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Yeasen Biotechnology tunel working solution
Tunel Working Solution, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tunel+solution/solution+tunel/pm42321826-83-5-8
Average 86 stars, based on 1 article reviews
tunel working solution - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Staining:

Article Title: Orientin Mitigates High Glucose/Ox‐LDL–Triggered Endothelial Cell Injury and Atherosclerosis by Regulating MARCH8‐Mediated NLRP3 Inflammasome Activation
Article Snippet: To assess cell apoptosis, HAECs were seeded in 96‐well plates (10,000 cells/well) overnight and then treated with Orientin (20 μM) in the presence or absence of ox‐LDL (50 mg/L, Mlbio) plus HG (30 mM of D‐glucose, Merck). .. After treatment for 24 h, cells were immobilized with 4% PFA for 10 min and stained with TUNEL solution (YEASEN). .. TUNEL‐positive cells were observed with a DMI4000B fluorescence microscopy (Leica).

Article Title: Orientin Mitigates High Glucose/Ox-LDL-Triggered Endothelial Cell Injury and Atherosclerosis by Regulating MARCH8-Mediated NLRP3 Inflammasome Activation.
Article Snippet: To assess cell apoptosis, HAECs were seeded in 96-well plates (10,000 cells/well) overnight and then treated with Orientin (20 μM) in the presence or absence of ox-LDL (50mg/L, Mlbio) plus HG (30mM of D-glucose, Merck). .. After treatment for 24 h, cells were immobilized with 4% PFA for 10min and stained with TUNEL solution (YEASEN). .. TUNEL-positive cells were observed with a DMI4000B fluorescence microscopy (Leica).

Article Title: Long noncoding RNA MCM3AP-AS1 attenuates sepsis-induced cardiomyopathy by improving inflammation, oxidative stress, and mitochondrial function through mediating the miR-501-3p/CADM1/STAT3 axis.
Article Snippet: Oxidative stress and inflammation are highly important for sepsis-mediated myocardial damage.. The long noncoding RNA (lncRNA) MCM3AP-AS1 is involved in inflammatory diseases, but its function in acute myocardial injury during sepsis has not been fully elucidated.. LPS and cecal ligation and puncture (CLP) were used to construct in vitro and in vivo sepsis-induced myocardial damage models, respectively. qRT-PCR was used to evaluate alterations in MCM3AP-AS1 and miR-501-3p alterations.

Article Title: ENO1 interacts with PKM to promote HBV-related hepatocellular carcinoma progression and HBV replication.
Article Snippet: After 48 hours of transfection, HepG2.2.15 cells were treated with 0.1% Triton X-100 (Yuanye Biotechnology, Guangzhou, China) for 20 minutes in the dark. .. Cells were then incubated with TUNEL working solution (Yeasen Biotechnology, Guangzhou, China) for 1 hour, followed by staining with 4',6-diamidino-2-phenylindole (DAPI) (Yuanye Biotechnology, Guangzhou, China) for 5 minutes at 37°C. .. Apoptotic cells were visualized and quantified under a fluorescence microscope (Olympus, Tokyo, Japan).

TUNEL Assay:

Article Title: Orientin Mitigates High Glucose/Ox‐LDL–Triggered Endothelial Cell Injury and Atherosclerosis by Regulating MARCH8‐Mediated NLRP3 Inflammasome Activation
Article Snippet: To assess cell apoptosis, HAECs were seeded in 96‐well plates (10,000 cells/well) overnight and then treated with Orientin (20 μM) in the presence or absence of ox‐LDL (50 mg/L, Mlbio) plus HG (30 mM of D‐glucose, Merck). .. After treatment for 24 h, cells were immobilized with 4% PFA for 10 min and stained with TUNEL solution (YEASEN). .. TUNEL‐positive cells were observed with a DMI4000B fluorescence microscopy (Leica).

