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transfected construct  (Addgene inc)


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    Structured Review

    Addgene inc transfected construct
    Transfected Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transfected+construct/pAAV-hSyn-hChR2(H134R)-EYFP+(Plasmid+%2326973)/pmc12074639-0-0-9
    Average 96 stars, based on 243 article reviews
    transfected construct - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Virus:

    Article Title: A developmental switch in corticoclaustral signaling.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Aves Labs Cat#: gfp1010; RRID: AB_2307313 Rabbit anti-Nr2f2 Abcam Cat#: ab211776; RRID: AB_2893028 Goat anti-Nurr1 R&D Systems Cat#: AF2156; RRID: AB_2153894 Goat anti-PV Swant Cat#: PVG213; RRID: AB_2313848 Rat anti-SST Millipore Cat#: MAB354; RRID: AB_2255365 Mouse anti-Tle4 Santa Cruz Biotechnology Cat#: sc365406; RRID: AB_10841582 Chicken anti-rat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21472, RRID: AB_2535875 Donkey anti-Chicken IgY Alexa Fluor 488 Thermo Fisher Scientific Cat#: A-78948; RRID: AB_2921070 Donkey anti-Chicken IgY Alexa Fluor 647 Thermo Fisher Scientific Cat#: A-78952; RRID: AB_2921074 Donkey anti-Goat IgG Alexa Fluor 555 Thermo Fisher Scientific Cat# A-21432, RRID: AB_2535853 Donkey anti-Goat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447, RRID: AB_2535864 Donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21202, RRID: AB_141607 Bacterial and virus strains pAAV-CAG-GFP Gift from Edward Boyden RRID: Addgene_37825-AAVrg pAAV-CAG-tdTomato Gift from Edward Boyden RRID: Addgene_59462-AAVrg pAAV-hSyn-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26973-AAV1 pAAV-CaMKIIa-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26969-AAV1 pAAV-hSyn-Cre-P2A-dTomato Gift from Rylan Larsen RRID: Addgene_107738-AAVrg pAAV-hSyn-DIO-hM4D(Gi)-mCherry Gift from Bryan Roth RRID: Addgene_44362-AAV5 Chemicals, peptides, and recombinant proteins Clozapine-n-oxide Enzo Life Sciences Cat#: BML-NS105 DiD stain Thermo Fisher Scientific Cat#: D7757 DiI stain Thermo Fisher Scientific Cat#: D3911 Experimental models: organisms/strains Mouse: Ai14 Jackson Laboratories RRID: IMSR_JAX: 007914 Mouse: C57BL/6J Jackson Laboratories RRID: IMSR_JAX: 000664 Mouse: PV-cre Jackson Laboratories RRID: IMSR_JAX:017320 Software and algorithms Illustrator Adobe RRID: SCR_010279 ImageJ (Fiji) National Institute of Health RRID: SCR_003070 MATLAB MathWorks RRID: SCR_001622 Prism Graphpad RRID: SCR_02798 Other Fiber optic cannula RWD Cat#: R-FOC-L200C-39NA Recording electrode (4 channels) Neuronexus Cat#: Q1x4-10mm-50-177-Q4 ll OPEN ACCESS Current Biology 36, 1–10.e1–e5, April 6, 2026 e1 Report .. Stereotaxic injection of viral vectors Adeno-associated viruses (AAVs) were purchased from Addgene (MA, USA) at titer ≥ 1×1012 vg/ml. pAAV-CaMKIIa-hChR2(H134R)EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26969; http://n2t.net/addgene:26969; RRID:Addgene_26969).78 pAAVCAG-GFP was a gift from Edward Boyden (Addgene viral prep # 37825-AAVrg.T; RRID:Addgene_37825). pAAV-CAG-tdTomato (codon diversified) was a gift from Edward Boyden (viral prep # 59462-AAVrg; RRID:Addgene_59462). pAAV-hSyn-DIO-hM4D(Gi)mCherry was a gift from Bryan Roth (Addgene viral prep # 44362-AAV5); RRID:Addgene_44362).79 pAAV-hSyn-Cre-P2A-dTomato was a gift from Rylan Larsen (Addgene viral prep # 107738-AAVrg; RRID:Addgene_107738).

