anti trim21 (Proteintech)
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Proteintech
anti trim21
Anti Trim21, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trim21/TRIM21+Antibody/pm41734032-148-14-16
Average 96 stars, based on 156 article reviews
Anti Trim21, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trim21/TRIM21+Antibody/pm41734032-148-14-16
Average 96 stars, based on 156 article reviews
anti trim21 - by Bioz Stars,
2026-11
96/100 stars
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Incubation:Article Title: TRIM21-dependent ubiquitination of MCCase induced by hsa_circ_0009061 drives H3K36 crotonylation to suppress epithelial ovarian cancer progression through regulating glycolysis Article Snippet: Proteins denatured at high temperature were separated via sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Millipore Corporation, Billerica, MA, USA). .. After being blocked with nonfat powdered milk at room temperature for 1.5 h, the PVDF membranes were incubated overnight at 4 °C with primary antibodies, including anti-MCCC1 (14861-1-AP, 1:1000, Proteintech), antiMCCC2(12117-1-AP, 1:1000, Proteintech), anti-DYKDDDDKtag (66008- 4-Ig, 1:2000, Proteintech), Article Title: TRIM21-Mediated ubiquitination of FBL suppresses PI3K/AKT signaling and tumor progression in clear cell renal cell carcinoma. Article Snippet: .. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated overnight at 4 ° C with the following primary antibodies: anti-FBL (Proteintech, Cat# 66985-1-Ig), anti-Trim21 (Proteintech, Cat# 12108-1-AP), Article Title: TRIM21-Mediated ubiquitination of FBL suppresses PI3K/AKT signaling and tumor progression in clear cell renal cell carcinoma Article Snippet: .. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-FBL (Proteintech, Cat# 66985-1-Ig), anti-Trim21 (Proteintech, Cat# 12108-1-AP), Cell-Signaling:Article Title: TRIM21-dependent ubiquitination of MCCase induced by hsa_circ_0009061 drives H3K36 crotonylation to suppress epithelial ovarian cancer progression through regulating glycolysis Article Snippet: Proteins denatured at high temperature were separated via sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Millipore Corporation, Billerica, MA, USA). .. After being blocked with nonfat powdered milk at room temperature for 1.5 h, the PVDF membranes were incubated overnight at 4 °C with primary antibodies, including anti-MCCC1 (14861-1-AP, 1:1000, Proteintech), antiMCCC2(12117-1-AP, 1:1000, Proteintech), anti-DYKDDDDKtag (66008- 4-Ig, 1:2000, Proteintech), Article Title: TRIM21-mediated OGT degradation inhibits breast cancer tumorigenesis and metastasis Article Snippet: .. The primary antibodies used were Article Title: RAD54L counteracts topoisomerase I cleavage complexes by stabilizing E3 ligase TRIM21 to maintain genome stability Article Snippet: Images were captured with a confocal microscope (ANDOR Technology). .. The primary antibodies used for immunofluorescence were goat anti-DDX4 (1:50 dilution, R&D systems AF2030), rabbit anti-FOXL2 (1:250 dilution, Abcam ab246511), rabbit anti-SOX9 (1:200 dilution, Millipore AB5535), goat anti-STELLA (1:50 dilution, R&D systems AF2566), rabbit anti-cleaved PARP1 (1:100 dilution, Cell Signaling Technology 94885), rabbit anti-Ki67 (1:200 dilution, Abcam ab15580), rabbit anti-Cyclin B1 (1:200 dilution, Cell Signaling Technology 4138), rabbit anti-53BP1 (1:200 dilution, Novus Biologicals NB100304), rabbit anti-γH2AX (1:50 dilution, Cell Signaling Technology 9718), rabbit anti-p-p53 (1:100 dilution, Cell Signaling Technology 12571), rabbit anti-RAD54L (1:200, Novus Biologicals NBP2-58260), mouse antiTOP1cc (1:100 dilution, Millipore MABE1084), other:Article Title: Environmental ciprofloxacin triggers pregnancy loss: senescence-driven miscarriage via TRIM21-mediated MFF degradation Article Snippet: Total proteins were extracted from human trophoblast HTR-8/SVneo cells, human villous tissues, and mouse placental tissues using RIPA lysis buffer (Thermo Fisher Scientific) and quantified using Pierce BCA Protein Assay Kit (Pierce). FLAG-tag:Article Title: Cryopreservation Disrupts TRIM21-Mediated AKAP3 Ubiquitination and Triggers Stress Granule Formation in Human Sperm. Article Snippet: he procedures for SDS–PAGE, transfer to PVDF membranes Millipore, MA, USA), blocking, and primary/secondary antiody incubation were performed as previously described [ 33 ]. .. he primary antibodies used in this study included: Flag tag ouse monoclonal antibody (66008-4-Ig, Proteintech), Flag tag abbit monoclonal antibody (80801-2-RR, Proteintech), GAPDH abbit polyclonal antibody (10494-1-AP, Proteintech), c-Myc tag ouse monoclonal antibody (60003-2-Ig, Proteintech), AKAP3 abbit polyclonal antibody (13907-1-AP, Proteintech), Ubiquitin Proteomics:Article Title: Cryopreservation Disrupts TRIM21-Mediated AKAP3 Ubiquitination and Triggers Stress Granule Formation in Human Sperm. Article Snippet: he procedures for SDS–PAGE, transfer to PVDF membranes Millipore, MA, USA), blocking, and primary/secondary antiody incubation were performed as previously described [ 33 ]. .. he primary antibodies used in this study included: Flag tag ouse monoclonal antibody (66008-4-Ig, Proteintech), Flag tag abbit monoclonal antibody (80801-2-RR, Proteintech), GAPDH abbit polyclonal antibody (10494-1-AP, Proteintech), c-Myc tag ouse monoclonal antibody (60003-2-Ig, Proteintech), AKAP3 abbit polyclonal antibody (13907-1-AP, Proteintech), Immunofluorescence:Article Title: RAD54L counteracts topoisomerase I cleavage complexes by stabilizing E3 ligase TRIM21 to maintain genome stability Article Snippet: Images were captured with a confocal microscope (ANDOR Technology). .. The primary antibodies used for immunofluorescence were goat anti-DDX4 (1:50 dilution, R&D systems AF2030), rabbit anti-FOXL2 (1:250 dilution, Abcam ab246511), rabbit anti-SOX9 (1:200 dilution, Millipore AB5535), goat anti-STELLA (1:50 dilution, R&D systems AF2566), rabbit anti-cleaved PARP1 (1:100 dilution, Cell Signaling Technology 94885), rabbit anti-Ki67 (1:200 dilution, Abcam ab15580), rabbit anti-Cyclin B1 (1:200 dilution, Cell Signaling Technology 4138), rabbit anti-53BP1 (1:200 dilution, Novus Biologicals NB100304), rabbit anti-γH2AX (1:50 dilution, Cell Signaling Technology 9718), rabbit anti-p-p53 (1:100 dilution, Cell Signaling Technology 12571), rabbit anti-RAD54L (1:200, Novus Biologicals NBP2-58260), mouse antiTOP1cc (1:100 dilution, Millipore MABE1084), |