gene exp ins ss03386682 u1 (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher gene exp ins ss03386682 u1

( A , B ) UMAPs showing the cell types/states ( A ) and sample composition ( B ) of the integrated scRNA-Seq data from two PPDO lines (1 LPN and 1 Ad) at passage 4 and 7. ( C ) Dot plot showing expression of the top 5 marker genes of each PPDO cluster. ( D ) Dot plot showing expression of key pancreatic gene markers for major cell types of the HPDO scRNA-Seq dataset including ductal ( KRT19 - SLC4A4 - BMPR1A - MUC1 - SOX9 ), endocrine ( <t>INS</t> - CHGB ), acinar/pancreatic progenitor ( GP2 ), acinar ( RBPJL - CELA1 ) endothelial ( PECAM1 - FLT1 ), stellate ( COL1A1 ), immune ( PTPRC ), and schwann ( CDH19 ) cell markers. ( E ) UMAP showing the cell types/states of scRNA-Seq data from HPDO. ( F ) UMAP showing the cross-species comparison of the integrated PPDO-HPDO scRNA-Seq datasets. ( G – L ) Dot plots of expression of important genes from the WNT ( G , H ), NOTCH ( I , J ) and Hippo ( K , L ) signaling pathways in the scRNA-Seq dataset of the integrated PPDO ( G , I , K ), and HPDO ( H , J , L ).

Gene Exp Ins Ss03386682 U1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transcriptome+sequencing+experiment/Gene+Exp%2E+INS%2C+Ss03386682_u1/pmc12686538-557-25--1
Average 94 stars, based on 1 article reviews

gene exp ins ss03386682 u1 - by Bioz Stars, 2026-10

94/100 stars

Images

1) Product Images from "Benchmarking porcine pancreatic ductal organoids for drug screening applications"

Article Title: Benchmarking porcine pancreatic ductal organoids for drug screening applications

Journal: EMBO Molecular Medicine

doi: 10.1038/s44321-025-00330-3

Figure Legend Snippet: ( A , B ) UMAPs showing the cell types/states ( A ) and sample composition ( B ) of the integrated scRNA-Seq data from two PPDO lines (1 LPN and 1 Ad) at passage 4 and 7. ( C ) Dot plot showing expression of the top 5 marker genes of each PPDO cluster. ( D ) Dot plot showing expression of key pancreatic gene markers for major cell types of the HPDO scRNA-Seq dataset including ductal ( KRT19 - SLC4A4 - BMPR1A - MUC1 - SOX9 ), endocrine ( INS - CHGB ), acinar/pancreatic progenitor ( GP2 ), acinar ( RBPJL - CELA1 ) endothelial ( PECAM1 - FLT1 ), stellate ( COL1A1 ), immune ( PTPRC ), and schwann ( CDH19 ) cell markers. ( E ) UMAP showing the cell types/states of scRNA-Seq data from HPDO. ( F ) UMAP showing the cross-species comparison of the integrated PPDO-HPDO scRNA-Seq datasets. ( G – L ) Dot plots of expression of important genes from the WNT ( G , H ), NOTCH ( I , J ) and Hippo ( K , L ) signaling pathways in the scRNA-Seq dataset of the integrated PPDO ( G , I , K ), and HPDO ( H , J , L ).