    Recombinant:

    Article Title: A developmental switch in corticoclaustral signaling.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Aves Labs Cat#: gfp1010; RRID: AB_2307313 Rabbit anti-Nr2f2 Abcam Cat#: ab211776; RRID: AB_2893028 Goat anti-Nurr1 R&D Systems Cat#: AF2156; RRID: AB_2153894 Goat anti-PV Swant Cat#: PVG213; RRID: AB_2313848 Rat anti-SST Millipore Cat#: MAB354; RRID: AB_2255365 Mouse anti-Tle4 Santa Cruz Biotechnology Cat#: sc365406; RRID: AB_10841582 Chicken anti-rat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21472, RRID: AB_2535875 Donkey anti-Chicken IgY Alexa Fluor 488 Thermo Fisher Scientific Cat#: A-78948; RRID: AB_2921070 Donkey anti-Chicken IgY Alexa Fluor 647 Thermo Fisher Scientific Cat#: A-78952; RRID: AB_2921074 Donkey anti-Goat IgG Alexa Fluor 555 Thermo Fisher Scientific Cat# A-21432, RRID: AB_2535853 Donkey anti-Goat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447, RRID: AB_2535864 Donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21202, RRID: AB_141607 Bacterial and virus strains pAAV-CAG-GFP Gift from Edward Boyden RRID: Addgene_37825-AAVrg pAAV-CAG-tdTomato Gift from Edward Boyden RRID: Addgene_59462-AAVrg pAAV-hSyn-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26973-AAV1 pAAV-CaMKIIa-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26969-AAV1 pAAV-hSyn-Cre-P2A-dTomato Gift from Rylan Larsen RRID: Addgene_107738-AAVrg pAAV-hSyn-DIO-hM4D(Gi)-mCherry Gift from Bryan Roth RRID: Addgene_44362-AAV5 Chemicals, peptides, and recombinant proteins Clozapine-n-oxide Enzo Life Sciences Cat#: BML-NS105 DiD stain Thermo Fisher Scientific Cat#: D7757 DiI stain Thermo Fisher Scientific Cat#: D3911 Experimental models: organisms/strains Mouse: Ai14 Jackson Laboratories RRID: IMSR_JAX: 007914 Mouse: C57BL/6J Jackson Laboratories RRID: IMSR_JAX: 000664 Mouse: PV-cre Jackson Laboratories RRID: IMSR_JAX:017320 Software and algorithms Illustrator Adobe RRID: SCR_010279 ImageJ (Fiji) National Institute of Health RRID: SCR_003070 MATLAB MathWorks RRID: SCR_001622 Prism Graphpad RRID: SCR_02798 Other Fiber optic cannula RWD Cat#: R-FOC-L200C-39NA Recording electrode (4 channels) Neuronexus Cat#: Q1x4-10mm-50-177-Q4 ll OPEN ACCESS Current Biology 36, 1–10.e1–e5, April 6, 2026 e1 Report .. Stereotaxic injection of viral vectors Adeno-associated viruses (AAVs) were purchased from Addgene (MA, USA) at titer ≥ 1×1012 vg/ml. pAAV-CaMKIIa-hChR2(H134R)EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26969; http://n2t.net/addgene:26969; RRID:Addgene_26969).78 pAAVCAG-GFP was a gift from Edward Boyden (Addgene viral prep # 37825-AAVrg.T; RRID:Addgene_37825). pAAV-CAG-tdTomato (codon diversified) was a gift from Edward Boyden (viral prep # 59462-AAVrg; RRID:Addgene_59462). pAAV-hSyn-DIO-hM4D(Gi)mCherry was a gift from Bryan Roth (Addgene viral prep # 44362-AAV5); RRID:Addgene_44362).79 pAAV-hSyn-Cre-P2A-dTomato was a gift from Rylan Larsen (Addgene viral prep # 107738-AAVrg; RRID:Addgene_107738).

