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Structured Review

Nacalai trypsin edta solution
Trypsin Edta Solution, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypsin+solution/edta+trypsin/pm42268311-44-9-11
Average 86 stars, based on 1 article reviews
trypsin edta solution - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Saline:

Article Title: miR-136-5p Preferentially Suppresses Cancer Stem-like Cells in Pancreatic Cancer
Article Snippet: .. Cells were harvested with 0.25% trypsin–EDTA solution (Nacalai Tesque), washed with phosphate-buffered saline (PBS; Nacalai Tesque), and then resuspended in staining buffer (PBS containing 2% fetal bovine serum). ..

Article Title: Glypican-1-targeted antibody-drug conjugate shows therapeutic efficacy in lung squamous cell carcinoma.
Article Snippet: Background: Lung squamous cell carcinoma (LUSC) is the second most common subtype of lung cancer.. Although immune checkpoint inhibitors have improved outcomes, overall survival remains poor, and treatment responses vary markedly.. This study aimed to evaluate the preclinical efficacy of an antibody–drug conjugate (ADC)-targeting glypican-1 (GPC1), a cell-surface proteoglycan overexpressed in LUSC.

Staining:

Article Title: miR-136-5p Preferentially Suppresses Cancer Stem-like Cells in Pancreatic Cancer
Article Snippet: .. Cells were harvested with 0.25% trypsin–EDTA solution (Nacalai Tesque), washed with phosphate-buffered saline (PBS; Nacalai Tesque), and then resuspended in staining buffer (PBS containing 2% fetal bovine serum). ..

Incubation:

Article Title: OptoChaperoneA Biohybrid Tool for Regulating Protein Condensates in Cells and In Vitro
Article Snippet: .. Trypsin-EDTA solution (Cat No. 32777-44; Nacalai Tesque) diluted 0.025% (w/v) in PBS was added to detach the cells, followed by incubation for 5 min. Trypsin was neutralized with 8 mL of DMEM, and the suspension was centrifuged (100 rpm for 3 min). ..

Suspension:

Article Title: OptoChaperoneA Biohybrid Tool for Regulating Protein Condensates in Cells and In Vitro
Article Snippet: .. Trypsin-EDTA solution (Cat No. 32777-44; Nacalai Tesque) diluted 0.025% (w/v) in PBS was added to detach the cells, followed by incubation for 5 min. Trypsin was neutralized with 8 mL of DMEM, and the suspension was centrifuged (100 rpm for 3 min). ..

Single Cell:

Article Title: Low Ki-67 Marks Immunogenic CD8⁺ Tissue-resident Memory T Cell Enrichment in Vitiligo Skin
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

RNA Sequencing:

Article Title: Low Ki-67 Marks Immunogenic CD8⁺ Tissue-resident Memory T Cell Enrichment in Vitiligo Skin
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Modification:

Article Title: A natural coumarin, benjaminin, suppresses proliferation and migration and induces apoptosis in MCF-7 breast cancer cells.
Article Snippet: The human breast adenocarcinoma cell line, Michigan Cancer Foundation-7 (MCF-7), and the immortalized normal human mammary epithelial cell line, Michigan Cancer Foundation-10A (MCF-10A), were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. Dulbecco’s Modified Eagle Medium/Ham’s F-12 Nutrient Mixture (DMEM/F-12), 0.25% trypsin/EDTA, and hydrocortisone were obtained from Nacalai Tesque (Japan). .. Dulbecco’s Modified Eagle Medium (DMEM) and the Annexin V-FITC Apoptosis Detection Kit were obtained from Elabscience (USA).



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