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precise whole transcriptome assay analysis pipeline v2.0  (Becton Dickinson)

 
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    Becton Dickinson precise whole transcriptome assay analysis pipeline v2.0
    Differential effects of BRAF V600E or KRAS G12V on gene expression and intestinal cell hierarchies. All panels: t-SNE visualisations and clustering of organoid single-cell transcriptomes clustered with k-means, 24 h after induction of FLUC control, BRAF V600E or KRAS G12V transgenes. a Colour code for six k-means clusters, and inferred differentiation trajectories starting at cluster 1 shown as grey overlay. b Colour code for transgene and CD44 positivity, as inferred from flow cytometry. CD44 positivity was used to direct cell selection, and thus relative fractions of CD44-high and -low cells are not representative. For CD44 status of the cell populations, see Supplementary Fig. . c Mapping of cell- and pathway-specific differentiation signatures. Numbers of signature genes detected are given per single-cell <t>transcriptome</t>
    Precise Whole Transcriptome Assay Analysis Pipeline V2.0, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptomic+analysis/pmc06606648-310-8-7?v=Becton+Dickinson
    Average 90 stars, based on 1 article reviews
    precise whole transcriptome assay analysis pipeline v2.0 - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium"

    Article Title: Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium

    Journal: Nature Communications

    doi: 10.1038/s41467-019-10954-y

    Differential effects of BRAF V600E or KRAS G12V on gene expression and intestinal cell hierarchies. All panels: t-SNE visualisations and clustering of organoid single-cell transcriptomes clustered with k-means, 24 h after induction of FLUC control, BRAF V600E or KRAS G12V transgenes. a Colour code for six k-means clusters, and inferred differentiation trajectories starting at cluster 1 shown as grey overlay. b Colour code for transgene and CD44 positivity, as inferred from flow cytometry. CD44 positivity was used to direct cell selection, and thus relative fractions of CD44-high and -low cells are not representative. For CD44 status of the cell populations, see Supplementary Fig. . c Mapping of cell- and pathway-specific differentiation signatures. Numbers of signature genes detected are given per single-cell transcriptome
    Figure Legend Snippet: Differential effects of BRAF V600E or KRAS G12V on gene expression and intestinal cell hierarchies. All panels: t-SNE visualisations and clustering of organoid single-cell transcriptomes clustered with k-means, 24 h after induction of FLUC control, BRAF V600E or KRAS G12V transgenes. a Colour code for six k-means clusters, and inferred differentiation trajectories starting at cluster 1 shown as grey overlay. b Colour code for transgene and CD44 positivity, as inferred from flow cytometry. CD44 positivity was used to direct cell selection, and thus relative fractions of CD44-high and -low cells are not representative. For CD44 status of the cell populations, see Supplementary Fig. . c Mapping of cell- and pathway-specific differentiation signatures. Numbers of signature genes detected are given per single-cell transcriptome

    Techniques Used: Expressing, Flow Cytometry, Selection



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    Image Search Results


    Effects of Different HLP Concentrations on the Relative Expression Levels of DEGs. (A) qPCR Validation of Transcriptomic Results for Peripheral Blood Lymphocytes. (B) qPCR Validation of Transcriptomic Results for Duodenal Tissues. Statistical tests were used for comparisons between groups, where “ns” indicates no significant difference, “**” indicates P < 0.01, and “****” indicates P < 0.0001. Error bars represent the range of mean variation (e.g., standard deviation).

    Journal: Poultry Science

    Article Title: Analysis of the immunomodulatory effects of Honeysuckle leaf polysaccharides on cherry valley ducks based on transcriptomic techniques

    doi: 10.1016/j.psj.2026.106737

    Figure Lengend Snippet: Effects of Different HLP Concentrations on the Relative Expression Levels of DEGs. (A) qPCR Validation of Transcriptomic Results for Peripheral Blood Lymphocytes. (B) qPCR Validation of Transcriptomic Results for Duodenal Tissues. Statistical tests were used for comparisons between groups, where “ns” indicates no significant difference, “**” indicates P < 0.01, and “****” indicates P < 0.0001. Error bars represent the range of mean variation (e.g., standard deviation).

    Article Snippet: Simultaneously, the liver, spleen, and duodenum were collected, rapidly frozen in liquid nitrogen, stored at −80°C, and subsequently sent to Novogene Bio-Technology Co., Ltd. for transcriptomic analysis ( ).

    Techniques: Expressing, Biomarker Discovery, Standard Deviation