lineage cell depletion kit (Miltenyi Biotec)

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Miltenyi Biotec lineage cell depletion kit
$Bone marrow progenitor cells express substance P and calcitonin gene-related peptide receptors. A, Immunostaining of <t>lineage-negative</t> (Lin−) cells (selected with the use of magnetic beads and a cocktail of antibodies against committed hematopoietic cells) expressing neurokinin 1 (NK1) (a), receptor activity-modifying protein 1 (RAMP-1) (b), and calcitonin receptor-like receptor (CRLR) (c) (green). Nuclei are stained with 4′, 6-diamidino-2-phenylindole (blue). Immunoreactivity was not detected when primary antibodies were omitted (negative control, d). B through D, Flow cytometry confirms the expression of neuropeptide receptors in progenitor cells. Typical scatterplots and bar graphs show the analyzed data. A substantial fraction of NK1+, RAMP-1+, and CRLR+ cells are Lin− and express the progenitor <t>cell</t> markers <t>c-Kit</t> (c-Kit+) and Sca-1 (Sca-1+).$
Lineage Cell Depletion Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 694 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transfer+function+of+a+linearized+model/Lineage+Cell+Depletion+Kit%2C+mouse/pmc03616366-178-23-35
Average 96 stars, based on 694 article reviews

lineage cell depletion kit - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Role for Substance P-Based Nociceptive Signaling in Progenitor Cell Activation and Angiogenesis During Ischemia in Mice and in Human Subjects"

Article Title: Role for Substance P-Based Nociceptive Signaling in Progenitor Cell Activation and Angiogenesis During Ischemia in Mice and in Human Subjects

Journal: Circulation

doi: 10.1161/CIRCULATIONAHA.111.089763

$Bone marrow progenitor cells express substance P and calcitonin gene-related peptide receptors. A, Immunostaining of lineage-negative (Lin−) cells (selected with the use of magnetic beads and a cocktail of antibodies against committed hematopoietic cells) expressing neurokinin 1 (NK1) (a), receptor activity-modifying protein 1 (RAMP-1) (b), and calcitonin receptor-like receptor (CRLR) (c) (green). Nuclei are stained with 4′, 6-diamidino-2-phenylindole (blue). Immunoreactivity was not detected when primary antibodies were omitted (negative control, d). B through D, Flow cytometry confirms the expression of neuropeptide receptors in progenitor cells. Typical scatterplots and bar graphs show the analyzed data. A substantial fraction of NK1+, RAMP-1+, and CRLR+ cells are Lin− and express the progenitor cell markers c-Kit (c-Kit+) and Sca-1 (Sca-1+).$
Figure Legend Snippet: Bone marrow progenitor cells express substance P and calcitonin gene-related peptide receptors. A, Immunostaining of lineage-negative (Lin−) cells (selected with the use of magnetic beads and a cocktail of antibodies against committed hematopoietic cells) expressing neurokinin 1 (NK1) (a), receptor activity-modifying protein 1 (RAMP-1) (b), and calcitonin receptor-like receptor (CRLR) (c) (green). Nuclei are stained with 4′, 6-diamidino-2-phenylindole (blue). Immunoreactivity was not detected when primary antibodies were omitted (negative control, d). B through D, Flow cytometry confirms the expression of neuropeptide receptors in progenitor cells. Typical scatterplots and bar graphs show the analyzed data. A substantial fraction of NK1+, RAMP-1+, and CRLR+ cells are Lin− and express the progenitor cell markers c-Kit (c-Kit+) and Sca-1 (Sca-1+).

Techniques Used: Immunostaining, Magnetic Beads, Expressing, Activity Assay, Staining, Negative Control, Flow Cytometry

$Substance P (SP) and calcitonin gene-related peptide (CGRP) exert chemoattractant effects on mouse bone marrow cells. A, SP and CGRP induce migration of lineage-negative (Lin−) bone marrow (BM) cells. Data are expressed as fold increase of vehicle (veh). B, SP (100 nmol/L for 15 minutes) induces phosphorylation/activation of Akt in Lin− cells, which is prevented by the phosphoinositide-3 kinase antagonist LY 294002 (LY) (15 μmol/L). *P<0.05 vs control; n=6 to 9. LY 294002 pretreatment also prevents SP-induced progenitor cell migration. *P<0.05 vs control; #P<0.05 vs SP only; n=3. C and D, Primary culture of mouse dorsal root ganglia (DRG) and respective conditioned medium (CM) induces cell migration (C) leading to an enrichment of Lin− c-Kit+Sca-1+ PC in the migrated fraction (D). E, The migratory effect induced by DRG is reduced by antagonists for CGRP (CGRP8–37, 1000 nmol/L) and NK1 (RP67580, 100 nmol/L). *P<0.05 vs vehicle; n=6; #P<0.05 vs DRG alone; n=5.$
Figure Legend Snippet: Substance P (SP) and calcitonin gene-related peptide (CGRP) exert chemoattractant effects on mouse bone marrow cells. A, SP and CGRP induce migration of lineage-negative (Lin−) bone marrow (BM) cells. Data are expressed as fold increase of vehicle (veh). B, SP (100 nmol/L for 15 minutes) induces phosphorylation/activation of Akt in Lin− cells, which is prevented by the phosphoinositide-3 kinase antagonist LY 294002 (LY) (15 μmol/L). *P<0.05 vs control; n=6 to 9. LY 294002 pretreatment also prevents SP-induced progenitor cell migration. *P<0.05 vs control; #P<0.05 vs SP only; n=3. C and D, Primary culture of mouse dorsal root ganglia (DRG) and respective conditioned medium (CM) induces cell migration (C) leading to an enrichment of Lin− c-Kit+Sca-1+ PC in the migrated fraction (D). E, The migratory effect induced by DRG is reduced by antagonists for CGRP (CGRP8–37, 1000 nmol/L) and NK1 (RP67580, 100 nmol/L). *P<0.05 vs vehicle; n=6; #P<0.05 vs DRG alone; n=5.