Techniques Used: Expressing, Marker, Comparison, Protein-Protein interactions

( A , B ) Brightfield microscopy images of PPDO in complete ( A ) or porcine serum supplemented ( B ). Scale bar 500 µm. ( C , D ) Brightfield microscopy images of PPDO at the end of differentiation in complete ( C ) or at the end of the differentiation using S5 + S6 combination ( D ) media. Scale bar 500 µm. ( E –J ) Bar plots showing the fold change of gene expression analysis at the end of the differentiation after treatment with porcine serum. Gene expression was measured for CFTR ( E ), KRT7 ( F ), NEUROD1 ( G ), GCG ( H ), INS ( I ), and SST ( J ). n = 3 independent PPDO lines (1 Em and 2 EPN). Absence of samples from the plots indicate non-detectable amplification following the RT-PCR. Data are shown as mean ± SEM. Unpaired Student’s t-test was used to assess significance with * P = 0.0196 for NEUROD1 . ( K , L ) Single-plane confocal images of PPDO in growth media ( K ) or in differentiation media from Loomans et al ( L ) (see Methods). PPDO were immunostained against INS-KRT7-CHGA and counterstained with DAPI. Experiment was repeated with n = 3 biological replicates (1 Em-1 EPN-1 Ad). Scale bar: 50 µm. ( M , N ) Brightfield microscopy images of HPDO in differentiation media ( M ) or differentiation media supplemented with DAPT/DEAB small molecules ( N ). Experiment was repeated with n = 3 biological replicates. Scale bar 500 µm.

Figure Legend Snippet: ( A , B ) Brightfield microscopy images of PPDO in complete ( A ) or porcine serum supplemented ( B ). Scale bar 500 µm. ( C , D ) Brightfield microscopy images of PPDO at the end of differentiation in complete ( C ) or at the end of the differentiation using S5 + S6 combination ( D ) media. Scale bar 500 µm. ( E –J ) Bar plots showing the fold change of gene expression analysis at the end of the differentiation after treatment with porcine serum. Gene expression was measured for CFTR ( E ), KRT7 ( F ), NEUROD1 ( G ), GCG ( H ), INS ( I ), and SST ( J ). n = 3 independent PPDO lines (1 Em and 2 EPN). Absence of samples from the plots indicate non-detectable amplification following the RT-PCR. Data are shown as mean ± SEM. Unpaired Student’s t-test was used to assess significance with * P = 0.0196 for NEUROD1 . ( K , L ) Single-plane confocal images of PPDO in growth media ( K ) or in differentiation media from Loomans et al ( L ) (see Methods). PPDO were immunostained against INS-KRT7-CHGA and counterstained with DAPI. Experiment was repeated with n = 3 biological replicates (1 Em-1 EPN-1 Ad). Scale bar: 50 µm. ( M , N ) Brightfield microscopy images of HPDO in differentiation media ( M ) or differentiation media supplemented with DAPT/DEAB small molecules ( N ). Experiment was repeated with n = 3 biological replicates. Scale bar 500 µm.

Techniques Used: Microscopy, Gene Expression, Amplification, Reverse Transcription Polymerase Chain Reaction

( A ) Volcano plot indicating the number of significantly changed genes following the 7 days of culturing in the basal differentiation medium. Genes important for developmental processes are highlighted in the graph. Bulk RNA-Seq was performed on 4 biological replicate PPDO from 1 Em, 2 EPN and 1 LPN samples. Statistical analysis was employed as described in DESEQ2 package using a Wald test with Benjamin and Hochberg adjustment for multiple testing. ( B , C ) Bar plots showing the fold enrichment of significantly affected biological processes for the upregulated and downregulated genes of the RNA-Seq dataset shown in ( A ). ( B ) shows selected enriched GO terms for the significantly upregulated genes and ( C ) shows selected GO terms for the significantly downregulated genes. ( D – G ) Plots showing the fold change of gene expression for KRT7 ( D ), NEUROD1 ( E ), INS ( F ) and GCG ( G ) genes between differentiation medium alone or supplemented with DAPT-DEAB-BMS754807. Data are shown as mean ± SEM. Mann–Whitney test was used to assess significance with ** P = 0.0043 for NEUROD1 , ns P = 0.1508 for INS and ns ns P = 0.3095 for GCG . n = 6 different PPDO lines were assayed (2 Em, 3 EPN, 1 Ad). ( H – K ) Single-plane confocal images of PPDO derived from Em pig pancreas from passage 2 ( H , I ) or passage 6 ( J , K ) cultures and treated with differentiation only ( H – J ) or differentiation media supplemented with the NOTCH inhibitor DAPT and the aldehyde dehydrogenase inhibitor DEAB ( I – K ). PPDO were immunostained against KRT7 (gray)-INS/GCG (magenta) and counterstained with DAPI. Arrowheads in I indicate the insulin positive cells. Similar staining patterns were obtained from n = 4 different biological replicate PPDO lines (1 Em, 2 EPN, 1 Ad). Scale bar: 50 µm. .

