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tris saline buffer  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology tris saline buffer
    Tris Saline Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tris-base+solution/Tris+Base+solution/pm15469997-83-9-23
    Average 95 stars, based on 371 article reviews
    tris saline buffer - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Helicobacter pylori is associated with less tumor-infiltrating lymphocytes and a poor prognosis in gastric cancer.
    Article Snippet: .. Heat-induced epitope retrieval was performed in a pH 9 Tris-based solution, and the slides were then incubated overnight at 4 °C with primary antibodies (anti-CD3: monoclonal mouse anti-human, Santa Cruz, Catalog #sc-1239; anti-CD8: monoclonal mouse anti-human, Santa Cruz, Catalog #sc-1181; antiPD-L1: monoclonal rabbit anti-human, Abcam, Catalog #ab205921). ..

    Article Title: Helicobacter pylori is associated with less tumor-infiltrating lymphocytes and a poor prognosis in gastric cancer
    Article Snippet: .. Heat-induced epitope retrieval was performed in a pH 9 Tris-based solution, and the slides were then incubated overnight at 4 °C with primary antibodies (anti-CD3: monoclonal mouse anti-human, Santa Cruz, Catalog #sc-1239; anti-CD8: monoclonal mouse anti-human, Santa Cruz, Catalog #sc-1181; anti-PD-L1: monoclonal rabbit anti-human, Abcam, Catalog #ab205921). ..

    Article Title: Regulation of Autonomic Motility in Human Gastric Muscle and the Gastroepiploic Artery by Stretch and pH
    Article Snippet: Equal amounts (20—40 μg) of soluble proteins were separated by 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis at 100 V for 90 min and transferred to polyvinylidene fluoride membranes at 0.25 A for 2 h in a Mini Trans-Blot Cell apparatus (Bio-Rad, Hercules, CA, USA) at 4 °C. .. The membranes were blocked overnight at 4 °C with 5% skim milk in TBS buffer solution (25 mM Tris–HCl (pH7.4) and 150 mM NaCl) under gentle shaking, then incubated with Kir 6.1, Kir 6.2, SUR1, SUR2A, or SUR2B (Santa Cruz Biotechnology, Inc., CA, USA; Millipore; Streess Marq) antibodies at dilutions of 1:500—1:200 in TBS buffer containing 1% skim milk at room temperature for another hour. .. After three washes with TBS buffer containing 0.1% Tween-20 (TBST), the membranes were treated with horseradish peroxidase-conjugated secondary antibodies (diluted 1:5000 in TBS with 1% skim milk) at room temperature for 1 h, followed by three washes in TBST. β-actin antibody (Abfrontier, 1:2000) and goat anti-rabbit IgG secondary antibody (Santa Cruz Biotechnology, Inc.) served as relative loading controls.

    Article Title: DYRK1A inhibition restores pancreatic functions and improves glucose metabolism in a preclinical model of type 2 diabetes
    Article Snippet: .. Rehydrated pancreatic sections were blocked for 1 h with 10% normal goat serum in Tris buffer saline and probed for insulin using primary mouse anti-insulin antibody (Santa Cruz Biotechnology, Heidelberg, Germany, ref. sc-8033) or mouse anti-glucagon antibody (Santa Cruz Biotechnology, Heidelberg, Germany, ref. sc-57171) for 1 h. Sections were then incubated with an HRP-conjugated anti-mouse secondary antibody (Jackson Laboratory, Ely, UK). .. The area of β and α cells were measured using an Olympus BX60 microscope equipped with Histolab 10.5.1 computer-assisted image analysis system software (Microvision Instrument, Evry, France).