    Staining:

    Article Title: A developmental switch in corticoclaustral signaling.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Aves Labs Cat#: gfp1010; RRID: AB_2307313 Rabbit anti-Nr2f2 Abcam Cat#: ab211776; RRID: AB_2893028 Goat anti-Nurr1 R&D Systems Cat#: AF2156; RRID: AB_2153894 Goat anti-PV Swant Cat#: PVG213; RRID: AB_2313848 Rat anti-SST Millipore Cat#: MAB354; RRID: AB_2255365 Mouse anti-Tle4 Santa Cruz Biotechnology Cat#: sc365406; RRID: AB_10841582 Chicken anti-rat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21472, RRID: AB_2535875 Donkey anti-Chicken IgY Alexa Fluor 488 Thermo Fisher Scientific Cat#: A-78948; RRID: AB_2921070 Donkey anti-Chicken IgY Alexa Fluor 647 Thermo Fisher Scientific Cat#: A-78952; RRID: AB_2921074 Donkey anti-Goat IgG Alexa Fluor 555 Thermo Fisher Scientific Cat# A-21432, RRID: AB_2535853 Donkey anti-Goat IgG Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447, RRID: AB_2535864 Donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21202, RRID: AB_141607 Bacterial and virus strains pAAV-CAG-GFP Gift from Edward Boyden RRID: Addgene_37825-AAVrg pAAV-CAG-tdTomato Gift from Edward Boyden RRID: Addgene_59462-AAVrg pAAV-hSyn-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26973-AAV1 pAAV-CaMKIIa-hChR2(H134R)-EYFP Gift from Karl Deisseroth RRID: Addgene_26969-AAV1 pAAV-hSyn-Cre-P2A-dTomato Gift from Rylan Larsen RRID: Addgene_107738-AAVrg pAAV-hSyn-DIO-hM4D(Gi)-mCherry Gift from Bryan Roth RRID: Addgene_44362-AAV5 Chemicals, peptides, and recombinant proteins Clozapine-n-oxide Enzo Life Sciences Cat#: BML-NS105 DiD stain Thermo Fisher Scientific Cat#: D7757 DiI stain Thermo Fisher Scientific Cat#: D3911 Experimental models: organisms/strains Mouse: Ai14 Jackson Laboratories RRID: IMSR_JAX: 007914 Mouse: C57BL/6J Jackson Laboratories RRID: IMSR_JAX: 000664 Mouse: PV-cre Jackson Laboratories RRID: IMSR_JAX:017320 Software and algorithms Illustrator Adobe RRID: SCR_010279 ImageJ (Fiji) National Institute of Health RRID: SCR_003070 MATLAB MathWorks RRID: SCR_001622 Prism Graphpad RRID: SCR_02798 Other Fiber optic cannula RWD Cat#: R-FOC-L200C-39NA Recording electrode (4 channels) Neuronexus Cat#: Q1x4-10mm-50-177-Q4 ll OPEN ACCESS Current Biology 36, 1–10.e1–e5, April 6, 2026 e1 Report .. Stereotaxic injection of viral vectors Adeno-associated viruses (AAVs) were purchased from Addgene (MA, USA) at titer ≥ 1×1012 vg/ml. pAAV-CaMKIIa-hChR2(H134R)EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26969; http://n2t.net/addgene:26969; RRID:Addgene_26969).78 pAAVCAG-GFP was a gift from Edward Boyden (Addgene viral prep # 37825-AAVrg.T; RRID:Addgene_37825). pAAV-CAG-tdTomato (codon diversified) was a gift from Edward Boyden (viral prep # 59462-AAVrg; RRID:Addgene_59462). pAAV-hSyn-DIO-hM4D(Gi)mCherry was a gift from Bryan Roth (Addgene viral prep # 44362-AAV5); RRID:Addgene_44362).79 pAAV-hSyn-Cre-P2A-dTomato was a gift from Rylan Larsen (Addgene viral prep # 107738-AAVrg; RRID:Addgene_107738).

