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Servicebio Inc trap activity
Trap Activity, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trap/kit+staining+trap/pm42321919-150-13-16
Average 86 stars, based on 1 article reviews
trap activity - by Bioz Stars, 2026-11
86/100 stars

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Staining:

Article Title: Targeting the PARP10-BCAT2 axis disrupts branched-chain amino-acid metabolism to suppress bone metastasis in lung cancer.
Article Snippet: .. TRAP staining of osteoclasts in decalcified bone sections was performed by Wuhan Servicebio Technology Co., Ltd. (Wuhan, Hubei, China) following a standard automated histology protocol. ..

Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
Article Snippet: .. Sections were stained with hematoxylin and eosin (H&E) staining kit (#G1005, Servicebio, China). ..

Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
Article Snippet: .. After paraffin embedding and sectioning (5 μm), sections were stained with H&E (#G1005, Servicebio, China) or for tartrate-resistant acid phosphatase (TRAP) activity using a TRAP staining kit (#G1050, Servicebio, China). .. For immunofluorescence AR TIC LE IN PR ES S staining, sections were incubated with a rabbit monoclonal anti-COL1 antibody (1:200, #ab270993, Abcam) overnight at 4°C, followed by incubation with a goat anti-rabbit Alexa Fluor 555 secondary antibody (1:500, #A21428, Invitrogen) for 30 minutes at 37°C.

Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
Article Snippet: Nuclei were counterstained with DAPI (#D8200, Solarbio) for 10 minutes. .. Cells: Cells were fixed with 4% paraformaldehyde for 10 minutes and stained for TRAP activity (#G1050, Servicebio, China) according to the manufacturer's protocol. .. All stained sections and cells were imaged using a confocal laser scanning microscope (Olympus, Japan) or a light microscope.

Article Title: A New Wnt1 Mutant Rat Model of Osteogenesis Imperfecta and Its Application in AAV9‐Mediated Gene Therapy
Article Snippet: .. Sections were stained with hematoxylin and eosin (H&E), Masson′s trichrome, and a tartrate‐resistant Acid phosphatase (TRAP) commercial staining kit (TRAP Staining Kit, Servicebio, China). .. Stained sections were visualized with an optical microscope (NIKON ECLIPSE E100, Nikon, Japan).

Article Title: Phenylethanoid Glycosides from Cistanche tubulosa (Schenk) Wight Improve Radiation-Induced Bone Injury and Reduce Grem1 Expression in Osteoblasts.
Article Snippet: .. Freshly prepared TRAP incubation solution (Servicebio, G1050, Wuhan, China) was added, and sections were incubated at 37 ◦C for 20 min. After staining, nuclei were counterstained with hematoxylin for approximately 15 s, differentiated, and blued (sections were immersed into the reverse blue solution for 10 s, then removed, and washed with water). ..

Activity Assay:

Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
Article Snippet: .. After paraffin embedding and sectioning (5 μm), sections were stained with H&E (#G1005, Servicebio, China) or for tartrate-resistant acid phosphatase (TRAP) activity using a TRAP staining kit (#G1050, Servicebio, China). .. For immunofluorescence AR TIC LE IN PR ES S staining, sections were incubated with a rabbit monoclonal anti-COL1 antibody (1:200, #ab270993, Abcam) overnight at 4°C, followed by incubation with a goat anti-rabbit Alexa Fluor 555 secondary antibody (1:500, #A21428, Invitrogen) for 30 minutes at 37°C.

Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
Article Snippet: Nuclei were counterstained with DAPI (#D8200, Solarbio) for 10 minutes. .. Cells: Cells were fixed with 4% paraformaldehyde for 10 minutes and stained for TRAP activity (#G1050, Servicebio, China) according to the manufacturer's protocol. .. All stained sections and cells were imaged using a confocal laser scanning microscope (Olympus, Japan) or a light microscope.

other:

Article Title: Amuc_1473 Links Gut Microbes to Skeletal Homeostasis and Counteracts Multifactorial Osteoporosis.
Article Snippet: RAW264.7 cells (ATCC; ockville, USA) were cultured in high-glucose Dulbecco’s modfied Eagle’s medium (DMEM; Gibco) with 10% FBS and 1% PS.

Incubation:

Article Title: Phenylethanoid Glycosides from Cistanche tubulosa (Schenk) Wight Improve Radiation-Induced Bone Injury and Reduce Grem1 Expression in Osteoblasts.
Article Snippet: .. Freshly prepared TRAP incubation solution (Servicebio, G1050, Wuhan, China) was added, and sections were incubated at 37 ◦C for 20 min. After staining, nuclei were counterstained with hematoxylin for approximately 15 s, differentiated, and blued (sections were immersed into the reverse blue solution for 10 s, then removed, and washed with water). ..



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