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trypsin neutralizing solution  (PromoCell)


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    Structured Review

    PromoCell trypsin neutralizing solution
    Trypsin Neutralizing Solution, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trypsin+neutralization+solution/Trypsin+Neutralizing+Solution/pmc13017264-59-7-12
    Average 94 stars, based on 83 article reviews
    trypsin neutralizing solution - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma.
    Article Snippet: When confluent, RPE cells were passaged using 0.05% trypsin and neutralized by RPE medium, while NCMs were passaged using 0.025% trypsin and neutralized by Trypsin Neutralizing Solution (PromoCell).

    Article Title: Tunable organosilicon and silicon oxycarbide coatings via PECVD for vascular cell response control
    Article Snippet: A solution of 0.05% trypsin/ethylenediaminetetraacetic acid (EDTA)was used to detach theHUVECs,while PromoCell’s 0.04% trypsin/EDTA (C-41010) was used to detach the HCASMCs.

    Subculturing Assay:

    Article Title: IFITM1 inhibits Henipavirus membrane fusion by trapping ephrinB2 receptors in fusion-unfavorable membrane nanodomains
    Article Snippet: Human Umbilical Vein Endothelial Cells (HuVEC) (PromoCell, C-12203) were cultured in endothelial cell growth medium, which consists of SupplementMix (PromoCell, C-39215) and endothelial cell growth medium (PromoCell, C-22010B). .. HuVEC were treated with DetachKit-30 consisting of Hepes BSS (30 mM) (PromoCell, C-40000), Trypsin/EDTA (PromoCell, C-41000), and TNS (PromoCell, C-41100) before detaching and subculturing. .. To generate stable cell lines, retroviral pQCXIP vectors encoding FLAG-tagged IFITM constructs (6 μg), MLV-Gag/Pol (6 μg), and pmd2.G (3 μg) plasmids were co-transfected using polyethylenimine (PEI) at 1 mg/ml (Polysciences, 23966-100) to HEK293T cells cultured in a 10-cm dish.

    Article Title: Glycocalyx strengthens endothelial barrier function and protects from angioedema inducing compounds
    Article Snippet: .. The splitting reagents Hepes BSS, Trypsin/EDTA, and Trypsin Neutralizing Solution (all from PromoCell GmbH, Heidelberg ) were utilized for subculturing. .. The cell culture plates and filter inserts ( Sarstedt AG & Co. KG, Nümbrecht ) used in the experiments were pre-coated with a 50 μg/ml collagen I solution ( Corning GmbH, Kaiserslautern ).

    Incubation:

    Article Title: In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells.
    Article Snippet: .. After 2 h of incubation at 37 ◦C, the reaction was stopped with 2 mL of trypsin neutralization solution (TNS, PromoCell, Heidelberg, Germany). ..

    Neutralization:

    Article Title: In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells.
    Article Snippet: .. After 2 h of incubation at 37 ◦C, the reaction was stopped with 2 mL of trypsin neutralization solution (TNS, PromoCell, Heidelberg, Germany). ..

    Article Title: Highly solubilized and skin-permeable finasteride formulation using HPCD-incorporated chitosan nanocapsules to promote hair regeneration
    Article Snippet: Testosterone (TES) is converted to dihydrotestosterone (DHT), which can cause androgenic alopecia (AGA) because of an excessive reaction to TES.. A 5-α reductase inhibitor is required to alleviate or manage AGA, inhibiting the conversion of TES to DHT.. Finasteride (FINA) is a 5-α reductase inhibitor, FDA-approved for oral administration in alopecia treatment.

    Article Title: Cigarette smoking upregulates vascular expression of the novel atherosclerosis risk factor ADAMTS-7
    Article Snippet: Human Umbilical Vein Endothelial Cells (HUVECs) (C-12203; PromoCell) were cultured in endothelial cell growth media supplemented with 2% (v/v) FCS (C-22110; PromoCell). .. HUVECs were washed with HEPES-buffered saline solution (C-40020; PromoCell) and detached using trypsin-EDTA (0.04%/0.03%; C-41020; PromoCell), followed by neutralization with trypsin neutralizing solution (C-41120; PromoCell). .. The human monocyte cell line THP-1 (TIB-202; ATCC; Manassas, VA, USA) was cultured in RPMI 1640 Medium (A1049101; Thermo Fisher) supplemented with 10% fetal bovine serum (FBS; S0615; Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin-streptomycin (15140122; Thermo Fisher).

    Saline:

    Article Title: Cigarette smoking upregulates vascular expression of the novel atherosclerosis risk factor ADAMTS-7
    Article Snippet: Human Umbilical Vein Endothelial Cells (HUVECs) (C-12203; PromoCell) were cultured in endothelial cell growth media supplemented with 2% (v/v) FCS (C-22110; PromoCell). .. HUVECs were washed with HEPES-buffered saline solution (C-40020; PromoCell) and detached using trypsin-EDTA (0.04%/0.03%; C-41020; PromoCell), followed by neutralization with trypsin neutralizing solution (C-41120; PromoCell). .. The human monocyte cell line THP-1 (TIB-202; ATCC; Manassas, VA, USA) was cultured in RPMI 1640 Medium (A1049101; Thermo Fisher) supplemented with 10% fetal bovine serum (FBS; S0615; Sigma-Aldrich, St. Louis, MO, USA) and 1% penicillin-streptomycin (15140122; Thermo Fisher).



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