Techniques Used: Migration, Phospho-proteomics, Activation Assay, Control

Isolation:

Article Title: Optimising Haematopoietic Stem Cell Transplantation: Enhancing Myeloablation Sensitivity and Alleviating Anaemia Using Roxadustat ( FG ‐4592)
Article Snippet: .. Lineage‐positive (Lin + ) and lineage‐negative (Lin − ) BM‐MNCs were isolated using a magnetic cell sorting system (MACS; Miltenyi Biotec, 130‐090‐858): (1) Haematopoietic stem/progenitor cells were evaluated using Sca‐1 (BD Biosciences 557405) and c‐Kit (BD Biosciences 553356) antibodies. (2) Erythroid cells were labelled using Ter119 (Invitrogen 12‐5921‐82). .. HIF‐1α expression was detected by flow cytometry using PE‐conjugated anti–HIF‐1α antibody generated with a Lightning‐Link conjugation kit (Abcam ab102918; antibody NB100‐134, Novus Biologicals).

Article Title: CRISPR/Cas9 gene editing in hematopoietic stem and progenitor cells to accelerate the translation of cellular therapies and immunotherapies
Article Snippet: .. Isolation and culture of Lin– murine HSPCs Mouse hematopoietic stem and progenitor cells were obtained from the bone marrow of tibias and femurs of 5–9-week-old C57BL/6J mice after red blood cell lysis and depletion of lineage-positive populations using a magnetic bead isolation kit (Miltenyi Biotec, cat. 130-090-858), as previously described (28). .. Cells were plated at 0.5 × 106-1 × 106 cells/ml in SFEMII+10x media (SFEMII: StemCell Technologies, cat. 9655; CD34+ Expansion Supplement (10X): StemCell Technologies, cat. 02691) in 6-well or 24-well plates.

FACS:

Article Title: Therapeutic activity of a hematopoietic stem cell-delivered cell-penetrating frataxin in Friedreich’s ataxia models
Article Snippet: Femurs and tibias were dissected from male donor mice (either FRDA or C57BL/6) and bone marrow cells obtained by flushing with RPMI (Gibco) media. .. Cells were pelleted and enriched for Ter119 - , Gr1, Mac1 - , B220 - , CD4 − , CD8 − , IL7R − and Sca + , cKit + cells by magnetic cell sorting using mouse Lineage Cell Depletion Kit (Miltenyi Biotech) according to the manufacturer’s protocol. ..

Magnetic Cell Separation:

Article Title: Spatially resolved, multimodal in vivo Perturb-seq using antibody-based cell hashing
Article Snippet: .. Lineage negative, Ckit+ cells were enriched using MACS beads (Miltenyi #130-090-858, #130-091-224). .. HPCs were then expanded for 10 days in HemEx-Type9AØ (Iwai #A5P10P01C) supplemented with 10 ng/mL SCF (PeproTech) and 100 ng/mL Tpo (PeProTech).

Red Blood Cell Lysis:

Purification:

Article Title: Selection of human hematopoietic stem cells bearing the intended functional edit by transient AND-gate reporters.
Article Snippet: .. HSPCs were purified by Lin− selection using the mouse lineage cell depletion kit (Miltenyi Biotec) according to the manufacturer’s instructions. .. Cells were then cultured in serum-free StemSpan medium (StemCell Technologies) containing penicillin, streptomycin, glutamine and a combination of mouse cytokines (20 ng ml−1 IL-3, 100 ng ml−1 SCF, 100 ng ml−1 Flt-3L, 50 ng ml−1 TPO; PeproTech), at a concentration of 106 cells per ml.

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lineage cell depletion kit (Miltenyi Biotec)

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1) Product Images from "Role for Substance P-Based Nociceptive Signaling in Progenitor Cell Activation and Angiogenesis During Ischemia in Mice and in Human Subjects"

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Isolation:

FACS:

Magnetic Cell Separation:

Red Blood Cell Lysis:

Purification:

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