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tris based antigen retrieval buffer  (Vector Laboratories)


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    Vector Laboratories tris based antigen retrieval buffer
    Tris Based Antigen Retrieval Buffer, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 3165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tris+based+buffer/Antigen+Unmasking+Solution%2C+Tris-Based/pmc13058276-559-16-20
    Average 96 stars, based on 3165 article reviews
    tris based antigen retrieval buffer - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Multiplexed single-cell and spatial profiling reveal B cells and tertiary lymphoid structures as prognostic indicators in pleural mesothelioma.
    Article Snippet: .. Tissue sections were subjected to a two-step antigen retrieval procedure in citrate-based antigen unmasking solution at pH 6 (Vector Labs) followed by incubation in Tris-antigen retrieval solution (100mM Tris base, 10 mM EDTA, 0.5% Tween 20, pH 9). .. Slides were blocked with 10% donkey serum (Jackson Immuno) for 1 hour at RT, followed by autofluorescence quenching with a high-power LED (Samsung).

    Article Title: Piperlongumine suppresses YAP-ET-1-CXCL2 signaling to modulates aggressiveness of triple-negative breast cancer cells
    Article Snippet: Breast cancer tissue microarrays (TMAs; BR10010-L87) were purchased from US Biomax. .. The TMA was deparaffinized, rehydrated, and heated in an antigen-unmasking solution (Vector Laboratories) for 1 h. Slides were blocked with 3 % bovine serum albumin (BSA) and incubated overnight with anti-YAP anti-ET-1 primary antibodies at 4°C. .. Tissues were incubated with SignalStain Boost IHC detection reagent (Cell Signaling Technology) for 1 h at room temperature the next day.

    Tissue:

    Article Title: Pleuroparenchymal fibroelastosis in a monogenic mitochondrial DNA depletion syndrome.
    Article Snippet: Rehydration was done through a xylene (Carl Roth, Cat. CN80.1) and ethanol (Otto Fischar, Cat. 27690) series. .. Tissue decrosslinking was performed at 95°C in ddH2O with 1 % Antigen Unmasking Solution (Vector Laboratories, Cat. H3301) for 20 min. .. Slides were cooled down to RT for 20 min in 1X PBS (Chemsolute, Cat. 8418).

    Article Title: Photoreceptor replacement therapy using a tissue scaffold shows evidence of donor-host integration in non-human primate retina.
    Article Snippet: Rabbit anti-sox9 (1:800) Abcam Cat#AB185966; RRID: AB_2728660 Goat anti-NRL (1:300) R&D Systems Cat#AF2945SP; Lot#VYM0425061; RRID: AB_2155098 Goat anti-Chicken Alexa 546 (1:800) Thermo Fisher Scientific Cat#A-11040; RRID: AB_2534097 Donkey anti-Chicken Alexa 555 (1:800) Thermo Fisher Scientific Cat#A78949; RRID: AB_2921071 Donkey anti-Goat Alexa 488 (1:800) Molecular Probes Cat#A-11055; RRID: AB_2534102 Donkey anti-Goat Alexa 546 (1:800) Thermo Fisher Scientific Cat#A-11056; RRID: AB_2534103 Donkey anti-Guinea Pig Alexa 594 (1:500) Jackson ImmunoResearch Cat#706-585-148; RRID: AB_2340474 Donkey anti-Guinea Pig Alexa 647 (1:500) Jackson ImmunoResearch Cat#706-605-148; RRID: AB_2340476 Donkey anti-Mouse Alexa 488 (1:800) Molecular Probes Cat#A-21202; RRID: AB_141607 Donkey anti-Mouse Alexa 594 (1:800) Thermo Fisher Scientific Cat#A-21203; RRID: AB_2535789 Donkey anti-Mouse Alexa 594 (1:500) Jackson ImmunoResearch Cat#715-585-150; RRID: AB_2340854 Donkey anti-Mouse Alexa 647 (1:800) Molecular Probes Cat#A-31571; RRID: AB_162542 Goat anti-Mouse IgG1 Alexa 488 (1:800) Thermo Fisher Scientific Cat#A-21121; RRID: AB_2535764 Goat anti-Mouse IgG1 Alexa 594 (1:800) Molecular Probes Cat#A-21125; RRID: AB_141593 Goat anti-Mouse IgG1 Alexa 647 (1:800) Thermo Fisher Scientific Cat#A-21240; RRID: AB_2535809 Goat anti-Mouse IgG2a Alexa 488 (1:800) Molecular Probes Cat#A-21131; RRID: AB_141618 Goat anti-Mouse IgG2a Alexa 594 (1:800) Thermo Fisher Scientific Cat#A-21135; RRID: AB_2535774 Goat anti-Mouse IgG2a Alexa 647 (1:800) Thermo Fisher Scientific Cat#A-21241; RRID: AB_2535810 Donkey anti-Rabbit Alexa 488 (1:800) Thermo Fisher Scientific Cat#A-21206; RRID: AB_2535792 Donkey anti-Rabbit Alexa 488 (1:500) Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Donkey anti-Rabbit Alexa 546 (1:800) Thermo Fisher Scientific Cat#A-10040; RRID: AB_2534016 Donkey anti-Rabbit Alexa 594 (1:800) Thermo Fisher Scientific Cat#A-21207; RRID: AB_141637 Donkey anti-Rabbit Alexa 647 (1:800) Thermo Fisher Scientific Cat#A-31573; RRID: AB_2536183 .. Macaque eye University of Rochester N/A Human retinal tissue OHSU tissue bank N/A Chemicals, peptides, and recombinant proteins Hoechst Invitrogen Cat#33342 DAPI Fisher Scientific Cat#D21490 Unmasking solution Vector CatH-3301 Human Recombinant Laminin 521 BioLamina – 3D-RDM (3.5:1.5 DMEM:F12) Gibco – 2% B27 Supplement Gibco – 1× MEM NEAA Gibco – (Continued on next page) Stem Cell Reports | Vol. ..

