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azide peg3 biotin  (Genesee Scientific)


Bioz Verified Symbol Genesee Scientific is a verified supplier
Bioz Manufacturer Symbol Genesee Scientific manufactures this product  
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    Structured Review

    Genesee Scientific azide peg3 biotin
    Azide Peg3 Biotin, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tris+base/Tris+Base/pmc09593816-357-5-17
    Average 93 stars, based on 12 article reviews
    azide peg3 biotin - by Bioz Stars, 2026-09
    93/100 stars

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    other:

    Article Title: FACT weakens the nucleosomal barrier to transcription and preserves its integrity by forming a hexasome-like intermediate.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Potassium chloride, KCl Sigma-Aldrich Cat# P9541 Proteinase K New England Biolabs Cat# P8107 Recombinant Albumin New England Biolabs Cat# B9200S Recombinant Human H2A The Histone Source, Colorado State University Cat# HH2A Recombinant Human H2B The Histone Source, Colorado State University Cat# HH2B Recombinant Human H3.3 The Histone Source, Colorado State University Cat# HH3_3 Recombinant Human H4 The Histone Source, Colorado State University Cat# HH4 Recombinant X. laevis H2A K119C The Histone Source, Colorado State University Cat# XH2A_K119C Recombinant X. laevis H2B The Histone Source, Colorado State University Cat# XH2B Recombinant X. laevis H3 The Histone Source, Colorado State University Cat# XH3 Recombinant X. laevis H4 The Histone Source, Colorado State University Cat# XH4 Spt4/5 Crickard et al.52 N/A Sodium Azide Sigma-Aldrich Cat# S2002 Sodium chloride, NaCl Sigma-Aldrich Cat# S9888 Streptavidin New England Biolabs Cat# N7021S T4 DNA Ligase New England Biolabs Cat# M0202 Tris Base Genesee Scientific Cat#18-146 Trolox Sigma-Aldrich Cat# 238813 YPD Millipore Sigma Cat# Y1375 Critical commercial assays QIAquick Gel Extraction Kit QIAGEN Cat# 28704 ZymoPURE II Plasmid Maxiprep Zymo Research Cat# D4202 Recombinant DNA Lambda DNA NEB Cat# N3011S Plasmid: pGEM-3z/12x601 This paper N/A Plasmid: pGEM-3Z/5S This paper N/A Plasmid: pGEM-3Z/601 Addgene Cat# 26656 Other Amicon Ultra-0.5 Centrifugal Filter Unit, 100K Millipore Sigma Cat# UFC510024 Amicon Ultra-0.5 Centrifugal Filter Unit, 10K Millipore Sigma Cat# UFC501008 Amicon Ultra-0.5 Centrifugal Filter Unit, 30K Millipore Sigma Cat# UFC503024 Carboxyl-functionalized polystyrene beads, 1 μm Bang Laboratories Cat# PC04N Hi-Trap Q HP columns Genesee Scientific Cat# 17-1153-01 Model 491 Prep Cell Bio-Rad Cat# 1702928 PES membrane filters Genesee Scientific Cat# 25-243 Steriflip Thermo Fisher Cat# SCGP00525 Streptavidin coated polystyrene beads, 1 μm Spherotech Cat# SVP-08-10 Superdex 200 Increase 10/300 GL Cytiva Cat# 28990944 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Cat# 10977-023 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 85, 1–13.e1–e8, June 5, 2025 e2

    Article Title: “SIT” with Emx1 ‐NuTRAP Mice: S imultaneous I NTACT and T RAP for Paired Transcriptomic and Epigenetic Sequencing
    Article Snippet: 250 μl 1 M Tris, pH 7.5 (from Tris base, molecular biology/proteomic grade, Genesee Scientific, cat. no. 18‐144) 600 μl 1 M MgCl 2 , molecular biology grade (Fisher Scientific, cat. no. AM9530G) 5 ml 3 M KCl, molecular biology grade (Alfa Aesar, cat. no. J64189, lot no. X18D048) 5 ml 10% IGEPAL CA‐630 (Sigma‐Aldrich, cat. no. I3021‐100ML) To 49.9 ml with HyPure Water, Molecular Biology Grade (GE Life Sciences, cat. no. SH30538.03) Store ≤1 week at 4°C Right before use, add 2 μl 1 M DTT, molecular biology grade (Sigma‐Aldrich, cat. no. D9779‐1G) per 1 ml buffer and 1 μl 100 μg/μl cycloheximide, 98% (Fisher Scientific, cat. no. NC9651091) per 1 ml buffer Once DTT and cycloheximide are added, store ≤8 hr on ice (4°C) Final concentrations: 0.05 M Tris, pH 7.5; 0.012 M MgCl 2 ; 0.3 M KCl; 1% IGEPAL CA‐630; 0.002 M DTT; and 100 μg/ml cycloheximide .

