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BTX Harvard Apparatus two‐needle array electrode
Two‐Needle Array Electrode, supplied by BTX Harvard Apparatus, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-needle+array+electrodes/2+needle+array+electrode/pmc08981446-243-5-22
Average 90 stars, based on 1 article reviews
two‐needle array electrode - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Irreversible Electroporation-Induced Inflammation Facilitates Neutrophil-Mediated Drug Delivery to Enhance Pancreatic Cancer Therapy.
Article Snippet: Pancreatic cancer is a deadly disease with a five-year overall survival rate of around 11%.. Chemotherapy is a cornerstone in the treatment of this malignancy, but the intratumoral delivery of chemotherapy drugs is impaired by the highly fibrotic tumor-associated stroma.. Irreversible electroporation (IRE) is an ablative technique for treating locally advanced pancreatic cancer.

Article Title: Enhancing pancreatic cancer treatment: the role of H101 oncolytic virus in irreversible electroporation
Article Snippet: For in vivo experiments, electroporation was conducted using a 2-needle array electrode (BTX item #45–0168, BTX Harvard Apparatus, Holliston, MA) with a 5 mm gap.

Article Title: Simultaneous Delivery of Dual Inhibitors of DNA Damage Repair Sensitizes Pancreatic Cancer Response to Irreversible Electroporation.
Article Snippet: Pancreatic ductal adenocarcinoma (PDAC) is an abysmal disease refractory to most standard therapies.. Irreversible electroporation (IRE) is a local ablative technique for the clinical treatment of solid tumors, including locally advanced and unresectable PDAC, by intratumorally delivering high-intensity electric pulses to permanently disrupt cell membranes and induce cell death.. But the distribution of electric field is uneven within the tumor, and in some regions, tumor cells only experience temporary perturbation to their cell membrane, a phenomenon denoted as reversible electroporation (RE).

Sequencing:

Article Title: IFNα and 5-Aza-2’-deoxycytidine combined with a dendritic-cell targeting DNA vaccine alter tumor immune cell infiltration in the B16F10 melanoma model
Article Snippet: Vaccination-grade plasmids were extracted from E. coli DH5-α (InvitrogenTM ThermoFisher Scientific, Waltham, MA) using Qiagen ® (Germantown, MD) EndoFree ® Plasmid Kits and were diluted with endotoxin-free 1xPBS. .. Vaccine DNA preps were verified by insert sequencing (JHMI Synthesis and Sequencing Facility, Baltimore, MD), spectrophotometry, and gel electrophoresis, and then administered at 50 μg/dose into the gasctocnemius muscle followed by in vivo electroporation, pulsing the muscle with the ECM 830 Electro Square Porator with 2-Needle Array Electrode (BTX Harvard Apparatus; Holliston, MA) under the following parameters: 106 V; 20 ms pulse length; 200 ms pulse interval; 8 total pulses ( ). .. 50 μg ODN2395 Type C CpG (Innaxon LPS Biosciences, Tewkesbury, UK) was administered intramuscularly 2 days post-vaccination into vaccinated muscle.

Spectrophotometry:

Article Title: IFNα and 5-Aza-2’-deoxycytidine combined with a dendritic-cell targeting DNA vaccine alter tumor immune cell infiltration in the B16F10 melanoma model
Article Snippet: Vaccination-grade plasmids were extracted from E. coli DH5-α (InvitrogenTM ThermoFisher Scientific, Waltham, MA) using Qiagen ® (Germantown, MD) EndoFree ® Plasmid Kits and were diluted with endotoxin-free 1xPBS. .. Vaccine DNA preps were verified by insert sequencing (JHMI Synthesis and Sequencing Facility, Baltimore, MD), spectrophotometry, and gel electrophoresis, and then administered at 50 μg/dose into the gasctocnemius muscle followed by in vivo electroporation, pulsing the muscle with the ECM 830 Electro Square Porator with 2-Needle Array Electrode (BTX Harvard Apparatus; Holliston, MA) under the following parameters: 106 V; 20 ms pulse length; 200 ms pulse interval; 8 total pulses ( ). .. 50 μg ODN2395 Type C CpG (Innaxon LPS Biosciences, Tewkesbury, UK) was administered intramuscularly 2 days post-vaccination into vaccinated muscle.

Nucleic Acid Electrophoresis:

Article Title: IFNα and 5-Aza-2’-deoxycytidine combined with a dendritic-cell targeting DNA vaccine alter tumor immune cell infiltration in the B16F10 melanoma model
Article Snippet: Vaccination-grade plasmids were extracted from E. coli DH5-α (InvitrogenTM ThermoFisher Scientific, Waltham, MA) using Qiagen ® (Germantown, MD) EndoFree ® Plasmid Kits and were diluted with endotoxin-free 1xPBS. .. Vaccine DNA preps were verified by insert sequencing (JHMI Synthesis and Sequencing Facility, Baltimore, MD), spectrophotometry, and gel electrophoresis, and then administered at 50 μg/dose into the gasctocnemius muscle followed by in vivo electroporation, pulsing the muscle with the ECM 830 Electro Square Porator with 2-Needle Array Electrode (BTX Harvard Apparatus; Holliston, MA) under the following parameters: 106 V; 20 ms pulse length; 200 ms pulse interval; 8 total pulses ( ). .. 50 μg ODN2395 Type C CpG (Innaxon LPS Biosciences, Tewkesbury, UK) was administered intramuscularly 2 days post-vaccination into vaccinated muscle.