    Article Title: Regulation of Autonomic Motility in Human Gastric Muscle and the Gastroepiploic Artery by Stretch and pH.
    Article Snippet: Equal amounts (20—40 μg) of soluble proteins were separated by 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis at 100 V for 90 min and transferred to polyvinylidene fluoride membranes at 0.25 A for 2 h in a Mini Trans-Blot Cell apparatus (Bio-Rad, Hercules, CA, USA) at 4 °C. .. The membranes were blocked overnight at 4 °C with 5% skim milk in TBS buffer solution (25 mM Tris–HCl (pH7.4) and 150 mM NaCl) under gentle shaking, then incubated with Kir 6.1, Kir 6.2, SUR1, SUR2A, or SUR2B (Santa Cruz Biotechnology, Inc., CA, USA; Millipore; Streess Marq) antibodies at dilutions of 1:500—1:200 in TBS buffer containing 1% skim milk at room temperature for another hour. .. After three washes with TBS buffer containing 0.1% Tween-20 (TBST), the membranes were treated with horseradish peroxidase-conjugated secondary antibodies (diluted 1:5000 in TBS with 1% skim milk) at room temperature for 1 h, followed by three washes in TBST. β-actin antibody (Abfrontier, 1:2000) and goat anti-rabbit IgG secondary antibody (Santa Cruz Biotechnology, Inc.) served as relative loading controls.

    Article Title: DYRK1A inhibition restores pancreatic functions and improves glucose metabolism in a preclinical model of type 2 diabetes.
    Article Snippet: .. Rehydrated pancreatic sections were blocked for 1 h with 10% normal goat serum in Tris buffer saline and probed for insulin using primary mouse anti-insulin antibody (Santa Cruz Biotechnology, Heidelberg, Germany, ref. sc-8033) or mouse anti-glucagon antibody (Santa Cruz Biotechnology, Heidelberg, Germany, ref. sc-57171) for 1 h. Sections were then incubated with an HRP-conjugated anti-mouse secondary antibody (Jackson Laboratory, Ely, UK). .. The area of β and cells were measured using an OLYMPUS BX60 microscope equipped with Histolab 10.5.1 computer-assisted image analysis system software (Microvision Instrument, Evry, France).

    Gentle:

    Article Title: Regulation of Autonomic Motility in Human Gastric Muscle and the Gastroepiploic Artery by Stretch and pH
    Article Snippet: Equal amounts (20—40 μg) of soluble proteins were separated by 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis at 100 V for 90 min and transferred to polyvinylidene fluoride membranes at 0.25 A for 2 h in a Mini Trans-Blot Cell apparatus (Bio-Rad, Hercules, CA, USA) at 4 °C. .. The membranes were blocked overnight at 4 °C with 5% skim milk in TBS buffer solution (25 mM Tris–HCl (pH7.4) and 150 mM NaCl) under gentle shaking, then incubated with Kir 6.1, Kir 6.2, SUR1, SUR2A, or SUR2B (Santa Cruz Biotechnology, Inc., CA, USA; Millipore; Streess Marq) antibodies at dilutions of 1:500—1:200 in TBS buffer containing 1% skim milk at room temperature for another hour. .. After three washes with TBS buffer containing 0.1% Tween-20 (TBST), the membranes were treated with horseradish peroxidase-conjugated secondary antibodies (diluted 1:5000 in TBS with 1% skim milk) at room temperature for 1 h, followed by three washes in TBST. β-actin antibody (Abfrontier, 1:2000) and goat anti-rabbit IgG secondary antibody (Santa Cruz Biotechnology, Inc.) served as relative loading controls.

