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truseq stranded mrna library prep kit  (Illumina Inc)


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    Structured Review

    Illumina Inc truseq stranded mrna library prep kit
    Truseq Stranded Mrna Library Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 14991 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/truseq/TruSeq+Stranded+mRNA+Library+Prep/pmc13049314-202-7-13
    Average 99 stars, based on 14991 article reviews
    truseq stranded mrna library prep kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Visual marker–assisted gene editing of MLO confers powdery mildew resistance in Nicotiana alata
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen, USA) following the manufacturer's protocol, with on-column DNase I digestion (Qiagen, Germany) to remove genomic DNA contamination, and RNA quality was assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA) for purity. .. Sequencing libraries were constructed using the TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) by enriching mRNA with oligo (dT) magnetic beads, fragmenting into 200–300 bp segments, reverse-transcribing into cDNA with random hexamers, and ligating with Illumina adapters; library quality was validated using the Agilent 2100 Bioanalyzer, followed by paired-end sequencing on the Illumina NovaSeq 6000 platform (Illumina, USA) at Tsingke Biotechnology (Kunming, China). ..

    Article Title: Therapeutic implications of targeting cancer testis antigen MAGEA1 in cervical cancer
    Article Snippet: Cellular RNA was isolated from shNC and shMAGEA1 #1 and #2 using TRIzol ® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to manufacturer's instructions. .. TruSeq Stranded mRNA Library Prep Kit (Illumina, Inc.) was used to isolate poly(A)+ mRNA and synthesize cDNA for sequencing library construction. .. The prepared libraries were sequenced in triplicate on the Illumina NovaSeq 6000 platform (Illumina, Inc.) with a paired-end 150 bp configuration.

    Article Title: Engineered atopic dermatitis models for recreating hypoxic conditions in atopic dermatitis microenvironments
    Article Snippet: .. Preprocessing was carried out using the TruSeq stranded mRNA Library Kit (Illumina), and the RNA sequence was aligned to the reference genome (GRCh38) using HISAT2 (version 2.2.1). ..

    Article Title: Oat grass improves meat tenderness and flavor, reduces fat deposition in small-tailed Han sheep
    Article Snippet: An Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) was used to assess RNA integrity, while a Nanodrop 2000 spectrophotometer (Thermo Scientific, MA, USA) was used to detect RNA concentration and purity. .. The TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) was used to prepare sequencing libraries in accordance with the manufacturer's instructions. .. OE Biotech Co., Ltd. (Shanghai, China) carried out transcriptome sequencing and the ensuing bioinformatic studies.

    Construct:

    Article Title: Visual marker–assisted gene editing of MLO confers powdery mildew resistance in Nicotiana alata
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen, USA) following the manufacturer's protocol, with on-column DNase I digestion (Qiagen, Germany) to remove genomic DNA contamination, and RNA quality was assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA) for purity. .. Sequencing libraries were constructed using the TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) by enriching mRNA with oligo (dT) magnetic beads, fragmenting into 200–300 bp segments, reverse-transcribing into cDNA with random hexamers, and ligating with Illumina adapters; library quality was validated using the Agilent 2100 Bioanalyzer, followed by paired-end sequencing on the Illumina NovaSeq 6000 platform (Illumina, USA) at Tsingke Biotechnology (Kunming, China). ..

    Article Title: Effects of dietary palygorskite supplementation on production performance and reproductive function of laying hens
    Article Snippet: Total RNA quality was assessed on agarose gels and quantified spectrophotometrically (NanoDrop ND-2000; Thermo Fisher Scientific, Waltham, MA, USA). .. The RNA-seq library was constructed using a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) and sequenced on an Illumina NovaSeq 6000 platform (Thermo Fisher Scientific, Shanghai, China). .. Adapter and low-quality sequences were removed from the raw data using FastQC, and clean reads were aligned to the Gallus gallus genome (GRCg6a) using Bowtie2/TopHat2.

