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Structured Review

Servicebio Inc trap kit
Overexpression of NLRP12 in PDLSCs alleviated the inflammatory responses and promoted periodontal regeneration in periodontitis rats. (A) The timeline demonstrated the experimental procedures. (B) The micro-CT scanning results. (C) Representative images of <t>H&E</t> <t>staining</t> (the black scale bars and white scale bars are 200 and 100 μm, respectively). (D) Representative images of Masson trichrome staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (E) Cementoenamel junction (CEJ)–alveolar bone crest (ABC) distance. (F) bone volume/total volume (BV/TV). (G) Loss of attachment in H&E staining. (H) Semiquantitative analysis of Masson trichrome staining. (I) Representative immunohistochemical images showing the expression level of inflammatory factor IL-6, IL-8, and IL-10, osteogenic factor COL1, and RUNX2, and <t>TRAP</t> staining showing the number of osteoclasts. Scale bar = 100 μm. (J) Quantitative analysis of IL-6-positive cells, IL-8-positive cells, IL-10-positive cells, COL1-positive cells, and RUNX2-positive cells. (K) Quantitative result of TRAP+ cells. Control group: without treatment. Periodontitis group: ligature-induced experimental periodontitis model. oeNC group: periodontitis group treated with oeNC PDLSCs. oeNLRP12 group: periodontitis group treated with oeNLRP12 PDLSCs. IHC: immunohistochemistry. IOD, integrated option density. The data are presented as mean ± SD ( n = 6 rats per group). *P < .1 , **P < .01 , ***P < .001 , ****P < .0001 .
Trap Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trap+kit/kit+staining+trap/pmc12905752-84-13-15
Average 86 stars, based on 1 article reviews
trap kit - by Bioz Stars, 2026-09
86/100 stars

Images

1) Product Images from "NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway"

Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway

Journal: International Dental Journal

doi: 10.1016/j.identj.2026.109417

Overexpression of NLRP12 in PDLSCs alleviated the inflammatory responses and promoted periodontal regeneration in periodontitis rats. (A) The timeline demonstrated the experimental procedures. (B) The micro-CT scanning results. (C) Representative images of H&E staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (D) Representative images of Masson trichrome staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (E) Cementoenamel junction (CEJ)–alveolar bone crest (ABC) distance. (F) bone volume/total volume (BV/TV). (G) Loss of attachment in H&E staining. (H) Semiquantitative analysis of Masson trichrome staining. (I) Representative immunohistochemical images showing the expression level of inflammatory factor IL-6, IL-8, and IL-10, osteogenic factor COL1, and RUNX2, and TRAP staining showing the number of osteoclasts. Scale bar = 100 μm. (J) Quantitative analysis of IL-6-positive cells, IL-8-positive cells, IL-10-positive cells, COL1-positive cells, and RUNX2-positive cells. (K) Quantitative result of TRAP+ cells. Control group: without treatment. Periodontitis group: ligature-induced experimental periodontitis model. oeNC group: periodontitis group treated with oeNC PDLSCs. oeNLRP12 group: periodontitis group treated with oeNLRP12 PDLSCs. IHC: immunohistochemistry. IOD, integrated option density. The data are presented as mean ± SD ( n = 6 rats per group). *P < .1 , **P < .01 , ***P < .001 , ****P < .0001 .
Figure Legend Snippet: Overexpression of NLRP12 in PDLSCs alleviated the inflammatory responses and promoted periodontal regeneration in periodontitis rats. (A) The timeline demonstrated the experimental procedures. (B) The micro-CT scanning results. (C) Representative images of H&E staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (D) Representative images of Masson trichrome staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (E) Cementoenamel junction (CEJ)–alveolar bone crest (ABC) distance. (F) bone volume/total volume (BV/TV). (G) Loss of attachment in H&E staining. (H) Semiquantitative analysis of Masson trichrome staining. (I) Representative immunohistochemical images showing the expression level of inflammatory factor IL-6, IL-8, and IL-10, osteogenic factor COL1, and RUNX2, and TRAP staining showing the number of osteoclasts. Scale bar = 100 μm. (J) Quantitative analysis of IL-6-positive cells, IL-8-positive cells, IL-10-positive cells, COL1-positive cells, and RUNX2-positive cells. (K) Quantitative result of TRAP+ cells. Control group: without treatment. Periodontitis group: ligature-induced experimental periodontitis model. oeNC group: periodontitis group treated with oeNC PDLSCs. oeNLRP12 group: periodontitis group treated with oeNLRP12 PDLSCs. IHC: immunohistochemistry. IOD, integrated option density. The data are presented as mean ± SD ( n = 6 rats per group). *P < .1 , **P < .01 , ***P < .001 , ****P < .0001 .