Figure Legend Snippet: ( A ) Volcano plot indicating the number of significantly changed genes following the 7 days of culturing in the basal differentiation medium. Genes important for developmental processes are highlighted in the graph. Bulk RNA-Seq was performed on 4 biological replicate PPDO from 1 Em, 2 EPN and 1 LPN samples. Statistical analysis was employed as described in DESEQ2 package using a Wald test with Benjamin and Hochberg adjustment for multiple testing. ( B , C ) Bar plots showing the fold enrichment of significantly affected biological processes for the upregulated and downregulated genes of the RNA-Seq dataset shown in ( A ). ( B ) shows selected enriched GO terms for the significantly upregulated genes and ( C ) shows selected GO terms for the significantly downregulated genes. ( D – G ) Plots showing the fold change of gene expression for KRT7 ( D ), NEUROD1 ( E ), INS ( F ) and GCG ( G ) genes between differentiation medium alone or supplemented with DAPT-DEAB-BMS754807. Data are shown as mean ± SEM. Mann–Whitney test was used to assess significance with ** P = 0.0043 for NEUROD1 , ns P = 0.1508 for INS and ns ns P = 0.3095 for GCG . n = 6 different PPDO lines were assayed (2 Em, 3 EPN, 1 Ad). ( H – K ) Single-plane confocal images of PPDO derived from Em pig pancreas from passage 2 ( H , I ) or passage 6 ( J , K ) cultures and treated with differentiation only ( H – J ) or differentiation media supplemented with the NOTCH inhibitor DAPT and the aldehyde dehydrogenase inhibitor DEAB ( I – K ). PPDO were immunostained against KRT7 (gray)-INS/GCG (magenta) and counterstained with DAPI. Arrowheads in I indicate the insulin positive cells. Similar staining patterns were obtained from n = 4 different biological replicate PPDO lines (1 Em, 2 EPN, 1 Ad). Scale bar: 50 µm. .

Techniques Used: RNA Sequencing, Gene Expression, MANN-WHITNEY, Derivative Assay, Staining

Gene Expression:

Article Title: Directed self-assembly of a xenogeneic vascularized endocrine pancreas for type 1 diabetes.
Article Snippet: .. Pig gene expression in NPIs and VEPs To evaluate the endocrine maturation of NPIs in the VEP device, we performed gene expression experiments quantifying by ddPCR the pig insulin (Ss03386682_u1, ThermoFisher Scientific, Waltham, USA), glucagon (Ss03384069_u1, ThermoFisher Scientific, Waltham, USA), somatostatin (Ss03391856_m1, ThermoFisher Scientific, Waltham, USA) and gapdh (Ss03375629_u1, ThermoFisher Scientific, Waltham, USA) transcripts8 at day 1 and day 7. .. The probe assays targeting the pig insulin and the human GAPDH (Hs04420697_g1, ThermoFisher Scientific, Waltham, USA) genes were also used to quantify the pig and human DNA, respectively, and to normalize the gene expression results based on the cell content of each VEP lysate.

Article Title: Directed self-assembly of a xenogeneic vascularized endocrine pancreas for type 1 diabetes
Article Snippet: .. To evaluate the endocrine maturation of NPIs in the VEP device, we performed gene expression experiments quantifying by ddPCR the pig insulin (Ss03386682_u1, ThermoFisher Scientific, Waltham, USA), glucagon (Ss03384069_u1, ThermoFisher Scientific, Waltham, USA), somatostatin (Ss03391856_m1, ThermoFisher Scientific, Waltham, USA) and gapdh (Ss03375629_u1, ThermoFisher Scientific, Waltham, USA) transcripts at day 1 and day 7. .. The probe assays targeting the pig insulin and the human GAPDH (Hs04420697_g1, ThermoFisher Scientific, Waltham, USA) genes were also used to quantify the pig and human DNA, respectively, and to normalize the gene expression results based on the cell content of each VEP lysate.

other:

Article Title: Benchmarking porcine pancreatic ductal organoids for drug screening applications.
Article Snippet: RNAwas extracted using the Arcturus PicoPure RNA isolation kit (Applied Biosystems) and eluted with 15 μl Nucleic-acid free water according to manufacturer’s instructions.

