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biacore  (Cytiva Europe)


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    Structured Review

    Cytiva Europe biacore
    Biacore, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 2933 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two-step+free+surface+detection+algorithm/Biacore/custom%40br110073%4025465760
    Average 99 stars, based on 2933 article reviews
    biacore - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    SPR Assay:

    Article Title: TopFor: Automated Preparation of AMBER-Compatible Parameters for Noncanonical Amino Acids in Peptides
    Article Snippet: .. Surface plasmon resonance (SPR) measurements were performed using a Biacore X100 system (GE Healthcare). ..

    Article Title: Physcion attenuates renal fibrosis by disrupting PKM2-FAK1 interaction and suppressing the FAK1/ERK signaling axis.
    Article Snippet: Background: Renal fibrosis (RF) is the final common pathway of chronic kidney disease with limited therapeutic options.. Pyruvate kinase M2 (PKM2) is upregulated in fibrotic kidneys and promotes pro-fibrotic signaling.. Physcion (PHY), a natural anthraquinone, exerts organ-protective effects, but its direct targets and systemic mechanisms in RF remain unclear.

    Article Title: Discovery of candidate antibodies and antigens for acute myeloid leukemia therapy by combining whole-cell phage display selection and CRISPR-Cas9 library screening
    Article Snippet: The absorbance was measured at 405 nm using a SpectraMax M5 microplate reader (Molecular Devices) and SoftMax Pro software (Molecular Devices). .. SPR experiments were conducted using a Biacore ×100 instrument along with Biacore reagents and evaluation software (Cytiva) to determine the kinetic and thermodynamic parameters of purified Fab-antigen interactions. .. A mouse anti-human IgG CH2 mAb was immobilized on a CM5 sensor chip according to the manufacturer’s instructions provided in the Human Antibody Capture Kit (Cytiva).

    Article Title: MHC restriction is structurally encoded by the T cell receptor germline
    Article Snippet: Subsequently, the proteins are biotinylated overnight and further purified by size-exclusion chromatography using a Superdex 200 column on an ÄKTA pure FPLC system (GE Healthcare). .. TCR affinity to the specific pMHC was measured by SPR on a Biacore T100 (GE Healthcare). .. The biotinylated single chaing trimer was immobilized on streptavidin chip (GE Healthcare) until 100-200 RU.

    Article Title: Unveiling structural determinants of peptide recognition by public and private SARS-CoV-2-specific T-cell receptors
    Article Snippet: For assay readout, 20 μL Steady-Glo Luciferase substrate (Promega) was added to eachwell and incubated at room temperature for 10min, and luminescence wasmeasured using a CLARIOstar plate reader in Firefly Green luciferase mode as a readout of T cell activation. .. SPR measurements were carried out on a Biacore S200 system (Cytiva). .. Biotinylated ORF3a(207-215)-HLA-A*01:01 complex was immobilised on a CAP sensor chip using the Biotin CAPture Kit (Cytiva), yielding 150-250 RU.

    Article Title: Balancing metabolism–control of weight gain, blood glucose levels and intestinal leakiness by alpha-lactalbumin-oleic acid complexes
    Article Snippet: .. Surface plasmon resonance analysis Real time biomolecular interaction analysis was performed by surface plasmon resonance technology using a Biacore X100 instrument (Cytiva Life Sciences) loaded with a Sensor Chip Ni-NTA (Cytiva Life Sciences, Cat# BR100034). ..

    Article Title: A small molecule inhibits Ebola virus entry through glycoprotein stabilization.
    Article Snippet: Pharmacokinetic parameters were calculated using a non-compartmental model (Phoenix WinNonlin, Pharsight Inc.). .. Surface plasmon resonance (SPR) was used to evaluate the binding of EBOV GP-targeting compounds to the EBOV GP ectodomain using a Biacore S200 system (Cytiva). .. Recombinant EBOVGP ectodomain was immobilized on a CM5 sensor chip (Cytiva) via chemical crosslinking.

    Binding Assay:

    Article Title: Physcion attenuates renal fibrosis by disrupting PKM2-FAK1 interaction and suppressing the FAK1/ERK signaling axis.
    Article Snippet: Background: Renal fibrosis (RF) is the final common pathway of chronic kidney disease with limited therapeutic options.. Pyruvate kinase M2 (PKM2) is upregulated in fibrotic kidneys and promotes pro-fibrotic signaling.. Physcion (PHY), a natural anthraquinone, exerts organ-protective effects, but its direct targets and systemic mechanisms in RF remain unclear.

    Article Title: A small molecule inhibits Ebola virus entry through glycoprotein stabilization.
    Article Snippet: Pharmacokinetic parameters were calculated using a non-compartmental model (Phoenix WinNonlin, Pharsight Inc.). .. Surface plasmon resonance (SPR) was used to evaluate the binding of EBOV GP-targeting compounds to the EBOV GP ectodomain using a Biacore S200 system (Cytiva). .. Recombinant EBOVGP ectodomain was immobilized on a CM5 sensor chip (Cytiva) via chemical crosslinking.

    Software:

    Article Title: Discovery of candidate antibodies and antigens for acute myeloid leukemia therapy by combining whole-cell phage display selection and CRISPR-Cas9 library screening
    Article Snippet: The absorbance was measured at 405 nm using a SpectraMax M5 microplate reader (Molecular Devices) and SoftMax Pro software (Molecular Devices). .. SPR experiments were conducted using a Biacore ×100 instrument along with Biacore reagents and evaluation software (Cytiva) to determine the kinetic and thermodynamic parameters of purified Fab-antigen interactions. .. A mouse anti-human IgG CH2 mAb was immobilized on a CM5 sensor chip according to the manufacturer’s instructions provided in the Human Antibody Capture Kit (Cytiva).

    Purification:

    Article Title: Discovery of candidate antibodies and antigens for acute myeloid leukemia therapy by combining whole-cell phage display selection and CRISPR-Cas9 library screening
    Article Snippet: The absorbance was measured at 405 nm using a SpectraMax M5 microplate reader (Molecular Devices) and SoftMax Pro software (Molecular Devices). .. SPR experiments were conducted using a Biacore ×100 instrument along with Biacore reagents and evaluation software (Cytiva) to determine the kinetic and thermodynamic parameters of purified Fab-antigen interactions. .. A mouse anti-human IgG CH2 mAb was immobilized on a CM5 sensor chip according to the manufacturer’s instructions provided in the Human Antibody Capture Kit (Cytiva).

    Analysis:

    Article Title: Balancing metabolism–control of weight gain, blood glucose levels and intestinal leakiness by alpha-lactalbumin-oleic acid complexes
    Article Snippet: .. Surface plasmon resonance analysis Real time biomolecular interaction analysis was performed by surface plasmon resonance technology using a Biacore X100 instrument (Cytiva Life Sciences) loaded with a Sensor Chip Ni-NTA (Cytiva Life Sciences, Cat# BR100034). ..



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