    Recombinant:

    Article Title: Photoreceptor replacement therapy using a tissue scaffold shows evidence of donor-host integration in non-human primate retina.
    Article Snippet: Rabbit anti-sox9 (1:800) Abcam Cat#AB185966; RRID: AB_2728660 Goat anti-NRL (1:300) R&D Systems Cat#AF2945SP; Lot#VYM0425061; RRID: AB_2155098 Goat anti-Chicken Alexa 546 (1:800) Thermo Fisher Scientific Cat#A-11040; RRID: AB_2534097 Donkey anti-Chicken Alexa 555 (1:800) Thermo Fisher Scientific Cat#A78949; RRID: AB_2921071 Donkey anti-Goat Alexa 488 (1:800) Molecular Probes Cat#A-11055; RRID: AB_2534102 Donkey anti-Goat Alexa 546 (1:800) Thermo Fisher Scientific Cat#A-11056; RRID: AB_2534103 Donkey anti-Guinea Pig Alexa 594 (1:500) Jackson ImmunoResearch Cat#706-585-148; RRID: AB_2340474 Donkey anti-Guinea Pig Alexa 647 (1:500) Jackson ImmunoResearch Cat#706-605-148; RRID: AB_2340476 Donkey anti-Mouse Alexa 488 (1:800) Molecular Probes Cat#A-21202; RRID: AB_141607 Donkey anti-Mouse Alexa 594 (1:800) Thermo Fisher Scientific Cat#A-21203; RRID: AB_2535789 Donkey anti-Mouse Alexa 594 (1:500) Jackson ImmunoResearch Cat#715-585-150; RRID: AB_2340854 Donkey anti-Mouse Alexa 647 (1:800) Molecular Probes Cat#A-31571; RRID: AB_162542 Goat anti-Mouse IgG1 Alexa 488 (1:800) Thermo Fisher Scientific Cat#A-21121; RRID: AB_2535764 Goat anti-Mouse IgG1 Alexa 594 (1:800) Molecular Probes Cat#A-21125; RRID: AB_141593 Goat anti-Mouse IgG1 Alexa 647 (1:800) Thermo Fisher Scientific Cat#A-21240; RRID: AB_2535809 Goat anti-Mouse IgG2a Alexa 488 (1:800) Molecular Probes Cat#A-21131; RRID: AB_141618 Goat anti-Mouse IgG2a Alexa 594 (1:800) Thermo Fisher Scientific Cat#A-21135; RRID: AB_2535774 Goat anti-Mouse IgG2a Alexa 647 (1:800) Thermo Fisher Scientific Cat#A-21241; RRID: AB_2535810 Donkey anti-Rabbit Alexa 488 (1:800) Thermo Fisher Scientific Cat#A-21206; RRID: AB_2535792 Donkey anti-Rabbit Alexa 488 (1:500) Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Donkey anti-Rabbit Alexa 546 (1:800) Thermo Fisher Scientific Cat#A-10040; RRID: AB_2534016 Donkey anti-Rabbit Alexa 594 (1:800) Thermo Fisher Scientific Cat#A-21207; RRID: AB_141637 Donkey anti-Rabbit Alexa 647 (1:800) Thermo Fisher Scientific Cat#A-31573; RRID: AB_2536183 .. Macaque eye University of Rochester N/A Human retinal tissue OHSU tissue bank N/A Chemicals, peptides, and recombinant proteins Hoechst Invitrogen Cat#33342 DAPI Fisher Scientific Cat#D21490 Unmasking solution Vector CatH-3301 Human Recombinant Laminin 521 BioLamina – 3D-RDM (3.5:1.5 DMEM:F12) Gibco – 2% B27 Supplement Gibco – 1× MEM NEAA Gibco – (Continued on next page) Stem Cell Reports | Vol. ..