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage
    Article Snippet: RNA integrity was analyzed by Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. The libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA). .. The transcriptome sequencing and analysis were conducted by Majorbio BioTech Co., Ltd. (Shanghai, China).

    Article Title: Small extracellular vesicles derived from hair follicle neural crest stem cells enhance perineurial cell proliferation and migration via the TGF-β/SMAD/HAS2 pathway
    Article Snippet: RNA integrity was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. Following the provided instructions, RNA libraries were constructed using a TruSeq stranded mRNA LT sample prep kit (Illumina, San Diego, CA, USA). .. The sequencing of the transcriptome and its subsequent analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China).

    Magnetic Beads:

    Article Title: Visual marker–assisted gene editing of MLO confers powdery mildew resistance in Nicotiana alata
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen, USA) following the manufacturer's protocol, with on-column DNase I digestion (Qiagen, Germany) to remove genomic DNA contamination, and RNA quality was assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA) for purity. .. Sequencing libraries were constructed using the TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) by enriching mRNA with oligo (dT) magnetic beads, fragmenting into 200–300 bp segments, reverse-transcribing into cDNA with random hexamers, and ligating with Illumina adapters; library quality was validated using the Agilent 2100 Bioanalyzer, followed by paired-end sequencing on the Illumina NovaSeq 6000 platform (Illumina, USA) at Tsingke Biotechnology (Kunming, China). ..

    RNA Sequencing:

    Article Title: Effects of dietary palygorskite supplementation on production performance and reproductive function of laying hens
    Article Snippet: Total RNA quality was assessed on agarose gels and quantified spectrophotometrically (NanoDrop ND-2000; Thermo Fisher Scientific, Waltham, MA, USA). .. The RNA-seq library was constructed using a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) and sequenced on an Illumina NovaSeq 6000 platform (Thermo Fisher Scientific, Shanghai, China). .. Adapter and low-quality sequences were removed from the raw data using FastQC, and clean reads were aligned to the Gallus gallus genome (GRCg6a) using Bowtie2/TopHat2.

    Sample Prep:

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage
    Article Snippet: RNA integrity was analyzed by Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. The libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA). .. The transcriptome sequencing and analysis were conducted by Majorbio BioTech Co., Ltd. (Shanghai, China).

    Article Title: Small extracellular vesicles derived from hair follicle neural crest stem cells enhance perineurial cell proliferation and migration via the TGF-β/SMAD/HAS2 pathway
    Article Snippet: RNA integrity was examined using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. Following the provided instructions, RNA libraries were constructed using a TruSeq stranded mRNA LT sample prep kit (Illumina, San Diego, CA, USA). .. The sequencing of the transcriptome and its subsequent analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China).

    Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis
    Article Snippet: The RNA concentration was measured with a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA), and RNA integrity was assessed with an Agilent 2100 bioanalyzer (Agilent Technology, USA). .. The TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) was used for library preparation according to the manufacturer's protocol. .. Transcriptomic sequencing and data analysis were performed by OE Biotech Co., Ltd. (Shanghai, China).

    Article Title: Oat grass improves meat tenderness and flavor, reduces fat deposition in small-tailed Han sheep
    Article Snippet: An Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) was used to assess RNA integrity, while a Nanodrop 2000 spectrophotometer (Thermo Scientific, MA, USA) was used to detect RNA concentration and purity. .. The TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) was used to prepare sequencing libraries in accordance with the manufacturer's instructions. .. OE Biotech Co., Ltd. (Shanghai, China) carried out transcriptome sequencing and the ensuing bioinformatic studies.