    Article Title: High-efficiency genomic mapping of chromatin-associated targets with CUT&RUN
    Article Snippet: Automated capillary electrophoresis consumables (e.g., Agilent High Sensitivity DNA Analysis Kit 5067-4626; or D1000 ScreenTape 5067-5582 and D1000 Reagents 5067-5583) CaCl 2 (e.g., Sigma-Aldrich C1016) Cell counter slides (e.g., Invitrogen C10228) Cell culture and appropriate growth medium ConA Beads ( e.g. , EpiCypher 21-1401) Control Antibodies IgG Negative Control ( e.g. , EpiCypher 13-0042) H3K4me3 Positive Control ( e.g. , EpiCypher 13-0060) H3K27me3 Positive Control ( e.g. , EpiCypher 13-0055) H3K27ac Positive Control*, labile PTM ( e.g. , EpiCypher 13-0059) Digitonin (5% stock in DMSO) ( e.g. , Millipore Sigma 300410) DMSO (e.g., Sigma-Aldrich D8418) DNA purification kit ( e.g. , EpiCypher 14-0052) E. coli Spike-in DNA ( e.g. , EpiCypher 18-1401) EDTA (0.5 M pH 8) ( e.g. , Sigma-Aldrich E5134) EGTA (0.5 M pH 8) ( e.g. , Sigma-Aldrich E3889) Formaldehyde* (37% w/v) ( e.g. , Sigma-Aldrich 252549) Fluorometer reagents ( e.g. , Invitrogen 1X dsDNA HS Kit Q33230) Glycerol ( e.g. , Sigma-Aldrich G5516) Glycine* (2.5M) ( e.g. , Sigma-Aldrich 50046) Glycogen (20 mg/mL) ( e.g. , Sigma-Aldrich, Roche 10930193001) HEPES (e.g., Sigma-Aldrich H3375) HEPES (0.5M, pH 7.9) [autoclaved or filter sterilized] [stocks for 2.3 ] HEPES (0.5M, pH 7.5) [autoclaved or filter sterilized] [stocks for 2.3 ] KCl (e.g., Sigma-Aldrich P3911) Library prep kit(s) ( e.g. , EpiCypher 14-1001 or 14-1002 [each provides different 48x multiplex barcode sets] or NEB NEBNext Ultra II DNA Library Kit E7645, E7600S, & E7780S) Low-retention filter pipette tips: any vendor MnCl 2 ( e.g. , Sigma-Aldrich 203734) Molecular biology grade water ( e.g. , Corning 46-000-CM) Multichannel reagent reservoirs: Thermo Fisher Scientific 14-387-072 NaCl ( e.g. , Sigma-Aldrich S5150-1L) Nuclei Extraction Buffer* ( e.g. , EpiCypher 21-1026) 100% Ethanol (200 proof) ( e.g. , Millipore Sigma EX0276) 1.5, 15, 50 mL tubes: any vendor 1X PBS* (calcium and magnesium free) ( e.g. , Cytiva SH30256.01) pAG-MNase ( e.g. , EpiCypher 15-1016) Propidium Iodide* ( e.g. , Thermo Fisher Scientific BMS500PI) Protease Inhibitor ( e.g. , Roche 11873580001) RNase A (10mg/mL) ( e.g. , Thermo Fisher Scientific EN0531) SDS (10% solution): any vendor SNAP-CUTANA Nucleosome Panels* [NOTE 1] Spermidine trihydrochloride (molecular weight 254.63) ( e.g. , Sigma-Aldrich S2501) SPRIselect Bead-Based Reagent (e.g., Beckman Coulter B23318) Test antibodies [NOTES 2-10] Tris Base ( e.g. , Genesee Scientific 18-145) Triton X-100 (10% solution) ( e.g. , Sigma-Aldrich T8787) Trypan Blue (0.4%) ( e.g. , Invitrogen T10282) Trypsin* (0.05%) ( e.g. , Cytiva SH30236.01) Tween 20 ( e.g. , VWR 9005-64-5) 0.2 mL 8-strip tubes with attached flat caps: EpiCypher 10-0009

    Article Title: A trailing ribosome speeds up RNA polymerase at the expense of transcript fidelity via force and allostery
    Article Snippet: Tris Base , Genesee Scientific , Cat#18-146; CAS: 77-86-1.

    Lysis:

    Article Title: Fenofibrate reduces glucose-induced barrier dysfunction in feline enteroids
    Article Snippet: .. Enteroid cells were lysed and protein extracted with lysis buffer (150 mM NaCl, 50 mM Tris base, 1% NP-40, 0.25% deoxycholic acid, 0.1% SDS) with 1% Prometheus General Protease Inhibitor Cocktail (Genesee Scientific). .. Protein was quantified via BCA Protein Assay kit (Thermo Fisher).

    Article Title: Fenofibrate reduces glucose-induced barrier dysfunction in feline enteroids.
    Article Snippet: .. Enteroid cells were lysed and protein extracted with lysis buffer (150 mM NaCl, 50 mM Tris base, 1% NP-40, 0.25% deoxycholic acid, 0.1% SDS) with 1% Prometheus General Protease Inhibitor Cocktail (Genesee Scientific). .. Protein was quantified via BCA Protein Assay kit (Thermo Fisher).

    Protease Inhibitor:

    Article Title: Fenofibrate reduces glucose-induced barrier dysfunction in feline enteroids
    Article Snippet: .. Enteroid cells were lysed and protein extracted with lysis buffer (150 mM NaCl, 50 mM Tris base, 1% NP-40, 0.25% deoxycholic acid, 0.1% SDS) with 1% Prometheus General Protease Inhibitor Cocktail (Genesee Scientific). .. Protein was quantified via BCA Protein Assay kit (Thermo Fisher).

    Article Title: Fenofibrate reduces glucose-induced barrier dysfunction in feline enteroids.
    Article Snippet: .. Enteroid cells were lysed and protein extracted with lysis buffer (150 mM NaCl, 50 mM Tris base, 1% NP-40, 0.25% deoxycholic acid, 0.1% SDS) with 1% Prometheus General Protease Inhibitor Cocktail (Genesee Scientific). .. Protein was quantified via BCA Protein Assay kit (Thermo Fisher).



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