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two step immunohistochemistry detection kit  (Boster Bio)


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    Structured Review

    Boster Bio two step immunohistochemistry detection kit
    Two Step Immunohistochemistry Detection Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+step+detection+kit/Anti-PTPN5+Antibody/pm40836347-47-94-98
    Average 95 stars, based on 126 article reviews
    two step immunohistochemistry detection kit - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Portal hypertension contributes to ascites formation via the Piezo1−nuclear factor kappa-B−aquaporin1 pathway in liver cirrhosis
    Article Snippet: .. The slides were incubated with primary antibodies at 4 °C overnight, and then incubated with secondary antibodies at room temperature for 30 min. Staining process was performed using two step immunohistochemistry test kit (BOSTER Biological Technology). ..

    Article Title: Synapses and dendritic spines are eliminated in the primary visual cortex of mice subjected to chronic intraocular pressure elevation
    Article Snippet: .. The retinas were permeabilized using 2% Triton X-100, followed by a 2-hour blocking step with 5% goat serum (Boster, Wuhan, China), and subsequently incubated overnight at 4°C with an anti-RNA binding protein with multiple splicing (RBPMS) antibody (rabbit, 1:100, ProteinTech, Rosemont, IL, USA, Cat# 15187-1-AP, RRID: AB_2238431). .. Following three washes, the retinas were subjected to a 2-hour incubation at room temperature with a goat anti-rabbit IgG secondary antibody labeled with Alexa Fluor 488 (1:500, Cell Signaling Technology, Danvers, MA, USA, Cat# 4412, RRID: AB_1904025).

    Staining:

    Article Title: Portal hypertension contributes to ascites formation via the Piezo1−nuclear factor kappa-B−aquaporin1 pathway in liver cirrhosis
    Article Snippet: .. The slides were incubated with primary antibodies at 4 °C overnight, and then incubated with secondary antibodies at room temperature for 30 min. Staining process was performed using two step immunohistochemistry test kit (BOSTER Biological Technology). ..

    Article Title: Targeting endoplasmic reticulum stress-induced CLGN resensitizes hepatocellular carcinoma to apoptosis: paeonol synergistically enhances efficacy by dual inhibition of CLGN and NF-κB
    Article Snippet: .. Immunohistochemical staining was performed via a two-step method with a human monoclonal anti-rabbit CLGN antibody (1:100, BOSTER), a KI-67 antibody (1:400, CST), and an NF-κB antibody (1:400, CST). ..

    Immunohistochemistry:

    Article Title: Portal hypertension contributes to ascites formation via the Piezo1−nuclear factor kappa-B−aquaporin1 pathway in liver cirrhosis
    Article Snippet: .. The slides were incubated with primary antibodies at 4 °C overnight, and then incubated with secondary antibodies at room temperature for 30 min. Staining process was performed using two step immunohistochemistry test kit (BOSTER Biological Technology). ..

    Article Title: Ursolic acid suppresses gastric cancer by targeting the miR-27a-3p/Wnt/β-catenin signaling axis
    Article Snippet: Western blot , Cell lysates , SDS-PAGE, PVDF transfer, block 5% milk, primary antibody O/N, secondary 2 h , Bio-Rad ChemiDOC , Antibodies: Abclonal, Abcam, CST; Beyotime RIPA/BCA; Millipore ECL. .. Immunohistochemistry , TMA, 76 paired samples , Anti-DKK2 1:250; Rabbit Two-Step Kit + DAB , Optical microscope, 400 × , Abclonal; Zhong Shan-Golden Bridge, Beijing, China; BOSTER, USA. .. Xenograft , BALB/c nude mice, 4 wk , 5 × 106 AGS cells subcutaneously; UA 5/10 mg/kg i.p.; 3 groups, 5 mice , Tumor caliper , Shanghai Bikai, Shanghai, China; SPF conditions; Ethics approved.

    Immunofluorescence:

    Article Title: DNAH11 impairs memory via disrupted synaptic plasticity in noise-induced hidden hearing loss mice
    Article Snippet: .. Prior to immunofluorescence, the decalcified cochleae were exposed to a 1-h blocking step with 5% goat serum (AR0009, BOSTER, China). .. The tissues were then probed with a rabbit anti-CtBP2 (ab128871, Abcam, UK) in an overnight incubation at 4 °C.