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    Validation of M2-exo targeting, Hp/IL-10 transfection expression, and Hp/Hb binding. (a) Fluorescence imaging showing cellular uptake of ICG and M2-exo@ICG by M1 microglia. (b,c) Flow cytometry and corresponding quantification of RhB and M2-exo@RhB internalized by M1 microglia (n = 3). (d,e) Schematic illustration and quantitative analysis of the in vitro phagocytosis-release kinetics of M2-exo@RhB in BV2 under ICH-mimicking stimulation (n = 6). (f) Fluorescence images showing Hp and IL-10 expression in M1 microglia treated with M2-exo@HI for 12, 24, 48, 72 h. (g) Mean fluorescence intensity (MFI) quantification of Hp and IL-10 expression (n = 3). (h,i) ELISA measurements of secreted Hp and IL-10 protein levels (n = 3). (j,k) qPCR analysis of relative Hp and IL-10 <t>mRNA</t> expression (n = 3). (l) Western blot detection of Hp and IL-10 protein expression. (m) Densitometric quantification of Hp and IL-10 protein levels from Western blot (n = 3). (n) Co-immunoprecipitation assay confirming the formation of Hp-Hb complex. Data are presented as mean ± SD. Statistical significance was calculated by unpaired Student's t -test (c and e), and one-way ANOVA with Tukey's multiple comparisons test (g-k and m).
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    Image Search Results


    Validation of M2-exo targeting, Hp/IL-10 transfection expression, and Hp/Hb binding. (a) Fluorescence imaging showing cellular uptake of ICG and M2-exo@ICG by M1 microglia. (b,c) Flow cytometry and corresponding quantification of RhB and M2-exo@RhB internalized by M1 microglia (n = 3). (d,e) Schematic illustration and quantitative analysis of the in vitro phagocytosis-release kinetics of M2-exo@RhB in BV2 under ICH-mimicking stimulation (n = 6). (f) Fluorescence images showing Hp and IL-10 expression in M1 microglia treated with M2-exo@HI for 12, 24, 48, 72 h. (g) Mean fluorescence intensity (MFI) quantification of Hp and IL-10 expression (n = 3). (h,i) ELISA measurements of secreted Hp and IL-10 protein levels (n = 3). (j,k) qPCR analysis of relative Hp and IL-10 mRNA expression (n = 3). (l) Western blot detection of Hp and IL-10 protein expression. (m) Densitometric quantification of Hp and IL-10 protein levels from Western blot (n = 3). (n) Co-immunoprecipitation assay confirming the formation of Hp-Hb complex. Data are presented as mean ± SD. Statistical significance was calculated by unpaired Student's t -test (c and e), and one-way ANOVA with Tukey's multiple comparisons test (g-k and m).

    Journal: Bioactive Materials

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage

    doi: 10.1016/j.bioactmat.2026.01.047

    Figure Lengend Snippet: Validation of M2-exo targeting, Hp/IL-10 transfection expression, and Hp/Hb binding. (a) Fluorescence imaging showing cellular uptake of ICG and M2-exo@ICG by M1 microglia. (b,c) Flow cytometry and corresponding quantification of RhB and M2-exo@RhB internalized by M1 microglia (n = 3). (d,e) Schematic illustration and quantitative analysis of the in vitro phagocytosis-release kinetics of M2-exo@RhB in BV2 under ICH-mimicking stimulation (n = 6). (f) Fluorescence images showing Hp and IL-10 expression in M1 microglia treated with M2-exo@HI for 12, 24, 48, 72 h. (g) Mean fluorescence intensity (MFI) quantification of Hp and IL-10 expression (n = 3). (h,i) ELISA measurements of secreted Hp and IL-10 protein levels (n = 3). (j,k) qPCR analysis of relative Hp and IL-10 mRNA expression (n = 3). (l) Western blot detection of Hp and IL-10 protein expression. (m) Densitometric quantification of Hp and IL-10 protein levels from Western blot (n = 3). (n) Co-immunoprecipitation assay confirming the formation of Hp-Hb complex. Data are presented as mean ± SD. Statistical significance was calculated by unpaired Student's t -test (c and e), and one-way ANOVA with Tukey's multiple comparisons test (g-k and m).

    Article Snippet: The libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA).

    Techniques: Biomarker Discovery, Transfection, Expressing, Binding Assay, Fluorescence, Imaging, Flow Cytometry, In Vitro, Enzyme-linked Immunosorbent Assay, Western Blot, Co-Immunoprecipitation Assay