Article Title: Orientin Mitigates High Glucose/Ox-LDL-Triggered Endothelial Cell Injury and Atherosclerosis by Regulating MARCH8-Mediated NLRP3 Inflammasome Activation.
Article Snippet: To assess cell apoptosis, HAECs were seeded in 96-well plates (10,000 cells/well) overnight and then treated with Orientin (20 μM) in the presence or absence of ox-LDL (50mg/L, Mlbio) plus HG (30mM of D-glucose, Merck). .. After treatment for 24 h, cells were immobilized with 4% PFA for 10min and stained with TUNEL solution (YEASEN). .. TUNEL-positive cells were observed with a DMI4000B fluorescence microscopy (Leica).

Article Title: Long noncoding RNA MCM3AP-AS1 attenuates sepsis-induced cardiomyopathy by improving inflammation, oxidative stress, and mitochondrial function through mediating the miR-501-3p/CADM1/STAT3 axis.
Article Snippet: Oxidative stress and inflammation are highly important for sepsis-mediated myocardial damage.. The long noncoding RNA (lncRNA) MCM3AP-AS1 is involved in inflammatory diseases, but its function in acute myocardial injury during sepsis has not been fully elucidated.. LPS and cecal ligation and puncture (CLP) were used to construct in vitro and in vivo sepsis-induced myocardial damage models, respectively. qRT-PCR was used to evaluate alterations in MCM3AP-AS1 and miR-501-3p alterations.

Article Title: Long noncoding RNA UCA1 knockdown inhibits cisplatin-resistant cervical cancer tumorigenesis via the miR-195-5p/IKBKB axis
Article Snippet: .. The sections were then treated with fluorescent TUNEL solution (reagent A:reagent B = 1:30; Yeasen, Shanghai, China) for 1 h at 37°C. ..

Article Title: ENO1 interacts with PKM to promote HBV-related hepatocellular carcinoma progression and HBV replication.
Article Snippet: After 48 hours of transfection, HepG2.2.15 cells were treated with 0.1% Triton X-100 (Yuanye Biotechnology, Guangzhou, China) for 20 minutes in the dark. .. Cells were then incubated with TUNEL working solution (Yeasen Biotechnology, Guangzhou, China) for 1 hour, followed by staining with 4',6-diamidino-2-phenylindole (DAPI) (Yuanye Biotechnology, Guangzhou, China) for 5 minutes at 37°C. .. Apoptotic cells were visualized and quantified under a fluorescence microscope (Olympus, Tokyo, Japan).

Incubation:

Article Title: Long noncoding RNA MCM3AP-AS1 attenuates sepsis-induced cardiomyopathy by improving inflammation, oxidative stress, and mitochondrial function through mediating the miR-501-3p/CADM1/STAT3 axis.
Article Snippet: Oxidative stress and inflammation are highly important for sepsis-mediated myocardial damage.. The long noncoding RNA (lncRNA) MCM3AP-AS1 is involved in inflammatory diseases, but its function in acute myocardial injury during sepsis has not been fully elucidated.. LPS and cecal ligation and puncture (CLP) were used to construct in vitro and in vivo sepsis-induced myocardial damage models, respectively. qRT-PCR was used to evaluate alterations in MCM3AP-AS1 and miR-501-3p alterations.

Article Title: ENO1 interacts with PKM to promote HBV-related hepatocellular carcinoma progression and HBV replication.
Article Snippet: After 48 hours of transfection, HepG2.2.15 cells were treated with 0.1% Triton X-100 (Yuanye Biotechnology, Guangzhou, China) for 20 minutes in the dark. .. Cells were then incubated with TUNEL working solution (Yeasen Biotechnology, Guangzhou, China) for 1 hour, followed by staining with 4',6-diamidino-2-phenylindole (DAPI) (Yuanye Biotechnology, Guangzhou, China) for 5 minutes at 37°C. .. Apoptotic cells were visualized and quantified under a fluorescence microscope (Olympus, Tokyo, Japan).



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