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tris buffered saline with tween20 ttbs  (Proteintech)


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    Structured Review

    Proteintech tris buffered saline with tween20 ttbs
    Tris Buffered Saline With Tween20 Ttbs, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1746 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ttbs+buffer/PRDM10+Antibody/pm40120859-42-5-34
    Average 96 stars, based on 1746 article reviews
    tris buffered saline with tween20 ttbs - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: A Blast Fungus Effector Targets a Tubby-like F-box Protein to Suppress Plant Immunity
    Article Snippet: .. For immunoprecipitation of GFP-tagged proteins 20 μl of GFP-Trap magnetic beads M-270 (ChromoTek) were washed 5 times with extraction buffer GTEN (25 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 10% v/v glycerol, and 0.1% Tween 20). ..

    Magnetic Beads:

    Article Title: A Blast Fungus Effector Targets a Tubby-like F-box Protein to Suppress Plant Immunity
    Article Snippet: .. For immunoprecipitation of GFP-tagged proteins 20 μl of GFP-Trap magnetic beads M-270 (ChromoTek) were washed 5 times with extraction buffer GTEN (25 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 10% v/v glycerol, and 0.1% Tween 20). ..

    Extraction:

    Article Title: A Blast Fungus Effector Targets a Tubby-like F-box Protein to Suppress Plant Immunity
    Article Snippet: .. For immunoprecipitation of GFP-tagged proteins 20 μl of GFP-Trap magnetic beads M-270 (ChromoTek) were washed 5 times with extraction buffer GTEN (25 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 10% v/v glycerol, and 0.1% Tween 20). ..

    Saline:

    Article Title: Tributyl phosphate induces liver aging via fumarate-mediated mitochondrial DNA escape triggered FPR2 activation.
    Article Snippet: Membranes were blocked with 5% non-fat milk in PBS-Tween-20 for 2 h, followed by incubation with primary antibodies against HP1 (Santa Cruz, USA), Lamin B1 (Santa Cruz, USA), Di/Tri-Methyl-Histone H3 (Lys9) (Santa Cruz, USA), p53 (Santa Cruz, USA), γ-H2A.X (Santa Cruz, USA), FPR2 (Proteintech, USA) GAPDH (Proteintech, USA), β-actin (Proteintech, USA), and α-tubulin (Proteintech, USA) overnight at 4◦C. .. After three washes with Tris-buffered saline containing Tween 20, membranes were incubated with corresponding secondary antibodies (Proteintech, USA). .. Protein bands were visualized using an enhanced chemiluminescence kit (YEASEN Biotechnology, China).

    Article Title: Integrative Single‐Cell and Bulk Transcriptomics Identifies a Proliferative Malignant State and Supports a Functional Contribution of TROAP in Osteosarcoma
    Article Snippet: Equal amounts of protein (30 μ g per lane) were separated by 10% SDS–PAGE and transferred to 0.45‐ μ m polyvinylidene difluoride membranes (Millipore, Billerica, Massachusetts, United States). .. Membranes were blocked for 1 h at room temperature with 5% nonfat milk in Tris‐buffered saline containing 0.1% Tween‐20 and incubated overnight at 4°C with anti‐TROAP (1:1000; cat. no. 13019‐1‐AP; Proteintech, Wuhan, China) or anti‐ β ‐actin (1:5000; cat. no. 66009‐1‐Ig; Proteintech). .. After washing, membranes were incubated for 1 h at room temperature with the appropriate horseradish‐peroxidase‐conjugated secondary antibody (1:5000; Cell Signaling Technology, Danvers, Massachusetts, United States).

    Article Title: PFOS increases cellular uric acid content and the abundance of URAT1 and GLUT9 in HK-2 cells.
    Article Snippet: Proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore). .. Membranes were blocked with 5% skimmed milk in Tris-buffered saline containing Tween-20 (TBST) for 1 h at room temperature and incubated overnight at 4 ◦C with antiGLUT9 (1:1000, Proteintech), anti-URAT1 (1:2000, Proteintech) or antiGAPDH (1:5000, Proteintech). .. After washing with TBST, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:10,000, Proteintech) for 1 h at room temperature.

    Article Title: TGF-β/Smad-Associated Epithelial-Fibroblast Remodeling Underlies Collagen Deposition in Chronic Rhinosinusitis With Nasal Polyps.
    Article Snippet: Equal amounts of protein were separated by 8%–10% Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred onto Polyvinylidene fluoride (PVDF) membranes (Millipore). .. After blocking with Bovine serum albumin (BSA) in Tris‐buffered saline with Tween‐20 (TBST), membranes were incubated overnight at 4°C with primary antibodies against COL1A1 (Affinity, AF7001, 1:500), POSTN (Affinity, DF6746, 1:500), N‐cadherin (Affinity, AF5239, 1:500), α‐SMA (Proteintech, 67735‐1‐Ig, 1:20000), E‐cadherin (Affinity, BF3043, 1:2000), phosphorylated Smad3 (Affinity, BF3080, 1:1000), Smad3 (Affinity, BF3079, 1:1000), and GAPDH (Proteintech, 60004‐1‐Ig, 1:10000), followed by incubation with HRP‐conjugated secondary antibodies (1:5000) at room temperature. .. Signals were developed with enhanced chemiluminescence reagent and captured using a ChemiScope 6100 imaging system (Clinx, Shanghai, China).