    Article Title: Regulation of Autonomic Motility in Human Gastric Muscle and the Gastroepiploic Artery by Stretch and pH.
    Article Snippet: Equal amounts (20—40 μg) of soluble proteins were separated by 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis at 100 V for 90 min and transferred to polyvinylidene fluoride membranes at 0.25 A for 2 h in a Mini Trans-Blot Cell apparatus (Bio-Rad, Hercules, CA, USA) at 4 °C. .. The membranes were blocked overnight at 4 °C with 5% skim milk in TBS buffer solution (25 mM Tris–HCl (pH7.4) and 150 mM NaCl) under gentle shaking, then incubated with Kir 6.1, Kir 6.2, SUR1, SUR2A, or SUR2B (Santa Cruz Biotechnology, Inc., CA, USA; Millipore; Streess Marq) antibodies at dilutions of 1:500—1:200 in TBS buffer containing 1% skim milk at room temperature for another hour. .. After three washes with TBS buffer containing 0.1% Tween-20 (TBST), the membranes were treated with horseradish peroxidase-conjugated secondary antibodies (diluted 1:5000 in TBS with 1% skim milk) at room temperature for 1 h, followed by three washes in TBST. β-actin antibody (Abfrontier, 1:2000) and goat anti-rabbit IgG secondary antibody (Santa Cruz Biotechnology, Inc.) served as relative loading controls.

    SDS Page:

    Article Title: A novel CHD4-CTCF regulatory axis drives key developmental programs and transitions in male germ cells
    Article Snippet: The relative amount of protein was determined measuring absorbance at 260 nm using NanoDrop 2000c spectrophotometer (ThermoFisher Scientific). .. Proteins were solubilized with 2 X sample buffer (4 % SDS, 160 mM Tris-HCl, pH 6.8, 20 % glycerol, 4 % mM β-mercaptoethanol, and 0.005 % bromphenol blue) and 30 μg/lane of sample were separated by 4–15 % gradient Tris-acetate SDS-PAGE and electro transferred to PVDF membrane (Santa Cruz Biotechnology, sc-3723). ..

    Membrane:

    Article Title: A novel CHD4-CTCF regulatory axis drives key developmental programs and transitions in male germ cells
    Article Snippet: The relative amount of protein was determined measuring absorbance at 260 nm using NanoDrop 2000c spectrophotometer (ThermoFisher Scientific). .. Proteins were solubilized with 2 X sample buffer (4 % SDS, 160 mM Tris-HCl, pH 6.8, 20 % glycerol, 4 % mM β-mercaptoethanol, and 0.005 % bromphenol blue) and 30 μg/lane of sample were separated by 4–15 % gradient Tris-acetate SDS-PAGE and electro transferred to PVDF membrane (Santa Cruz Biotechnology, sc-3723). ..

    Saline:

    Article Title: DYRK1A inhibition restores pancreatic functions and improves glucose metabolism in a preclinical model of type 2 diabetes
    Article Snippet: .. Rehydrated pancreatic sections were blocked for 1 h with 10% normal goat serum in Tris buffer saline and probed for insulin using primary mouse anti-insulin antibody (Santa Cruz Biotechnology, Heidelberg, Germany, ref. sc-8033) or mouse anti-glucagon antibody (Santa Cruz Biotechnology, Heidelberg, Germany, ref. sc-57171) for 1 h. Sections were then incubated with an HRP-conjugated anti-mouse secondary antibody (Jackson Laboratory, Ely, UK). .. The area of β and α cells were measured using an Olympus BX60 microscope equipped with Histolab 10.5.1 computer-assisted image analysis system software (Microvision Instrument, Evry, France).

    Article Title: DYRK1A inhibition restores pancreatic functions and improves glucose metabolism in a preclinical model of type 2 diabetes.
    Article Snippet: .. Rehydrated pancreatic sections were blocked for 1 h with 10% normal goat serum in Tris buffer saline and probed for insulin using primary mouse anti-insulin antibody (Santa Cruz Biotechnology, Heidelberg, Germany, ref. sc-8033) or mouse anti-glucagon antibody (Santa Cruz Biotechnology, Heidelberg, Germany, ref. sc-57171) for 1 h. Sections were then incubated with an HRP-conjugated anti-mouse secondary antibody (Jackson Laboratory, Ely, UK). .. The area of β and cells were measured using an OLYMPUS BX60 microscope equipped with Histolab 10.5.1 computer-assisted image analysis system software (Microvision Instrument, Evry, France).



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