Passaging:Article Title: Positive allosteric modulator activity of ginsenosides is restricted to P2X7 and P2X4 receptors
Article Snippet: Stable P2X-expressing cells were kept under geneticin (HelloBio) selection at 400 μg/ml. .. Passaging was performed twice weekly using 0.25% Trypsin–EDTA (Fisher Scientific). .. 96-well plates (catalogue number 10212811, ThermoFisher Scientific) were coated with 50 μg/mL Poly-D-Lysine (P6407, Sigma-Aldrich) and left for at least one hour before being washed with sterile water and allowed to dry.
Single Cell:Article Title: Scalable longitudinal imaging and transcriptomics of cells in dynamic enclosures
Article Snippet: .. A549 cells growing in culture were detached using Trypsin-EDTA (0.25%) (Fisher scientific) for 5 minutes at 37C to prepare a single-cell suspension, and labeled using Celltrace far red (ThermoFisher Scientific). ..
Suspension:Article Title: Scalable longitudinal imaging and transcriptomics of cells in dynamic enclosures
Article Snippet: .. A549 cells growing in culture were detached using Trypsin-EDTA (0.25%) (Fisher scientific) for 5 minutes at 37C to prepare a single-cell suspension, and labeled using Celltrace far red (ThermoFisher Scientific). ..
Labeling:Article Title: Scalable longitudinal imaging and transcriptomics of cells in dynamic enclosures
Article Snippet: .. A549 cells growing in culture were detached using Trypsin-EDTA (0.25%) (Fisher scientific) for 5 minutes at 37C to prepare a single-cell suspension, and labeled using Celltrace far red (ThermoFisher Scientific). ..
Cell Culture:Article Title: Bioresorbable, stereochemically defined elastomers and methods of making and using same
Article Snippet: Biological Reagents: Human mesenchymal stem cells (hMSCs) were ordered from Lonza and used at passage 4 following manufacturer protocols. .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEADTM assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITONTM X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlueTM metabolic assay were ordered from Life Technologies InvitrogenTM. .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEAD assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITON X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlue metabolic assay were ordered from Life Technologies Invitrogen.
Activity Assay:Article Title: Bioresorbable, stereochemically defined elastomers and methods of making and using same
Article Snippet: Biological Reagents: Human mesenchymal stem cells (hMSCs) were ordered from Lonza and used at passage 4 following manufacturer protocols. .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEADTM assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITONTM X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlueTM metabolic assay were ordered from Life Technologies InvitrogenTM. .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEAD assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITON X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlue metabolic assay were ordered from Life Technologies Invitrogen.
Saline:Article Title: Bioresorbable, stereochemically defined elastomers and methods of making and using same
Article Snippet: Biological Reagents: Human mesenchymal stem cells (hMSCs) were ordered from Lonza and used at passage 4 following manufacturer protocols. .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEADTM assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITONTM X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlueTM metabolic assay were ordered from Life Technologies InvitrogenTM. .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEAD assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITON X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlue metabolic assay were ordered from Life Technologies Invitrogen.
Staining:Article Title: Bioresorbable, stereochemically defined elastomers and methods of making and using same
Article Snippet: Biological Reagents: Human mesenchymal stem cells (hMSCs) were ordered from Lonza and used at passage 4 following manufacturer protocols. .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEADTM assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITONTM X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlueTM metabolic assay were ordered from Life Technologies InvitrogenTM. .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEAD assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITON X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlue metabolic assay were ordered from Life Technologies Invitrogen.
Metabolic Assay:Article Title: Bioresorbable, stereochemically defined elastomers and methods of making and using same
Article Snippet: Biological Reagents: Human mesenchymal stem cells (hMSCs) were ordered from Lonza and used at passage 4 following manufacturer protocols. .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEADTM assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITONTM X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlueTM metabolic assay were ordered from Life Technologies InvitrogenTM. .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEAD assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITON X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlue metabolic assay were ordered from Life Technologies Invitrogen.
other:
Incubation:Article Title: Spatial distribution of blood-brain barrier membrane proteins is controlled by sorting motifs and physiological signals in vivo
Article Snippet: .. For the anti-PGP antibody, the permeabilization step was substituted with a 3-minute antigen retrieval step using Trypsin-EDTA at room temperature (Fisher Scientific 25-200-114), PBS wash, brief (< 5 minute) incubation in Dulbecco‘s Modified Eagle Media (DMEM; Thermo Fisher 11995065) supplemented with 1% Penicillin/Streptomycin (Thermo Fisher 15140122) and 10% fetal bovine serum (R&D Systems S11150), and a final PBS wash prior to blocking. ..
Modification:Article Title: Spatial distribution of blood-brain barrier membrane proteins is controlled by sorting motifs and physiological signals in vivo
Article Snippet: .. For the anti-PGP antibody, the permeabilization step was substituted with a 3-minute antigen retrieval step using Trypsin-EDTA at room temperature (Fisher Scientific 25-200-114), PBS wash, brief (< 5 minute) incubation in Dulbecco‘s Modified Eagle Media (DMEM; Thermo Fisher 11995065) supplemented with 1% Penicillin/Streptomycin (Thermo Fisher 15140122) and 10% fetal bovine serum (R&D Systems S11150), and a final PBS wash prior to blocking. ..
Blocking Assay:Article Title: Spatial distribution of blood-brain barrier membrane proteins is controlled by sorting motifs and physiological signals in vivo
Article Snippet: .. For the anti-PGP antibody, the permeabilization step was substituted with a 3-minute antigen retrieval step using Trypsin-EDTA at room temperature (Fisher Scientific 25-200-114), PBS wash, brief (< 5 minute) incubation in Dulbecco‘s Modified Eagle Media (DMEM; Thermo Fisher 11995065) supplemented with 1% Penicillin/Streptomycin (Thermo Fisher 15140122) and 10% fetal bovine serum (R&D Systems S11150), and a final PBS wash prior to blocking. ..
FACS:Article Title: Hepatitis B virus-specific T cell responses
Article Snippet: After fixation, cells were suspended in permeabilization buffer (eBioscience, cat #: 00-8333-56). .. Starting on day two post-infection, a well each of HBV-infected and HBV-naïve primary hepatocytes was collected with 0.5% trypsin-EDTA (Fisher Scientific, cat #: SH30236.01) and washed twice in ice cold FACS buffer (PBS, Fisher Scientific, cat #:SH30256FS, with 10% fetal bovine serum). .. Primary hepatocytes were incubated for one hour at 4° C. with Hepatitis B Virus Core Antigen Antibody (clone: 13A9, Fisher Scientific, cat #: MA1-7606) conjugated to R-phycoerythrin (PE) using the Lightning-Link R-PE kit (Innova Biosciences, cat #: 703-0005).
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