    Article Title: DNAH11 impairs memory via disrupted synaptic plasticity in noise-induced hidden hearing loss mice.
    Article Snippet: .. Cochlear ribbon synapse Prior to immunofluorescence, the decalcified cochleae were exposed to a 1-hour blocking step with 5% goat serum (AR0009, BOSTER, China). .. The tissues were then probed with a rabbit anti-CtBP2 (ab128871, Abcam, UK) in an overnight incubation at 4°C.

    Blocking Assay:

    Article Title: DNAH11 impairs memory via disrupted synaptic plasticity in noise-induced hidden hearing loss mice
    Article Snippet: .. Prior to immunofluorescence, the decalcified cochleae were exposed to a 1-h blocking step with 5% goat serum (AR0009, BOSTER, China). .. The tissues were then probed with a rabbit anti-CtBP2 (ab128871, Abcam, UK) in an overnight incubation at 4 °C.

    Article Title: DNAH11 impairs memory via disrupted synaptic plasticity in noise-induced hidden hearing loss mice.
    Article Snippet: .. Cochlear ribbon synapse Prior to immunofluorescence, the decalcified cochleae were exposed to a 1-hour blocking step with 5% goat serum (AR0009, BOSTER, China). .. The tissues were then probed with a rabbit anti-CtBP2 (ab128871, Abcam, UK) in an overnight incubation at 4°C.

    Article Title: Synapses and dendritic spines are eliminated in the primary visual cortex of mice subjected to chronic intraocular pressure elevation
    Article Snippet: .. The retinas were permeabilized using 2% Triton X-100, followed by a 2-hour blocking step with 5% goat serum (Boster, Wuhan, China), and subsequently incubated overnight at 4°C with an anti-RNA binding protein with multiple splicing (RBPMS) antibody (rabbit, 1:100, ProteinTech, Rosemont, IL, USA, Cat# 15187-1-AP, RRID: AB_2238431). .. Following three washes, the retinas were subjected to a 2-hour incubation at room temperature with a goat anti-rabbit IgG secondary antibody labeled with Alexa Fluor 488 (1:500, Cell Signaling Technology, Danvers, MA, USA, Cat# 4412, RRID: AB_1904025).

    Microscopy:

    Article Title: Ursolic acid suppresses gastric cancer by targeting the miR-27a-3p/Wnt/β-catenin signaling axis
    Article Snippet: Western blot , Cell lysates , SDS-PAGE, PVDF transfer, block 5% milk, primary antibody O/N, secondary 2 h , Bio-Rad ChemiDOC , Antibodies: Abclonal, Abcam, CST; Beyotime RIPA/BCA; Millipore ECL. .. Immunohistochemistry , TMA, 76 paired samples , Anti-DKK2 1:250; Rabbit Two-Step Kit + DAB , Optical microscope, 400 × , Abclonal; Zhong Shan-Golden Bridge, Beijing, China; BOSTER, USA. .. Xenograft , BALB/c nude mice, 4 wk , 5 × 106 AGS cells subcutaneously; UA 5/10 mg/kg i.p.; 3 groups, 5 mice , Tumor caliper , Shanghai Bikai, Shanghai, China; SPF conditions; Ethics approved.

    Binding Assay:

    Article Title: Synapses and dendritic spines are eliminated in the primary visual cortex of mice subjected to chronic intraocular pressure elevation
    Article Snippet: .. The retinas were permeabilized using 2% Triton X-100, followed by a 2-hour blocking step with 5% goat serum (Boster, Wuhan, China), and subsequently incubated overnight at 4°C with an anti-RNA binding protein with multiple splicing (RBPMS) antibody (rabbit, 1:100, ProteinTech, Rosemont, IL, USA, Cat# 15187-1-AP, RRID: AB_2238431). .. Following three washes, the retinas were subjected to a 2-hour incubation at room temperature with a goat anti-rabbit IgG secondary antibody labeled with Alexa Fluor 488 (1:500, Cell Signaling Technology, Danvers, MA, USA, Cat# 4412, RRID: AB_1904025).

    Immunohistochemical staining:

    Article Title: Targeting endoplasmic reticulum stress-induced CLGN resensitizes hepatocellular carcinoma to apoptosis: paeonol synergistically enhances efficacy by dual inhibition of CLGN and NF-κB
    Article Snippet: .. Immunohistochemical staining was performed via a two-step method with a human monoclonal anti-rabbit CLGN antibody (1:100, BOSTER), a KI-67 antibody (1:400, CST), and an NF-κB antibody (1:400, CST). ..



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