Techniques Used: Over Expression, Micro-CT, Staining, Immunohistochemical staining, Expressing, Control, Immunohistochemistry

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Article Snippet: .. TRAP staining of osteoclasts in decalcified bone sections was performed by Wuhan Servicebio Technology Co., Ltd. (Wuhan, Hubei, China) following a standard automated histology protocol. ..

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Article Snippet: .. Sections were stained with hematoxylin and eosin (H&E) staining kit (#G1005, Servicebio, China). ..

Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
Article Snippet: .. After paraffin embedding and sectioning (5 μm), sections were stained with H&E (#G1005, Servicebio, China) or for tartrate-resistant acid phosphatase (TRAP) activity using a TRAP staining kit (#G1050, Servicebio, China). .. For immunofluorescence AR TIC LE IN PR ES S staining, sections were incubated with a rabbit monoclonal anti-COL1 antibody (1:200, #ab270993, Abcam) overnight at 4°C, followed by incubation with a goat anti-rabbit Alexa Fluor 555 secondary antibody (1:500, #A21428, Invitrogen) for 30 minutes at 37°C.

Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
Article Snippet: Nuclei were counterstained with DAPI (#D8200, Solarbio) for 10 minutes. .. Cells: Cells were fixed with 4% paraformaldehyde for 10 minutes and stained for TRAP activity (#G1050, Servicebio, China) according to the manufacturer's protocol. .. All stained sections and cells were imaged using a confocal laser scanning microscope (Olympus, Japan) or a light microscope.

Article Title: Amuc_1473 Links Gut Microbes to Skeletal Homeostasis and Counteracts Multifactorial Osteoporosis.
Article Snippet: .. Paraffin-embedded sections ere cut into 5- μm-thick slices for TRAP staining using a kit from ervicebio, immunohistochemical staining for OCN with antiody from Servicebio, or immunofluorescence staining for MUC2 sing antibody from Proteintech. .. Images were captured using n Olympus CX31 optical microscope (Olympus, Tokyo, Japan) r Zeiss ApoTome microscope (ZEISS, Oberkochen, Germany).

Article Title: A New Wnt1 Mutant Rat Model of Osteogenesis Imperfecta and Its Application in AAV9‐Mediated Gene Therapy
Article Snippet: .. Sections were stained with hematoxylin and eosin (H&E), Masson′s trichrome, and a tartrate‐resistant Acid phosphatase (TRAP) commercial staining kit (TRAP Staining Kit, Servicebio, China). .. Stained sections were visualized with an optical microscope (NIKON ECLIPSE E100, Nikon, Japan).

Article Title: Phenylethanoid Glycosides from Cistanche tubulosa (Schenk) Wight Improve Radiation-Induced Bone Injury and Reduce Grem1 Expression in Osteoblasts.
Article Snippet: .. Freshly prepared TRAP incubation solution (Servicebio, G1050, Wuhan, China) was added, and sections were incubated at 37 ◦C for 20 min. After staining, nuclei were counterstained with hematoxylin for approximately 15 s, differentiated, and blued (sections were immersed into the reverse blue solution for 10 s, then removed, and washed with water). ..

Activity Assay:

Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
Article Snippet: .. After paraffin embedding and sectioning (5 μm), sections were stained with H&E (#G1005, Servicebio, China) or for tartrate-resistant acid phosphatase (TRAP) activity using a TRAP staining kit (#G1050, Servicebio, China). .. For immunofluorescence AR TIC LE IN PR ES S staining, sections were incubated with a rabbit monoclonal anti-COL1 antibody (1:200, #ab270993, Abcam) overnight at 4°C, followed by incubation with a goat anti-rabbit Alexa Fluor 555 secondary antibody (1:500, #A21428, Invitrogen) for 30 minutes at 37°C.

Article Title: SMN deficiency contributes to osteoporosis in spinal muscular atrophy by impairing Snap23 meditated muscle-derived extracellular vesicle secretion.
Article Snippet: Nuclei were counterstained with DAPI (#D8200, Solarbio) for 10 minutes. .. Cells: Cells were fixed with 4% paraformaldehyde for 10 minutes and stained for TRAP activity (#G1050, Servicebio, China) according to the manufacturer's protocol. .. All stained sections and cells were imaged using a confocal laser scanning microscope (Olympus, Japan) or a light microscope.

Immunohistochemical staining:

Article Title: Amuc_1473 Links Gut Microbes to Skeletal Homeostasis and Counteracts Multifactorial Osteoporosis.
Article Snippet: .. Paraffin-embedded sections ere cut into 5- μm-thick slices for TRAP staining using a kit from ervicebio, immunohistochemical staining for OCN with antiody from Servicebio, or immunofluorescence staining for MUC2 sing antibody from Proteintech. .. Images were captured using n Olympus CX31 optical microscope (Olympus, Tokyo, Japan) r Zeiss ApoTome microscope (ZEISS, Oberkochen, Germany).

