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trypsin neutralising solution  (PromoCell)


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    Structured Review

    PromoCell trypsin neutralising solution
    Trypsin Neutralising Solution, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trypsin+neutralization+solution/TNS%2C+Trypsin+Neutralizing+Solution/pm41390356-229-26-29
    Average 94 stars, based on 83 article reviews
    trypsin neutralising solution - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Single Cell:

    Article Title: Further characterisation of immortalised human lymphatic endothelial cells to explore their transcriptomic profile and VEGFC response
    Article Snippet: .. Single-cell suspensions were prepared from full-grown T75cm 2 flasks following trypsinization with detach kit (PromoCell) containing Trypsin 0.04%/EDTA 0.03% for 5 min at 37oC and inactivated with trypsin neutralising solution (PromoCell). .. Cells were blocked with 3% BSA/PBS and staining for flow cytometry analysis was performed at room temperature with the following fluorochrome conjugated primary antibodies prepared in 1% BSA/PBS for 30 min following two PBS (Ca 2+ Mg 2+ ) washing steps: APC anti-human VEGFR3 (FLT-4) Antibody [Clone: 9D9F9], Brilliant Violet 421 anti-human CD31 Antibody [Clone: WM59] and APC/Cyanine7 anti-Human Podoplanin Antibody [Clone: NC-08] purchased from BioLegend (San Diego, CA, USA) (Full list of antibodies in Supplementary Table 5).

    Article Title: Further characterisation of immortalised human lymphatic endothelial cells to explore their transcriptomic profile and VEGFC response.
    Article Snippet: .. Single-cell suspensions were prepared from full-grown T75cm2 flasks following trypsinization with detach kit (PromoCell) containing Trypsin 0.04%/EDTA 0.03% for 5 min at 37oC and inactivated with trypsin neutralising solution (PromoCell). .. Cells were blocked with 3% BSA/PBS and staining for flow cytometry analysis was performed at room temperature with the following fluorochrome conjugated primary antibodies prepared in 1% BSA/PBS for 30 minutes following two PBS (Ca2+ Mg2+) washing steps: APC anti-human VEGFR-3 (FLT-4) Antibody [Clone: 9D9F9], Brilliant Violet 421 anti-human CD31 Antibody [Clone: WM59] and APC/Cyanine7 anti-Human Podoplanin Antibody [Clone: NC-08] purchased from BioLegend (San Diego, CA, USA) (Full list of antibodies in Supplementary Table 5).

    Incubation:

    Article Title: In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells
    Article Snippet: .. After 2 h of incubation at 37 °C, the reaction was stopped with 2 mL of trypsin neutralization solution (TNS, PromoCell, Heidelberg, Germany). ..

    Neutralization:

    Article Title: In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells
    Article Snippet: .. After 2 h of incubation at 37 °C, the reaction was stopped with 2 mL of trypsin neutralization solution (TNS, PromoCell, Heidelberg, Germany). ..

    Produced:

    Article Title: SGLT2 inhibitors attenuate cholesterol accumulation in Human Aortic Smooth Muscle Cells.
    Article Snippet: Purpose: The influence of SGLT2 inhibitors (SGLT2i) on cholesterol accumulation in Human Aortic Smooth Muscle Cells (HAoSMCs) has not yet been evaluated.. This study aimed to assess the effect of SGLT2 inhibitors on cholesterol accumulation in HAoSMCs.. Materials and methods: HAoSMCs were treated with empagliflozin (1 and 10 μM), dapagliflozin (1 and 10 μM), and canagliflozin (1 and 10 μM), with or without cholesterol–methyl-β-cyclodextrin complex (cholesterol complex), for 144 h. Lipid accumulation was assessed using Oil Red O staining, and absorbance at 492 nm was measured to quantify lipid content.

    other:

    Article Title: Trypanosoma cruzi Dysregulates piRNAs Computationally Predicted to Target IL-6 Signaling Molecules During Early Infection of Primary Human Cardiac Fibroblasts
    Article Snippet: PHCF monolayers challenged with T. cruzi were washed 2X with Dulbecco’s phosphate-buffered saline, trypsinized using TripleE (Thermo Fisher Scientific), neutralized with TNS (PromoCell) and spun down at 1,300 rpm for 3 min.

    Centrifugation:

    Article Title: MHC class II proteins mediate sialic acid independent entry of human and avian H2N2 influenza A viruses.
    Article Snippet: Influenza A viruses (IAV) pose substantial burden on human and animal health.. Avian, swine and human IAV bind sialic acid on host glycans as receptor, whereas some bat IAV require MHC class II complexes for cell entry.. It is unknown how this difference evolved and whether dual receptor specificity is possible.

    FACS:

    Article Title: MHC class II proteins mediate sialic acid independent entry of human and avian H2N2 influenza A viruses.
    Article Snippet: Influenza A viruses (IAV) pose substantial burden on human and animal health.. Avian, swine and human IAV bind sialic acid on host glycans as receptor, whereas some bat IAV require MHC class II complexes for cell entry.. It is unknown how this difference evolved and whether dual receptor specificity is possible.



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