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trap solution  (Nikon)


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    Structured Review

    Nikon trap solution
    Trap Solution, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+solution/ECLIPSE+Ts2R/pmc12655559-63-45-59
    Average 99 stars, based on 1626 article reviews
    trap solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Morphology:

    Article Title: Development and application of zebrafish liver 3D spheroids for assessing hepatotoxicity.
    Article Snippet: With the widespread adoption of the 3R principles, three-dimensional (3D) cell models are increasingly employed in toxicology.. However, research on 3D cell models in fish remains limited.. This study established a 3D zebrafish liver (ZFL) cell model and utilized it to assess the hepatotoxicity of N-(1,3-dimethylbutyl)-N′-phenyl-pphenylenediamine quinone (6PPD-Q) and N-Isopropyl-N'-phenyl-1,4-phenylenediamine quinone (IPPD-Q), respectively.

    Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke.
    Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair.

    Spheroids:

    Article Title: Development and application of zebrafish liver 3D spheroids for assessing hepatotoxicity.
    Article Snippet: With the widespread adoption of the 3R principles, three-dimensional (3D) cell models are increasingly employed in toxicology.. However, research on 3D cell models in fish remains limited.. This study established a 3D zebrafish liver (ZFL) cell model and utilized it to assess the hepatotoxicity of N-(1,3-dimethylbutyl)-N′-phenyl-pphenylenediamine quinone (6PPD-Q) and N-Isopropyl-N'-phenyl-1,4-phenylenediamine quinone (IPPD-Q), respectively.

    Inverted Microscopy:

    Article Title: Development and application of zebrafish liver 3D spheroids for assessing hepatotoxicity.
    Article Snippet: With the widespread adoption of the 3R principles, three-dimensional (3D) cell models are increasingly employed in toxicology.. However, research on 3D cell models in fish remains limited.. This study established a 3D zebrafish liver (ZFL) cell model and utilized it to assess the hepatotoxicity of N-(1,3-dimethylbutyl)-N′-phenyl-pphenylenediamine quinone (6PPD-Q) and N-Isopropyl-N'-phenyl-1,4-phenylenediamine quinone (IPPD-Q), respectively.

    Phase Contrast:

    Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke.
    Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair.

    Fluorescence:

    Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke.
    Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair.

    Article Title: Optimized gGBE enables robust G-to-Y conversion in mammalian cells and zebrafish embryos.
    Article Snippet: .. Fluorescence images were acquired using an inverted fluorescence imaging system (Ts2R, Nikon, Japan). .. GFP-positive cells were then sorted by flow cytometry (BD FACSAria III, USA).

    Article Title: Sex‐Specific Effects of Dexmedetomidine on Brain Microvascular Endothelial Cell Function After Traumatic Brain Injury in Juvenile Mice
    Article Snippet: Cells were then incubated with BioTracker 633 Red Mitochondria Dye (100 nM; MilliporeSigma, MA, USA) and BioTracker Mitochondrial Lipid Peroxide Live Cell Ferroptosis Indicator (100 nM; MilliporeSigma, MA, USA) for 30 min before imaging. .. Images were acquired using a Nikon Plan Apo 20×/0.9 objective mounted on a Nikon Eclipse TS2R fluorescence microscope (Nikon, NY, USA). ..

    Article Title: Prevotella histicola improves metabolic-associated fatty liver disease by promoting lipid droplet autophagy and inhibiting ER stress.
    Article Snippet: Metabolically associated fatty liver disease (MAFLD), a prevalent chronic liver disorder linked to metabolic dysfunction, is marked by hepatic lipid accumulation and inflammatory response.. Treatment options are limited, yet probiotics show potential in modulating the gut-liver axis.. Prevotella histicola (P. histicola), known for its anti-oxidant and anti-inflammatory features, provides protection against metabolic diseases, but its role in MAFLD remains unclear.

    Article Title: Integration of network toxicology and zebrafish models to elucidate bisphenol BP-induced cardiotoxicity.
    Article Snippet: Bisphenol BP (BPBP), a structural analog of bisphenol A (BPA), is increasingly utilized in industrial applications; however, its potential hazards to the cardiovascular system remain unexplored.. This study employed an integrated strategy combining network toxicology, molecular docking, and in vivo zebrafish models to systematically elucidate the cardiovascular toxicity of BPBP.. Network toxicology identified several candidate hub genes and pathways potentially associated with BPBP-induced Jo ur na l P re -p ro of Journal Pre-proof 2 cardiotoxicity.

    Article Title: Astragaloside IV improves MASLD via GCN5-LXRα/SREBP1c signaling pathway-mediated hepatic de novo lipogenesis
    Article Snippet: .. Intracellular lipid droplets were imaged for fluorescence using either a laser confocal microscope (Olympus FV3000) or an inverted fluorescence microscope (Nikon Ts2R). .. Intracellular neutral lipids were quantified using ImageJ (Version 1.5a).

    Microscopy:

    Article Title: Proteasome-dependent degradation of DAPK1 by wogonoside blocks PARthanatos and protects against ischemic stroke.
    Article Snippet: Ischemic stroke remains a leading cause of death and disability, yet effective pharmacological neuroprotection remains elusive.. ROS-induced PARthanatos—driven by PARP1 hyperactivation and the mitochondrial– apoptosis-inducing factor (AIF) death axis—is a principal executor of ischemic neuronal death.. However, direct PARP1 inhibition inevitably compromises essential DNA repair.