Article Title: Optimization of an O 2 -balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment
Article Snippet: Insulin , INS , Ss03386682_u1.

Article Title: Xenotransplantation of tannic acid-encapsulated neonatal porcine islets decreases proinflammatory innate immune responses.
Article Snippet: 1Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, USA 2Comprehensive Diabetes Center, University of Alabama at Birmingham, Birmingham, AL, USA 3Department of Chemistry, University of Alabama at Birmingham, Birmingham, AL, USA 4Center for Nanoscale Materials and Biointegration, University of Alabama at Birmingham, Birmingham, AL, USA 5Department of Medicine, Division of Endocrinology Diabetes and Metabolism, University of Alabama at Birmingham, Birmingham, AL, USA 6Department of Surgery, Alberta Diabetes Institute, University of Alberta, Edmonton, AB, Canada

Sequencing:

Article Title: Benchmarking porcine pancreatic ductal organoids for drug screening applications.
Article Snippet: .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models Primary porcine ductal organoids N/A N/A Primary human ductal organoids N/A N/A Wild-type Landrace Pigs N/A N/A INS-eGFP Landrace Pigs N/A N/A Antibodies Rabbit a-KRT5 Abcam ab53121 Rabbit a-SOX9 Millipore AB3555 Mouse a-KRT7 Agilent Technologies M701829-2 Guinea pig a-KRT8/18 Origene BP5007 Rabbit a-PAN-KRT Agilent Technologies Z0622 Reagent/Resource Reference or Source Identifier or Catalog Number Mouse a-BMPR1A LSBio LS-C191759 Mouse a-CDH1 BD Biosciences 610181 Rat a-CDH1 Takara M108 Rabbit a-CFTR Cell Signaling 78335 Mouse a-GP2 MBL Life Science D277-3 Goat a-PDX1 R&D Systems AF2419 Rabbit a-AGR2 Cell Signaling 13062S Mouse a-GCG Sigma-Aldrich G2654 Mouse a-NKX6-1 Developmental Studies Hybridoma Bank F55A10 Sheep a-NEUROG3 R&D Systems AF3444 Rabbit a-phospho (Ser10) histone 3 Cell Signaling 3377S Rabbit a-pancreatic amylase Abcam 21156 Guinea pig a-INS LSBio LS-C85862-1 Guinea pig a-GCG Takara M182 Rabbit a-cleaved-caspase3ASP175 Cell Signaling 9661S Phalloidin Alexa Fluor 546 Invitrogen A22283 a-Ki670 Vio G570 Miltenyi Biotec 130-133-796 Streptavidin Alexa Fluor 647 Life Technologies S21374 Multiple Alexa Fluor conjugated secondary antibodies ThermoFischer Scientific N/A Oligonucleotides and other sequence-based reagents INS TaqMan probe ThermoFischer Scientific Ss03386682_u1 GCG TaqMan probe ThermoFischer Scientific Ss03384069_u1 NEUROD1 TaqMan probe ThermoFischer Scientific Ss03373333_s1 SST TaqMan probe ThermoFischer Scientific Ss03391856_m1 ACTB TaqMan probe ThermoFischer Scientific Ss03376563_Uh KRT7 TaqMan probe ThermoFischer Scientific Ss06890421_Mh CFTR TaqMan probe ThermoFischer Scientific Ss03389420_m1 Chemicals, enzymes and other reagents Collagenase V Merck-Millipore C9263 HBSS Gibco 24020-091 TrypLE Express 1X Gibco 12605-010 Advanced DMEM/F12 Life Technologies 12634-010 Penicillin-Streptomycin Gibco 15140163 Primocin InvivoGen ant-pm-2 GlutaMAX ThermoFischer