    Article Title: NRF2 activation attenuates obesity-induced adipose inflammation by suppressing STING signaling, with depot-specific regulation.
    Article Snippet: Equal amounts of protein were mixed with SDS-PAGE loading buffer (Solarbio, Cat. No. P1015) and denatured at 99 °C for 10 min. Proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Cat. No. ISEQ00010) at a constant current of 200 mA for 2 h. Membranes were blocked with 5% non-fat milk (BD Difco, Cat. No. 232100) or 5% BSA (Solarbio, Cat. No. A8020; for phospho-protein detection) at room temperature for 1 h and incubated with primary antibodies at 4 °C overnight. .. After washing with Tris-buffered saline containing 0.1% Tween-20 (TBST), membranes were incubated with horseradish peroxidase (HRP)-conjugated goat antimouse IgG (H + L) (Proteintech, Cat. No. SA00001-1) or HRP-conjugated goat anti-rabbit IgG (H + L) (Proteintech, Cat. No. SA00001-2) at a dilution of 1:5,000 for 1 h at room temperature. .. Protein bands were visualized using enhanced chemiluminescence (ECL) reagents (Biosharp, Cat. No. BL520A) and imaged using an Azure 600 Imaging System (Azure Biosystems).

    Incubation:

    Article Title: Tributyl phosphate induces liver aging via fumarate-mediated mitochondrial DNA escape triggered FPR2 activation.
    Article Snippet: Membranes were blocked with 5% non-fat milk in PBS-Tween-20 for 2 h, followed by incubation with primary antibodies against HP1 (Santa Cruz, USA), Lamin B1 (Santa Cruz, USA), Di/Tri-Methyl-Histone H3 (Lys9) (Santa Cruz, USA), p53 (Santa Cruz, USA), γ-H2A.X (Santa Cruz, USA), FPR2 (Proteintech, USA) GAPDH (Proteintech, USA), β-actin (Proteintech, USA), and α-tubulin (Proteintech, USA) overnight at 4◦C. .. After three washes with Tris-buffered saline containing Tween 20, membranes were incubated with corresponding secondary antibodies (Proteintech, USA). .. Protein bands were visualized using an enhanced chemiluminescence kit (YEASEN Biotechnology, China).

    Article Title: Humanin attenuates neuronal cellular senescence and preserves neurotransmitter release in differentiated PC12 cells.
    Article Snippet: Cellular senescence, characterized as a permanent arrest of cell proliferation, is triggered by various types of stress.. Senescent cells are identified by markers, including senescence-associated β-galactosidase (SA-β-gal) activity and the secretion of senescence-associated secretory phenotype (SASP) factors.. These senescent phenotypes have been observed in neurons, suggesting that cellular senescence may contribute to the pathogenesis of neurodegenerative diseases.

    Article Title: Integrative Single‐Cell and Bulk Transcriptomics Identifies a Proliferative Malignant State and Supports a Functional Contribution of TROAP in Osteosarcoma
    Article Snippet: Equal amounts of protein (30 μ g per lane) were separated by 10% SDS–PAGE and transferred to 0.45‐ μ m polyvinylidene difluoride membranes (Millipore, Billerica, Massachusetts, United States). .. Membranes were blocked for 1 h at room temperature with 5% nonfat milk in Tris‐buffered saline containing 0.1% Tween‐20 and incubated overnight at 4°C with anti‐TROAP (1:1000; cat. no. 13019‐1‐AP; Proteintech, Wuhan, China) or anti‐ β ‐actin (1:5000; cat. no. 66009‐1‐Ig; Proteintech). .. After washing, membranes were incubated for 1 h at room temperature with the appropriate horseradish‐peroxidase‐conjugated secondary antibody (1:5000; Cell Signaling Technology, Danvers, Massachusetts, United States).

    Article Title: PFOS increases cellular uric acid content and the abundance of URAT1 and GLUT9 in HK-2 cells.
    Article Snippet: Proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore). .. Membranes were blocked with 5% skimmed milk in Tris-buffered saline containing Tween-20 (TBST) for 1 h at room temperature and incubated overnight at 4 ◦C with antiGLUT9 (1:1000, Proteintech), anti-URAT1 (1:2000, Proteintech) or antiGAPDH (1:5000, Proteintech). .. After washing with TBST, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:10,000, Proteintech) for 1 h at room temperature.

