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transcriptor reverse transcriptase enzyme  (Millipore)


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    Structured Review

    Millipore transcriptor reverse transcriptase enzyme
    Transcriptor Reverse Transcriptase Enzyme, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptor+reverse+transcriptase+enzyme/reverse+transcriptase/pm39118304-87-6-10
    Average 90 stars, based on 1 article reviews
    transcriptor reverse transcriptase enzyme - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Expansion of Cord Blood CD34 + Cells in Presence of zVADfmk and zLLYfmk Improved Their In Vitro Functionality and In Vivo Engraftment in NOD/SCID Mouse
    Article Snippet: The eluted mRNA were reverse transcribed to cDNA by using reverse transcriptase (Sigma Aldrich, St Loius, MO) and oligo-dT primers (invitrogen) as per the instructions and PCR was performed with specific primers.

    Article Title: Maternal High-Fat Diet Multigenerationally Programs HPA Function and Behaviors in Male Rat Offspring.
    Article Snippet: The cDNA was synthesized using reverse transcriptase (Sigma-Aldrich) after the RNA quality was verified by a spectrometer.

    Article Title: Metabolic profiling of Lantana camara L. using UPLC-MS/MS and revealing its inflammation-related targets using network pharmacology-based and molecular docking analyses
    Article Snippet: Dimethyl sulfoxide (DMSO), piroxicam, lipopolysaccharides (LPS), SYBR green master mix, trypan blue and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) dyes, RNA and cDNA extraction kits, nuclease free water, RNase inhibitor and reverse transcriptase were bought from Sigma (St. Louis, Mo., USA).

    Article Title: Vertical flow molecular assay apparatus
    Article Snippet: This kit has been developed to amplify DNA, but we add reverse transcriptase (AMV reverse transcriptase, Sigma-Aldrich, efficient at 42° C.) to the reaction mix in order to amplify RNA.

    Article Title: Treatment of alphavirus-induced inflammation
    Article Snippet: Extracted total RNA (20 ng/μL) was reverse-transcribed using an oligo (dT) primer and reverse transcriptase (Sigma Aldrich, Sydney, Australia) according to the manufacturer's instructions.

    Reverse Transcription:

    Article Title: Extracellular vesicles originating from steatotic hepatocytes promote hepatic stellate cell senescence via AKT/mTOR signaling.
    Article Snippet: RNA was isolated using Tri‐reagent (Sigma‐Aldrich) according to the manufacturer's protocol. .. RNA concentrations were measured by Nano‐Drop 2000c (Thermo Fisher Scientific) and 2 μg of RNA was used for reverse transcription (Sigma‐Aldrich). cDNA was diluted in RNAse‐free water and used for real‐time polymerase chain reaction on the QuantStudioTM 3 system (Thermo Fisher Scientific). ..

    Article Title: A defective splicing machinery promotes senescence through MDM4 alternative splicing.
    Article Snippet: The RNA integrity and concentration were monitored with an Agilent 2100 Bioanalyzer or with a spectrophotometer Nanodrop 2000. .. Reverse transcription was performed with the Transcriptor Reverse Transcriptase enzyme (Millipore Sigma, catalog no. 3531287001), random hexamers, dNTPs and 10 U of RNAse inhibitor RNAseOUT (Invitrogen, catalog no. 10777019) in reactions of 10 μL following manufacturer's instructions. ..

    Article Title: Association between Regulator of G Protein Signaling 9–2 and Body Weight
    Article Snippet: After 48 hr the cells were harvested and total cellular RNA was isolated using TRIzol reagent (according to manufacturer's instructions, Invitrogen). .. Next, the RNA was reverse transcribed (reverse transcriptase enzyme, Sigma Aldrich) at 37 °C for 50 min in a reaction that was primed with random hexamer nucleotides and contained dNTP mixture and RNase inhibitor (Ambion) and buffer provided by the enzyme supplier. .. The resulting cDNA was amplified in a PCR reactions (25 cycles) using Taq DNA polymerase (Promega, Madison, WI) and forward (5′ AAATGGAATCCATCCCGG 3′) and reverse (5′ AAACCCTAGAATCCTCATC 3′) primers flanking the first and last exon of the minigene such that splicing alterations resulting from exon skipping or intron retention would alter the size of the PCR fragment amplified.

    Real-time Polymerase Chain Reaction:

    Article Title: Extracellular vesicles originating from steatotic hepatocytes promote hepatic stellate cell senescence via AKT/mTOR signaling.
    Article Snippet: RNA was isolated using Tri‐reagent (Sigma‐Aldrich) according to the manufacturer's protocol. .. RNA concentrations were measured by Nano‐Drop 2000c (Thermo Fisher Scientific) and 2 μg of RNA was used for reverse transcription (Sigma‐Aldrich). cDNA was diluted in RNAse‐free water and used for real‐time polymerase chain reaction on the QuantStudioTM 3 system (Thermo Fisher Scientific). ..

    Random Hexamer:

    Article Title: Association between Regulator of G Protein Signaling 9–2 and Body Weight
    Article Snippet: After 48 hr the cells were harvested and total cellular RNA was isolated using TRIzol reagent (according to manufacturer's instructions, Invitrogen). .. Next, the RNA was reverse transcribed (reverse transcriptase enzyme, Sigma Aldrich) at 37 °C for 50 min in a reaction that was primed with random hexamer nucleotides and contained dNTP mixture and RNase inhibitor (Ambion) and buffer provided by the enzyme supplier. .. The resulting cDNA was amplified in a PCR reactions (25 cycles) using Taq DNA polymerase (Promega, Madison, WI) and forward (5′ AAATGGAATCCATCCCGG 3′) and reverse (5′ AAACCCTAGAATCCTCATC 3′) primers flanking the first and last exon of the minigene such that splicing alterations resulting from exon skipping or intron retention would alter the size of the PCR fragment amplified.



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