Scientific 35050038 N-2 supplement ThermoFischer Scientific 17502048 © The Author(s) EMBO Molecular Medicine 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 182.10.130.72. .. Reagents and tools table Reagent/Resource Reference or Source Identifier or Catalog Number Experimental models Primary porcine ductal organoids N/A N/A Primary human ductal organoids N/A N/A Wild-type Landrace Pigs N/A N/A INS-eGFP Landrace Pigs N/A N/A Antibodies Rabbit a-KRT5 Abcam ab53121 Rabbit a-SOX9 Millipore AB3555 Mouse a-KRT7 Agilent Technologies M701829-2 Guinea pig a-KRT8/18 Origene BP5007 Rabbit a-PAN-KRT Agilent Technologies Z0622 Reagent/Resource Reference or Source Identifier or Catalog Number Mouse a-BMPR1A LSBio LS-C191759 Mouse a-CDH1 BD Biosciences 610181 Rat a-CDH1 Takara M108 Rabbit a-CFTR Cell Signaling 78335 Mouse a-GP2 MBL Life Science D277-3 Goat a-PDX1 R&D Systems AF2419 Rabbit a-AGR2 Cell Signaling 13062S Mouse a-GCG Sigma-Aldrich G2654 Mouse a-NKX6-1 Developmental Studies Hybridoma Bank F55A10 Sheep a-NEUROG3 R&D Systems AF3444 Rabbit a-phospho (Ser10) histone 3 Cell Signaling 3377S Rabbit a-pancreatic amylase Abcam 21156 Guinea pig a-INS LSBio LS-C85862-1 Guinea pig a-GCG Takara M182 Rabbit a-cleaved-caspase3ASP175 Cell Signaling 9661S Phalloidin Alexa Fluor 546 Invitrogen A22283 a-Ki670 Vio G570 Miltenyi Biotec 130-133-796 Streptavidin Alexa Fluor 647 Life Technologies S21374 Multiple Alexa Fluor conjugated secondary antibodies ThermoFischer Scientific N/A Oligonucleotides and other sequence-based reagents INS TaqMan probe ThermoFischer Scientific Ss03386682_u1 GCG TaqMan probe ThermoFischer Scientific Ss03384069_u1 NEUROD1 TaqMan probe ThermoFischer Scientific Ss03373333_s1 SST TaqMan probe ThermoFischer Scientific Ss03391856_m1 ACTB TaqMan probe ThermoFischer Scientific Ss03376563_Uh KRT7 TaqMan probe ThermoFischer Scientific Ss06890421_Mh CFTR TaqMan probe ThermoFischer Scientific Ss03389420_m1 Chemicals, enzymes and other reagents Collagenase V Merck-Millipore C9263 HBSS Gibco 24020-091 TrypLE Express 1X Gibco 12605-010 Advanced DMEM/F12 Life Technologies 12634-010 Penicillin-Streptomycin Gibco 15140163 Primocin InvivoGen ant-pm-2 GlutaMAX ThermoFischer Scientific 35050038 N-2 supplement ThermoFischer Scientific 17502048 © The Author(s) EMBO Molecular Medicine 15 on N ovem ber 8, 2025 from IP 182.10.130.72

Reverse Transcription Polymerase Chain Reaction:

Article Title: Benchmarking porcine pancreatic ductal organoids for drug screening applications
Article Snippet: Library preparation and sequencing were performed at Helmholtz Zentrum München (HMGU) by the Genomics Core Facility. .. RT-PCR was performed with 0.5 μl of initial RNA using the TaqMan Fast Advanced Mix and the following TaqMan probes coupled with 6-carboxyfluorescein (FAM): INS (Ss03386682_u1), GCG (Ss03384069_u1), NEUROD1 (Ss03373333_s1), SST (Ss03391856_m1), ACTB (Ss03376563_Uh), KRT7 (Ss06890421_Mh), and CFTR (Ss03389420_m1). ..

Review