    Article Title: TGF-β/Smad-Associated Epithelial-Fibroblast Remodeling Underlies Collagen Deposition in Chronic Rhinosinusitis With Nasal Polyps.
    Article Snippet: Equal amounts of protein were separated by 8%–10% Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred onto Polyvinylidene fluoride (PVDF) membranes (Millipore). .. After blocking with Bovine serum albumin (BSA) in Tris‐buffered saline with Tween‐20 (TBST), membranes were incubated overnight at 4°C with primary antibodies against COL1A1 (Affinity, AF7001, 1:500), POSTN (Affinity, DF6746, 1:500), N‐cadherin (Affinity, AF5239, 1:500), α‐SMA (Proteintech, 67735‐1‐Ig, 1:20000), E‐cadherin (Affinity, BF3043, 1:2000), phosphorylated Smad3 (Affinity, BF3080, 1:1000), Smad3 (Affinity, BF3079, 1:1000), and GAPDH (Proteintech, 60004‐1‐Ig, 1:10000), followed by incubation with HRP‐conjugated secondary antibodies (1:5000) at room temperature. .. Signals were developed with enhanced chemiluminescence reagent and captured using a ChemiScope 6100 imaging system (Clinx, Shanghai, China).

    Article Title: NRF2 activation attenuates obesity-induced adipose inflammation by suppressing STING signaling, with depot-specific regulation.
    Article Snippet: Equal amounts of protein were mixed with SDS-PAGE loading buffer (Solarbio, Cat. No. P1015) and denatured at 99 °C for 10 min. Proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Cat. No. ISEQ00010) at a constant current of 200 mA for 2 h. Membranes were blocked with 5% non-fat milk (BD Difco, Cat. No. 232100) or 5% BSA (Solarbio, Cat. No. A8020; for phospho-protein detection) at room temperature for 1 h and incubated with primary antibodies at 4 °C overnight. .. After washing with Tris-buffered saline containing 0.1% Tween-20 (TBST), membranes were incubated with horseradish peroxidase (HRP)-conjugated goat antimouse IgG (H + L) (Proteintech, Cat. No. SA00001-1) or HRP-conjugated goat anti-rabbit IgG (H + L) (Proteintech, Cat. No. SA00001-2) at a dilution of 1:5,000 for 1 h at room temperature. .. Protein bands were visualized using enhanced chemiluminescence (ECL) reagents (Biosharp, Cat. No. BL520A) and imaged using an Azure 600 Imaging System (Azure Biosystems).

    Transcranial Magnetic Stimulation:

    Article Title: Humanin attenuates neuronal cellular senescence and preserves neurotransmitter release in differentiated PC12 cells.
    Article Snippet: Cellular senescence, characterized as a permanent arrest of cell proliferation, is triggered by various types of stress.. Senescent cells are identified by markers, including senescence-associated β-galactosidase (SA-β-gal) activity and the secretion of senescence-associated secretory phenotype (SASP) factors.. These senescent phenotypes have been observed in neurons, suggesting that cellular senescence may contribute to the pathogenesis of neurodegenerative diseases.

    Cell-Signaling:

    Article Title: Humanin attenuates neuronal cellular senescence and preserves neurotransmitter release in differentiated PC12 cells.
    Article Snippet: Cellular senescence, characterized as a permanent arrest of cell proliferation, is triggered by various types of stress.. Senescent cells are identified by markers, including senescence-associated β-galactosidase (SA-β-gal) activity and the secretion of senescence-associated secretory phenotype (SASP) factors.. These senescent phenotypes have been observed in neurons, suggesting that cellular senescence may contribute to the pathogenesis of neurodegenerative diseases.

    Blocking Assay:

    Article Title: TGF-β/Smad-Associated Epithelial-Fibroblast Remodeling Underlies Collagen Deposition in Chronic Rhinosinusitis With Nasal Polyps.
    Article Snippet: Equal amounts of protein were separated by 8%–10% Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred onto Polyvinylidene fluoride (PVDF) membranes (Millipore). .. After blocking with Bovine serum albumin (BSA) in Tris‐buffered saline with Tween‐20 (TBST), membranes were incubated overnight at 4°C with primary antibodies against COL1A1 (Affinity, AF7001, 1:500), POSTN (Affinity, DF6746, 1:500), N‐cadherin (Affinity, AF5239, 1:500), α‐SMA (Proteintech, 67735‐1‐Ig, 1:20000), E‐cadherin (Affinity, BF3043, 1:2000), phosphorylated Smad3 (Affinity, BF3080, 1:1000), Smad3 (Affinity, BF3079, 1:1000), and GAPDH (Proteintech, 60004‐1‐Ig, 1:10000), followed by incubation with HRP‐conjugated secondary antibodies (1:5000) at room temperature. .. Signals were developed with enhanced chemiluminescence reagent and captured using a ChemiScope 6100 imaging system (Clinx, Shanghai, China).



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