constipation mouse model (MedChemExpress)
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MedChemExpress
constipation mouse model
Constipation Mouse Model, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trim21/TRIM21%2C+Mouse/pm41913080-49-4-17
Average 94 stars, based on 5 article reviews
Constipation Mouse Model, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trim21/TRIM21%2C+Mouse/pm41913080-49-4-17
Average 94 stars, based on 5 article reviews
constipation mouse model - by Bioz Stars,
2026-09
94/100 stars
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Derivative Assay:Article Title: Trim21 depletion alleviates bone loss in osteoporosis via activation of YAP1/β-catenin signaling Article Snippet: To mimic inflammation-induced bone loss in vitro, we seeded MC3T3-E1 cells into 6-well plates at a density of 2.5 × 10 5 for 24 h. Then, the cells were treated with different concentrations of LPS (Beyotime, Cat# S1732, China) or TNF-α (CST, Cat# 89025 C, USA) for another 24 h, followed by the analysis of Trim21, IL-6, and osteogenic markers (Runx2 and Osterix) by quantitative RT‒PCR or immunoblotting assays. .. BMMs were derived from 4- to 8-week-old Trim21 +/+ and Trim21 −/− mice or Article Title: Trim21 depletion alleviates bone loss in osteoporosis via activation of YAP1/β-catenin signaling. Article Snippet: To mimic inflammation-induced bone loss in vitro, we seeded MC3T3-E1 cells into 6-well plates at a density of 2.5 × 105 for 24 h. Then, the cells were treated with different concentrations of LPS (Beyotime, Cat# S1732, China) or TNF-α (CST, Cat# 89025 C, USA) for another 24 h, followed by the analysis of Trim21, IL-6, and osteogenic markers (Runx2 and Osterix) by quantitative RT‒PCR or immunoblotting assays. .. Osteoclastogenesis in BMMs and the coculture system BMMs were derived from 4- to 8-week-old Incubation:Article Title: Trim21 depletion alleviates bone loss in osteoporosis via activation of YAP1/β-catenin signaling Article Snippet: To mimic inflammation-induced bone loss in vitro, we seeded MC3T3-E1 cells into 6-well plates at a density of 2.5 × 10 5 for 24 h. Then, the cells were treated with different concentrations of LPS (Beyotime, Cat# S1732, China) or TNF-α (CST, Cat# 89025 C, USA) for another 24 h, followed by the analysis of Trim21, IL-6, and osteogenic markers (Runx2 and Osterix) by quantitative RT‒PCR or immunoblotting assays. .. BMMs were derived from 4- to 8-week-old Trim21 +/+ and Trim21 −/− mice or Article Title: Trim21 depletion alleviates bone loss in osteoporosis via activation of YAP1/β-catenin signaling. Article Snippet: To mimic inflammation-induced bone loss in vitro, we seeded MC3T3-E1 cells into 6-well plates at a density of 2.5 × 105 for 24 h. Then, the cells were treated with different concentrations of LPS (Beyotime, Cat# S1732, China) or TNF-α (CST, Cat# 89025 C, USA) for another 24 h, followed by the analysis of Trim21, IL-6, and osteogenic markers (Runx2 and Osterix) by quantitative RT‒PCR or immunoblotting assays. .. Osteoclastogenesis in BMMs and the coculture system BMMs were derived from 4- to 8-week-old other:Article Title: Macrophage TRIM21 Inhibition Ameliorates Murine Acute Pancreatitis via PHB2-Mediated Mitochondrial Stabilization. Article Snippet: TRIM21 was activated using Functional Assay:Article Title: Acacetin Alleviates Loperamide-Induced Functional Constipation by Inhibiting P53-Mediated Apoptosis in Colonic Epithelial Cells. Article Snippet: .. A loperamide- induced functional Suspension:Article Title: Acacetin Alleviates Loperamide-Induced Functional Constipation by Inhibiting P53-Mediated Apoptosis in Colonic Epithelial Cells. Article Snippet: .. A loperamide- induced functional |
![Tripartite motif-containing protein 21 (TRIM21) facilitates ubiquitination of carnitine palmitoyltransferase 1A (CPT1A) at lysine 161. (A) Reverse transcription polymerase chain reaction (RT-PCR) analysis of CPT1A with or without TRIM21 knockdown of KGN and COV434 cells ( n = 5). (B) Western blot (WB) analysis of CPT1A with TRIM21 overexpression in the presence of MG132 in KGN cells. (C) WB analysis of CPT1A in KGN cells treated with cycloheximide (CHX) and MG132 at different time points. (D) WB analysis of CPT1A in cells with or without TRIM21 silencing in CHX-chase experiment in KGN cells. (E and F) In KGN cells, coimmunoprecipitation (Co-IP) and WB analysis of CPT1A exogenous ubiquitination with TRIM21 knockdown or overexpression in the presence of MG132 (10 μM, 4 h). (G and H) In KGN cells, Co-IP and WB analysis of CPT1A endogenous ubiquitination with TRIM21 knockdown or overexpression in the presence of MG132 (10 μM, 4 h). (I and J) In KGN cells, Co-IP and WB analysis of exogenous ubiquitination of CPT1A treated with Flag-CPT1A and HA-UB (wild type [WT], K48-only, and K63-only) in the presence of MG132 (10 μM, 4 h). (K and L) Analysis of ubiquitination modification sites of CPT1A by immunoprecipitation-mass spectrometry (IP-MS). (M and N) In KGN cells, Co-IP and WB of exogenous ubiquitination of CPT1A treated with Flag-CPT1A mutant plasmids in the presence of MG132 (10 μM, 4 h). Data are expressed as means ± SD. IB, immunoblot.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7273/pmc13047273/pmc13047273__research.1223.fig.004.jpg)