In Vivo:

Article Title: IFNα and 5-Aza-2’-deoxycytidine combined with a dendritic-cell targeting DNA vaccine alter tumor immune cell infiltration in the B16F10 melanoma model
Article Snippet: Vaccination-grade plasmids were extracted from E. coli DH5-α (InvitrogenTM ThermoFisher Scientific, Waltham, MA) using Qiagen ® (Germantown, MD) EndoFree ® Plasmid Kits and were diluted with endotoxin-free 1xPBS. .. Vaccine DNA preps were verified by insert sequencing (JHMI Synthesis and Sequencing Facility, Baltimore, MD), spectrophotometry, and gel electrophoresis, and then administered at 50 μg/dose into the gasctocnemius muscle followed by in vivo electroporation, pulsing the muscle with the ECM 830 Electro Square Porator with 2-Needle Array Electrode (BTX Harvard Apparatus; Holliston, MA) under the following parameters: 106 V; 20 ms pulse length; 200 ms pulse interval; 8 total pulses ( ). .. 50 μg ODN2395 Type C CpG (Innaxon LPS Biosciences, Tewkesbury, UK) was administered intramuscularly 2 days post-vaccination into vaccinated muscle.

Article Title: Vectors and vaccine cells for immunity against Zika virus
Article Snippet: Specifically, 50 micrograms of DNA containing expression vector that contains gp96-Ig and ZIKV antigens in 50 micro liters of saline was injected in the tibialis anterior muscle of anesthetized wild-type C57BL16 mice. .. A two-needle array electrode pair was inserted into muscle immediately after DNA delivery and the injection site was electroporated with field strength of 50 V/cm (constant) and six electric pulses of 50 ms each by using the AgilePulse In Vivo System (BTX, Harvard Apparatus). ..

Article Title: Vectors and vaccine cells for immunity against Zika virus
Article Snippet: In exemplary aspects, DNA (50 μg) containing expression vector that contains gp96-Ig and ZIKV antigens in 50 μL of saline is injected in the tibialis anterior muscle of anesthetized wild-type C57BL/6 mice. .. A two-needle array electrode pair is inserted into muscle immediately after DNA delivery and the injection site is electroporated with field strength of 50 V/cm (constant) and six electric pulses of 50 ms each by using the AgilePulse in Vivo System (BTX, Harvard Apparatus). ..

Electroporation:

Article Title: IFNα and 5-Aza-2’-deoxycytidine combined with a dendritic-cell targeting DNA vaccine alter tumor immune cell infiltration in the B16F10 melanoma model
Article Snippet: Vaccination-grade plasmids were extracted from E. coli DH5-α (InvitrogenTM ThermoFisher Scientific, Waltham, MA) using Qiagen ® (Germantown, MD) EndoFree ® Plasmid Kits and were diluted with endotoxin-free 1xPBS. .. Vaccine DNA preps were verified by insert sequencing (JHMI Synthesis and Sequencing Facility, Baltimore, MD), spectrophotometry, and gel electrophoresis, and then administered at 50 μg/dose into the gasctocnemius muscle followed by in vivo electroporation, pulsing the muscle with the ECM 830 Electro Square Porator with 2-Needle Array Electrode (BTX Harvard Apparatus; Holliston, MA) under the following parameters: 106 V; 20 ms pulse length; 200 ms pulse interval; 8 total pulses ( ). .. 50 μg ODN2395 Type C CpG (Innaxon LPS Biosciences, Tewkesbury, UK) was administered intramuscularly 2 days post-vaccination into vaccinated muscle.

In Vitro:

Article Title: Effects of bipolar irreversible electroporation with different pulse durations in a prostate cancer mouse model
Article Snippet: All cells were cultured at 37 °C in a humidified environment containing 5% CO 2 and sub-cultured with 0.025% trypsin–EDTA (Life Technologies, Gaithersburg, MD, USA). .. An in vitro bipolar IRE treatment was performed on the PC-3 cells using a 2-needle array electrode made by stainless steel (BTX Harvard Apparatus, Holliston, MA, USA) with an electric pulse generator (ECM 830; BTX Harvard Apparatus). ..

Injection:

Article Title: A SARS-CoV-2 RBD vaccine fused to the chemokine MIP-3α elicits sustained murine antibody responses over 12 months and enhanced lung T-cell responses
Article Snippet: .. Immediately following injection, the muscle was pulsed using an ECM 830 Electro Square Porator with 2-Needle Array Electrode (BTX Harvard Apparatus, Holliston, MA, USA) under the following parameters: 106 V, 20 ms pulse length, 200 ms pulse interval, and eight total pulses. .. CpG type B (ODN1826) (InvivoGen, San Diego, CA, USA) was used as the adjuvant for IM immunization and was diluted in 1× endotoxin-free PBS at 1 mg/mL.

Article Title: Vectors and vaccine cells for immunity against Zika virus
Article Snippet: Specifically, 50 micrograms of DNA containing expression vector that contains gp96-Ig and ZIKV antigens in 50 micro liters of saline was injected in the tibialis anterior muscle of anesthetized wild-type C57BL16 mice. .. A two-needle array electrode pair was inserted into muscle immediately after DNA delivery and the injection site was electroporated with field strength of 50 V/cm (constant) and six electric pulses of 50 ms each by using the AgilePulse In Vivo System (BTX, Harvard Apparatus). ..

Article Title: Vectors and vaccine cells for immunity against Zika virus
Article Snippet: In exemplary aspects, DNA (50 μg) containing expression vector that contains gp96-Ig and ZIKV antigens in 50 μL of saline is injected in the tibialis anterior muscle of anesthetized wild-type C57BL/6 mice. .. A two-needle array electrode pair is inserted into muscle immediately after DNA delivery and the injection site is electroporated with field strength of 50 V/cm (constant) and six electric pulses of 50 ms each by using the AgilePulse in Vivo System (BTX, Harvard Apparatus). ..



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