2F70+kDa/custom%40a13000%4042664960
    Average 90 stars, based on 1 article reviews
    u1-snrnp 68/70 kda - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    mouse monoclonal anti u1 70k  (Synaptic Systems)
    94
    Synaptic Systems mouse monoclonal anti u1 70k
    Mouse Monoclonal Anti U1 70k, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u1/203+011/pmc13283296-1164-0-4
    Average 94 stars, based on 1 article reviews
    mouse monoclonal anti u1 70k - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    adaptive controller u1 t  (Kuang Lung Shing)
    86
    Kuang Lung Shing adaptive controller u1 t
    Adaptive Controller U1 T, supplied by Kuang Lung Shing, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u1/adaptive+controller+t+u1/pm41921428-157-2-30
    Average 86 stars, based on 1 article reviews
    adaptive controller u1 t - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier
    anti u1 70k  (ABclonal Biotechnology)
    94
    ABclonal Biotechnology anti u1 70k
    Anti U1 70k, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u1/SNRNP70+Rabbit+pAb/pmc13100119-294-10-14
    Average 94 stars, based on 1 article reviews
    anti u1 70k - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    gene exp cldn4 ss03375006 u1  (Thermo Fisher)
    94
    Thermo Fisher gene exp cldn4 ss03375006 u1
    Gene Exp Cldn4 Ss03375006 U1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u1/Gene+Exp%2E+CLDN4%2C+Ss03375006_u1/pmc12935204-436-25--1
    Average 94 stars, based on 1 article reviews
    gene exp cldn4 ss03375006 u1 - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    gene exp il10 ss03382372 u1  (Thermo Fisher)
    86
    Thermo Fisher gene exp il10 ss03382372 u1
    Gene Exp Il10 Ss03382372 U1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u1/Gene+Exp%2E+IL10%2C+Ss03382372_u1/pmc12935204-436-21--1
    Average 86 stars, based on 1 article reviews
    gene exp il10 ss03382372 u1 - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier
    gene exp cldn1 cf02713195 u1  (Thermo Fisher)
    87
    Thermo Fisher gene exp cldn1 cf02713195 u1
    Gene Exp Cldn1 Cf02713195 U1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u1/Gene+Exp%2E+CLDN1%2C+Cf02713195_u1/pm41678212-639-7--1
    Average 87 stars, based on 1 article reviews
    gene exp cldn1 cf02713195 u1 - by Bioz Stars, 2026-11
    87/100 stars
    		  Buy from Supplier
    gene exp fgf2 ss03375809 u1  (Thermo Fisher)
    85
    Thermo Fisher gene exp fgf2 ss03375809 u1
    RT-PCR analysis. Comparison of mRNA expression in the border and remote zones between the two groups using RT-PCR. (7A) SDF1, (7B) HGF, (7C) VEGFα, (7D) <t>FGF2,</t> (7E) TGFβ3, (7F) IL-10, (7G) PDGFRα, (7H) PDGFRβ. *p < 0.05, **p < 0.01.
    Gene Exp Fgf2 Ss03375809 U1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u1/Gene+Exp%2E+FGF2%2C+Ss03375809_u1/pmc12966441-212-19-10
    Average 85 stars, based on 1 article reviews
    gene exp fgf2 ss03375809 u1 - by Bioz Stars, 2026-11
    85/100 stars
    		  Buy from Supplier

    Image Search Results


    RT-PCR analysis. Comparison of mRNA expression in the border and remote zones between the two groups using RT-PCR. (7A) SDF1, (7B) HGF, (7C) VEGFα, (7D) FGF2, (7E) TGFβ3, (7F) IL-10, (7G) PDGFRα, (7H) PDGFRβ. *p < 0.05, **p < 0.01.

    Journal: Scientific Reports

    Article Title: Intravenous high mobility group box 1 fragment improves cardiac function, fibrosis, and coronary flow in porcine ischemic cardiomyopathy model

    doi: 10.1038/s41598-026-38592-7

    Figure Lengend Snippet: RT-PCR analysis. Comparison of mRNA expression in the border and remote zones between the two groups using RT-PCR. (7A) SDF1, (7B) HGF, (7C) VEGFα, (7D) FGF2, (7E) TGFβ3, (7F) IL-10, (7G) PDGFRα, (7H) PDGFRβ. *p < 0.05, **p < 0.01.

    Article Snippet: The following genes were analyzed using TaqMan Gene Expression Assay (Applied Biosystems): SDF1 (Ss03391855_m1), HGF (Ss06927387_m1), VEGFA (Ss03393990_m1), FGF2 (Ss03375809_u1), TGFβ3 (Ss03394351_m1), IL-10 (Ss03382372_u1), PDGFRα (Ss04955107_g1), and PDGFRβ (Ss06879561_m1).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Comparison, Expressing