Immunofluorescence:

Article Title: Amuc_1473 Links Gut Microbes to Skeletal Homeostasis and Counteracts Multifactorial Osteoporosis.
Article Snippet: .. Paraffin-embedded sections ere cut into 5- μm-thick slices for TRAP staining using a kit from ervicebio, immunohistochemical staining for OCN with antiody from Servicebio, or immunofluorescence staining for MUC2 sing antibody from Proteintech. .. Images were captured using n Olympus CX31 optical microscope (Olympus, Tokyo, Japan) r Zeiss ApoTome microscope (ZEISS, Oberkochen, Germany).

Incubation:

Article Title: Phenylethanoid Glycosides from Cistanche tubulosa (Schenk) Wight Improve Radiation-Induced Bone Injury and Reduce Grem1 Expression in Osteoblasts.
Article Snippet: .. Freshly prepared TRAP incubation solution (Servicebio, G1050, Wuhan, China) was added, and sections were incubated at 37 ◦C for 20 min. After staining, nuclei were counterstained with hematoxylin for approximately 15 s, differentiated, and blued (sections were immersed into the reverse blue solution for 10 s, then removed, and washed with water). ..



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Image Search Results


Overexpression of NLRP12 in PDLSCs alleviated the inflammatory responses and promoted periodontal regeneration in periodontitis rats. (A) The timeline demonstrated the experimental procedures. (B) The micro-CT scanning results. (C) Representative images of H&E staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (D) Representative images of Masson trichrome staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (E) Cementoenamel junction (CEJ)–alveolar bone crest (ABC) distance. (F) bone volume/total volume (BV/TV). (G) Loss of attachment in H&E staining. (H) Semiquantitative analysis of Masson trichrome staining. (I) Representative immunohistochemical images showing the expression level of inflammatory factor IL-6, IL-8, and IL-10, osteogenic factor COL1, and RUNX2, and TRAP staining showing the number of osteoclasts. Scale bar = 100 μm. (J) Quantitative analysis of IL-6-positive cells, IL-8-positive cells, IL-10-positive cells, COL1-positive cells, and RUNX2-positive cells. (K) Quantitative result of TRAP+ cells. Control group: without treatment. Periodontitis group: ligature-induced experimental periodontitis model. oeNC group: periodontitis group treated with oeNC PDLSCs. oeNLRP12 group: periodontitis group treated with oeNLRP12 PDLSCs. IHC: immunohistochemistry. IOD, integrated option density. The data are presented as mean ± SD ( n = 6 rats per group). *P < .1 , **P < .01 , ***P < .001 , ****P < .0001 .

Journal: International Dental Journal

Article Title: NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling Pathway

doi: 10.1016/j.identj.2026.109417

Figure Lengend Snippet: Overexpression of NLRP12 in PDLSCs alleviated the inflammatory responses and promoted periodontal regeneration in periodontitis rats. (A) The timeline demonstrated the experimental procedures. (B) The micro-CT scanning results. (C) Representative images of H&E staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (D) Representative images of Masson trichrome staining (the black scale bars and white scale bars are 200 and 100 μm, respectively). (E) Cementoenamel junction (CEJ)–alveolar bone crest (ABC) distance. (F) bone volume/total volume (BV/TV). (G) Loss of attachment in H&E staining. (H) Semiquantitative analysis of Masson trichrome staining. (I) Representative immunohistochemical images showing the expression level of inflammatory factor IL-6, IL-8, and IL-10, osteogenic factor COL1, and RUNX2, and TRAP staining showing the number of osteoclasts. Scale bar = 100 μm. (J) Quantitative analysis of IL-6-positive cells, IL-8-positive cells, IL-10-positive cells, COL1-positive cells, and RUNX2-positive cells. (K) Quantitative result of TRAP+ cells. Control group: without treatment. Periodontitis group: ligature-induced experimental periodontitis model. oeNC group: periodontitis group treated with oeNC PDLSCs. oeNLRP12 group: periodontitis group treated with oeNLRP12 PDLSCs. IHC: immunohistochemistry. IOD, integrated option density. The data are presented as mean ± SD ( n = 6 rats per group). *P < .1 , **P < .01 , ***P < .001 , ****P < .0001 .

Article Snippet: To detect multinucleated osteoclasts, tartrate-resistant acid phosphatase (TRAP) staining was performed with a TRAP kit (Servicebio).

Techniques: Over Expression, Micro-CT, Staining, Immunohistochemical staining, Expressing, Control, Immunohistochemistry