    Article Title: Sex‐Specific Effects of Dexmedetomidine on Brain Microvascular Endothelial Cell Function After Traumatic Brain Injury in Juvenile Mice
    Article Snippet: Cells were then incubated with BioTracker 633 Red Mitochondria Dye (100 nM; MilliporeSigma, MA, USA) and BioTracker Mitochondrial Lipid Peroxide Live Cell Ferroptosis Indicator (100 nM; MilliporeSigma, MA, USA) for 30 min before imaging. .. Images were acquired using a Nikon Plan Apo 20×/0.9 objective mounted on a Nikon Eclipse TS2R fluorescence microscope (Nikon, NY, USA). ..

    Article Title: Prevotella histicola improves metabolic-associated fatty liver disease by promoting lipid droplet autophagy and inhibiting ER stress.
    Article Snippet: Metabolically associated fatty liver disease (MAFLD), a prevalent chronic liver disorder linked to metabolic dysfunction, is marked by hepatic lipid accumulation and inflammatory response.. Treatment options are limited, yet probiotics show potential in modulating the gut-liver axis.. Prevotella histicola (P. histicola), known for its anti-oxidant and anti-inflammatory features, provides protection against metabolic diseases, but its role in MAFLD remains unclear.

    Article Title: CD73‐Overexpressing ADSCs Promote Neurogenic Bladder Repair via A2A–JAK1/STAT3‐Mediated Modulation of Inflammation and Macrophage Polarization
    Article Snippet: Tissue sections from all experimental groups were processed simultaneously with a commercial Masson staining kit (Solarbio, Beijing, China) to reduce interbatch variability. .. Representative images were captured using a Nikon Ts2R microscope (Nikon, Tokyo, Japan), and staining intensity was quantitatively analyzed with NIS‐Elements Viewer software. ..

    Article Title: Integration of network toxicology and zebrafish models to elucidate bisphenol BP-induced cardiotoxicity.
    Article Snippet: Bisphenol BP (BPBP), a structural analog of bisphenol A (BPA), is increasingly utilized in industrial applications; however, its potential hazards to the cardiovascular system remain unexplored.. This study employed an integrated strategy combining network toxicology, molecular docking, and in vivo zebrafish models to systematically elucidate the cardiovascular toxicity of BPBP.. Network toxicology identified several candidate hub genes and pathways potentially associated with BPBP-induced Jo ur na l P re -p ro of Journal Pre-proof 2 cardiotoxicity.

    Article Title: Astragaloside IV improves MASLD via GCN5-LXRα/SREBP1c signaling pathway-mediated hepatic de novo lipogenesis
    Article Snippet: .. Intracellular lipid droplets were imaged for fluorescence using either a laser confocal microscope (Olympus FV3000) or an inverted fluorescence microscope (Nikon Ts2R). .. Intracellular neutral lipids were quantified using ImageJ (Version 1.5a).

    Imaging:

    Article Title: Optimized gGBE enables robust G-to-Y conversion in mammalian cells and zebrafish embryos.
    Article Snippet: .. Fluorescence images were acquired using an inverted fluorescence imaging system (Ts2R, Nikon, Japan). .. GFP-positive cells were then sorted by flow cytometry (BD FACSAria III, USA).

    Article Title: Integration of network toxicology and zebrafish models to elucidate bisphenol BP-induced cardiotoxicity.
    Article Snippet: Bisphenol BP (BPBP), a structural analog of bisphenol A (BPA), is increasingly utilized in industrial applications; however, its potential hazards to the cardiovascular system remain unexplored.. This study employed an integrated strategy combining network toxicology, molecular docking, and in vivo zebrafish models to systematically elucidate the cardiovascular toxicity of BPBP.. Network toxicology identified several candidate hub genes and pathways potentially associated with BPBP-induced Jo ur na l P re -p ro of Journal Pre-proof 2 cardiotoxicity.

    Staining:

    Article Title: CD73‐Overexpressing ADSCs Promote Neurogenic Bladder Repair via A2A–JAK1/STAT3‐Mediated Modulation of Inflammation and Macrophage Polarization
    Article Snippet: Tissue sections from all experimental groups were processed simultaneously with a commercial Masson staining kit (Solarbio, Beijing, China) to reduce interbatch variability. .. Representative images were captured using a Nikon Ts2R microscope (Nikon, Tokyo, Japan), and staining intensity was quantitatively analyzed with NIS‐Elements Viewer software. ..

    Software:

    Article Title: CD73‐Overexpressing ADSCs Promote Neurogenic Bladder Repair via A2A–JAK1/STAT3‐Mediated Modulation of Inflammation and Macrophage Polarization
    Article Snippet: Tissue sections from all experimental groups were processed simultaneously with a commercial Masson staining kit (Solarbio, Beijing, China) to reduce interbatch variability. .. Representative images were captured using a Nikon Ts2R microscope (Nikon, Tokyo, Japan), and staining intensity was quantitatively analyzed with NIS‐Elements Viewer software. ..

    Confocal:

    Article Title: Astragaloside IV improves MASLD via GCN5-LXRα/SREBP1c signaling pathway-mediated hepatic de novo lipogenesis
    Article Snippet: .. Intracellular lipid droplets were imaged for fluorescence using either a laser confocal microscope (Olympus FV3000) or an inverted fluorescence microscope (Nikon Ts2R). .. Intracellular neutral lipids were quantified using ImageJ (Version 1.5a).



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    Image Search Results


    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Journal: Bioactive Materials

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1

    doi: 10.1016/j.bioactmat.2025.10.040

    Figure Lengend Snippet: Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Article Snippet: Following dewaxing and rehydration of femur sections, they were treated with TRAP staining solution (G105, Servicebio, Wuhan, China) at 37 °C for 30 min in the absence of light.

    Techniques: Immunohistochemical staining, Staining, Immunofluorescence