    Stem Cell:

    Article Title: Photoreceptor replacement therapy using a tissue scaffold shows evidence of donor-host integration in non-human primate retina.
    Article Snippet: Rabbit anti-sox9 (1:800) Abcam Cat#AB185966; RRID: AB_2728660 Goat anti-NRL (1:300) R&D Systems Cat#AF2945SP; Lot#VYM0425061; RRID: AB_2155098 Goat anti-Chicken Alexa 546 (1:800) Thermo Fisher Scientific Cat#A-11040; RRID: AB_2534097 Donkey anti-Chicken Alexa 555 (1:800) Thermo Fisher Scientific Cat#A78949; RRID: AB_2921071 Donkey anti-Goat Alexa 488 (1:800) Molecular Probes Cat#A-11055; RRID: AB_2534102 Donkey anti-Goat Alexa 546 (1:800) Thermo Fisher Scientific Cat#A-11056; RRID: AB_2534103 Donkey anti-Guinea Pig Alexa 594 (1:500) Jackson ImmunoResearch Cat#706-585-148; RRID: AB_2340474 Donkey anti-Guinea Pig Alexa 647 (1:500) Jackson ImmunoResearch Cat#706-605-148; RRID: AB_2340476 Donkey anti-Mouse Alexa 488 (1:800) Molecular Probes Cat#A-21202; RRID: AB_141607 Donkey anti-Mouse Alexa 594 (1:800) Thermo Fisher Scientific Cat#A-21203; RRID: AB_2535789 Donkey anti-Mouse Alexa 594 (1:500) Jackson ImmunoResearch Cat#715-585-150; RRID: AB_2340854 Donkey anti-Mouse Alexa 647 (1:800) Molecular Probes Cat#A-31571; RRID: AB_162542 Goat anti-Mouse IgG1 Alexa 488 (1:800) Thermo Fisher Scientific Cat#A-21121; RRID: AB_2535764 Goat anti-Mouse IgG1 Alexa 594 (1:800) Molecular Probes Cat#A-21125; RRID: AB_141593 Goat anti-Mouse IgG1 Alexa 647 (1:800) Thermo Fisher Scientific Cat#A-21240; RRID: AB_2535809 Goat anti-Mouse IgG2a Alexa 488 (1:800) Molecular Probes Cat#A-21131; RRID: AB_141618 Goat anti-Mouse IgG2a Alexa 594 (1:800) Thermo Fisher Scientific Cat#A-21135; RRID: AB_2535774 Goat anti-Mouse IgG2a Alexa 647 (1:800) Thermo Fisher Scientific Cat#A-21241; RRID: AB_2535810 Donkey anti-Rabbit Alexa 488 (1:800) Thermo Fisher Scientific Cat#A-21206; RRID: AB_2535792 Donkey anti-Rabbit Alexa 488 (1:500) Jackson ImmunoResearch Cat#711-545-152; RRID: AB_2313584 Donkey anti-Rabbit Alexa 546 (1:800) Thermo Fisher Scientific Cat#A-10040; RRID: AB_2534016 Donkey anti-Rabbit Alexa 594 (1:800) Thermo Fisher Scientific Cat#A-21207; RRID: AB_141637 Donkey anti-Rabbit Alexa 647 (1:800) Thermo Fisher Scientific Cat#A-31573; RRID: AB_2536183 .. Macaque eye University of Rochester N/A Human retinal tissue OHSU tissue bank N/A Chemicals, peptides, and recombinant proteins Hoechst Invitrogen Cat#33342 DAPI Fisher Scientific Cat#D21490 Unmasking solution Vector CatH-3301 Human Recombinant Laminin 521 BioLamina – 3D-RDM (3.5:1.5 DMEM:F12) Gibco – 2% B27 Supplement Gibco – 1× MEM NEAA Gibco – (Continued on next page) Stem Cell Reports | Vol. ..



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