    Injection:

    Article Title: Differential cortical and thalamic engagement of cholinergic interneurons in the nucleus accumbens core
    Article Snippet: .. For anterograde anatomy and physiology experiments examining inputs onto CINs, AAV1-hSyn-hChR2(H134R)-EYFP (360 nL) (Addgene, 26973-AAV1) or AAV1-CaMKII-hChR2(H134R)-mCherry (360 nL) (Addgene, 26975-AAV1) was injected into mPFC or mTH of ChAT-eGFP. ..



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    Image Search Results


    ( A - D ) Pannexins were expressed in HEK293 cells and voltage clamp recordings were performed at –60 mV. Both the first round ( A and B ) and the second round ( C and D ) fractions are shown. Blue bars indicate application of each fraction. ( E ) Current densities from second-round fractions. The number of recordings from different cells for each experiment (biological replicates) is shown in parenthesis. Error bars represent SEM. ( F ) and ( G ) Whole-cell currents obtained with vector-transfected control cells. Application of fraction #12 from the first round and fraction #7 from the second round are shown. Figure 1—figure supplement 2—source data 1. Electrophysiology data used for .

    Journal: eLife

    Article Title: ATP-release pannexin channels are gated by lysophospholipids

    doi: 10.7554/eLife.107067

    Figure Lengend Snippet: ( A - D ) Pannexins were expressed in HEK293 cells and voltage clamp recordings were performed at –60 mV. Both the first round ( A and B ) and the second round ( C and D ) fractions are shown. Blue bars indicate application of each fraction. ( E ) Current densities from second-round fractions. The number of recordings from different cells for each experiment (biological replicates) is shown in parenthesis. Error bars represent SEM. ( F ) and ( G ) Whole-cell currents obtained with vector-transfected control cells. Application of fraction #12 from the first round and fraction #7 from the second round are shown. Figure 1—figure supplement 2—source data 1. Electrophysiology data used for .

    Article Snippet: Transfected construct ( Homo-sapiens ) , PANX1 - shRNA #2 , BROAD Institute Genetic Perturbation Platform , TRCN0000154636 , Lentiviral construct to transfect and express the shRNA.

    Techniques: Plasmid Preparation, Transfection, Control

    Journal: eLife

    Article Title: ATP-release pannexin channels are gated by lysophospholipids

    doi: 10.7554/eLife.107067

    Figure Lengend Snippet:

    Article Snippet: Transfected construct ( Homo-sapiens ) , PANX1 - shRNA #2 , BROAD Institute Genetic Perturbation Platform , TRCN0000154636 , Lentiviral construct to transfect and express the shRNA.

    Techniques: Recombinant, Synthesized, Expressing, Plasmid Preparation, Transfection, Construct, shRNA

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    Article Title: N6-methyladenosine in DNA promotes genome stability

    doi: 10.7554/eLife.101626

    Figure Lengend Snippet:

    Article Snippet: Transfected construct ( Homo sapiens ) , Human: DLD-1 UNG KO Clone E7 + pCMV_Cry2_mCherry_UNG2_ DPIP , This paper , This paper , Pfizer, Inc.

    Techniques: Transfection, Construct, Recombinant, Retroviral, Plasmid Preparation, Sequencing, Purification, MTS Assay, Saline, Cell Culture, Labeling, Mass Spectrometry, Blocking Assay, Electroporation, CRISPR, Gene Expression, TaqMan Assay, Multiplex sample analysis, Protease Inhibitor, Software, Genome Wide, Knock-Out, Immunofluorescence, Microscopy, Imaging, Western Blot